Bacterial blight (BB) is one of the major dis-eases to rice. Antibacterial Cecropin B genehas been cloned and transformed into rice. Westudied the resistance to bacterial blight inCecropin B gene transgenic rices.Rice...Bacterial blight (BB) is one of the major dis-eases to rice. Antibacterial Cecropin B genehas been cloned and transformed into rice. Westudied the resistance to bacterial blight inCecropin B gene transgenic rices.Rice variety JYll9 transformed withCecropin B gene by particle bombardment andprogenies were randomly planted in the field in展开更多
An expression vector pCBl suitable for rice transformation, harboring a synthesized cecropin B gene and a selectable marker gene (bar), was constructed. It was introduced into immature embryos of two japonica varietie...An expression vector pCBl suitable for rice transformation, harboring a synthesized cecropin B gene and a selectable marker gene (bar), was constructed. It was introduced into immature embryos of two japonica varieties by particle bombardment, and several transgenic plants were obtained. The results from Basta treatment, PCR analysis, dot and Southern blot analysis of cecropin B gene in transgenic plants indicated that both bar and cecropin B gene were integrated into the genome of transformed plants. Northern blot analysis of transgenic plants showed the expression of cecropin B gene at transcriptional level. Some of transgenic plants revealed improved resistances to two types of bacterial diseases, rice bacterial blight and rice bacterial streak to different extent.展开更多
文摘Bacterial blight (BB) is one of the major dis-eases to rice. Antibacterial Cecropin B genehas been cloned and transformed into rice. Westudied the resistance to bacterial blight inCecropin B gene transgenic rices.Rice variety JYll9 transformed withCecropin B gene by particle bombardment andprogenies were randomly planted in the field in
基金Project supported by the Eighth Five-Year-Plan and the Rockefeller Foundation.
文摘An expression vector pCBl suitable for rice transformation, harboring a synthesized cecropin B gene and a selectable marker gene (bar), was constructed. It was introduced into immature embryos of two japonica varieties by particle bombardment, and several transgenic plants were obtained. The results from Basta treatment, PCR analysis, dot and Southern blot analysis of cecropin B gene in transgenic plants indicated that both bar and cecropin B gene were integrated into the genome of transformed plants. Northern blot analysis of transgenic plants showed the expression of cecropin B gene at transcriptional level. Some of transgenic plants revealed improved resistances to two types of bacterial diseases, rice bacterial blight and rice bacterial streak to different extent.
