Neuronal quantity and ultrastructure in extracorticospinal tract of newborn rabbits with cerebral palsy

BACKGROUND:The liver enzyme system and blood brain barrier function of newborn rabbits are incomplete.High level of bilirubin can lead to cerebral palsy(CP)of newborn rabbits.The pathological and ultrastructural changes of brains of immature rabbits may differ from those of mature rabbits.OBJECTIVE:To observe the changes in dopaminergic neuron amount and ultrastructure in the extracorticospinal tract of animal models of CP induced by hyperbilirubinemia.DESIGN:A randomized controlled observation.SETTING:Rehabilitation Medical College of Jiamusi University;Laboratory for Pediatric Neurology Rehabilitation of Jiamusi University.MATERIALS:Thirty newborn 2-to 5-day inbred Japanese rabbits,weighing about 50 g,of either gender,were provided by Experimental Animal Center,Jilin University.Bilirubin and tyrosine hydroxylase(TH)antibody were purchased from Sigma Corporation,USA.METHODS:This experiment was carried out in the laboratory for children neuro-rehabilitation,Jiamusi University between September 2002 and December 2005.①Thirty rabbits were randomly divided into 2 groups:control group(n=10)and model group(n=20).②Bilirubin of 100 mg/kg was given to the rabbits of model group through intraperitoneal injection,once every other day,3 times totally;The same amount of normal saline was given to the rabbits of control group.After injection,the newborn rabbits had been fed by maternal milk for 45 days.③TH-positive neurons in substantia nigra and corpora striatum in each area of 0.15 mm×0.15 mm were counted.Ultrastructures of substantia nigra and corpora striatum were observed under the electron miscroscope.The concentration of bilirubin in brain tissue of CP rabbits of model group was measured with high performance liquid chromatogram.MAIN OUTCOME MEASURES:Quantitative analysis results and ultrastructure of TH-positive neurons in substantia nigra and corpora striatum in two groups.RESULTS:Thirty newborn rabbits were involved,6 rabbits from model group died and 4 were failed in modeling,finally,20 rabbits were involved in the result analysis.①The concentration of bilirubin in brain tissue of newborn rabbits of model group was(59.9±17.04)mg/L,which was significantly higher than that of control group[(42.79±3.57)mg/L,P<0.01].②TH positive neurons amount in the substantia nigra and corpora striatum of newborn rabbits in the model group was 30.30±9.58,4.70±2.79,which was significantly less than that in the control group respectively(45.20±12.74,11±2.45,P<0.01).③The concentration of bilirubin in brain tissue was significantly negatively correlated with TH-positive neurons in substantia nigra(r=-0.69,P<0.01);The concentration of bilirubin in brain tissue was also significantly negatively correlated with TH-positive neurons in corpora striatum(r=-0.59,P<0.01).TH-positive neurons in substantia nigra were positively correlated with those in corpora striatum(r=0.77,P<0.01).④Under the electron microscope,the nerve cells in substantia nigra and corpora striatum of newborn rabbits of model group were significantly changed in ultrastructure as compared with control group.CONCLUSION:Ultrastructure of extracorticospinal tract of newborn rabbits with CP is significantly changed,presenting the injury of dopaminergic neuron and decrease of neuronal amount.

EFFECT OF ELECTROACUPUNCTURE ON SYNAPTIC PLASTICITY OF HIPPOCAMPAL NEURONS IN CEREBRAL ISCHEMIA RATS

Objective: To observe the effect of electroacupuncture (EA) on synaptic structure of hippocampal nerve felts and synaptophysin(SYN)expression in rats with cerebral ischemic injury. Methods: Sixty Wistar rats were randomized into sham-operation group, cerebral ischemia (CI) group and EA group, each of which was further divided into 1week (W) and 5W subgroups. CI injury model was established by occlusion of the bilateral common carotid arteries. 'Baihui'(百会 GV 20), 'Dazhui' (大椎 GV 14), 'Renzhong'(人中 GV 26) and 'Guangyuan'(关会 CV 4) were punctured and stimulated electrically. The brain tissue sections containing hippocampus region were stained with immu nohistochemical technique and observed under light microscope and transmission electronic microscope. Results: After CI, the ischemic injury as degeneration of the presynapse compositions, decrease of the synaptic numeral density, and low expression of SYN were observed in hippocampal CA1 area. By the 5th week after CI, the neonatal synapses of Cl and EA groups appeared, and SYN expression was upregulated. In EA group, the recovery of the numeral density of synapses was especially noticeable, being 93.8% of that of sham-operation group and significantly higher than that in Cl group (P<0.01). Compared with sham-operation group, the calibrated optical density (COD) values of SYN increased to 70% in CI group, and 93.3% in EA group, and COD value in EA group was significantly higher than that in Cl group (P<0.01). Conclusion: EA can function in promoting synaptic regeneration and enhancing and perfecting the actions of the reconstructed synapses in hippocampal CA1 area in Cl rats.