Using an engineering perspective which is looking at the hair cells cilia as a total entity interacting with its neighboring cilia along their entire length, a theory of capacitive transduction is developed. This larg...Using an engineering perspective which is looking at the hair cells cilia as a total entity interacting with its neighboring cilia along their entire length, a theory of capacitive transduction is developed. This larger scaled view of cilia interaction suggests that transduction may be achieved through a mechanically controlled variable capacitor in the cilia bundle. A brief review of some seldom considered facts about electrical capacitors supports the hypothesis presented. Experimental measurements of hair cell reversal potential and ionic conditions surrounding the cilia during transduction, long reported in the biophysical literature, also support a capacitive hypothesis of transduction.展开更多
目的：建立水合氯醛去除成骨细胞初级纤毛的细胞模型,并观察去除成骨细胞初级纤毛对电磁场提高成骨细胞ALP染色和钙化结节染色的影响。方法：贴壁法分离培养3只出生3 d,体重6~9 g的雄性SD大鼠的乳鼠颅骨成骨细胞;待上述细胞生长至融合...目的：建立水合氯醛去除成骨细胞初级纤毛的细胞模型,并观察去除成骨细胞初级纤毛对电磁场提高成骨细胞ALP染色和钙化结节染色的影响。方法：贴壁法分离培养3只出生3 d,体重6~9 g的雄性SD大鼠的乳鼠颅骨成骨细胞;待上述细胞生长至融合状态时传代培养并随机分成4组：不加水合氯醛组（对照组）、2 m M、4 m M和8 m M水合氯醛处理组;将上述4组细胞置于37℃、5%CO2的培养箱培养72 h,用激光共聚焦扫描显微镜观察初级纤毛形态,并用Image-Pro Plus 6.0软件分析成骨细胞初级纤毛发生率;筛选出能有效去除成骨细胞初级纤毛的水合氯醛浓度的细胞,并将其分为以下3组：正常对照组（control group,C）,电磁场处理（electromagnetic fields,EMFs）组及电磁场处理＋4 m M水合氯醛组。向上述3组细胞加入含10%FBS的DMEM培养液继续培养9 d,碱性磷酸酶组织化学染色观察ALP的形成;培养12 d,茜素红染色观察钙化结节的形成。结果：与对照组和2 m M水合氯醛组相比,4 m M水合氯醛能有效地去除成骨细胞初级纤毛的发生（P〈0.01）;去除成骨细胞初级纤毛消弱了EMFs促进成骨细胞ALP和钙化结节的形成,具体表现在：与EMFs组相比,EMFs＋水合氯醛组ALP和钙化结节的染色面积明显减少（P〈0.01）。结论：4 m M水合氯醛能有效去除成骨细胞的初级纤毛;初级纤毛部分参与了电磁场促进成骨细胞ALP和钙化结节的形成,为探讨电磁场促进成骨细胞成熟矿化的机理提供新思路。展开更多
文摘Using an engineering perspective which is looking at the hair cells cilia as a total entity interacting with its neighboring cilia along their entire length, a theory of capacitive transduction is developed. This larger scaled view of cilia interaction suggests that transduction may be achieved through a mechanically controlled variable capacitor in the cilia bundle. A brief review of some seldom considered facts about electrical capacitors supports the hypothesis presented. Experimental measurements of hair cell reversal potential and ionic conditions surrounding the cilia during transduction, long reported in the biophysical literature, also support a capacitive hypothesis of transduction.
文摘目的：建立水合氯醛去除成骨细胞初级纤毛的细胞模型,并观察去除成骨细胞初级纤毛对电磁场提高成骨细胞ALP染色和钙化结节染色的影响。方法：贴壁法分离培养3只出生3 d,体重6~9 g的雄性SD大鼠的乳鼠颅骨成骨细胞;待上述细胞生长至融合状态时传代培养并随机分成4组：不加水合氯醛组（对照组）、2 m M、4 m M和8 m M水合氯醛处理组;将上述4组细胞置于37℃、5%CO2的培养箱培养72 h,用激光共聚焦扫描显微镜观察初级纤毛形态,并用Image-Pro Plus 6.0软件分析成骨细胞初级纤毛发生率;筛选出能有效去除成骨细胞初级纤毛的水合氯醛浓度的细胞,并将其分为以下3组：正常对照组（control group,C）,电磁场处理（electromagnetic fields,EMFs）组及电磁场处理＋4 m M水合氯醛组。向上述3组细胞加入含10%FBS的DMEM培养液继续培养9 d,碱性磷酸酶组织化学染色观察ALP的形成;培养12 d,茜素红染色观察钙化结节的形成。结果：与对照组和2 m M水合氯醛组相比,4 m M水合氯醛能有效地去除成骨细胞初级纤毛的发生（P〈0.01）;去除成骨细胞初级纤毛消弱了EMFs促进成骨细胞ALP和钙化结节的形成,具体表现在：与EMFs组相比,EMFs＋水合氯醛组ALP和钙化结节的染色面积明显减少（P〈0.01）。结论：4 m M水合氯醛能有效去除成骨细胞的初级纤毛;初级纤毛部分参与了电磁场促进成骨细胞ALP和钙化结节的形成,为探讨电磁场促进成骨细胞成熟矿化的机理提供新思路。
