Phosphorylation of β-catenin tyrosine residue 654 plays an important role in the epithelial to myofibroblast transition （EMT）. Introducing mimic peptide of tyrosine residue 654 domain of β-catenin into cells may i...Phosphorylation of β-catenin tyrosine residue 654 plays an important role in the epithelial to myofibroblast transition （EMT）. Introducing mimic peptide of tyrosine residue 654 domain of β-catenin into cells may influence phosphorylation of β-catenin tyrosine residue 654. To deliver this mimic peptide into renal epithelial cells, we used penetratin as a vector, which is a novel cell permeable peptide, to deliver hydrophilic molecules into cells. A tyrosine 654 residue domain mimic peptide of β-catenin （PM） with fused penetratin was constructed, purified and then detected for the penetration of the mimic peptide into human renal tubular epithelial cells （HK-2）. The results showed that purified fusion mimic peptide could efficiently and rapidly translocate into human renal tubular epithelial cells. It is concluded that a cell-permeable peptides mimic of tyrosine residue 654 domain of β-catenin was successfully obtained, which may provide a useful reagent for interfering the human renal tubular epithelial-mesenchymal transition.展开更多
基金the National Natural Sciences Foundation of China (No. 30370657).
文摘Phosphorylation of β-catenin tyrosine residue 654 plays an important role in the epithelial to myofibroblast transition （EMT）. Introducing mimic peptide of tyrosine residue 654 domain of β-catenin into cells may influence phosphorylation of β-catenin tyrosine residue 654. To deliver this mimic peptide into renal epithelial cells, we used penetratin as a vector, which is a novel cell permeable peptide, to deliver hydrophilic molecules into cells. A tyrosine 654 residue domain mimic peptide of β-catenin （PM） with fused penetratin was constructed, purified and then detected for the penetration of the mimic peptide into human renal tubular epithelial cells （HK-2）. The results showed that purified fusion mimic peptide could efficiently and rapidly translocate into human renal tubular epithelial cells. It is concluded that a cell-permeable peptides mimic of tyrosine residue 654 domain of β-catenin was successfully obtained, which may provide a useful reagent for interfering the human renal tubular epithelial-mesenchymal transition.
