Isolation of Cordyceps ophioglossoides L2 from Fruit Body and Optimization of Fermentation Conditions for Its Mycelial Growth

A strain isolated from the fruiting body of a fungus parasitized on Elaphomyces was identified as Cordyceps ophioglossoides based on the morphological characteristics and the analysis of ITS-5.8s rDNA sequence. The optimal medium, composition （g·L^-1）, containing sucrose 66.0, yeast powder 10.0, silkworm chrysalises digest 30.0, MgSO4· 7H2O 0.4, and KH2PO4 0.4, Was found using fractional factorial design ancl a central composite design, and the optimization of cultural conditions obtained a result of seed age 6 days, inoculum size 6% （by volume）, initial pH 5.6, temperature 24℃, shaking speed 160 ·＇min^-1 by one-factor-at-a-time method. The maximum biomass reached about 20.2 g·L^-1 after 90 hours culture under the optimal conditions. Elementary nharmaeclogical actlwtties showed that mycelia of C. ophioglossoides L2 from submerged culture promoted Uterus growth in estrogen- depleted mice. In the 15-litre scale-up fermentation, the mycelial biomass was around 19.1 g·L^-1, indicating a promising prospect for this biotechnoloagy and the potency to develoo its medical value.

Six-Element Yagi Array Designs Using Central Force Optimization with Pseudo Random Negative Gravity

A six-element Yagi-Uda array is optimally designed using Central Force Optimization (CFO) with a small amount of pseudo randomly injected negative gravity. CFO is a simple, deterministic metaheuristic analogizing gravitational kinematics (motion of masses under the influence of gravity). It has been very effective in addressing a wide range of antenna and other problems and normally employs only positive gravity. With positive gravity the six element CFO-designed Yagi array described here exhibits excellent performance with respect to the objectives of impedance bandwidth and forward gain. This paper addresses the question of what happens when a small amount of negative gravity is injected into the CFO algorithm. Does doing so have any effect, beneficial, negative or neutral? In this particular case negative gravity improves CFO’s exploration and creates a region of optimality containing many designs that perform about as well as or better than the array discovered with only positive gravity. Without some negative gravity these array configurations are overlooked. This Yagi-Uda array design example suggests that antennas optimized or designed using deterministic CFO may well benefit by including a small amount of negative gravity, and that the negative gravity approach merits further study.

Central Force Optimization with Gravity <0, Elitism, and Dynamic Threshold Optimization: An Antenna Application, 6-Element Yagi-Uda Arrays

This paper investigates the effect of adding three extensions to Central Force Optimization when it is used as the Global Search and Optimization method for the design and optimization of 6-elementYagi-Uda arrays. Those extensions are Negative Gravity, Elitism, and Dynamic Threshold Optimization. The basic CFO heuristic does not include any of these, but adding them substantially improves the algorithm’s performance. This paper extends the work reported in a previous paper that considered only negative gravity and which showed a significant performance improvement over a range of optimized arrays. Still better results are obtained by adding to the mix Elitism and DTO. An overall improvement in best fitness of 19.16% is achieved by doing so. While the work reported here was limited to the design/optimization of 6- element Yagis, the reasonable inference based on these data is that any antenna design/optimization problem, indeed any Global Search and Optimization problem, antenna or not, utilizing Central Force Optimization as the Global Search and Optimization engine will benefit by including all three extensions, probably substantially.

Optimal control based coordinated taxiing path planning and tracking for multiple carrier aircraft on flight deck

Coordinated taxiing planning for multiple aircraft on flight deck is of vital importance which can dramatically improve the dispatching efficiency.In this paper,first,the coordinated taxiing path planning problem is transformed into a centralized optimal control problem where collision-free conditions and mechanical limits are considered.Since the formulated optimal control problem is of large state space and highly nonlinear,an efficient hierarchical initialization technique based on the Dubins-curve method is proposed.Then,a model predictive controller is designed to track the obtained reference trajectory in the presence of initial state error and external disturbances.Numerical experiments demonstrate that the proposed“offline planningþonline tracking”framework can achieve efficient and robust coordinated taxiing planning and tracking even in the presence of initial state error and continuous external disturbances.

Pseudomonas sp.ZXY-1,a newly isolated and highly efficient atrazine-degrading bacterium,and optimization of biodegradation using response surface methodology

Atrazine, a widely used herbicide, is increasing the agricultural production effectively, while also causing great environmental concern. Efficient atrazine-degrading bacterium is necessary to removal atrazine rapidly to keep a safe environment. In the present study, a new atrazine-degrading strain ZXY-1, identified as Pseudomonas, was isolated. This new isolated strain has a strong ability to biodegrade atrazine with a high efficiency of 9.09 mg/L/hr.Temperature, p H, inoculum size and initial atrazine concentration were examined to further optimize the degradation of atrazine, and the synthetic effect of these factors were investigated by the response surface methodology. With a high quadratic polynomial mathematical model（R^2= 0.9821） being obtained, the highest biodegradation efficiency of 19.03 mg/L/hr was reached compared to previous reports under the optimal conditions（30.71°C, pH 7.14, 4.23%（V/V） inoculum size and 157.1 mg/L initial atrazine concentration）.Overall, this study provided an efficient bacterium and approach that could be potentially useful for the bioremediation of wastewater containing atrazine.

Distributed Design of Approximately Optimal Controller for Identical Discrete-Time Multi-Agent Systems

This paper considers the distributed control of the LQR problem for discrete-time multiagent systems.Distributed controllers are designed based on the solutions of centralized optimal control and the topological structure of the systems.Under mild conditions,it is shown that the distributed controller can approximate to the centralized optimal controller.Then the states of the closed-loop systems of the distributed control exponentially converge to the states of the closed-loop systems of the centralized optimal control.Some examples are given to show effectiveness of the proposed results.