Invariance and Noises of Shannon Entropy for Information on Oxidative Activity of DNA in All Living Cells for Medical Diagnostics

We analyze oxidative activity of DNA due to fluorescence of chromosomes inside cells, using flow cytometry method with nanometer spatial resolution. Statistics of fluorescence is presented in histogram as frequency distributions of flashes in the dependence on their intensity and in distributions of Shannon entropy, which was defined on the base of normalized distribution of information in original histogram for frequency of flashes. We show that overall sum of entropy, i.e. total entropy E , for any histogram is invariant and has identical trends of changes all values of E(r) = lnr at reduction of histogram’ rank r. This invariance reflects informational homeostasis of chromosomes activity in multi-scale networks of entropy inside all cells in various samples of blood for DNA inside neutrophils, lymphocytes, inside all leukocytes of human and inside chicken erythrocytes for various dyes, colors and various excitations of fluorescence. Informational homeostasis of oxidative activity of 3D DNA in the full set of chromosomes inside living cells exists for any Shannon-Weaver index of biodiversity of cells, at any state of health different beings. Regulation perturbations in information activity DNA provides informational adaptability and vitality of cells at homeostasis support. Noises of entropy, during regulation of informational homeostasis, depend on the states of health in real time. The main structural reconstructions of chromosomal correlations, corresponding to self-regulation of homeostasis, occur in the most large-scale networks of entropy, for rank r<32. We show that stability of homeostasis is supported by activity of all 46 chromosomes inside cells. Patterns, hidden switching and branching in sequences of averages of H?lder and central moments for noises in regulation of homeostasis define new opportunities in diagnostics of health and immunity. All people and all aerobic beings have one overall homeostatic level for countdown of information activity of DNA inside cells. We noted very bad and dangerous properties of artificial cells with other levels of informational homeostasis for all aerobic beings in foods, medical treatment and in biotechnologies.

Study on Plant Radiation Signal Transduction for Human Self-Healing

We selected 450 patients with chronic and difficult diseases as receptors, and selected edible and medicinal plant combinations as biological signal donors, and randomly participated in a new experiment. After adjusting the patient’s biological field, the compensating bio information energy (CBE) high-tech is applied to transmit the signal of plant radiation to the patient’s body. The physical therapy of this health care mode has the characteristics of no contact, no drugs, no intervention, no toxic side effects, no pain, and no electromagnetic radiation and so on. Before and after the experiment, the main function test, cell function, cell biochemistry and body temperature of each patient were used for the same body control and data statistical analysis, and then the comprehensive integration method was used to evaluate the effect. We found that after 1 - 4 courses of treatment (7 - 28 dx 2h), the potential disease risk of patients was significantly reduced, the relevant medical indicators improved rapidly, and the cell function and symptoms improved simultaneously. The effective rate was up to 90%, the significant efficiency was up to 57%. This experiment shows that this new physical therapy can treat both symptoms and root causes, make chronic and difficult diseases self-healing and rehabilitation, and has no ethical problems. It also shows that information on plant health can play an important role in reversing cell aging and restoring cell function. Therefore, it opens up a new field of natural therapy, which can be called cell information therapy.

手机微信预约在高校教职工健康体检中的应用探讨

目的探讨手机微信预约在高校教职工健康体检中的应用效果。方法本研究选取南京市某高校2017—2018年参加体检的教职工为研究对象,每年各随机选取600人。2017年参加体检的教职工作为对照组,2018年参加体检的教职工作为观察组。对照组教职工采用学校统筹安排的体检方式参与体检,观察组教职工采用手机微信预约体检的方式参与体检。比较2组教职工体检预约的调整率,B超检查、采血化验时间及体检总时间及单项体检项目的参检率。结果观察组教职工体检预约的调整率为3.00%,低于对照组的18.83%,差异有统计学意义(P<0.05)。观察组教职工B超检查、采血化验时间及体检总时间分别为(31.25±3.87)min、(20.43±2.81)min及(100.89±9.80)min,均短于对照组的(73.56±7.25)min、(45.61±3.11)min及(191.5±14.70)min,差异均有统计学意义(P<0.05)。观察组教职工胸部DR、内科、外科、五官科参检率分别为85.17%、93.50%、69.50%及83.17%,均高于对照组的80.33%、66.83%、53.67%及50.83%,差异均有统计学意义(P<0.05)。结论将手机微信预约运用在教职工体检中,可降低教职工体检预约时间的调整率,缩短B超检查、采血化验时间及体检总时间,提高教职工胸部DR、内科、外科、五官科参检率。因此,高校医院应以教职工为中心开展体检工作,要大力推进"互联网+医疗"的健康管理模式,以提高教职工体检的体验感。

Stimulus discrimination via responses of retinal ganglion cells and dopamine-dependent modulation

Neighboring retinal ganglion cells（RGCs）fire with a high degree of correlation.It has been increasingly realized that visual perception of the environment relies on neuronal population activity to encode and transmit the information contained in stimuli.Understanding how neuronal population activity contributes to visual information processing is essential for understanding the mechanisms of visual coding.Here we simultaneously recorded spike discharges from groups of RGCs in bullfrog retina in response to visual patterns（checkerboard,horizontal grating,and full-field illumination）using a multi-electrode array system.To determine the role of synchronous activity mediated by gap junctions,we measured the correct classification rates of single cells＇firing patterns as well as the synchronization patterns of multiple neurons.We found that,under normal conditions,RGC population activity exhibited distinct response features with exposure to different stimulus patterns and had a higher rate of correct stimulus discrimination than the activity of single cells.Dopamine（1μmol/L）application did not significantly change the performance of single neuron activity,but enhanced the synchronization of the RGC population activity and decreased the rate of correct stimulus pattern discrimination.These findings suggest that the synchronous activity of RGCs plays an important role in the information coding of different types of visual patterns,and a dopamine-induced increase in synchronous activity weakens the population performance in pattern discrimination,indicating the potential role of the dopaminergic pathway in modulating the population coding process.

一种可实现原位监测的细胞培养信息化装置

对细胞进行连续的原位检测可以获得其生理过程更准确、具体的信息,帮助人们更全面深入地认识细胞个体差异以及细胞间相互作用等问题.为了实现对细胞进行连续原位检测,本文设计了一种小型化的可以与倒置显微镜等检测设备相结合的细胞培养装置.该信息化的装置通过单片机系统实现对装置培养环境的实时监测和反馈控制,并且将培养环境数据等重要信息在上位机实时显示并储存,同时还利用GSM模块实现了用户和培养装置的远程交互功能.文中通过对HO8910卵巢癌细胞的培养及原位观察实验验证了装置的培养效果.