Cell sheet engineering,which fabricates sheet-like tissues without biodegradable scaffolds,has been proposed as a novel approach for tissue engineering.Cells have been cultured and proliferate to confluence on a tempe...Cell sheet engineering,which fabricates sheet-like tissues without biodegradable scaffolds,has been proposed as a novel approach for tissue engineering.Cells have been cultured and proliferate to confluence on a temperature-responsive cell culture surface at 37℃.By decreasing temperature to 20℃,an intact cell sheet can be harvested from the culture surface without enzymatic treatment.This new approach enables cells to keep their cell–cell junction,cell surface proteins and extracellular matrix.Therefore,recovered cell sheet can be easily not only transplanted to host tissue,but also constructed a three-dimensional(3D)tissue by layering cell sheets.Moreover,cell sheet manipulation technology and bioreactor have been combined with the cell sheet technology to fabricate a complex and functional 3D tissue in vitro.So far,cell sheet technology has been applied in regenerative medicine for several tissues,and a number of clinical studies have been performed.In this review,recent advances in the preparation of temperature-responsive cell culture surface,the fabrication of organ-like tissue and the clinical application of cell sheet engineering are summarized and discussed.展开更多
基金This study was partially supported by Creation of innovation centers for advanced interdisciplinary research areas Program in the Project for Developing Innovation Systems‘Cell Sheet Tissue Engineering Center(CSTEC)’from the Ministry of Education,Culture,Sports,Science and Technology(MEXT),Japan.
文摘Cell sheet engineering,which fabricates sheet-like tissues without biodegradable scaffolds,has been proposed as a novel approach for tissue engineering.Cells have been cultured and proliferate to confluence on a temperature-responsive cell culture surface at 37℃.By decreasing temperature to 20℃,an intact cell sheet can be harvested from the culture surface without enzymatic treatment.This new approach enables cells to keep their cell–cell junction,cell surface proteins and extracellular matrix.Therefore,recovered cell sheet can be easily not only transplanted to host tissue,but also constructed a three-dimensional(3D)tissue by layering cell sheets.Moreover,cell sheet manipulation technology and bioreactor have been combined with the cell sheet technology to fabricate a complex and functional 3D tissue in vitro.So far,cell sheet technology has been applied in regenerative medicine for several tissues,and a number of clinical studies have been performed.In this review,recent advances in the preparation of temperature-responsive cell culture surface,the fabrication of organ-like tissue and the clinical application of cell sheet engineering are summarized and discussed.
