One human autoimmune serum was identified to react with centrosomes by immunofluorescence. We applied the affinity purification of membrane-bound antibody technique and demonstrated that the antibodies present in this...One human autoimmune serum was identified to react with centrosomes by immunofluorescence. We applied the affinity purification of membrane-bound antibody technique and demonstrated that the antibodies present in this antiserum reacted with a 31/29 ku centrosomal antigen. Immunofluorescence showed that this antigen is located at cen-trosome in a cell-cycle independent manner, and thereby it belongs to the family of centrosomal residents. We then utilized this autoimmune serum and antibodies against centrin and gamma-tubulin to investigate changes of centrosome cycle kinetics during premature chromosome condensation (PCC) artificially induced in V79-8 cells. We show here that centrosomal proteins continue to express when cells are syn-chronized at G1/S boundary and S phase by Hydroxyurea (HU). During this tune, the addition of caffeine causes cells with unreplicated genome to go into mitosis, and induces the separation of the replicated centrosomes. These results suggest that the coordination of DNA展开更多
基金This work was supported by the National Natural Science Foundation of China (Grant No. 39430080) the State Major Basic Research Program of China (Grant No. G1999053901) from the doctoral fund of Ministry Education of China (Grant No. 96002713).
文摘One human autoimmune serum was identified to react with centrosomes by immunofluorescence. We applied the affinity purification of membrane-bound antibody technique and demonstrated that the antibodies present in this antiserum reacted with a 31/29 ku centrosomal antigen. Immunofluorescence showed that this antigen is located at cen-trosome in a cell-cycle independent manner, and thereby it belongs to the family of centrosomal residents. We then utilized this autoimmune serum and antibodies against centrin and gamma-tubulin to investigate changes of centrosome cycle kinetics during premature chromosome condensation (PCC) artificially induced in V79-8 cells. We show here that centrosomal proteins continue to express when cells are syn-chronized at G1/S boundary and S phase by Hydroxyurea (HU). During this tune, the addition of caffeine causes cells with unreplicated genome to go into mitosis, and induces the separation of the replicated centrosomes. These results suggest that the coordination of DNA
