Susceptibility gene for stroke or cerebral infarction in the Han population in Hunan Province of China

The scavenger receptor class B type I gene can protect against atherosclerosis; a mononucleotide polymorphism is associated with differences in blood lipid metabolism, postprandial serum lipid levels, insulin resistance, coronary artery disease and familial hyperlipidemia. In this study, the scavenger receptor class B type I gene exon 1 G4A gene polymorphism in atherosclerotic cerebral infarction patients, cerebral hemorrhage patients and normal controls was detected using the polymerase chain reaction-restriction fragment length polymorphism method. The results showed that the GA ＋ AA genotype frequency of scavenger receptor class B type I gene G4A in atherosclerotic cerebral infarction patients was similar to that in cerebral hemorrhage patients and normal controls; however, the A allele frequency was significantly lower than that in normal controls. The serum level of high-density lipoprotein cholesterol in patients with the scavenger receptor class B type I gene G4A GA ＋ AA genotype was significantly higher, while the serum level of low-density lipoprotein cholesterol was significantly lower than that in patients with the GG genotype, in both the atherosclerotic cerebral infarction and cerebral hemorrhage groups. The serum level of high-density lipoprotein cholesterol in patients with the scavenger receptor class B type I gene G4A GA ＋ AA genotype was significantly higher, while the serum levels of low-density lipoprotein cholesterol and total cholesterol were significantly lower than those in normal controls with the GG genotype. Our experimental results suggest that the G4A polymorphism of the scavenger receptor class B type I gene is a possible predisposing risk factor for atherosclerotic cerebral infarction, and that it has no association with cerebral hemorrhage in the Hart population in Hunan province of China. The A allele is possibly associated with the metabolism of high-density and low-density lipoprotein cholesterol.

Tag single nucleotide polymorphism rs1532624 located in cholesteryl ester transfer protein gene is associated with atherosclerosis cerebral ischemia

Objective: To investigate the relationship between polymorphisms of rs1532624 and rs289741 loci in cholesteryl ester transfer protein(CETP) genes and atherosclerotic cerebral infarction(ACI). Methods: The CETP gene rs1532624 and rs289741 in 95 patients with ACI and 177 healthy subjects were genotyped by Mass ARRAY mass spectrometry. Each locus genotype and allele frequency distributions were compared. Results: The difference of allele frequency distribution between the rs1532624(χ~2=1.723, P=0.189) and rs289741(χ~2=2.466, P=0.116) were not statistically significant. The frequency distribution of rs1532624 genotype between the cerebral infarction group and healthy control group was statistically significant(χ~2=7.096, P=0.029), while rs289741 genotype frequency distribution between the two groups was not statistically significant(χ~2=2.906, P=0.234). Conclusion: ACI have a positive correlation with rs1532624 polymorphism, and AA genotype may be susceptible factors of ACI.

APOA5-1131T>C polymorphism is associated with atherosclerotic cerebral infarction in Han Chinese

Background Apolipoprotein （apo） A-V is a novel member of the apolipoprotein cluster involved in triacylglycerol （TG） homeostasis. It has reported that APOA5 gene polymorphisms is correlated with arteriosclerotic diseases. However, This association is unknown on Chinese patients with atherosclerotic cerebral infarction. The present study aimed to elucidate the relationship of APOA5 -1131T 〉 C and arteriosclerotic cerebral infarction （ACI） as well as the levels of serum lipids. Methods Polymerase chain reaction-restriction fragments length polymorphisms （PCR-RFLP） analysis, enzymatic and immunoturbidimetry methods were used to measure- 1131T 〉 C genotype, allele frequency as well as plasma lipid level of 90 ACI patients and 221 healthy subjects of Han Chinese. Results In ACI group, the level of TG in allele C carriers was higher than that of non-C carriers （P 〈 0.05）. The frequency of allele C in ACI group was higher than in healthy group （~2 = 5.568, P = 0.018）. Except sex, age and BMI, the levels of total cholesterol （TC）, triglycerides （TG）, high density lipoprotein choles- terol （HDL-C）, low-density lipoprotein cholesterol （LDL-C）, APOA1 and APOB in ACI group distinctively were higher than those in healthy group. Conclusion The APOA5-1131 allele C is associated with the high level of TG in ACI patients, which is probably linked with ACI danger of Chinese Han.