A new strategy using an arnperometric biosensor with Escherichia coli （E. coli） that provides a rapid toxicity determination of chemical compounds is described. The CellSense biosensor system comprises a biological ...A new strategy using an arnperometric biosensor with Escherichia coli （E. coli） that provides a rapid toxicity determination of chemical compounds is described. The CellSense biosensor system comprises a biological component immobilized in intimate contact with a transducer which converts the biochemical signal into a quantifiable electrical signal. Toxicity assessment of heavy metals using E.coli biosensors could be finished within 30 min and the 50% effective concentrations （ECso） values of four heavy metals were determined. The results shows that inhibitory effects of four heavy metals to E.coli can be ranked in a decreasing order of Hg^2＋ 〉 Cu^2＋ 〉 Zn^2＋ 〉 Ni^2＋, which accords to the results of conventional bacterial counting method. The toxicity test of organic compounds by using CellSense biosensor was also demonstrated. The CellSense biosensor with E. coli shows a good, reproducible behavior and can be used for reproducible measurements.展开更多
In this study, an enzymatic electrode for glucose biosensing and bioanode of glucose/air biofuel cell has been fabricated by immobilizing poly (methylene green) (polyMG) for electrocatalytic NADH oxidation and NAD+-de...In this study, an enzymatic electrode for glucose biosensing and bioanode of glucose/air biofuel cell has been fabricated by immobilizing poly (methylene green) (polyMG) for electrocatalytic NADH oxidation and NAD+-dependent glucose dehydrogenase (GDH) for oxidizing glucose on carbon nanodots (CNDs). The polyMG-CNDscomposites obtained by electro-polymerization of dye MG molecules adsorbed on CNDs display excellent electrocatalytic activity toward NADH electro-oxidation at a low overpotential of ca. -0.10 V (vs. Ag/AgCl) and the integrated enzymatic electrode shows fast response to glucose electrooxidation. Using the fabricated GDH-based enzymatic electrode, a glucose biosensor was constructed and exhibits a wide linear dynamic range from 0 to 8 mM, a low detection limit of 0.02 μM (S/N = 3), and fast response time (ca. 4 s) under the optimized conditions. The developed glucose biosensor was used to detect glucose content in human blood with satisfactory results. The fabricated GDH-based enzymatic electrode was also employed as bioanode to assembly a glucose/air biofuel cell with the laccase-CNDs/GC as the biocathode. The maximum power density delivered by the assembled glucose/air biofuel cell reaches 3.1 μW·cm-2 at a cell voltage of 0.22 V in real sample fruit juice. The present study demonstrates that potential applications of GDH-based CNDs electrode in analytical and biomedical measurements.展开更多
Cellular metabolism arouses the changes of substance in extracellular physiological microenvironment,and the metabolic level reflects the physiological state of cells.This paper developed a novel microphysiometer auto...Cellular metabolism arouses the changes of substance in extracellular physiological microenvironment,and the metabolic level reflects the physiological state of cells.This paper developed a novel microphysiometer automatic analysis instrument based on multiparameter cell-based biosensors for quick drug analysis.This study included the multiparameter cell-based biosensors,cell culture chamber,drug auto-injection detection and analysis.The analysis instrument was capable of real-time detection for the acidic product and other chemical parameters generated by the cellular metabolism in the micro-volume.Finally,the paper employs human breast cancer cell line MCF-7 and drug experiments to verify the performance of microphysiometer,and study effects of different drugs on cell metabolism.Further,the research explores drug analysis method of the multiparameter microphysiometer.The results showed that the cell-based microphysiometer system provides a utility platform for rapid,long-term and automatic cell physiological environment detection and drug analysis.展开更多
This paper reviews a novel cell-based biosensor and Bio-MEMS which incorporate living cells as sensing elements that convert a change in immediate environment to signals conducive for processing.It is characterized wi...This paper reviews a novel cell-based biosensor and Bio-MEMS which incorporate living cells as sensing elements that convert a change in immediate environment to signals conducive for processing.It is characterized with high sensitivity,excellent selectivity and fast response and have been implemented for a number of applications ranging from pharmaceutical screening to environmental pollutant detection.This paper also introduces our recent work about Light-Addressable Potentiometric Sensors (LAPS),Field Effect Transistor (FET),Micro-Electrode Array Sensors (MEAS) and Bio-MEMS for detecting the changes of concentration of extracellular ions and the action potential of living cell under effect of drugs and environmental parameters.Finely, the paper gives some prospects of cell-based biosensors in the future.展开更多
The light-addressable potentiometric sensor (LAPS) is a semiconductor-based cellular biosensor with an electrolyte-insulator-semiconductor (EIS) structure.By depositing biocompatible layers on the sensing surface fo...The light-addressable potentiometric sensor (LAPS) is a semiconductor-based cellular biosensor with an electrolyte-insulator-semiconductor (EIS) structure.By depositing biocompatible layers on the sensing surface for cell culture, it can be used to detect bioelectrical parameters of cells.The characteristic curve for photocurrent versus applied bias voltage to the system shows a current-voltage curve (Ⅰ-Ⅴcurve).This technique can be used to detect the action potential changes towards different drugs based on the bias voltage dependence of an optical current,and provides a dynamic system by scanning light beam at the very cell on the sensor device.The LAPS overcomes the limitation of recording sites,but high spatial resolution and sensitivity are also paramount.This paper discussed a novel structure of LAPS array for extracellular monitoring to decrease potential noise level.Both characteristics of active recording array areas and cell culture conditions are measured.展开更多
Copper Zinc Antimony Sulfide(CZAS)is derived from Copper Antimony Sulfide(CAS),a famatinite class of compound.In the current paper,the first step for using Copper,Zinc,Antimony and Sulfide as materials in manufacturin...Copper Zinc Antimony Sulfide(CZAS)is derived from Copper Antimony Sulfide(CAS),a famatinite class of compound.In the current paper,the first step for using Copper,Zinc,Antimony and Sulfide as materials in manufacturing synchrotronic biosensor-namely increasing the sensitivity of biosensor through creating Copper Zinc Antimony Sulfide,CZAS(Cu1.18Zn0.40Sb1.90S7.2)semiconductor and using it instead of Copper Tin Sulfide,CTS(Cu2SnS3)for tracking,monitoring,imaging,measuring,diagnosing and detecting cancer cells,is evaluated.Further,optimization of tris(2,2'-bipyridyl)ruthenium(II)(Ru(bpy)32+)concentrations and Copper Zinc Antimony Sulfide,CZAS(Cu1.18Zn0.40Sb1.90S7.2)semiconductor as two main and effective materials in the intensity of synchrotron for tracking,monitoring,imaging,measuring,diagnosing and detecting cancer cells are considered so that the highest sensitivity obtains.In this regard,various concentrations of two materials were prepared and photon emission was investigated in the absence of cancer cells.On the other hand,ccancer diagnosis requires the analysis of images and attributes as well as collecting many clinical and mammography variables.In diagnosis of cancer,it is important to determine whether a tumor is benign or malignant.The information about cancer risk prediction along with the type of tumor are crucial for patients and effective medical decision making.An ideal diagnostic system could effectively distinguish between benign and malignant cells;however,such a system has not been created yet.In this study,a model is developed to improve the prediction probability of cancer.It is necessary to have such a prediction model as the survival probability of cancer is high when patients are diagnosed at early stages.展开更多
This paper presents a novel biosensor for bitter substance detection on the basis of light addressable potentiometric sensor(LAPS).Taste receptor cells(TRCs)were used as sensitive elements,which can respond to differe...This paper presents a novel biosensor for bitter substance detection on the basis of light addressable potentiometric sensor(LAPS).Taste receptor cells(TRCs)were used as sensitive elements,which can respond to different bitter stimuli with extreme high sensitivity and speci-ficity.TRCs were isolated from the taste buds of rats and cultured on the surface of LAPS chip.Due to the unique advantages such as single-cell recording,light addressable capability,and noninvasiveness,LAPS chip was used as secondary transducer to monitor the responses of TRCs by recording extracelluar potential changes.The results indicate LAPS chip can effectively record the responses of TRCs to different bitter substances used in this study in a real-time manner for a long-term.In addition,by performing principal component analysis on the LAPS recording data,different bitter substances tested can be successfully discriminated.It is suggested this TRCsLAPS hybrid biosensor could be a valuable tool for bitter substance detection.With further improvement and novel design,it has great potentials to be applied in both basic research and practical applications related to bitter taste detection.展开更多
Developing flexible bioelectronics is essential to the realization of artificial intelligence devices and biomedical applications, such as wearables, but their potential is limited by sustainable energy supply. An enz...Developing flexible bioelectronics is essential to the realization of artificial intelligence devices and biomedical applications, such as wearables, but their potential is limited by sustainable energy supply. An enzymatic biofuel cell(BFC) is promising for power supply, but its use is limited by the challenges of incorporating multiple enzymes and rigid platforms. This paper shows the first example of screen-printable nanocomposite inks engineered for a single-enzyme-based energy-harvesting device and a self-powered biosensor driven by glucose on bioanode and biocathode. The anode ink is modified with naphthoquinone and multiwalled carbon nanotubes(MWCNTs), whereas the cathode ink is modified with Prussian blue/MWCNT hybrid before immobilizing with glucose oxidase. The flexible bioanode and the biocathode consume glucose. This BFC yields an open circuit voltage of 0.45 V and a maximum power density of 266 μW cm-2. The wearable device coupled with a wireless portable system can convert chemical energy into electric energy and detect glucose in artificial sweat. The self-powered sensor can detect glucose concentrations up to 10 mM. Common interfering substances,including lactate, uric acid, ascorbic acid, and creatinine, have no effect on this self-powered biosensor. Additionally, the device can endure multiple mechanical deformations. New advances in ink development and flexible platforms enable a wide range of applications, including on-body electronics, self-sustainable applications, and smart fabrics.展开更多
基金supported by the National Natural Science Foundation of China(No.20707014)the Program for Young Excellent Talents of Tongji University.
文摘A new strategy using an arnperometric biosensor with Escherichia coli （E. coli） that provides a rapid toxicity determination of chemical compounds is described. The CellSense biosensor system comprises a biological component immobilized in intimate contact with a transducer which converts the biochemical signal into a quantifiable electrical signal. Toxicity assessment of heavy metals using E.coli biosensors could be finished within 30 min and the 50% effective concentrations （ECso） values of four heavy metals were determined. The results shows that inhibitory effects of four heavy metals to E.coli can be ranked in a decreasing order of Hg^2＋ 〉 Cu^2＋ 〉 Zn^2＋ 〉 Ni^2＋, which accords to the results of conventional bacterial counting method. The toxicity test of organic compounds by using CellSense biosensor was also demonstrated. The CellSense biosensor with E. coli shows a good, reproducible behavior and can be used for reproducible measurements.
文摘In this study, an enzymatic electrode for glucose biosensing and bioanode of glucose/air biofuel cell has been fabricated by immobilizing poly (methylene green) (polyMG) for electrocatalytic NADH oxidation and NAD+-dependent glucose dehydrogenase (GDH) for oxidizing glucose on carbon nanodots (CNDs). The polyMG-CNDscomposites obtained by electro-polymerization of dye MG molecules adsorbed on CNDs display excellent electrocatalytic activity toward NADH electro-oxidation at a low overpotential of ca. -0.10 V (vs. Ag/AgCl) and the integrated enzymatic electrode shows fast response to glucose electrooxidation. Using the fabricated GDH-based enzymatic electrode, a glucose biosensor was constructed and exhibits a wide linear dynamic range from 0 to 8 mM, a low detection limit of 0.02 μM (S/N = 3), and fast response time (ca. 4 s) under the optimized conditions. The developed glucose biosensor was used to detect glucose content in human blood with satisfactory results. The fabricated GDH-based enzymatic electrode was also employed as bioanode to assembly a glucose/air biofuel cell with the laccase-CNDs/GC as the biocathode. The maximum power density delivered by the assembled glucose/air biofuel cell reaches 3.1 μW·cm-2 at a cell voltage of 0.22 V in real sample fruit juice. The present study demonstrates that potential applications of GDH-based CNDs electrode in analytical and biomedical measurements.
基金This work was supported by the National Natural Science Foundation of China(Grant No.81027003)the Zhejiang Provincial Science and Technology Major Special Program(No.2010C14006).
文摘Cellular metabolism arouses the changes of substance in extracellular physiological microenvironment,and the metabolic level reflects the physiological state of cells.This paper developed a novel microphysiometer automatic analysis instrument based on multiparameter cell-based biosensors for quick drug analysis.This study included the multiparameter cell-based biosensors,cell culture chamber,drug auto-injection detection and analysis.The analysis instrument was capable of real-time detection for the acidic product and other chemical parameters generated by the cellular metabolism in the micro-volume.Finally,the paper employs human breast cancer cell line MCF-7 and drug experiments to verify the performance of microphysiometer,and study effects of different drugs on cell metabolism.Further,the research explores drug analysis method of the multiparameter microphysiometer.The results showed that the cell-based microphysiometer system provides a utility platform for rapid,long-term and automatic cell physiological environment detection and drug analysis.
基金Acknowledgement： This work was supported by the National Natural Science Foundation of China （Grant Nos. 30270387, No. 30570492）；the Project of State Key Laboratory of Transducer Technology of China （Grant No. SKT0403）；the Foundation for the Bureau of Zhejiang Province of China （Grant No. 20040197）.
文摘This paper reviews a novel cell-based biosensor and Bio-MEMS which incorporate living cells as sensing elements that convert a change in immediate environment to signals conducive for processing.It is characterized with high sensitivity,excellent selectivity and fast response and have been implemented for a number of applications ranging from pharmaceutical screening to environmental pollutant detection.This paper also introduces our recent work about Light-Addressable Potentiometric Sensors (LAPS),Field Effect Transistor (FET),Micro-Electrode Array Sensors (MEAS) and Bio-MEMS for detecting the changes of concentration of extracellular ions and the action potential of living cell under effect of drugs and environmental parameters.Finely, the paper gives some prospects of cell-based biosensors in the future.
基金This work was supported by the National Natural Science Foundation of China （Grant Nos. 30270387, No. 30570492）；the Project of State Key Laboratory of Transducer Technology of China （Grant No. SKT0403）；the Foundation for the Bureau of Zhejiang Province of China （Grant No. 20040197）.
文摘The light-addressable potentiometric sensor (LAPS) is a semiconductor-based cellular biosensor with an electrolyte-insulator-semiconductor (EIS) structure.By depositing biocompatible layers on the sensing surface for cell culture, it can be used to detect bioelectrical parameters of cells.The characteristic curve for photocurrent versus applied bias voltage to the system shows a current-voltage curve (Ⅰ-Ⅴcurve).This technique can be used to detect the action potential changes towards different drugs based on the bias voltage dependence of an optical current,and provides a dynamic system by scanning light beam at the very cell on the sensor device.The LAPS overcomes the limitation of recording sites,but high spatial resolution and sensitivity are also paramount.This paper discussed a novel structure of LAPS array for extracellular monitoring to decrease potential noise level.Both characteristics of active recording array areas and cell culture conditions are measured.
文摘Copper Zinc Antimony Sulfide(CZAS)is derived from Copper Antimony Sulfide(CAS),a famatinite class of compound.In the current paper,the first step for using Copper,Zinc,Antimony and Sulfide as materials in manufacturing synchrotronic biosensor-namely increasing the sensitivity of biosensor through creating Copper Zinc Antimony Sulfide,CZAS(Cu1.18Zn0.40Sb1.90S7.2)semiconductor and using it instead of Copper Tin Sulfide,CTS(Cu2SnS3)for tracking,monitoring,imaging,measuring,diagnosing and detecting cancer cells,is evaluated.Further,optimization of tris(2,2'-bipyridyl)ruthenium(II)(Ru(bpy)32+)concentrations and Copper Zinc Antimony Sulfide,CZAS(Cu1.18Zn0.40Sb1.90S7.2)semiconductor as two main and effective materials in the intensity of synchrotron for tracking,monitoring,imaging,measuring,diagnosing and detecting cancer cells are considered so that the highest sensitivity obtains.In this regard,various concentrations of two materials were prepared and photon emission was investigated in the absence of cancer cells.On the other hand,ccancer diagnosis requires the analysis of images and attributes as well as collecting many clinical and mammography variables.In diagnosis of cancer,it is important to determine whether a tumor is benign or malignant.The information about cancer risk prediction along with the type of tumor are crucial for patients and effective medical decision making.An ideal diagnostic system could effectively distinguish between benign and malignant cells;however,such a system has not been created yet.In this study,a model is developed to improve the prediction probability of cancer.It is necessary to have such a prediction model as the survival probability of cancer is high when patients are diagnosed at early stages.
基金This work was supported by the grants from the National Natural Science Foundation of China(Grant Nos.60725102,31000448)the China Postdoctoral Science Foundation(Grant Nos.20100471737,201104734)the Key Project of Zhejiang Province(Grant No.2010C14006).
文摘This paper presents a novel biosensor for bitter substance detection on the basis of light addressable potentiometric sensor(LAPS).Taste receptor cells(TRCs)were used as sensitive elements,which can respond to different bitter stimuli with extreme high sensitivity and speci-ficity.TRCs were isolated from the taste buds of rats and cultured on the surface of LAPS chip.Due to the unique advantages such as single-cell recording,light addressable capability,and noninvasiveness,LAPS chip was used as secondary transducer to monitor the responses of TRCs by recording extracelluar potential changes.The results indicate LAPS chip can effectively record the responses of TRCs to different bitter substances used in this study in a real-time manner for a long-term.In addition,by performing principal component analysis on the LAPS recording data,different bitter substances tested can be successfully discriminated.It is suggested this TRCsLAPS hybrid biosensor could be a valuable tool for bitter substance detection.With further improvement and novel design,it has great potentials to be applied in both basic research and practical applications related to bitter taste detection.
基金supported by National Research Council of Thailand NRCT (grant number: N41A640129), Prince of Songkla University, Hat Yai, Thailandthe Talent Management Project of Prince of Songkla Universitythe Center of Excellence for Innovation in Chemistry (PERCH-CIC), Ministry of Higher Education, Science, Research, and Innovation (MHESI)。
文摘Developing flexible bioelectronics is essential to the realization of artificial intelligence devices and biomedical applications, such as wearables, but their potential is limited by sustainable energy supply. An enzymatic biofuel cell(BFC) is promising for power supply, but its use is limited by the challenges of incorporating multiple enzymes and rigid platforms. This paper shows the first example of screen-printable nanocomposite inks engineered for a single-enzyme-based energy-harvesting device and a self-powered biosensor driven by glucose on bioanode and biocathode. The anode ink is modified with naphthoquinone and multiwalled carbon nanotubes(MWCNTs), whereas the cathode ink is modified with Prussian blue/MWCNT hybrid before immobilizing with glucose oxidase. The flexible bioanode and the biocathode consume glucose. This BFC yields an open circuit voltage of 0.45 V and a maximum power density of 266 μW cm-2. The wearable device coupled with a wireless portable system can convert chemical energy into electric energy and detect glucose in artificial sweat. The self-powered sensor can detect glucose concentrations up to 10 mM. Common interfering substances,including lactate, uric acid, ascorbic acid, and creatinine, have no effect on this self-powered biosensor. Additionally, the device can endure multiple mechanical deformations. New advances in ink development and flexible platforms enable a wide range of applications, including on-body electronics, self-sustainable applications, and smart fabrics.
