Capture of cervical exfoliative cells on a glass slide coated by 3-glycidyloxypropyl trimethoxysilane and poly-L-lysine

A new modification method for glass slides was developed and applied to make ThinPrep Pap smears,in order to increase the adhesion ability of cervical exfoliative cells.3-glycidyloxypropyl trimethoxysilane(GOPS) was coated on the glass slides firstly on the slides,then poly-L-lysine(PLL) was covalently modified onto the above epoxy-terminated slides to form GOPS-PLL double decorated slides.The modified slides were characterized using X-ray photoelectron spectroscopy(XPS) and atomic force microscopy(AFM).The cell adhesion ability effect was tested and compared with traditional PLL coated slides by fixing the cervical exfoliative cells on the double adorned slides.The control test was conducted by the bare glass slides unmodified.The cell morphology of cervical exfoliative cells adhered on different slides was observed under the microscope after Papanicolaou staining.The number of cervical exfoliative cells on the unmodified slides,PLL coated slides and GOPS-PLL coated slides was 10307300,32837226 and 41197280(n=12),respectively.The data among the three different modification methods showed significant differences(one-way analysis of variance,ANOVA test,Po0.05).The cell capturing effect of the GOPS-PLL slide was the best among the three different modified slides.In addition,the GOPS-PLL slide could enhance the uniformity of the adhered cells and be widely applied to the ThinPrep system for cervical carcinoma screening to increase the accuracy rate of diagnosis.

贴壁细胞在载玻片爬片的新方法

目的:对传统细胞爬片方法进行改良。方法:利用1ml蓝枪头和载玻片制作,在载玻片上种植细胞制成细胞爬片,进行后续染色。结果:细胞直接爬于载玻片上,未发现细胞污染和增殖受限。与传统爬片相比细胞脱落少。结论:与传统爬片染色方法相比具有用材简单、价格低廉、爬片效果好、操作方便等优点。

不同材质微芯片内细胞生长状态分析

微系统技术在细胞生物学方面的研究中已得到广泛应用。了解细胞在微系统芯片内的生长状态,对于利用微系统技术进行细胞研究有重要的指导意义。玻璃和聚二甲基硅氧烷(polydimethylsiloxane,PDMS)是目前制作细胞培养微芯片的主要材料。通过向以二者为基底材料制作的细胞培养微芯片内导入内皮细胞进行培养,利用实验室构建的细胞成像分析系统观察和分析细胞在不同基底材料的芯片内5天的增殖情况,同时研究了基底材料的预处理方法以及培养基对细胞增殖的影响。