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Identification and Fine Mapping of a Gene Related to Pale Green Leaf Phenotype near the Centromere Region in Rice(Oryza sativa) 被引量:11
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作者 ZHU Li LiuWenzhen WU Chao LUAN Wei jiang Fu Ya ping Hu Guo cheng SI Hua min SUN Zong xiu 《Rice science》 SCIE 2007年第3期172-180,共9页
A thermo-insensitive pale green leaf mutant (pgl2) was isolated from T-DNA inserted transgenic lines of rice (Oryza sativa L. subsp, japonica cv. Nipponbare). Genetic analysis indicated that the phenotype was caus... A thermo-insensitive pale green leaf mutant (pgl2) was isolated from T-DNA inserted transgenic lines of rice (Oryza sativa L. subsp, japonica cv. Nipponbare). Genetic analysis indicated that the phenotype was caused by a recessive mutation in a single nuclear-encoded gene. To map the PGL2gene, an F2 population was constructed by crossing the mutant with Longtefu (Oryza sativa L. subsp, indica). The PGL2 locus was roughly linked to SSR marker RM331 on chromosome 8. To finely map the gene, 14 new InDel markers were developed around the marker, and PGL2 was further mapped to a 2.37 Mb centromeric region. Analysis on chlorophyll contents of leaves showed that there was no obvious difference between the mutant and the wild type in total chlorophyll (Chl) content, while the ratio of Chl a / Chl b in the mutant was only about 1, which was distinctly lower than that in the wild type, suggesting that the PGL2 gene was related to the conversion between Chl a and Chl b. Moreover, the method of primer design around the centromeric region was discussed, which would provide insight into fine mapping of the functional genes in plant centromeres. 展开更多
关键词 centromere GENE fine mapping pale green leaf mutant chlorophyll a chlorophyll b RICE
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Differential Expressing of Centromere Protein CenpG in Breast Cancer 被引量:3
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作者 梁前进 Lu Xiangfeng +3 位作者 Cheng Xiaolei Zhang Huanxiang He Dacheng WANG Yongchao 《High Technology Letters》 EI CAS 2002年第1期1-4,共4页
Using indirect immunofluorescence (IIF), an anti-centromere protein CenpG-serum was verified. Western blot of the protein extracts of 31 samples of breast cancer tissues and their normal (not cancerous ) tissues a lit... Using indirect immunofluorescence (IIF), an anti-centromere protein CenpG-serum was verified. Western blot of the protein extracts of 31 samples of breast cancer tissues and their normal (not cancerous ) tissues a little far away from them in the same individuals showed that, in the majority of the tests (71%), centromere protein CenpG over expressed in breast cancer tissues. And moreover, a kind of protein component whose molecular weight is 43 kd, and which can be recognized by anti-CenpG serum was found in two of the cancer samples. The results suggested that CenpG (together whith it, there may be other relative components),which has been found and named recently, may be related to cancer,and its differential expressing is probably related to malignant cell proliferation. 展开更多
关键词 Breast cancer centromere protein CenpG
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Overexpression of centromere protein H is significantly associated with breast cancer progression and overall patient survival 被引量:4
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作者 Wen-Ting Liao Yan Feng +4 位作者 Men-Lin Li Guang-Lin Liu Man-Zhi Li Mu-Sheng Zeng Li-Bing Song 《Chinese Journal of Cancer》 SCIE CAS CSCD 北大核心 2011年第9期627-637,共11页
Breast cancer is one of the leading causes of cancer death worldwide.This study aimed to analyze the expression of centromere protein H(CENP-H) in breast cancer and to correlate it with clinicopathologic data,includin... Breast cancer is one of the leading causes of cancer death worldwide.This study aimed to analyze the expression of centromere protein H(CENP-H) in breast cancer and to correlate it with clinicopathologic data,including patient survival.Using reverse transcription-polymerase chain reaction and Western blotting to detect the expression of CENP-H in normal mammary epithelial cells,immortalized mammary epithelial cell lines,and breast cancer cell lines,we observed that the mRNA and protein levels of CENP-H were higher in breast cancer cell lines and in immortalized mammary epithelial cells than in normal mammary epithelial cells.We next examined CENP-H expression in 307 paraffin-embedded archived samples of clinicopathologically characterized breast cancer using immunohistochemistry,and detected high CENP-H expression in 134(43.6%) samples.Statistical analysis showed that CENP-H expression was related with clinical stage(P = 0.001),T classification(P = 0.032),N classification(P = 0.018),and Ki-67(P < 0.001).Patients with high CENP-H expression had short overall survival.Multivariate analysis showed that CENP-H expression was an independent prognostic indicator for patient survival.Our results suggest that CENP-H protein is a valuable marker of breast cancer progression and prognosis. 展开更多
关键词 着丝粒蛋白 乳腺癌 过度表达 WESTERN印迹法 乳腺上皮细胞 细胞永生化 聚合酶链反应 临床病理
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Cytological identification of an isotetrasomic in rice and its application to centromere mapping
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作者 CHENG ZHU KUAN HENG XIU YU +2 位作者 CHANG JIE YAN LI HUANG ZHU MING HONG GU(Department of Agronomy, Agriculture College, Yangzhou University, Yangzhou 225009)(Institute of Genetics, Chinese Academy of Sciences,Beijing 100101) 《Cell Research》 SCIE CAS CSCD 1997年第1期31-38,共8页
The aneuploid with isochromosome or telochromosome is ideal material for exploring the position of centromere in lingkage map. FOr obtaining these aneuploids in rice, the primary trisomics from triplo-1 to triplo-12 a... The aneuploid with isochromosome or telochromosome is ideal material for exploring the position of centromere in lingkage map. FOr obtaining these aneuploids in rice, the primary trisomics from triplo-1 to triplo-12 and the aneuploids derived from a triploid of indica rice variety Zhongxian 3037 were carefully investigated. From the offsprings of triplo-10, a primary trisomic of chromosome 10 of the variety, an isotetrasomic "triplo-10-1" was obtained. Cytological investigation revealed that a pair of extra isochromosomes of triplo-10-1 were come from the short arm of chromosome 10. In the offsprings of the isotetrasomic, a secondary trisomic "triplo-10-2" 5 in which the extra- chromosome was an isochromosome derived from the short arm of chromosome 10, was identified. With the isotetrasomic, secondary trisomic, primary trisomic and diploid of variety Zhongxian 3037, different molecular markers were used for exploring the position of the centromere of chromosome 10. Based on the DNA dosage effect, it was verified that the molecular markers G1125, G333 and L169 were located on the short arm, G1084 and other 16 available molecular markers were on the long arm of chromosome 10. So the centromere of chromosome 10 was located somewhere betweenG1125 and G1084 according to the RFLP linkage map given by Kurata et al[1]. The distance from G1125 to G1084 was about 3.2cM. 展开更多
关键词 RICE isotetrasomic centromere mapping
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Human chromosome pellicle antibody recognizing centromere protein-C(CENP-C),the main component of the kinetochore
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作者 XIE YONG ZU MEI NI +3 位作者 JIAN REN GU PHIL WONG WEN QING WU GUO WEI XU(Hong Kong University of Science and Technology,Department of Biology, Hong Kong) (Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai) (Shanghai Cancer Institute, Nation 《Cell Research》 SCIE CAS CSCD 1997年第1期13-19,共7页
Recently the antichromosome antisera from several scleroderma patients have been found to recognize the pellicle of metaphase and anaphase chromosomes. In order to identify the pellicle components, we used these antic... Recently the antichromosome antisera from several scleroderma patients have been found to recognize the pellicle of metaphase and anaphase chromosomes. In order to identify the pellicle components, we used these antichromosome antisera to screen a human embryonic cDNA library. The sequences of the positive clones are identical to the cDNA gene sequence of CENP-C (centromere protein C), a human centromere autoantigen. This result suggusts that CENP-C is a component of the pellicle of human metaphase and anaphase chromosomes. 展开更多
关键词 Human antibody scleroderma CENP-C (centromere protein C) METAPHASE chromosome pellicle indirect immunofluorescent staining
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血清抗着丝粒蛋白F抗体在乳腺癌中的临床价值探讨
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作者 王艳萍 唐笛娇 +2 位作者 努尔比耶·买买提依力 邹麟 陈瀑 《重庆医科大学学报》 CAS CSCD 北大核心 2024年第9期1188-1192,共5页
目的:探讨血清抗着丝粒蛋白F抗体(anti-centromere protein F antibody,anti-CENPF)在乳腺癌中的临床价值。方法:收集100例初诊乳腺癌(breast cancer,BC)患者、40例乳腺良性疾病(non breast cancer,non-BC)患者和40名健康体检者(healthy... 目的:探讨血清抗着丝粒蛋白F抗体(anti-centromere protein F antibody,anti-CENPF)在乳腺癌中的临床价值。方法:收集100例初诊乳腺癌(breast cancer,BC)患者、40例乳腺良性疾病(non breast cancer,non-BC)患者和40名健康体检者(healthy control,HC)血清,采用酶联免疫吸附试验检测血清中的anti-CENPF水平,同时化学发光法测定血清糖类抗原153(carbohydrate antigen 153,CA153)的水平。结果:BC组血清anti-CENPF水平高于non-BC组和HC组,差异有统计学意义(P<0.05)。anti-CENPF和CA153诊断乳腺癌中的曲线下面积(area under the curve,AUC)分别为0.714和0.672,两者联合检测的AUC为0.739。有淋巴结转移、远处转移或者人表皮生长因子受体2(human epidermal growth factor receptor-2,HER-2)阴性的乳腺癌患者血清anti-CENPF浓度明显升高(P<0.05)。不同肿瘤大小、临床分期、分子分型乳腺癌患者血清anti-CENPF水平比较,差异有统计学意义(P<0.05)。组间比较显示Ⅳ期和Ⅲ期血清anti-CENPF浓度高于Ⅰ期和Ⅱ期,HER-2过表达型、Luminal B型血清anti-CENPF水平低于三阴性乳腺癌(triple negative breast cancer,TNBC)患者。雌激素受体(receptors estrogen,ER)阳性的乳腺癌患者中,HER-2阴性组的anti-CENPF浓度高于HER-2阳性组,差异有统计学意义(P=0.026)。结论:血清antiCENPF在乳腺癌的诊断、临床分期及分子分型中发挥了重要作用,其水平可能与乳腺癌预后呈负相关,有望成为乳腺癌潜在的疾病标志物。 展开更多
关键词 抗着丝粒蛋白F抗体 乳腺癌 诊断 分期 预后
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肺腺癌CENPA表达与CT征象和参数的关系研究
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作者 万静茹 孙宇 +1 位作者 王东旭 郝利国 《中国现代药物应用》 2024年第7期50-53,共4页
目的探讨着丝粒蛋白A(CENPA)表达水平与肺腺癌患者CT征象和参数的关系。方法进行肺部CT检查的46例肺腺癌患者为研究对象,根据CENPA表达水平,将46例患者分为CENPA-组(CENPA阴性,27例)和CENPA+组(CENPA阳性,19例)。记录并比较两组患者CT... 目的探讨着丝粒蛋白A(CENPA)表达水平与肺腺癌患者CT征象和参数的关系。方法进行肺部CT检查的46例肺腺癌患者为研究对象,根据CENPA表达水平,将46例患者分为CENPA-组(CENPA阴性,27例)和CENPA+组(CENPA阳性,19例)。记录并比较两组患者CT肺灌注参数值[血流量(BF)、血容量(BV)、表面通透性(PS)、平均通过时间(MTT)]以及CT征象个数。结果CENPA+组CT肺灌注BF(39.21±5.45)ml/(100 g·min)、BV(21.11±3.18)ml/100 g、PS(17.21±2.86)ml/(100 g·min)较CENPA-组的(19.22±3.50)ml/(100 g·min)、(4.89±1.97)ml/100 g、(4.19±2.34)ml/(100 g·min)升高,MTT(3.11±1.48)s较CENPA-组的(10.53±2.27)s短(P<0.05)。CENPA+组直接征象+间接征象<3个有3例,直接征象3~5个有2例,直接征象+间接征象3~5个有8例,直接征象>5个有2例,直接征象+间接征象>5个有4例,优于CENPA-组的6、13、5、2、1例(P<0.05)。结论肺腺癌患者CENPA表达水平与CT征象和参数相关,CENPA阳性表达的肺腺癌患者CT征象和参数明显更差,因此CENPA阳性表达可作为诊断肺腺癌发病的参考依据。 展开更多
关键词 肺腺癌 着丝粒蛋白A CT征象 关系研究
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CENPA、GRHL2与乳腺癌临床病理特征的关系及其在预后评估中的临床价值
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作者 王晶晶 沈苏琴 《国际检验医学杂志》 CAS 2024年第19期2316-2322,共7页
目的探讨着丝粒蛋白A(CENPA)和粒状头样2(GRHL2)在乳腺癌组织中的表达,构建乳腺癌预后模型并分析其临床应用价值。方法采用基因表达谱交互分析(GEPIA)数据库分析1085例乳腺癌组织和291例正常组织中CENPA和GRHL2表达,Kaplan-Meier Plotte... 目的探讨着丝粒蛋白A(CENPA)和粒状头样2(GRHL2)在乳腺癌组织中的表达,构建乳腺癌预后模型并分析其临床应用价值。方法采用基因表达谱交互分析(GEPIA)数据库分析1085例乳腺癌组织和291例正常组织中CENPA和GRHL2表达,Kaplan-Meier Plotter在线网站分析CENPA和GRHL2表达与乳腺癌患者生存情况的关系。选取2018年1月至2020年6月在该院接受治疗的84例原发性单侧乳腺癌患者作为研究对象,免疫组化法检测CENPA和GRHL2表达并分析其与乳腺癌临床病理特征的关系,Spearman相关分析CENPA、GRHL2与糖类抗原153(CA153)的相关性,受试者工作特征(ROC)曲线分析CENPA、GRHL2和CA153对乳腺癌预后评估的效能;Cox比例风险回归模型分析乳腺癌预后的危险因素。结果GEPIA数据库提示乳腺癌组织中CENPA、GRHL2表达水平高于正常组织(P<0.05);Kaplan-Meier Plotter分析表明,CENPA、GRHL2表达水平升高,患者生存率降低(Log-rank P<0.001);CENPA、GRHL2表达与CA153呈正相关(r=0.410、0.602,P<0.001),三者联合检测对乳腺癌诊断的曲线下面积为0.878;CENPA和GRHL2表达与乳腺癌患者TNM分期、组织学分级、淋巴结转移及表皮生长因子受体-2阳性有关(P<0.05);Cox回归分析显示,TNM分期Ⅲ期、组织学分级Ⅲ级、淋巴结转移及CENPA、GRHL2高表达是乳腺癌预后的独立危险因素(P<0.05)。结论CENPA和GRHL2是乳腺癌预后评估有价值的生物标志物,与CA153联合检测对乳腺癌预后具有较高的预测价值。 展开更多
关键词 乳腺癌 着丝粒蛋白A 粒状头样2 预测模型
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High-quality Gossypium hirsutum and Gossypium barbadense genome assemblies reveal the landscape and evolution of centromeres 被引量:2
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作者 Xing Chang Xin He +8 位作者 Jianying Li Zhenping Liu Ruizhen Pi Xuanxuan Luo Ruipeng Wang Xiubao Hu Sifan Lu Xianlong Zhang Maojun Wang 《Plant Communications》 SCIE CSCD 2024年第2期135-150,共16页
Centromere positioning and organization are crucial for genome evolution;however,research on centro-mere biology is largely influenced by the quality of available genome assemblies.Here,we combined Oxford Nanopore and... Centromere positioning and organization are crucial for genome evolution;however,research on centro-mere biology is largely influenced by the quality of available genome assemblies.Here,we combined Oxford Nanopore and Pacific Biosciences technologies to de novo assemble two high-quality reference genomes for Gossypium hirsutum(TM-1)and Gossypium barbadense(3-79).Compared with previously published reference genomes,our assemblies show substantial improvements,with the contig N50 improved by 4.6-fold and 5.6-fold,respectively,and thus represent the most complete cotton genomes to date.These high-quality reference genomes enable us to characterize 14 and 5 complete centromeric regions for G.hirsutum and G.barbadense,respectively.Our data revealed that the centromeres of allotetraploid cotton are occupied by members of the centromeric repeat for maize(CRM)and Tekay long terminal repeat families,and the CRM family reshapes the centromere structure of the At subgenome after polyploidization.These two intertwined families have driven the convergent evolution of centromeres between the two subgenomes,ensuring centromere function and genome stability.In addition,the reposi-tioning and high sequence divergence of centromeres between G.hirsutum and G.barbadense have contributed to speciation and centromere diversity.This study sheds light on centromere evolution in a sig-nificant crop and provides an alternative approach for exploring the evolution of polyploid plants. 展开更多
关键词 genome assembly centromere architecture convergent evolution POLYPLOIDIZATION
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A centromere map based on super pan-genome highlights the structure and function of rice centromeres 被引量:1
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作者 Yang Lv Congcong Liu +32 位作者 Xiaoxia Li Yueying Wang Huiying He Wenchuang He Wu Chen Longbo Yang Xiaofan Dai Xinglan Cao Xiaoman Yu Jiajia Liu Bin Zhang Hua Wei Hong Zhang Hongge Qian Chuanlin Shi Yue Leng Xiangpei Liu Mingliang Guo Xianmeng Wang Zhipeng Zhang Tianyi Wang Bintao Zhang Qiang Xu Yan Cui Qianqian Zhang Qiaoling Yuan Noushin Jahan Jie Ma Xiaoming Zheng Yongfeng Zhou Qian Qian Longbiao Guo Lianguang Shang 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2024年第2期196-207,共12页
Rice(Oryza sativa)is a significant crop worldwide with a genome shaped by various evolutionary factors.Rice centromeres are crucial for chromosome segregation,and contain some unreported genes.Due to the diverse and c... Rice(Oryza sativa)is a significant crop worldwide with a genome shaped by various evolutionary factors.Rice centromeres are crucial for chromosome segregation,and contain some unreported genes.Due to the diverse and complex centromere region,a comprehensive understanding of rice centromere structure and function at the population level is needed.We constructed a high-quality centromere map based on the rice super pangenome consisting of a 251-accession panel comprising both cultivated and wild species of Asian and African rice.We showed that rice centromeres have diverse satellite repeat CentO,which vary across chromosomes and subpopulations,reflecting their distinct evolutionary patterns.We also revealed that long terminal repeats(LTRs),especially young Gypsy-type LTRs,are abundant in the peripheral CentO-enriched regions and drive rice centromere expansion and evolution.Furthermore,high-quality genome assembly and complete telomere-to-telomere(T2T)reference genome enable us to obtain more centromeric genome information despite mapping and cloning of centromere genes being challenging.We investigated the association between structural variations and gene expression in the rice centromere.A centromere gene,OsMAB,which positively regulates rice tiller number,was further confirmed by expression quantitative trait loci,haplotype analysis and clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein9 methods.By revealing the new insights into the evolutionary patterns and biological roles of rice centromeres,our finding will facilitate future research on centromere biology and crop improvement. 展开更多
关键词 centromere super pan-genome CentoO satellite repeat RICE
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着丝粒蛋白A在宫颈癌组织中的表达及临床意义
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作者 汪煜琳 曹文静 +2 位作者 翟雨晴 原江水 宋卫青 《现代肿瘤医学》 CAS 2024年第10期1882-1886,共5页
目的:分析着丝粒蛋白A(centromere protein A,CENP-A)在宫颈癌中的表达情况,与临床病理参数相关性及临床意义。方法:GEPIA和HPA数据库分析CENP-A基因及蛋白在宫颈癌组织中的表达情况;免疫组化检测宫颈癌中CENP-A蛋白的表达水平,分析其... 目的:分析着丝粒蛋白A(centromere protein A,CENP-A)在宫颈癌中的表达情况,与临床病理参数相关性及临床意义。方法:GEPIA和HPA数据库分析CENP-A基因及蛋白在宫颈癌组织中的表达情况;免疫组化检测宫颈癌中CENP-A蛋白的表达水平,分析其与临床病理参数之间的关系;使用Linked Omics数据库寻找与CENP-A共表达基因,分析CENP-A可能参与宫颈癌发生发展的信号通路。结果:生物信息学分析显示宫颈癌组织中CENP-A基因和蛋白的表达水平明显高于癌旁组织(P<0.05);免疫组化结果显示宫颈癌组织CENP-A蛋白表达水平显著高于癌旁组织(P<0.05),且与患者的肿瘤分期及浸润程度相关。通过基因共表达分析,KIF2C、CDC20、FAM72B等基因与CENP-A表达呈正相关,PARM1、ABCA9和SYNE1等基因与CENP-A表达呈负相关(FDR<0.05),CENP-A基因参与了细胞周期、DNA复制等途径。结论:CENP-A在宫颈癌组织中高表达,与宫颈癌的预后以及发生发展相关。 展开更多
关键词 宫颈癌 着丝粒蛋白A 免疫组化 生物信息学分析
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着丝粒蛋白U在结直肠癌患者肠组织中的表达情况及临床意义
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作者 王若淳 黄伟 +7 位作者 葛思佳 陈婧 宣晗 颜杨 姜佳炜 肖明兵 陆翠华 刘肇修 《中国现代医生》 2024年第11期1-6,共6页
目的旨在探讨着丝粒蛋白U(centromere protein U,CENPU)在结直肠癌患者肠组织中的表达情况,并结合生物信息学分析其表达水平对结直肠癌患者预后的影响。方法通过实时荧光定量聚合酶链反应(quantitative real time polymerase chain reac... 目的旨在探讨着丝粒蛋白U(centromere protein U,CENPU)在结直肠癌患者肠组织中的表达情况,并结合生物信息学分析其表达水平对结直肠癌患者预后的影响。方法通过实时荧光定量聚合酶链反应(quantitative real time polymerase chain reaction,qRT-PCR)、蛋白质免疫印迹(Western blot,WB)法以及免疫组织化学染色(immunohistochemistry,IHC)实验验证CENPU在组织中的表达情况。结合患者临床病例资料,通过单因素和多因素Cox回归分析CENPU的表达与结直肠癌患者临床病例参数的相关性;然后通过绘制受试操作者操作特征(receiver operating characteristic,ROC)曲线和Kaplan-Meier生存曲线,探究CENPU的表达对结直肠癌患者预后的预测作用。最后,通过生物信息学分析CENPU的表达对结直肠癌疾病进展影响的可能分子机制。结果通过qRT-PCR、WB法以及IHC实验均发现,与正常组织比较,CENPU在结直肠癌患者癌组织中表达显著升高。Cox回归分析表明CENPU的表达与患者的年龄和TNM分期显著相关,是影响患者预后的危险因素。Kaplan-Meier生存曲线分析表明:CENPU高表达的结直肠癌患者的生存率显著降低。ROC曲线结果表明:基于CENPU的表达建立的模型具有较高的预测结直肠癌患者预后的能力。生物信息学分析结果表明:CENPI、CENPN、CENPD、CENPK、CENPP、CENPM、CENPQ、CENPH、NDC80以及ITGB3BP这10个基因与CENPU基因具有相互作用关系;CENPU参与DNA修复、MYC/TARGETS/V1以及PI3K/AKT/MTOR等信号通路。结论结直肠癌患者癌组织中高表达的CENPU与患者的不良预后显著相关,提示CENPU有望成为结直肠癌患者早期诊断及预测预后的潜在靶点。 展开更多
关键词 结直肠癌 着丝粒蛋白U 富集分析
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植物单倍体诱导的方法、应用及展望
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作者 杨延铭 王娜 《自然杂志》 CAS 2024年第4期285-298,共14页
单倍体育种技术可以极大地提高育种效率。单倍体诱导的产生主要包括体外配子体组织培养和体内染色体消除。文章综述了体内单倍体诱导的几种策略及其发展和应用,还对目前单倍体诱导系的作用机制进行了介绍,并重点介绍了着丝粒蛋白CENH3... 单倍体育种技术可以极大地提高育种效率。单倍体诱导的产生主要包括体外配子体组织培养和体内染色体消除。文章综述了体内单倍体诱导的几种策略及其发展和应用,还对目前单倍体诱导系的作用机制进行了介绍,并重点介绍了着丝粒蛋白CENH3介导的单倍体诱导机制。 展开更多
关键词 单倍体 染色体消除 CENH3 STOCK6 着丝粒
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着丝粒蛋白F、miR-1-3p在中晚期胃癌患者血清中的表达及与预后的相关性 被引量:2
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作者 赵健 刘松杰 +3 位作者 张观朝 沈裕厚 李凤臣 徐兵 《实用医学杂志》 CAS 北大核心 2024年第3期365-370,共6页
目的探讨着丝粒蛋白F(CENPF)、miRNA-1-3p(miR-1-3p)在中晚期胃癌患者血清中的表达及与预后的相关性。方法选取2019年3月至2020年3月我院收治的60例中晚期胃癌患者即为研究组,同期在我院体检中心体检健康的志愿者60例为对照组。采用实... 目的探讨着丝粒蛋白F(CENPF)、miRNA-1-3p(miR-1-3p)在中晚期胃癌患者血清中的表达及与预后的相关性。方法选取2019年3月至2020年3月我院收治的60例中晚期胃癌患者即为研究组,同期在我院体检中心体检健康的志愿者60例为对照组。采用实时荧光定量PCR(qRT-PCR)法检测各组血清CENPF、miR-1-3p的表达水平;采用Pearson法分析血清中CENPF、miR-1-3p水平的相关性;采用Kaplan-Meier法分析CENPF、miR-1-3p表达与中晚期胃癌患者预后的关系;COX回归分析影响中晚期胃癌患者预后的危险因素。结果与对照组比较,研究组患者CENPF水平明显升高,miR-1-3p水平明显降低(P<0.05)。相关性分析结果显示,中晚期胃癌患者血清中CENPF和miR-1-3p水平呈负相关(r=-0.650,P<0.001)。不同TNM分期和淋巴结转移状况下CENPF、miR-1-3p水平比较差异有统计学意义(P<0.05)。CENPF高表达组患者3年生存率63.33%(19/30)显著低于低表达组93.33%(28/30)(χ^(2)=7.954,P<0.001);miR-1-3p高表达组患者3年生存率96.67%(29/30)显著高于低表达组60.00%(18/30)(χ^(2)=11.882,P=0.001)。多因素COX回归分析显示,TNM分期、淋巴结转移、CENPF、miR-1-3p表达是影响中晚期胃癌患者预后的危险因素(P<0.05)。结论中晚期胃癌患者血清中CENPF水平显著升高,miR-1-3p水平显著降低,二者与预后有关。 展开更多
关键词 着丝粒蛋白F 微小核糖核酸-1-3p 中晚期胃癌 预后
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着丝粒蛋白H在肾上腺皮质癌的表达及对肾上腺皮质癌细胞活力和迁移的影响 被引量:1
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作者 邹存茹 王丹 +5 位作者 张玉 刘呈悦 蒋荷坪 何文玺 张鑫源 苏文霞 《中国病理生理杂志》 CAS CSCD 北大核心 2024年第3期404-410,共7页
目的:研究着丝粒蛋白H(CENP-H)在肾上腺皮质癌(ACC)的表达,分析其与疾病进展和预后的关系;探索敲减CENP-H表达对ACC细胞活力和迁移的影响。方法:应用GEPIA2数据库分析CENP-H在76例ACC患者和128例健康对照中的mRNA表达,并分析其在不同肿... 目的:研究着丝粒蛋白H(CENP-H)在肾上腺皮质癌(ACC)的表达,分析其与疾病进展和预后的关系;探索敲减CENP-H表达对ACC细胞活力和迁移的影响。方法:应用GEPIA2数据库分析CENP-H在76例ACC患者和128例健康对照中的mRNA表达,并分析其在不同肿瘤分期的表达及与预后的相关性;运用UALCAN数据库进一步分析CENP-H在ACC不同肿瘤分期的mRNA表达及与预后的相关性;采用免疫组织化学染色检测CENP-H蛋白在ACC和正常肾上腺石蜡切片的表达。构建敲减CENP-H表达的siRNA(siCENP-H),将人ACC细胞系H295R分为siCENP-H组和siNC组;CCK-8实验检测细胞活力的变化,划痕实验检测细胞迁移能力的变化,Western blot实验检测CENP-H、p-ERK1/2、t-ERK1/2、p-P38、t-P38、p-JNK1/2和t-JNK1/2蛋白水平。结果:CENP-H在ACC中表达较健康对照显著升高,且与肿瘤分期和预后显著相关;CENP-H蛋白在ACC组织中的表达水平显著高于正常肾上腺;敲减CENP-H后,H295R细胞活力和迁移能力较对照组显著降低,p-P38和p-JNK1/2蛋白水平显著降低,以p-JNK1/2的降低尤为显著。结论:CENP-H在ACC中高表达;敲减CENP-H表达能抑制ACC细胞活力和迁移,其机制可能与抑制P38和JNK信号通路的活化有关。 展开更多
关键词 肾上腺皮质癌 着丝粒蛋白H 细胞活力 细胞迁移 MAPK信号通路
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Unveiling the distinctive traits of functional rye centromeres:minisatellites,retrotransposons,and R-loop formation
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作者 Chang Liu Shulan Fu +7 位作者 Congyang Yi Yang Liu Yuhong Huang Xianrui Guo Kaibiao Zhang Qian Liu James A.Birchler Fangpu Han 《Science China(Life Sciences)》 SCIE CAS CSCD 2024年第9期1989-2002,共14页
Centromeres play a vital role in cellular division by facilitating kinetochore assembly and spindle attachments.Despite their conserved functionality,centromeric DNA sequences exhibit rapid evolution,presenting divers... Centromeres play a vital role in cellular division by facilitating kinetochore assembly and spindle attachments.Despite their conserved functionality,centromeric DNA sequences exhibit rapid evolution,presenting diverse sizes and compositions across species.The functional significance of rye centromeric DNA sequences,particularly in centromere identity,remains unclear.In this study,we comprehensively characterized the sequence composition and organization of rye centromeres.Our findings revealed that these centromeres are primarily composed of long terminal repeat retrotransposons(LTR-RTs)and interspersed minisatellites.We systematically classified LTR-RTs into five categories,highlighting the prevalence of younger CRS1,CRS2,and CRS3 of CRSs(centromeric retrotransposons of Secale cereale)were primarily located in the core centromeres and exhibited a higher association with CENH3 nucleosomes.The minisatellites,mainly derived from retrotransposons,along with CRSs,played a pivotal role in establishing functional centromeres in rye.Additionally,we observed the formation of R-loops at specific regions of CRS1,CRS2,and CRS3,with both rye pericentromeres and centromeres exhibiting enrichment in R-loops.Notably,these R-loops selectively formed at binding regions of the CENH3 nucleosome in rye centromeres,suggesting a potential role in mediating the precise loading of CENH3 to centromeres and contributing to centromere specification.Our work provides insights into the DNA sequence composition,distribution,and potential function of R-loops in rye centromeres.This knowledge contributes valuable information to understanding the genetics and epigenetics of rye centromeres,offering implications for the development of synthetic centromeres in future plant modifications and beyond. 展开更多
关键词 centromere LTR-RTs MINISATELLITE RYE R-loop
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Wide hybridizations reveal the robustness of functional centromeres in Triticum-Aegilops species complex lines
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作者 Yuhong Huang Qinghua Shi +5 位作者 Chen Zhou Chunhui Wang Yang Liu Congyang Yi Handong Su Fangpu Han 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2024年第5期570-573,共4页
The Triticum-Aegilops complex groups demonstrated high cross-affinity with each other to overcome the barriers of distant hybridization(Loureiro et al.,2023).Distant hybridization involves two distinct yet closely rel... The Triticum-Aegilops complex groups demonstrated high cross-affinity with each other to overcome the barriers of distant hybridization(Loureiro et al.,2023).Distant hybridization involves two distinct yet closely related events:hybridization and genome doubling.Previous studies have indicated that bursts of transposable elements(TEs)can occur as a consequence or concomitant to hybridization or genome duplication(Parisod et al.,2010).This raises an important scientific question regarding how the TEs-rich centromere region copes with genomic shock(McClintock,1984).The Triticum-Aegilops species complexes,particularly in the F1,So,and subsequent early generations resulting from successive selfcrossing,offer an opportunity to investigate whether the centromere environment undergoes reconstruction and the associated mechanisms that maintain genomic stability. 展开更多
关键词 centromere TRITICUM complex
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Non-B-form DNA is associated with centromere stability in newly-formed polyploid wheat
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作者 Congyang Yi Qian Liu +6 位作者 Yuhong Huang Chang Liu Xianrui Guo Chaolan Fan Kaibiao Zhang Yang Liu Fangpu Han 《Science China(Life Sciences)》 SCIE CAS CSCD 2024年第7期1479-1488,共10页
Non-B-form DNA differs from the classic B-DNA double helix structure and plays a crucial regulatory role in replication and transcription.However,the role of non-B-form DNA in centromeres,especially in polyploid wheat... Non-B-form DNA differs from the classic B-DNA double helix structure and plays a crucial regulatory role in replication and transcription.However,the role of non-B-form DNA in centromeres,especially in polyploid wheat,remains elusive.Here,we systematically analyzed seven non-B-form DNA motif profiles(A-phased DNA repeat,direct repeat,G-quadruplex,inverted repeat,mirror repeat,short tandem repeat,and Z-DNA)in hexaploid wheat.We found that three of these non-B-form DNA motifs were enriched at centromeric regions,especially at the CENH3-binding sites,suggesting that non-B-form DNA may create a favorable loading environment for the CENH3 nucleosome.To investigate the dynamics of centromeric non-B form DNA during the alloploidization process,we analyzed DNA secondary structure using CENH3 ChIP-seq data from newly formed allotetraploid wheat and its two diploid ancestors.We found that newly formed allotetraploid wheat formed more non-B-form DNA in centromeric regions compared with their parents,suggesting that non-B-form DNA is related to the localization of the centromeric regions in newly formed wheat.Furthermore,non-B-form DNA enriched in the centromeric regions was found to preferentially form on young LTR retrotransposons,explaining CENH3's tendency to bind to younger LTR.Collectively,our study describes the landscape of non-B-form DNA in the wheat genome,and sheds light on its potential role in the evolution of polyploid centromeres. 展开更多
关键词 non-B-form DNA polyploid wheat centromere RETROTRANSPOSON
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3种滨海碱蓬属植物串联重复序列与转座元件比较分析
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作者 陈静 黄永吉 《福建农业科技》 CAS 2024年第8期16-22,共7页
为了解析3种常见的滨海碱蓬属物种的重复序列类型、丰度、染色体分布模式及种间差异,采用基于序列相似性聚类分析和荧光原位杂交技术,对它们的重复序列进行比较分析。结果表明:3个物种中均存在串联重复序列和转座元件。其中,端粒序列、5... 为了解析3种常见的滨海碱蓬属物种的重复序列类型、丰度、染色体分布模式及种间差异,采用基于序列相似性聚类分析和荧光原位杂交技术,对它们的重复序列进行比较分析。结果表明:3个物种中均存在串联重复序列和转座元件。其中,端粒序列、5S rDNA、35S rDNA为3个物种保守的串联重复序列,另外有2个串联重复序列是碱蓬中特异的,这些串联重复序列倾向于在染色体端粒、亚端粒、染色体臂富集。此外,3个碱蓬属物种的转座元件主要由反转录转座子和DNA转座子组成,其中Ty3/gypsy反转录转座子的基因组占比较高,而Ty1/copia反转录转座子和DNA转座子的基因组含量则相对较低。除了CRM谱系主要在着丝粒区域聚集,大多数转座元件在染色体上呈现散布分布,而一些基因组丰度较低的转座元件则未检测到信号。根据重复序列和染色体分布分析结果表明,与碱蓬相比,南方碱蓬和盐地碱蓬的基因组关系更近。这些发现将为理解3种滨海碱蓬属物种重复序列的特征和种间进化关系提供了依据。 展开更多
关键词 碱蓬属 串联重复序列 端粒 着丝粒 RDNA 荧光原位杂交
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CENPF调控PI3K/AKT信号通路影响唾液腺腺样囊性癌进展
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作者 杨心怡 黄薇薇 +1 位作者 黄丽 胡赟 《口腔医学研究》 CAS CSCD 北大核心 2024年第9期803-809,共7页
目的:探讨着丝粒蛋白F(centromere protein F,CENPF)在腺样囊性癌(adenoid cystic carcinoma,ACC)发生发展中的作用和分子机制。方法:通过小干扰RNA转染敲低ACC细胞中的CENPF。通过细胞计数试剂盒(cell counting kit-8,CCK-8)、划痕实验... 目的:探讨着丝粒蛋白F(centromere protein F,CENPF)在腺样囊性癌(adenoid cystic carcinoma,ACC)发生发展中的作用和分子机制。方法:通过小干扰RNA转染敲低ACC细胞中的CENPF。通过细胞计数试剂盒(cell counting kit-8,CCK-8)、划痕实验和Transwell测定评估敲低CENPF对ACC细胞增殖、迁移、侵袭的影响;通过蛋白免疫印迹实验分析改变CENPF表达后ACC细胞中磷脂酰肌醇-3-激酶(phosphatidylinositol-3-kinase,PI3K)/丝氨酸/苏氨酸蛋白激酶(serine/threonine protein kinase,AKT)/哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)信号通路相关蛋白表达变化,通过CCK-8、划痕实验和Transwell测定评估敲低CENPF联合PI3K抑制剂BKM-120对ACC细胞增殖、迁移、侵袭的影响。结果:敲低ACC-M中CENPF表达后,CCK-8实验证明其增殖能力减弱;划痕实验表明其迁移能力减弱,Transwell实验表明其侵袭能力减弱,PI3K/AKT通路关键蛋白p-PI3K、p-AKT和p-mTOR蛋白表达水平下降。在下调CENPF的ACC-M中加入PI3K抑制剂,PI3K/AKT通路关键蛋白表达水平进一步下降。此外,加入PI3K抑制剂后,CENPF下调的ACC-M增殖、迁移、侵袭能力较单纯下调CENPF的ACC-M进一步减弱。结论:CENPF通过调控PI3K/AKT信号通路参与ACC进展。 展开更多
关键词 着丝粒蛋白F 腺样囊性癌 PI3K/AKT信号通路 机制研究
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