This work focuses on the interaction between polymeric micelles with different charged surfaces and cancer cells in order to study the influence of surface charge on the in vitro cellular uptake efficiency. The amphip...This work focuses on the interaction between polymeric micelles with different charged surfaces and cancer cells in order to study the influence of surface charge on the in vitro cellular uptake efficiency. The amphiphilic diblock copolymers poly（e-caprolaetone）-b-poly（ethylene oxide） （PCL-b-PEO） with different functional groups at the end of hydrophilic block were synthesized. The functional groups endue the micelles with different charges on the surfaces. The cellular uptake of micelles to T-24 cells （human bladder tumor cells）, HepG2 cells （human liver hepatocellular carcinoma cell line） and Hela cells （human epithelial cervical cancer cells） was studied by means of flow cytometer and confbcal laser scanning microscopy. The results indicate that the surface charges showed great influence on zeta potential of micelles at different pH values. The in vitro cellular uptake efficiency of micelles with different charged surfaces demonstrated different cellular uptake patterns to three kinds of cancer cells.展开更多
Here the effect of solid retention time(SRT) on the concentration of the mixed liquor suspend solid(MLSS),the sludge characteristics,the content of extra-cellular polymeric substances(EPS),the viscosity of mixed liquo...Here the effect of solid retention time(SRT) on the concentration of the mixed liquor suspend solid(MLSS),the sludge characteristics,the content of extra-cellular polymeric substances(EPS),the viscosity of mixed liquor and effluent quality in the immersed membrane bioreactor(IMBR) was investi-gated.The results indicate that the increase of the EPS content is the main reason for the increase of mixed liquor viscosity,the former is positively correlated with the latter(R2 = 0.9751).The size distribution profile of particles in the mixed liquor presents double-peak shape at SRT more than 30 days.The filtration resistance of membrane in IMBR is mainly caused by the tiny particles and the viscosity of the mixed liquor.In this study,the extension of SRT can hardly affect the removal efficiency of Chemical Oxygen Demand(COD) and NH+4―N,and when SRT is below 30 days,silt density index(SDI15) is less than 3,the effluent can be deeply treated by using reverse osmosis system(RO) or nano-filtration system(NF).The method of controlling SRT is put forward by analyzing the relationship between SRT and the minimum generation-time of dominant bacteria(at the maximum specific growth rate under the operation temperature).展开更多
基金supported by the National Natural Science Foundation of China (Nos. 51025314, 50973122,)the Knowledge Innovation Program of the Chinese Academy of Sciences (No. KJCX2-YW-H19)
文摘This work focuses on the interaction between polymeric micelles with different charged surfaces and cancer cells in order to study the influence of surface charge on the in vitro cellular uptake efficiency. The amphiphilic diblock copolymers poly（e-caprolaetone）-b-poly（ethylene oxide） （PCL-b-PEO） with different functional groups at the end of hydrophilic block were synthesized. The functional groups endue the micelles with different charges on the surfaces. The cellular uptake of micelles to T-24 cells （human bladder tumor cells）, HepG2 cells （human liver hepatocellular carcinoma cell line） and Hela cells （human epithelial cervical cancer cells） was studied by means of flow cytometer and confbcal laser scanning microscopy. The results indicate that the surface charges showed great influence on zeta potential of micelles at different pH values. The in vitro cellular uptake efficiency of micelles with different charged surfaces demonstrated different cellular uptake patterns to three kinds of cancer cells.
基金Supported by Natural Science Foundation of Tianjin (Grant No.08JC2DJC24300)
文摘Here the effect of solid retention time(SRT) on the concentration of the mixed liquor suspend solid(MLSS),the sludge characteristics,the content of extra-cellular polymeric substances(EPS),the viscosity of mixed liquor and effluent quality in the immersed membrane bioreactor(IMBR) was investi-gated.The results indicate that the increase of the EPS content is the main reason for the increase of mixed liquor viscosity,the former is positively correlated with the latter(R2 = 0.9751).The size distribution profile of particles in the mixed liquor presents double-peak shape at SRT more than 30 days.The filtration resistance of membrane in IMBR is mainly caused by the tiny particles and the viscosity of the mixed liquor.In this study,the extension of SRT can hardly affect the removal efficiency of Chemical Oxygen Demand(COD) and NH+4―N,and when SRT is below 30 days,silt density index(SDI15) is less than 3,the effluent can be deeply treated by using reverse osmosis system(RO) or nano-filtration system(NF).The method of controlling SRT is put forward by analyzing the relationship between SRT and the minimum generation-time of dominant bacteria(at the maximum specific growth rate under the operation temperature).
