Advances in sensor developments for cell culture monitoring

Cell culture encompasses procedures for extracting cells from their natural tissue and cultivating them under controlled artificial conditions. During this process, various factors, including cell physiological/morphological properties, culture environments, metabolites, and contaminants, have to be precisely controlled and monitored for the survival of cells and the pursuit of the desired properties of the cells. This review summarizes recent advances in sensor technologies and manufacturing strategies for various cell culture platforms using traditional plastics, microfluidic chips, and scalable bioreactors. We share the details of newly developed biological sensors, chemical sensors, optical sensors, electronic chip technologies, and material integration methods. The precise control of parameters based on the feedback by these sensors and electronics enhances cell culture quality and throughput.

自愈合导电复合水凝胶用于监测人体运动和3D细胞培养

导电水凝胶已在多个领域崭露头角,因为它具备将外界压力和形变转化为电信号的能力,可实现对物理和化学刺激的灵敏感知.然而,在导电水凝胶的研发中,需要克服平衡导电性和机械强度的挑战.本项工作中,为了提高导电水凝胶的力学性能,我们采用了多重策略.首先,引入了木质素-丹宁酸(lignin-TA)颗粒作为物理交联剂,同时充分利用羟丙基纤维素分子的多羟基结构,促进水凝胶半互穿聚合物网络的形成.随后,通过利用Fe^(3+)离子和TA分子之间的静电力和金属配位相互作用,进一步增强了聚合物网络之间的键合强度,提高了水凝胶的导电性.最终,所制备的导电水凝胶以其独特的网络结构,成功融合了物理和化学键的特性,并展现出卓越的自粘附、自愈合能力、电机械性能和生物相容性.这使其成为一个可用于监测人体活动和细胞增殖的理想材料.