Identification and Fine Mapping of a Gene Related to Pale Green Leaf Phenotype near the Centromere Region in Rice(Oryza sativa)

A thermo-insensitive pale green leaf mutant （pgl2） was isolated from T-DNA inserted transgenic lines of rice （Oryza sativa L. subsp, japonica cv. Nipponbare）. Genetic analysis indicated that the phenotype was caused by a recessive mutation in a single nuclear-encoded gene. To map the PGL2gene, an F2 population was constructed by crossing the mutant with Longtefu （Oryza sativa L. subsp, indica）. The PGL2 locus was roughly linked to SSR marker RM331 on chromosome 8. To finely map the gene, 14 new InDel markers were developed around the marker, and PGL2 was further mapped to a 2.37 Mb centromeric region. Analysis on chlorophyll contents of leaves showed that there was no obvious difference between the mutant and the wild type in total chlorophyll （Chl） content, while the ratio of Chl a / Chl b in the mutant was only about 1, which was distinctly lower than that in the wild type, suggesting that the PGL2 gene was related to the conversion between Chl a and Chl b. Moreover, the method of primer design around the centromeric region was discussed, which would provide insight into fine mapping of the functional genes in plant centromeres.

Overexpression of centromere protein H is significantly associated with breast cancer progression and overall patient survival

Breast cancer is one of the leading causes of cancer death worldwide.This study aimed to analyze the expression of centromere protein H(CENP-H) in breast cancer and to correlate it with clinicopathologic data,including patient survival.Using reverse transcription-polymerase chain reaction and Western blotting to detect the expression of CENP-H in normal mammary epithelial cells,immortalized mammary epithelial cell lines,and breast cancer cell lines,we observed that the mRNA and protein levels of CENP-H were higher in breast cancer cell lines and in immortalized mammary epithelial cells than in normal mammary epithelial cells.We next examined CENP-H expression in 307 paraffin-embedded archived samples of clinicopathologically characterized breast cancer using immunohistochemistry,and detected high CENP-H expression in 134(43.6%) samples.Statistical analysis showed that CENP-H expression was related with clinical stage(P = 0.001),T classification(P = 0.032),N classification(P = 0.018),and Ki-67(P < 0.001).Patients with high CENP-H expression had short overall survival.Multivariate analysis showed that CENP-H expression was an independent prognostic indicator for patient survival.Our results suggest that CENP-H protein is a valuable marker of breast cancer progression and prognosis.

Differential Expressing of Centromere Protein CenpG in Breast Cancer

Using indirect immunofluorescence (IIF), an anti-centromere protein CenpG-serum was verified. Western blot of the protein extracts of 31 samples of breast cancer tissues and their normal (not cancerous ) tissues a little far away from them in the same individuals showed that, in the majority of the tests (71%), centromere protein CenpG over expressed in breast cancer tissues. And moreover, a kind of protein component whose molecular weight is 43 kd, and which can be recognized by anti-CenpG serum was found in two of the cancer samples. The results suggested that CenpG (together whith it, there may be other relative components),which has been found and named recently, may be related to cancer,and its differential expressing is probably related to malignant cell proliferation.

Microsatellite-Centromere Mapping in Japanese Scallop(Patinopecten yessoensis) Through Half-Tetrad Analysis in Gynogenetic Diploid Families

Gene-centromere mapping is an essential prerequisite for understanding the composition and structure of genomes. Half-tetrad analysis is a powerful tool for mapping genes and understanding chromosomal behavior during meiosis. The Japanese scallop(Patinopecten yessoensis), a cold-tolerant species inhabiting the northwestern Pacific coast, is a commercially important marine bivalve in Asian countries. In this study, inheritance of 32 informative microsatellite loci was examined in 70-h D-shaped larvae of three induced meiogynogenetic diploid families of P. yessoensis for centromere mapping using half-tetrad analysis. The ratio of gynogenetic diploids was proven to be 100%, 100% and 96% in the three families, respectively. Inheritance analysis in the control crosses showed that 51 of the 53 genotypic ratios observed were in accordance with Mendelian expectations at the 5% level after Bonferroni correction. Seven of the 32 microsatellite loci showed the existence of null alleles in control crosses. The second division segregation frequency(y) of the microsatellite loci ranged from 0.07 to 0.85 with a mean of 0.38, suggesting the existence of positive interference after a single chiasma formation in some chromosomes in the scallop. Microsatellite-centromere distances ranged from 4 c M to 42 c M under the assumption of complete interference. Information on the positions of centromeres in relation to the microsatellite loci will represent a contribution towards the assembly of genetic maps in the commercially important scallop species.

Cytological identification of an isotetrasomic in rice and its application to centromere mapping

The aneuploid with isochromosome or telochromosome is ideal material for exploring the position of centromere in lingkage map. FOr obtaining these aneuploids in rice, the primary trisomics from triplo-1 to triplo-12 and the aneuploids derived from a triploid of indica rice variety Zhongxian 3037 were carefully investigated. From the offsprings of triplo-10, a primary trisomic of chromosome 10 of the variety, an isotetrasomic "triplo-10-1" was obtained. Cytological investigation revealed that a pair of extra isochromosomes of triplo-10-1 were come from the short arm of chromosome 10. In the offsprings of the isotetrasomic, a secondary trisomic "triplo-10-2" 5 in which the extra- chromosome was an isochromosome derived from the short arm of chromosome 10, was identified. With the isotetrasomic, secondary trisomic, primary trisomic and diploid of variety Zhongxian 3037, different molecular markers were used for exploring the position of the centromere of chromosome 10. Based on the DNA dosage effect, it was verified that the molecular markers G1125, G333 and L169 were located on the short arm, G1084 and other 16 available molecular markers were on the long arm of chromosome 10. So the centromere of chromosome 10 was located somewhere betweenG1125 and G1084 according to the RFLP linkage map given by Kurata et al[1]. The distance from G1125 to G1084 was about 3.2cM.

Human chromosome pellicle antibody recognizing centromere protein-C(CENP-C),the main component of the kinetochore

Recently the antichromosome antisera from several scleroderma patients have been found to recognize the pellicle of metaphase and anaphase chromosomes. In order to identify the pellicle components, we used these antichromosome antisera to screen a human embryonic cDNA library. The sequences of the positive clones are identical to the cDNA gene sequence of CENP-C (centromere protein C), a human centromere autoantigen. This result suggusts that CENP-C is a component of the pellicle of human metaphase and anaphase chromosomes.

肺腺癌CENPA表达与CT征象和参数的关系研究

目的探讨着丝粒蛋白A(CENPA)表达水平与肺腺癌患者CT征象和参数的关系。方法进行肺部CT检查的46例肺腺癌患者为研究对象,根据CENPA表达水平,将46例患者分为CENPA-组(CENPA阴性,27例)和CENPA+组(CENPA阳性,19例)。记录并比较两组患者CT肺灌注参数值[血流量(BF)、血容量(BV)、表面通透性(PS)、平均通过时间(MTT)]以及CT征象个数。结果CENPA+组CT肺灌注BF(39.21±5.45)ml/(100 g·min)、BV(21.11±3.18)ml/100 g、PS(17.21±2.86)ml/(100 g·min)较CENPA-组的(19.22±3.50)ml/(100 g·min)、(4.89±1.97)ml/100 g、(4.19±2.34)ml/(100 g·min)升高,MTT(3.11±1.48)s较CENPA-组的(10.53±2.27)s短(P<0.05)。CENPA+组直接征象+间接征象<3个有3例,直接征象3~5个有2例,直接征象+间接征象3~5个有8例,直接征象>5个有2例,直接征象+间接征象>5个有4例,优于CENPA-组的6、13、5、2、1例(P<0.05)。结论肺腺癌患者CENPA表达水平与CT征象和参数相关,CENPA阳性表达的肺腺癌患者CT征象和参数明显更差,因此CENPA阳性表达可作为诊断肺腺癌发病的参考依据。

着丝粒蛋白A在宫颈癌组织中的表达及临床意义

目的:分析着丝粒蛋白A(centromere protein A,CENP-A)在宫颈癌中的表达情况,与临床病理参数相关性及临床意义。方法:GEPIA和HPA数据库分析CENP-A基因及蛋白在宫颈癌组织中的表达情况;免疫组化检测宫颈癌中CENP-A蛋白的表达水平,分析其与临床病理参数之间的关系;使用Linked Omics数据库寻找与CENP-A共表达基因,分析CENP-A可能参与宫颈癌发生发展的信号通路。结果:生物信息学分析显示宫颈癌组织中CENP-A基因和蛋白的表达水平明显高于癌旁组织(P<0.05);免疫组化结果显示宫颈癌组织CENP-A蛋白表达水平显著高于癌旁组织(P<0.05),且与患者的肿瘤分期及浸润程度相关。通过基因共表达分析,KIF2C、CDC20、FAM72B等基因与CENP-A表达呈正相关,PARM1、ABCA9和SYNE1等基因与CENP-A表达呈负相关(FDR<0.05),CENP-A基因参与了细胞周期、DNA复制等途径。结论:CENP-A在宫颈癌组织中高表达,与宫颈癌的预后以及发生发展相关。

着丝粒蛋白U在结直肠癌患者肠组织中的表达情况及临床意义

目的旨在探讨着丝粒蛋白U(centromere protein U,CENPU)在结直肠癌患者肠组织中的表达情况,并结合生物信息学分析其表达水平对结直肠癌患者预后的影响。方法通过实时荧光定量聚合酶链反应(quantitative real time polymerase chain reaction,qRT-PCR)、蛋白质免疫印迹(Western blot,WB)法以及免疫组织化学染色(immunohistochemistry,IHC)实验验证CENPU在组织中的表达情况。结合患者临床病例资料,通过单因素和多因素Cox回归分析CENPU的表达与结直肠癌患者临床病例参数的相关性;然后通过绘制受试操作者操作特征(receiver operating characteristic,ROC)曲线和Kaplan-Meier生存曲线,探究CENPU的表达对结直肠癌患者预后的预测作用。最后,通过生物信息学分析CENPU的表达对结直肠癌疾病进展影响的可能分子机制。结果通过qRT-PCR、WB法以及IHC实验均发现,与正常组织比较,CENPU在结直肠癌患者癌组织中表达显著升高。Cox回归分析表明CENPU的表达与患者的年龄和TNM分期显著相关,是影响患者预后的危险因素。Kaplan-Meier生存曲线分析表明:CENPU高表达的结直肠癌患者的生存率显著降低。ROC曲线结果表明:基于CENPU的表达建立的模型具有较高的预测结直肠癌患者预后的能力。生物信息学分析结果表明:CENPI、CENPN、CENPD、CENPK、CENPP、CENPM、CENPQ、CENPH、NDC80以及ITGB3BP这10个基因与CENPU基因具有相互作用关系;CENPU参与DNA修复、MYC/TARGETS/V1以及PI3K/AKT/MTOR等信号通路。结论结直肠癌患者癌组织中高表达的CENPU与患者的不良预后显著相关,提示CENPU有望成为结直肠癌患者早期诊断及预测预后的潜在靶点。

Wide hybridizations reveal the robustness of functional centromeres in Triticum-Aegilops species complex lines

The Triticum-Aegilops complex groups demonstrated high cross-affinity with each other to overcome the barriers of distant hybridization(Loureiro et al.,2023).Distant hybridization involves two distinct yet closely related events:hybridization and genome doubling.Previous studies have indicated that bursts of transposable elements(TEs)can occur as a consequence or concomitant to hybridization or genome duplication(Parisod et al.,2010).This raises an important scientific question regarding how the TEs-rich centromere region copes with genomic shock(McClintock,1984).The Triticum-Aegilops species complexes,particularly in the F1,So,and subsequent early generations resulting from successive selfcrossing,offer an opportunity to investigate whether the centromere environment undergoes reconstruction and the associated mechanisms that maintain genomic stability.

A centromere map based on super pan-genome highlights the structure and function of rice centromeres

Rice(Oryza sativa)is a significant crop worldwide with a genome shaped by various evolutionary factors.Rice centromeres are crucial for chromosome segregation,and contain some unreported genes.Due to the diverse and complex centromere region,a comprehensive understanding of rice centromere structure and function at the population level is needed.We constructed a high-quality centromere map based on the rice super pangenome consisting of a 251-accession panel comprising both cultivated and wild species of Asian and African rice.We showed that rice centromeres have diverse satellite repeat CentO,which vary across chromosomes and subpopulations,reflecting their distinct evolutionary patterns.We also revealed that long terminal repeats(LTRs),especially young Gypsy-type LTRs,are abundant in the peripheral CentO-enriched regions and drive rice centromere expansion and evolution.Furthermore,high-quality genome assembly and complete telomere-to-telomere(T2T)reference genome enable us to obtain more centromeric genome information despite mapping and cloning of centromere genes being challenging.We investigated the association between structural variations and gene expression in the rice centromere.A centromere gene,OsMAB,which positively regulates rice tiller number,was further confirmed by expression quantitative trait loci,haplotype analysis and clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein9 methods.By revealing the new insights into the evolutionary patterns and biological roles of rice centromeres,our finding will facilitate future research on centromere biology and crop improvement.

着丝粒蛋白H在肾上腺皮质癌的表达及对肾上腺皮质癌细胞活力和迁移的影响

目的:研究着丝粒蛋白H(CENP-H)在肾上腺皮质癌(ACC)的表达,分析其与疾病进展和预后的关系;探索敲减CENP-H表达对ACC细胞活力和迁移的影响。方法:应用GEPIA2数据库分析CENP-H在76例ACC患者和128例健康对照中的mRNA表达,并分析其在不同肿瘤分期的表达及与预后的相关性;运用UALCAN数据库进一步分析CENP-H在ACC不同肿瘤分期的mRNA表达及与预后的相关性;采用免疫组织化学染色检测CENP-H蛋白在ACC和正常肾上腺石蜡切片的表达。构建敲减CENP-H表达的siRNA(siCENP-H),将人ACC细胞系H295R分为siCENP-H组和siNC组;CCK-8实验检测细胞活力的变化,划痕实验检测细胞迁移能力的变化,Western blot实验检测CENP-H、p-ERK1/2、t-ERK1/2、p-P38、t-P38、p-JNK1/2和t-JNK1/2蛋白水平。结果:CENP-H在ACC中表达较健康对照显著升高,且与肿瘤分期和预后显著相关;CENP-H蛋白在ACC组织中的表达水平显著高于正常肾上腺;敲减CENP-H后,H295R细胞活力和迁移能力较对照组显著降低,p-P38和p-JNK1/2蛋白水平显著降低,以p-JNK1/2的降低尤为显著。结论:CENP-H在ACC中高表达;敲减CENP-H表达能抑制ACC细胞活力和迁移,其机制可能与抑制P38和JNK信号通路的活化有关。

着丝粒蛋白F、miR-1-3p在中晚期胃癌患者血清中的表达及与预后的相关性

目的探讨着丝粒蛋白F(CENPF)、miRNA-1-3p(miR-1-3p)在中晚期胃癌患者血清中的表达及与预后的相关性。方法选取2019年3月至2020年3月我院收治的60例中晚期胃癌患者即为研究组,同期在我院体检中心体检健康的志愿者60例为对照组。采用实时荧光定量PCR(qRT-PCR)法检测各组血清CENPF、miR-1-3p的表达水平;采用Pearson法分析血清中CENPF、miR-1-3p水平的相关性;采用Kaplan-Meier法分析CENPF、miR-1-3p表达与中晚期胃癌患者预后的关系;COX回归分析影响中晚期胃癌患者预后的危险因素。结果与对照组比较,研究组患者CENPF水平明显升高,miR-1-3p水平明显降低(P<0.05)。相关性分析结果显示,中晚期胃癌患者血清中CENPF和miR-1-3p水平呈负相关(r=-0.650,P<0.001)。不同TNM分期和淋巴结转移状况下CENPF、miR-1-3p水平比较差异有统计学意义(P<0.05)。CENPF高表达组患者3年生存率63.33%(19/30)显著低于低表达组93.33%(28/30)(χ^(2)=7.954,P<0.001);miR-1-3p高表达组患者3年生存率96.67%(29/30)显著高于低表达组60.00%(18/30)(χ^(2)=11.882,P=0.001)。多因素COX回归分析显示,TNM分期、淋巴结转移、CENPF、miR-1-3p表达是影响中晚期胃癌患者预后的危险因素(P<0.05)。结论中晚期胃癌患者血清中CENPF水平显著升高,miR-1-3p水平显著降低,二者与预后有关。

MRI联合血清CENPU、HSP90α在乳腺癌诊断中的临床价值

目的研究磁共振成像(MRI)联合血清着丝粒结合蛋白U(CENPU)、热休克蛋白90α(HSP90α)在乳腺癌诊断中的临床价值。方法选取2021年1月至2023年4月在本院就诊的疑似乳腺癌患者227例进行研究,以术后病理诊断结果作为金标准,将227例疑似乳腺癌患者分为乳腺癌组143例,良性肿瘤组84例。对所有患者进行MRI检查,酶联免疫法检测血清HSP90α水平,qRT-PCR法检测血清CENPU水平。ROC曲线分析血清CENPU、HSP90α水平对乳腺癌的诊断价值,四表格法分析MRI检测及其联合血清CENPU、HSP90α水平对乳腺癌的诊断价值。结果乳腺癌组血清CENPU、HSP90α水平显著高于良性肿瘤组,差异具有统计学意义(P<0.05)。血清CENPU水平诊断乳腺癌的AUC为0.739,敏感性为65.73%,特异性为76.19%;HSP90α水平诊断乳腺癌的AUC为0.767,敏感性为79.72%,特异性为64.29%。MRI检查结果显示,乳腺癌患者137例,良性肿瘤患者90例;检出浸润性导管癌50例,乳腺导管内原位癌47例,乳头状瘤27例,浸润性小叶癌13例。MRI检查乳腺癌的敏感性为76.92%,特异性为67.86%,准确度为73.57%;MRI检查对乳腺癌的诊断结果与病理诊断结果具有一致性(Kappa值=0.441,P<0.05)。M R I联合血清C E N P U、HSP90α水平诊断乳腺癌的敏感性为98.60%,特异性为63.10%,准确度为85.46%;三者联合对乳腺癌的诊断结果与病理诊断结果具有较好一致性(Kappa=0.664,P<0.05);MRI联合血清CENPU、HSP90α水平诊断乳腺癌的敏感性和准确度显著高于MRI、CENPU、HSP90α单独诊断(P<0.05)。结论MRI联合血清CENPU、HSP90α对乳腺癌诊断具有重要临床价值。

YTHDC1介导的CENPK调节人宫颈癌细胞侵袭和增殖

目的:找寻宫颈癌的预测、治疗靶点,探究靶点与N6-甲基腺苷(m6A)核酸修饰之间的联系及发挥的生物学功能。方法:在GEPIA2数据库提供的数据中筛选宫颈癌差异表达的基因。EdU实验、划痕实验以及Transwell实验分别检测着丝粒蛋白K(CENPK)对宫颈癌细胞增殖、迁移和侵袭能力的影响。RNA m6A修饰的定量测定结合蛋白质印迹法研究m6A修饰与CENPK高表达的关系。结果:CENPK在宫颈癌组织中上调(P<0.05)。沉默CENPK基因导致宫颈癌细胞增殖能力下降,抑制了细胞迁移和侵袭(P<0.01)。m6A阅读器YTH域包含蛋白1(YTHDC1)通过降低CENPK蛋白的表达在宫颈癌细胞中发挥作用(P<0.01)。结论:CENPK可能是一个潜在的宫颈癌治疗靶点。

着丝粒与动粒的功能、结构和动态组装

确保真核生物物种的遗传信息在亲代与子代之间忠实地传递是细胞有丝分裂的一项基本任务。着丝粒是一个特殊的染色体区域,对于在有丝分裂过程中介导姐妹染色单体的排列和分离至关重要。着丝粒身份确定是由含有着丝粒蛋白A(CENP-A)的核小体这种表观遗传机制决定的。CENP-A核小体为有丝分裂期内层动粒和外层动粒组装的关联提供了基础。本文回顾了着丝粒身份确定、内层动粒功能和组装以及外层动粒功能和组装。特别是,我们关注了组成型着丝粒关联网络(CCAN)结构活性关系的最新进展。CCAN结构信息为我们对着丝粒和动粒功能以及动态组装的理解提供了新的启示。

CENPK、IFI16表达与中晚期宫颈癌患者预后的关系

目的探讨着丝粒蛋白K(CENPK)、干扰素诱导核蛋白16(IFI16)表达与中晚期宫颈癌患者预后的关系。方法选取行同步放化疗的106例中晚期宫颈癌患者宫颈癌组织,以同期40例因子宫脱垂或子宫肌瘤行全子宫切除的正常宫颈组织为对照。免疫组织化学检测组织中CENPK、IFI16蛋白表达。比较不同临床病理特征中晚期宫颈癌患者CENPK、IFI16蛋白表达差异。Kaplan-Meier方法分析CENPK、IFI16表达与中晚期宫颈癌预后的关系。Cox回归分析影响中晚期宫颈癌患者预后的影响因素。结果宫颈癌组织中CENPK、IFI16蛋白阳性表达率分别为70.75%(75/106)、67.92%(72/106),高于正常宫颈组织的10.00%(4/40)、15.00%(6/40),差异有统计学意义(P均<0.05)。不同肿瘤最大径、FIGO分期及淋巴结转移中晚期宫颈癌患者癌组织中CENPK、IFI16表达比较差异有统计学意义(P均<0.05)。CENPK阳性者5年累积生存率低于CENPK阴性者(P<0.05),IFI16阳性者5年累积生存率亦低于CENPK阴性者(P<0.05)。FIGO分期Ⅲ~Ⅳ、淋巴结转移、CENPK阳性表达、IFI16阳性表达是影响中晚期宫颈癌患者预后的独立因素(P均<0.05)。结论中晚期宫颈癌组织中CENPK、IFI16表达升高,二者表达与肿瘤最大径、FIGO分期及淋巴结转移有关,是影响患者不良预后的独立因素。

CENPA表达与癌症的关系研究进展

异常的细胞分裂导致整个染色体不稳定是癌症发生的标志。染色体准确分离在细胞分裂中有着重要作用,而着丝粒则是染色体准确分离的关键。着丝粒蛋白A(CENPA)是一种着丝粒特异性组蛋白H3样变异基因,也是着丝粒蛋白家族中研究最广泛的因子,在多种癌症中过表达。CENPA过表达可能导致着丝粒异染色质沿染色体臂扩散,导致微管-动粒锚定缺陷,最终导致基因组不稳定。因此,探讨CENPA过表达与癌症进展的关系,对寻找新的靶向治疗方法有着重要意义。

着丝粒蛋白M在卵巢透明细胞癌中的表达及作用

目的探讨着丝粒蛋白M(centromere protein M,CENPM)在卵巢透明细胞癌(ovarian clear cell carcinoma,OCCC)组织中的表达及对OCCC中ES2细胞株增殖、迁移、凋亡的影响和作用机制。方法采用HE染色观察OCCC组织病理改变;免疫组织化学法检测OCCC组织及癌旁组织CENPM的表达;si-CENPM转染ES2细胞;Western blot检测CENPM蛋白的表达;CCK-8法、细胞划痕实验检测细胞增殖和迁移能力;流式细胞仪评价CENPM对细胞凋亡和周期的影响。结果与癌旁组织相比,OCCC组织中CENPM高表达(P<0.01);转染si-CENPM#B组ES2细胞CENPM蛋白表达水平较si-NC组降低(P<0.01);与Control组和si-NC组比较,敲减CENPM后ES2细胞的增殖、迁移能力下降(P均<0.05);敲减CENPM后ES2细胞凋亡率增高,阻滞ES2细胞的周期在S期(P均<0.05)。结论下调CENPM可抑制人OCCC中ES2细胞株的增殖、迁移并促进细胞凋亡,提示CENPM参与了OCCC的发生发展。

着丝粒蛋白F在胶质瘤组织中的表达及预后分析

目的探究着丝粒蛋白F(CENPF)在脑胶质瘤中的表达及预后分析。方法通过对癌症基因组图谱(TCGA)和中国脑胶质瘤图谱(CGGA)数据库进行生物信息分析,比较CENPF在低级别胶质瘤(LGG)、胶质母细胞瘤(GBM)和癌旁组织中的表达差异以及与患者预后之间的关系,并在数据库中对CENPF mRNA与P53、Ki-67以及IDH-1分型进行相关性分析。采用实时荧光定量PCR(qRT-PCR)法检测CENPF mRNA表达水平,免疫组织化学法和Western blotting法检测癌旁组织和不同级别胶质瘤组织中CENPF表达水平。多因素COX分析CENPF与临床病理参数及患者预后的关系,并绘制Kaplan-Meier生存曲线。利用TCGA数据库对CENPF进行KEGG富集分析,探索该基因在胶质瘤中发展中可能参与的信号通路。结果CENPF表达水平与胶质瘤WHO分级呈正相关,且CENPF高表达的胶质瘤患者生存时间短于低表达患者。数据库相关性分析显示CENPF mRNA与P53、Ki-67以及IDH-1野生型呈正相关。qRT-PCR实验结果表明CENPF mRNA在胶质瘤组织中表达增高,免疫组织化学和Western blotting实验结果表明CENPF表达与WHO等级呈正相关。临床病理参数分析表明在胶质瘤组织中CENPF表达情况与胶质瘤WHO分级(P=0.002)、P53(P=0.016)、Ki-67(P<0.001)表达有关。多因素COX分析显示WHO分级(P<0.001)、CENPF表达(P=0.008)、P53(P=0.003)和Ki-67(P=0.006)表达为胶质瘤患者预后不良的危险因素。Kaplan-Meier生存曲线表明CENPF高表达的胶质瘤患者生存时间短于低表达患者(P<0.0001)。KEGG富集分析显示CENPF在参与细胞周期、DNA复制、WNT/beta-catenin、mTORC1等通路中具有显著富集。结论CENPF在胶质瘤组织中表达增高,其表达与WHO分级、Ki-67以及P53分型相关;CENPF可作为判断胶质瘤患者预后的生物标志物。