外周血CD4^(+)PD-1^(+)Tcells及CD4^(+)T淋巴细胞ATP含量与复发性卵巢癌疗效的相关性分析

目的 探讨外周血CD4^(+)程序性细胞死亡受体-1(PD-1)^(+)T cells及CD4^(+)T淋巴细胞三磷酸腺苷(ATP)含量与复发性卵巢癌疗效的相关性。方法 选取30例复发性卵巢癌患者为复发组,30例未复发卵巢癌患者为非复发组;另选取30名同期体检健康者作为对照组。评估外周血CD4^(+)PD-1^(+)T cells及CD4^(+)T淋巴细胞ATP含量与复发性卵巢癌疗效的相关性。结果 复发组和非复发组的CD4^(+)PD-1^(+)T cells较对照组明显升高(P<0.05)。复发组和非复发组的CD4^(+)T淋巴细胞ATP含量较对照组明显降低(P<0.05)。复发组治疗后CD4^(+)PD-1^(+)Tcells显著低于治疗前(P<0.05),治疗后CD4^(+)T淋巴细胞ATP含量显著高于治疗前(P<0.05)。CD4^(+)PD-1^(+)T cells与复发性卵巢癌疗效成负相关(r=-0.393,P=0.039),CD4^(+)T淋巴细胞ATP含量与复发性卵巢癌疗效成正相关(r=0.449,P=0.031)。结论 复发性卵巢癌患者外周血CD4^(+)PD-1^(+)T cells及CD4^(+)T淋巴细胞ATP含量与疗效密切相关。

DNA Content in Pancreatic ExocrinalCells after Vagotom yand Electron Microscopy in Rats

Fifty six SD rats were randomly divided into the normal control group and the operation group. The operating group was subdivided into six groups on the basis of killing time (the 12th h, the first day, 3rd day, first week, 2nd week and 4th week) after vagotomy (VG). The pancreatic tissues were taken for HE and Feulgen staining. The DNA content of pancreatic exocrine cells was determined by a domestically fabricated computer image analyzing system. In the control group, on the first day or in the first week after VG, the pancreatic samples were taken for transmission electron microscopic examination. The DNA content of pancreatic exocrinal cells was decreased from 1 to 3 days after VG. The secretion was found to be in inhibitory state and One week later, it gradually restored. The results indicated that the proliferation and the function of the SD rat's pancreatic exocrinal cells were prohibited at initial stage after VG, which might be concerned, at least in part, with dominance and nutrition of vagus.

An Optimal Resources Configuration Scheme for Caching-Based Content Distribution in BackhaulLimited Small Cell Networks

In this paper, we study the problem of resource allocation in small cell networks with distributed caching, that is, how to divide the spectrum resources for backhaul and access in order to improve network performance. We formulate our concerned problem by using multi-dimensional Markov model. Our problem considers that each user may access multiple small cell base stations. We conduct simulation experiments to evaluate the performance of our proposed resource allocation scheme. Our simulation results show that the appropriate spectrum resource allocation is important to improve network performance.

Total Phenolic Content and Antioxidant Activity of Standardized Extracts from Leaves and Cell Cultures of Three Callistemon Species

A comparative study was carried out with ethanolic (80%) extracts from leaves and cell cultures of three Callistemon species, namely C. lanceolatus (CL), C. viridiflorous (CV), and C. comboynensis (CC). Cell suspensions of the three species were grown in liquid Murashige and Skoog (MS) medium (100 ml) supplemented with 0.9 mg·g-1 kinetin in combination with 1.1 mg·g-1 NAA. The CL leaf extract was standardized to contain the highest amount of phenolics (104 ± 2.0 mg·g-1), followed by CC (95.8 ± 1.2 mg·g-1) and CV (79.8 ± 4.6 mg·g-1). On the other hand, cell cultures of CV contained more phenolics (14.9 ± 0.6 mg·g-1) than those of the other two species, CL and CC, which contained 12.2 ± 0.16 and 9.12 ± 0.16 mg·g-1, respectively. Nevertheless, CV leaf extract exhibited the highest antioxidant activity (91.4% ± 0.4%) at a concentration of 1000 μg·ml-1, comparable to 100 μg·ml-1 gallic acid (90.8% ± 1.5%).

GaN As/InGaAs Superlattice Solar Cells with High N Content in the Barrier Grown by All Solid-State Molecular Beam Epitaxy

We demonstrate nearly i e V GaN0.03As0.97 /In0.09 Ga0.91As strain-compensated short-period superlattice solar cells by all solid-state molecular beam epitaxy. The optimal period thickness for the superlattice growth is achieved to realize high structural quality. Meanwhile, the annealing conditions are optimized to realize a pho- toluminescence （PL） at a low temperature. However, no PL signal is detected at room temperature, which could be reflected by a lower open-circuit voltage of the fabricated devices. The GaN0.03As0.97/In0.09Ga0.91As super- lattice solar cells show a reasonably-high short-circuit current density （Jsc） of over lOmA/cm2. Eurthermore, a concentration behavior is measured, which shows a linear relationship between Jsc and concentration ratios. The extrapolated ideality factor and saturated current density by the concentration action are in good agreement with that extracted by the dark case of the p-i-n diodes.

QUANTITATIVE STUDIES ON THE DNA CONTENT AND MORPHOMETRIC FEATURES OF LIVER CELL DYSPLASIA

The DNA content and morphometric features of hepatocellular carcinoma (HCC) and liver cell dysplasia (LCD), including nuclear area, nuclear perimeter, nuclear maximum diameter and nuclear circle diameter, were quantitatively determined by means of image analysis technology. The results showed that in comparison with normal hepatocytes, LCD had a markedly increased DNA content and nuclear morphometric parameters, but the values were lower than those for HCC. LCD showed a slight increase in nuclear atypia represented by the nuclear irregular index, which was also less than HCC. The findings indicate that LCD may be a precaneerous lesion of HCC, to the cells in an abnormal proliferative state.

Studies on Single Cell Culture in vitro in Wheat——The variation of grain protein content and its fractions from regenerated plants

On the basis of previous studies dealing with the variation of major agronomic and yield characteristics of regenerated plants derived from single cell culture in vitro of common wheat (Triticum aestivum L.Cultivar NE 7742), the grain protein content and its fractions from regenerated plants with stable agronomic characteristics were studied from 1992 to 1995. The results showed that the variation of grain protein content and its fractions in somaclones from single cell culture in vitro were very significant and the range was very wide (11531770%). Several types of variation were found in the studies, especially the type with higher protein content than that of cultivar NE 7742 (non-culture parent). Among them, -2069% of lines the grain protein content was significantly higher than that of NE 7742 and combined with high yielding potential. The tendency of variation of the four protein fractions showed that the variation of albumin was not obvious and maintained the same level as NE774 increased in some somaclones and decreased in others. However, the percentages both globulin and glutenin tended to increase. The variation of total amount of structural protein and the ratio between globulin and glutenin tended to increase. The variation of total amount of structural protein and the ratio between globulin and albumm was mainly influenced by globulin under the condition of culture in vitro. The variation of total amount of storage protein and the ratio between gliadin and glutenin was mainly affected by glutenin. The results mentioned above demonstrated that the induction and screening of somaclonal variation could be an effective way in wheat improvement in combining high protein content with high yield.

Udder Health Status of First Parity Dairy Cows in Early-Lactation Based on Somatic Cell Count Categories

The main aim of this study was to investigate the prevalence of intramammary infection (IMI) in early-lactation of primiparous cows using milk recording cow composite somatic cell count (CSCC) categories (combining the first 2 milk recording results after calving). Another aim was to evaluate the milk urea (MU) content as a potential supplementary indicator to SCC or CSCC for the identification of IMI in primiparous cows after calving. This retrospective observational study was conducted on records of test-day of primiparous cows over a period of 6 years (January 2016 to December 2021. The SCC data for 158 Holstein Friesian primiparous cows, with their first milk recording 5 to 35 days after calving and their second milk recording 28 to 56 days in milk (DIM), were identified. Each primiparous cow was assigned a CSCC category (low-low, low-high, high-low or high-high) based on the CSCC at the first 2 milking recordings using the following cut-offs: ≤150,000 cells/ml (low), >150,000 cells/ml (high). The association between CSCC categories and MV content was analyzed using correlation models. At the first milk recording, a proportion of 63.29% was in the low SCC category, and the rest (36.71%) was in the high SCC category. At the second milk recording, a proportion of primiparous cows in CSCC categories was 59.49%, 3.80%, 27.85% and 8.86% in low-low, low-high, high-low and high-high, respectively. At the second milk recording, a proportion of 12.66% of primiparous cows was in the high CSCC category and a proportion of 87.34% of primiparous cows was in the low CSCC category, indicating a poor and a good udder health, respectively. The association of SCC with MU content in low and in high SCC categories at the first milk recording was positive and moderate (+0.49) and negative and strong (-0.97), respectively. The association of CSCC categories with MU contents at the second milk recording was inconclusive. We concluded that CSCC categories may be a useful tool for identifying success and problems regarding the udder health of primiparous cows in early lactation.

“植物细胞工程”内容梳理及教学设计探索——基于生活实例与领域科研前沿成果

植物细胞工程是植物生物技术领域中应用最广泛、取得多项里程碑式成果的一门技术。根据多年的教学实践,提出了一些教学体验。课程内容建设上,围绕“一个总则、两个方面”展开,给学生总结了一个线索图,使他们更好地理解章节之间的逻辑关系。讲课方式上,根据该课程的特点本着“生活中的植物细胞工程、科研中的植物细胞工程”原则进行讲述,既让学生感到植物细胞的成果就在身边,又通过生物技术领域的最新研究成果,开阔学生的视野,加深对理论的理解,激发学生的学习兴趣。

补肺一号方联合呬字诀呼吸操治疗COPD患者的临床研究

目的探讨补肺一号方联合呬字诀呼吸操对慢性阻塞性肺疾病(COPD)患者的临床疗效。方法选取2022年2月至2023年2月福建中医药大学附属第二人民医院收治的COPD稳定期肺气虚证患者90例作为研究对象。按照“就诊时间顺序编号”将对象分为三组,每组30例,试验1组(补肺1号方汤剂治疗),试验2组(补肺1号方汤剂联合呬字诀操锻炼治疗),对照组(吸入硫酸沙丁胺醇气雾剂治疗)。对比外周血Th17、Treg细胞含量、慢性阻塞性肺疾病评估测试评分(CAT)、肺活量(VC)、呼气峰流速(PEF)以及第1秒用力呼气容积(FEV_(1))。结果治疗后,试验2组的外周血Th17细胞含量以及CAT评分低于试验1组与对照组,差异有统计学意义(P<0.05);试验2组比对照组、试验1组的VC、PEF、FEV_(1)数值以及外周血Treg细胞含量更高(P<0.05);试验1组的外周血Th17细胞含量以及CAT评分低于对照组,差异有统计学意义(P<0.05);对照组相比于试验1组,其VC、PEF、FEV_(1)数值以及外周血Treg细胞含量更低(P<0.05)。结论在COPD稳定期患者的治疗中,采用补肺一号方联合呬字诀呼吸操能够有效调节患者肺功能指标,改善外周血Th17、Treg细胞含量,同时改善患者咳嗽、气喘等症状,提升睡眠质量,调整患者精神状态,促进患者康复。

温湿度变化对车用燃料电池输出性能的影响

研究温湿度变化对车用燃料电池输出性能(输出电压和功率)的影响可为高精度进气控制策略提供有效的依据。本工作提出了一个温湿度-电流(temperature and relative humidity-current,TRH-C)模型,该模型考虑了电池内部电化学反应、电渗迁移和加湿冷凝三部分水来源,揭示了电流随温湿度变化规律和由水活度表征的电渗迁移系数计算式。根据电池流道实物在计算软件COMSOL中建立网格,将TRH-C模型导入并应用有限体积法进行计算;搭建了燃料电池测试系统,在工作温度60℃和70℃、相对湿度分别为50%和100%条件下进行了实验并进行数据处理;并对通过TRH-C模型得到的极化曲线与实验数据进行比较,分析了电流密度和膜水含量分布云图。结果表明,TRH-C模型能预测燃料电池的性能,在工作温度为60℃、相对湿度为50%时,电压和功率密度的相对误差最大(电流密度为0.018 A/cm^(2)),分别为3.674%和3.696%。工作温度升高会导致膜水含量降低,但相对湿度增大会导致膜水含量升高。

不同感染方式对狂犬病病毒在人二倍体细胞中增殖的影响

目的考察带毒传代感染、直接吸附感染、混种传代感染三种方式感染人二倍体细胞对狂犬病病毒增殖的影响。方法不同方式感染后,经过细胞工厂生产,获取狂犬病病毒收获液,经过滤澄清、浓缩、纯化、灭活制备疫苗原液。根据《中华人民共和国药典》(2020版)检测方法检测收获液病毒滴度及原液抗原含量。结果带毒传代组的收获液病毒滴度为(6.40±0.33)lgLD50·mL^(-1),原液抗原含量为(34.8±1.5)IU·mL^(-1);混种传代感染组的收获液病毒滴度为(6.35±0.18)lgLD50·mL^(-1),原液抗原含量为(34.3±2.1)IU·mL^(-1);直接吸附感染组收获液病毒滴度为(4.71±0.39)lgLD50·mL^(-1),原液抗原含量为(12.6±2.0)IU·mL^(-1)。结论试验证明狂犬病病毒PV株采用混种传代感染的方式感染人二倍体细胞MRC-5细胞,病毒增殖效果良好,毒种代次明确,工艺操作简单,更适合于狂犬疫苗的生产。

脱落细胞DNA定量分析技术动态监测口腔扁平苔藓伴上皮异常增生病情及光动力治疗1例

口腔扁平苔藓是一种临床上常见的慢性炎性口腔黏膜病,具有癌变风险,临床上应积极干预并随访监测。光动力治疗具有微创、有效、选择性好以及可重复性高等优势,在治疗口腔扁平苔藓中已显现出良好疗效,但其疗效评估方法仍然存在局限性。脱落细胞DNA定量分析技术是一种无创辅助检查手段,可用于口腔潜在恶性疾患癌变及口腔癌的早筛早诊。本文报道1例采用脱落细胞DNA定量分析技术动态监测口腔扁平苔藓癌变风险及光动力治疗疗效的诊疗过程,以期为建立口腔扁平苔藓伴上皮异常增生的精准微创诊疗体系提供参考。

无水氟化氢水含量的检测分析

无水氟化氢(AHF)中含有微量的水分,根据国家标准GB/T 7746—2011《工业无水氟化氢》的要求,Ⅰ类产品中的水含量要求小于50×10^(-6),精度要求较高。在生产过程中对无水氟化氢中水含量的控制有非常严格的要求,因为无水氟化氢中的水含量波动会影响产品质量同时会加剧对设备的腐蚀,因而必须及时、稳定地测定无水氟化氢中的水含量以便于生产控制。国家标准GB/T 7746—2011《工业无水氟化氢》给定了水含量的检测方法,在此基础上,经过试验分析对比,优化改进分析检测方法,完成无水氟化氢中水含量的在线检测。经现场检测验证,该方法的检测数据准确,操作简单、安全。

不同防护措施对早熟朱柿抽条的防护效果及其生理机制

选取定植1 a的早熟朱柿幼树为研究对象,设置树干缠防寒布+反光膜、树干培土、树干涂白、树干缠防寒布、枝条喷涂水果保鲜剂、枝条喷涂高脂膜、枝条喷涂石蜡乳化剂共7个防抽条处理,测定不同处理的抽条率以及一年生枝的水分状况、细胞活力和非结构性碳水化合物(NSC)含量。结果表明:防寒布+反光膜处理和培土处理抽条率为0,水果保鲜剂、高脂膜、石蜡乳化剂处理抽条率介于30%~60%之间,涂白、防寒布处理及对照的抽条率为100%。石蜡乳化剂、防寒布+反光膜处理和培土处理的凌晨水势显著高于水果保鲜剂处理和高脂膜处理(P<0.05),两组差值为0.5 MPa左右。培土处理的枝条导水损失率(PLC)较水果保鲜剂处理和高脂膜处理显著降低,分别降低10.95%和11.24%(P<0.05);韧皮部细胞活力显著高于其他处理,增幅为13.53%~34.87%(P<0.05)。培土处理的韧皮部可溶性糖含量、淀粉含量和NSC含量显著高于其他处理,分别提高13.79%~28.80%、9.15%~39.65%和14.87%~29.61%(P<0.05),培土处理的木质部可溶性糖含量、淀粉含量和NSC含量显著高于其他处理,分别提高20.59%~35.27%、14.19%~37.90%和17.40%~37.00%(P<0.05)。相关性分析结果表明,不同防抽条技术措施下的抽条率与枝条PLC呈显著正相关关系(P<0.05),与枝条凌晨水势、木质部相对含水量、韧皮部细胞活力、木质部淀粉含量、木质部NSC含量呈显著负相关关系(P<0.05)。可见,防寒布+反光膜处理和培土处理对柿树冬春季抽条有较好的防护效果,能维持枝条水分状况和韧皮部细胞活力,并促进NSC向枝条运输。

ITO薄膜的制备及其光电性能研究

随着HJT太阳电池的发展,对其氧化铟锡(ITO)薄膜的研究日益增多。通过直流磁控溅射法在玻璃衬底上制备ITO薄膜,研究了氧含量、溅射功率、沉积温度及溅射气压对ITO薄膜光电性能的影响。研究结果显示:1)ITO薄膜的光电性能对氧含量较为敏感,随着氧含量增加,ITO薄膜的电阻率也随之增加,而透过率则呈先上升后下降,然后基本保持不变的趋势;2)随着沉积温度升高,ITO薄膜的透过率也随之升高,而电阻率则呈先下降后上升的趋势;3)随着溅射气压的升高,ITO薄膜的电阻率呈上升趋势,而透过率则是先降低再略微升高;4)当氧含量在1.8%~2.0%,溅射功率在3000~4000 W,沉积温度在150~190℃,溅射气压在0.5~0.7 Pa时,ITO薄膜具有较优的光电性能。因此,在合理范围内提高沉积温度,其则会具有退火作用,有助于进一步改善ITO薄膜的光电性能。

应用无标记高内涵成像分析技术检测乳酸对肿瘤细胞增殖与迁移的促进作用

目的:探究高内涵成像分析技术在无标记检测细胞增殖与迁移中的应用效果。方法:体外培养小鼠黑色素瘤B16-F10细胞,实验组分别添加2.5、5、10、15、30 mmol/L不同浓度的乳酸,对照组加入磷酸缓冲盐溶液(PBS),使用高内涵成像分析系统每15分钟拍摄1次、连续拍摄36 h,检测不同浓度的乳酸对B16-F10细胞的增殖与迁移的影响。结果:高内涵成像分析技术检测显示,不同浓度的乳酸处理后,实验组细胞增殖速度明显快于对照组,且添加15、30 mmol/L乳酸的实验组细胞密度明显高于其他各组。使用高内涵成像分析系统软件进行细胞计数显示,处理后12 h,15、30 mmol/L乳酸添加实验组细胞数较对照组显著增加;且随着培养时间延长,15、30 mmol/L乳酸添加实验组的细胞数均显著高于对照组(均P<0.05)。高内涵成像分析系统软件统计各组处理后0~6 h细胞迁移距离,显示各实验组细胞平均迁移距离均显著高于对照组(均P<0.05),其中5 mmol/L乳酸添加实验组的细胞平均迁移距离最大;各实验组中出现迁移行为的细胞比例均显著高于对照组,随着乳酸浓度增加,迁移行为细胞比例出现降低的趋势。结论:成功建立了应用高内涵成像分析技术快速、无标记、可靠且准确的定量评价细胞增殖和迁移的方法,实验操作较为简单,且能获取直观的图像及多样化的数据统计结果。

红细胞平均体积、红细胞平均血红蛋白含量、血清铁蛋白检测对孕产妇缺铁性贫血的临床分析

目的探究红细胞平均体积(MCV)、红细胞平均血红蛋白含量(MCH)、血清铁蛋白(SF)检测对孕产妇缺铁性贫血的临床价值。方法选取56例缺铁性贫血孕产妇为探究组;另选取同期本院产检健康的56例孕产妇为对照组。两组均进行血液检查,比较MCV、MCH、SF水平,分析各指标的诊断价值。结果探究组MCV、MCH、SF水平均显著低于对照组(P<0.05)。MCV、MCH之间疾病检查率、漏诊率比较差异无统计学意义(P>0.05),SF疾病检出率较MCV、MCH更高,漏检率较MCV、MCH更低(P<0.05)。结论孕产妇出现缺铁性贫血后,MCV、MCH、SF水平均会出现不同程度的降低,上述指标可作为孕产妇缺铁性贫血诊断的重要依据,以保证孕产妇缺铁性贫血早发现、早干预,确保孕产妇健康。

Estimating biomass of phytoplankton in the Jiaozhou Bay I. Phytoplankton biomass estimated from cell volume and plasma volume

Based on the data of the Jiaozhou Bay Ecosystem Dynamic Research, cell volume and sur face area of 87 common phytoplankton species in China sea waters were calculated with assignment of the similar geometric form. The cell plasma volume, live weight, Carbon content and nitrogen content were also calculated with the methods of Mullin et al. (1966), Strathmann (1967), Eppley et al. (1970), and Taguchi (1976). After comparing these methods, we chase the method of Eppley et al. (1970) as the hot method for calculating phytoplankton carbon content in China sea waters.