With the development of biomaterials,more attention is paid to the adhesion characteristics between cells and materials.It is necessary to study the adhesive force with a suitable method.Silk fibroin(SF)is widely inve...With the development of biomaterials,more attention is paid to the adhesion characteristics between cells and materials.It is necessary to study the adhesive force with a suitable method.Silk fibroin(SF)is widely investigated in biomedical application due to its novel biocompatibility and mechanical properties.In this article,the micropipette aspiration method and measurement pattern of uniform cells in round shape(UCR)was used to study the initial adhesive force of three types of cells on pure silk fibroin films(SFFs).We also compared the adhesive forces of modified SFFs with that of pure SFFs.The results of adhesive force in the initial adhesive stage were in concordance with the results of MTT assay andmicroscope observation,which were confirmed by the above three cell lines and four kinds of SFFs.The results indicated UCR was an efficient and quantitative measurement pattern in initial adhesion stage.This article also provides a useful method in identifying initial cell-materials interactions.展开更多
基金This work was supported by the National Natural Science Foundation of China(NSFC,no.51502192,11502158,31501212 and 51503140)Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi(STIP,no.2016142)+4 种基金National Basic Research Program of China(973 project,2005CB623906)Natural Science Foundation for Young Scientists of Shanxi Province(no.2014021039-6 and 201601D021127)the Qualified Personnel Foundation of Taiyuan University of Technology(QPFT,no.tyut-rc201270a)the Youth Foundation of Taiyuan University of Technology(no.1205-04020102,2013Z020 and 2014TD066)the Technical Services Project of Taiyuan University of Technology(no.143230043-J).
文摘With the development of biomaterials,more attention is paid to the adhesion characteristics between cells and materials.It is necessary to study the adhesive force with a suitable method.Silk fibroin(SF)is widely investigated in biomedical application due to its novel biocompatibility and mechanical properties.In this article,the micropipette aspiration method and measurement pattern of uniform cells in round shape(UCR)was used to study the initial adhesive force of three types of cells on pure silk fibroin films(SFFs).We also compared the adhesive forces of modified SFFs with that of pure SFFs.The results of adhesive force in the initial adhesive stage were in concordance with the results of MTT assay andmicroscope observation,which were confirmed by the above three cell lines and four kinds of SFFs.The results indicated UCR was an efficient and quantitative measurement pattern in initial adhesion stage.This article also provides a useful method in identifying initial cell-materials interactions.
