During the previous years, with the emerging of nanotechnology, the enormous capabilities of nanoparticles have drawn great attention from researchers in terms of their potentials in various aspects of pharmacology. C...During the previous years, with the emerging of nanotechnology, the enormous capabilities of nanoparticles have drawn great attention from researchers in terms of their potentials in various aspects of pharmacology. Cerium oxide nanoparticles(nanoceria), considered as one of the most widely used nanomaterials, due to its tempting catalytic antioxidant properties, show a promising potential in diverse disorders, such as cerebral ischemic stroke(CIS), cancer, neurodegenerative and inflammatory diseases. Overwhelming generation of reactive oxygen species(ROS) and reactive nitrogen species(RNS) during cerebral ischemia and reperfusion periods is known to aggravate brain damage via sophisticated cellular and molecular mechanisms, and therefore exploration of the antioxidant capacities of nanoceria becomes a new approach in reducing cerebral ischemic injury. Furthermore, utilizing nanoceria as a drug carrier might display the propensity to overcome limitations or inefficacy of other conceivable neuroprotectants and exhibit synergistic effects. In this review, we emphasize on the principle features of nanoceria and current researches concerning nanoceria as a potential therapeutic agent or carrier in improving the prognosis of CIS.展开更多
Cerium oxide nanoparticles(CNPs)possess a great potential as therapeutic agents due to their ability to self-regenerate by reversibly switching between two valences+3 and+4.This article reviews recent articles dealing...Cerium oxide nanoparticles(CNPs)possess a great potential as therapeutic agents due to their ability to self-regenerate by reversibly switching between two valences+3 and+4.This article reviews recent articles dealing with in vivo studies of CNPs towards Alzheimer’s disease,obesity,liver inflammation,cancer,sepsis,amyotrophic lateral sclerosis,acute kidney injury,radiation-induced tissue damage,hepatic ischemia reperfusion injury,retinal diseases and constipation.In vivo anti-cancer studies revealed the effectiveness of CNPs to reduce tumor growth and angiogenesis in melanoma,ovarian,breast and retinoblastoma cancer cell-induced mice,with their conjugation with folic acid,doxorubicin,CPM,or CXC receptor-4 antagonist ligand eliciting higher efficiency.After conjugation with triphenylphosphonium or magnetite nanoparticles,CNPs were shown to combat Alzheimer’s disease by reducing amyloid-β,glial fibrillary acidic protein,inflammatory and oxidative stress markers in mice.By improving muscle function and longevity,the citrate/EDTA-stabilized CNPs could ameliorate amyotrophic lateral sclerosis.Also,they could effectively reduce obesity in mice by scavenging ROS and reducing adipogenesis,triglyceride synthesis,GAPDH enzyme activity,leptin and insulin levels.In CCl4-induced rats,stress signaling pathways due to inflammatory cytokines,liver enzymes,oxidative and endoplasmic reticulum messengers could be attenuated by CNPs.Commercial CNPs showed protective effects on rats with hepatic ischemia reperfusion and peritonitis-induced hepatic/cardiac injuries by decreasing oxidative stress and hepatic/cardiac inflammation.The same CNPs could improve kidney function by diminishing renal superoxide,hyperglycemia and tubular damage in peritonitis-induced acute kidney injury in rats.Radiation-induced lung and testicular tissue damage could be alleviated in mice,with the former showing improvement in pulmonary distress and bronchoconstriction and the latter exhibiting restoration in spermatogenesis rate and spermatid/spermatocyte number.Through enhancement of gastrointestinal motility,the CNPs could alleviate constipation in both young and old rats.They could also protect rat from light-induced retinal damage by slowing down neurodegenerative process and microglial activation.展开更多
Metal-and metal-oxide-based nanoparticles have been widely exploited in cancer photodynamic therapy(PDT).Among these materials,cerium-based nanoparticles have drawn extensive attention due to their superior biosafety ...Metal-and metal-oxide-based nanoparticles have been widely exploited in cancer photodynamic therapy(PDT).Among these materials,cerium-based nanoparticles have drawn extensive attention due to their superior biosafety and distinctive physicochemical properties,especially the reversible transition between the valence states of Ce(Ⅲ)and Ce(Ⅳ).In this review,the recent advances in the use of cerium-based nanoparticles as novel photosensitizers for cancer PDT are discussed,and the activation mechanisms for electron transfer to generate singlet oxygen are presented.In addition,the types of cerium-based nanoparticles used for PDT of cancer are summarized.Finally,the challenges and prospects of clinical translations of cerium-based nanoparticles are briefly addressed.展开更多
We examined the possibility of developing a new risk assessment method for potentially hazardous chemicals by using mouse primary hepatocytes from acatalasemic mice (Csb) and the wild-type (Csa) as predictive model. C...We examined the possibility of developing a new risk assessment method for potentially hazardous chemicals by using mouse primary hepatocytes from acatalasemic mice (Csb) and the wild-type (Csa) as predictive model. Chemical-induced cytotoxicities, such as hydrogen peroxide and lawsone, a main hair dye ingredient of henna, were examined. We observed the differences in cell survival between the Csa and Csb in a dose-dependant manner after treatment with either hydrogen peroxide or lawsone, supporting the usefulness of this newly established method for hazard identification of oxidative chemicals in a risk assessment process. More chemicals will be tested to confirm the usefulness of this method for the preliminary screening of oxidative chemicals before animal experimentation.展开更多
Respiratory syncytial virus(RSV)is the most common cause of viral bronchiolitis among children worldwide,yet there is no vaccine for RSV disease.This study investigates the potential of cube and sphere-shaped cerium o...Respiratory syncytial virus(RSV)is the most common cause of viral bronchiolitis among children worldwide,yet there is no vaccine for RSV disease.This study investigates the potential of cube and sphere-shaped cerium oxide nanoparticles(CNP)to modulate reactive oxygen(ROS)and nitrogen(RNS)species and immune cell phenotypes in the presence of RSV infection in vitro and in vivo.Cube and sphere-shaped CNP were synthesized by hydrothermal and ultrasonication methods,respectively.Physico-chemical characterization confirmed the shape of sphere and cube CNP and effect of various parameters on their particle size distribution and zeta potential.In vitro results revealed that sphere and cube CNP differentially modulated ROS and RNS levels in J774 macrophages.Specifically,cube CNP significantly reduced RSV-induced ROS levels without affecting RNS levels while sphere CNP increased RSV-induced RNS levels with minimal effect on ROS levels.Cube CNP drove an M1 phenotype in RSV-infected macrophages in vitro by increasing macrophage surface expression of CD80 and CD86 with a concomitant increase in TNFαand IL-12p70,while simultaneously decreasing M2 CD206 expression.Intranasal administration of sphere and cube-CNP were well-tolerated with no observed toxicity in BALB/c mice.Notably,cube CNP preferentially accumulated in murine alveolar macrophages and induced their activation,avoiding enhanced uptake and activation of other inflammatory cells such as neutrophils,which are associated with RSV-mediated inflammation.In conclusion,we report that sphere and cube CNP modulate macrophage polarization and innate cellular responses during RSV infection.展开更多
Reactive oxygen species(ROS)plays important roles in living organisms.While ROS is a double-edged sword,which can eliminate drug-resistant bacteria,but excessive levels can cause oxidative damage to cells.A core–shel...Reactive oxygen species(ROS)plays important roles in living organisms.While ROS is a double-edged sword,which can eliminate drug-resistant bacteria,but excessive levels can cause oxidative damage to cells.A core–shell nanozyme,Ce O_(2)@ZIF-8/Au,has been crafted,spontaneously activating both ROS generating and scavenging functions,achieving the multifaceted functions of eliminating bacteria,reducing inflammation,and promoting wound healing.The Au Nanoparticles(NPs)on the shell exhibit high-efficiency peroxidase-like activity,producing ROS to kill bacteria.Meanwhile,the encapsulation of Ce O_(2) core within ZIF-8 provides a seal for temporarily limiting the superoxide dismutase and catalase-like activities of Ce O_(2) nanoparticles.Subsequently,as the ZIF-8 structure decomposes in the acidic microenvironment,the Ce O_(2) core is gradually released,exerting its ROS scavenging activity to eliminate excess ROS produced by the Au NPs.These two functions automatically and continuously regulate the balance of ROS levels,ultimately achieving the function of killing bacteria,reducing inflammation,and promoting wound healing.Such innovative ROS spontaneous regulators hold immense potential for revolutionizing the field of antibacterial agents and therapies.展开更多
Dry eye disease(DED)is the most common eye disease in ophthalmic consultation except for refractive errors.Therefore,an exploration of valid and alternative therapeutic interventions is essential to feed the urgent me...Dry eye disease(DED)is the most common eye disease in ophthalmic consultation except for refractive errors.Therefore,an exploration of valid and alternative therapeutic interventions is essential to feed the urgent medical need.It has been demonstrated that oxidative stress causes multiple adverse effects in the pathogenesis of DED,thence alleviating oxidative stress is an effective therapeutic strategy for the DED treatment.Herein,we developed a cerium oxide nanozyme combined with branched poly(ethylene imine)-graftpoly(ethylene glycol)(bPEI-g-PEG).Owing to its stable hydrophilic chains on the surface reducing the cytotoxicity and loads of amines groups that be combined with cerium ions through coordination bonds,the modified nanozymes(referred to as CNP@bPEI-g-PEG)are water soluble and highly biocompatible.Meanwhile,due to its excellent antioxidant activity,CNP@bPEI-g-PEG nanozymes can mimic the activity of superoxide dismutase and catalase to scavenge intracellular reactive oxygen species(ROS).Experimental studies firmly demonstrated that the modified nanozymes were auto-regenerative and more active in scavenging excessive ROS and alleviating oxidative stress by cerium-element valence state recycling,recovering the morphology of corneal,conjunctival epithelium and the number of goblet cells.The advanced combination may offer a superior therapeutic strategy to deal with oxidative stress for effective treatment of DED.展开更多
Objective: To investigate the effect of ginsenosides from stems and leaves of ginseng on ethanolinduced lipid deposition in human LO2 hepatocytes. Methods: LO2 cells were exposed to ethanol for 36 h and treated with...Objective: To investigate the effect of ginsenosides from stems and leaves of ginseng on ethanolinduced lipid deposition in human LO2 hepatocytes. Methods: LO2 cells were exposed to ethanol for 36 h and treated with or without ginsenosides. The viability of LO2 cells was evaluated by methylthiazolyldiphenyl- tetrazolium bromide assay and the triglyceride (TG) content was detected. Lipid droplets were determined by oil red O staining. Intracellular reactive oxygen species (ROS) production and the mitochondrial membrane potential were tested by flow cytometry. The ATP level was measured by reverse phase high performance liquid chromatography. The expression of cytochrome p450 2E1 (CYP2E1) and peroxisome proliferator- activated receptor (PPAR α) was detected by reverse transcriptase-polymerase chain reaction and Western blotting, respectively. Results: Ethanol exposure resulted in the increase of TG level, lipid accumulation and ROS generation, and the decrease of mitochondrial membrane potential and ATP production in the cells. However, ginsenosides significantly reduced TG content (9.69 ± 0.22 μg/mg protein vs. 4.93 ± 0.49 μg/mg protein, P〈0.01), and ROS formation (7254.8± 385.7 vs. 5825.2± 375.9, P〈0.01). Meanwhile, improvements in mitochondrial membrane potential (10655.33± 331.34 vs. 11129.52 ± 262.35, P〈0.05) and ATP level (1.20±0.18 nmol/mg protein vs. 2.53±0.25 nmol/mg protein, P〈0.01) were observed by treatment with ginsenosides. Furthermore, ginsenosides could down-regulate CYP2E1 expression (P〈0.01) and upregulate PPAR α expression (P〈0.01) in ethanol-treated cells. Conclusions: Ginsenosides could prevent ethanol-induced hepatocyte steatosis in vitro related to the inhibition of oxidative stress and the improvement of mitochondrial function. In addition, the modulation of CYP2E1 and PPAR α expression may also play an important role in the protective effect of ginsenosides against lipid accumulation.展开更多
文摘During the previous years, with the emerging of nanotechnology, the enormous capabilities of nanoparticles have drawn great attention from researchers in terms of their potentials in various aspects of pharmacology. Cerium oxide nanoparticles(nanoceria), considered as one of the most widely used nanomaterials, due to its tempting catalytic antioxidant properties, show a promising potential in diverse disorders, such as cerebral ischemic stroke(CIS), cancer, neurodegenerative and inflammatory diseases. Overwhelming generation of reactive oxygen species(ROS) and reactive nitrogen species(RNS) during cerebral ischemia and reperfusion periods is known to aggravate brain damage via sophisticated cellular and molecular mechanisms, and therefore exploration of the antioxidant capacities of nanoceria becomes a new approach in reducing cerebral ischemic injury. Furthermore, utilizing nanoceria as a drug carrier might display the propensity to overcome limitations or inefficacy of other conceivable neuroprotectants and exhibit synergistic effects. In this review, we emphasize on the principle features of nanoceria and current researches concerning nanoceria as a potential therapeutic agent or carrier in improving the prognosis of CIS.
文摘Cerium oxide nanoparticles(CNPs)possess a great potential as therapeutic agents due to their ability to self-regenerate by reversibly switching between two valences+3 and+4.This article reviews recent articles dealing with in vivo studies of CNPs towards Alzheimer’s disease,obesity,liver inflammation,cancer,sepsis,amyotrophic lateral sclerosis,acute kidney injury,radiation-induced tissue damage,hepatic ischemia reperfusion injury,retinal diseases and constipation.In vivo anti-cancer studies revealed the effectiveness of CNPs to reduce tumor growth and angiogenesis in melanoma,ovarian,breast and retinoblastoma cancer cell-induced mice,with their conjugation with folic acid,doxorubicin,CPM,or CXC receptor-4 antagonist ligand eliciting higher efficiency.After conjugation with triphenylphosphonium or magnetite nanoparticles,CNPs were shown to combat Alzheimer’s disease by reducing amyloid-β,glial fibrillary acidic protein,inflammatory and oxidative stress markers in mice.By improving muscle function and longevity,the citrate/EDTA-stabilized CNPs could ameliorate amyotrophic lateral sclerosis.Also,they could effectively reduce obesity in mice by scavenging ROS and reducing adipogenesis,triglyceride synthesis,GAPDH enzyme activity,leptin and insulin levels.In CCl4-induced rats,stress signaling pathways due to inflammatory cytokines,liver enzymes,oxidative and endoplasmic reticulum messengers could be attenuated by CNPs.Commercial CNPs showed protective effects on rats with hepatic ischemia reperfusion and peritonitis-induced hepatic/cardiac injuries by decreasing oxidative stress and hepatic/cardiac inflammation.The same CNPs could improve kidney function by diminishing renal superoxide,hyperglycemia and tubular damage in peritonitis-induced acute kidney injury in rats.Radiation-induced lung and testicular tissue damage could be alleviated in mice,with the former showing improvement in pulmonary distress and bronchoconstriction and the latter exhibiting restoration in spermatogenesis rate and spermatid/spermatocyte number.Through enhancement of gastrointestinal motility,the CNPs could alleviate constipation in both young and old rats.They could also protect rat from light-induced retinal damage by slowing down neurodegenerative process and microglial activation.
基金supported by the National Natural Science Foundation(NNSF)of China(52103166 and 61935004)the Natural Science Foundation(NSF)of Jiangsu Province(BK20200710)+3 种基金Jiangsu Postdoctoral Science Foundation(51204087)NSF of Shandong Province(ZR2020KB018)Taishan Scholars"Construction Special Fund of Shandong Province,the Natural Science Foundation of Ningbo(202003N40448)the Open Project Program of Wuhan National Laboratory for Optoelectronics No.2020WNLOKF022.
文摘Metal-and metal-oxide-based nanoparticles have been widely exploited in cancer photodynamic therapy(PDT).Among these materials,cerium-based nanoparticles have drawn extensive attention due to their superior biosafety and distinctive physicochemical properties,especially the reversible transition between the valence states of Ce(Ⅲ)and Ce(Ⅳ).In this review,the recent advances in the use of cerium-based nanoparticles as novel photosensitizers for cancer PDT are discussed,and the activation mechanisms for electron transfer to generate singlet oxygen are presented.In addition,the types of cerium-based nanoparticles used for PDT of cancer are summarized.Finally,the challenges and prospects of clinical translations of cerium-based nanoparticles are briefly addressed.
文摘We examined the possibility of developing a new risk assessment method for potentially hazardous chemicals by using mouse primary hepatocytes from acatalasemic mice (Csb) and the wild-type (Csa) as predictive model. Chemical-induced cytotoxicities, such as hydrogen peroxide and lawsone, a main hair dye ingredient of henna, were examined. We observed the differences in cell survival between the Csa and Csb in a dose-dependant manner after treatment with either hydrogen peroxide or lawsone, supporting the usefulness of this newly established method for hazard identification of oxidative chemicals in a risk assessment process. More chemicals will be tested to confirm the usefulness of this method for the preliminary screening of oxidative chemicals before animal experimentation.
基金funding support from the School of Pharmacy,University of Pittsburgh(SS),Brenneman Fund,University of Pittsburgh(KME)Graduate Student Research Scholarship from the School of Pharmacy,University of Pittsburgh(AP)special BD LSRFORTESSATM funded by NIH 1S10OD011925-01(PI:Borghesi).
文摘Respiratory syncytial virus(RSV)is the most common cause of viral bronchiolitis among children worldwide,yet there is no vaccine for RSV disease.This study investigates the potential of cube and sphere-shaped cerium oxide nanoparticles(CNP)to modulate reactive oxygen(ROS)and nitrogen(RNS)species and immune cell phenotypes in the presence of RSV infection in vitro and in vivo.Cube and sphere-shaped CNP were synthesized by hydrothermal and ultrasonication methods,respectively.Physico-chemical characterization confirmed the shape of sphere and cube CNP and effect of various parameters on their particle size distribution and zeta potential.In vitro results revealed that sphere and cube CNP differentially modulated ROS and RNS levels in J774 macrophages.Specifically,cube CNP significantly reduced RSV-induced ROS levels without affecting RNS levels while sphere CNP increased RSV-induced RNS levels with minimal effect on ROS levels.Cube CNP drove an M1 phenotype in RSV-infected macrophages in vitro by increasing macrophage surface expression of CD80 and CD86 with a concomitant increase in TNFαand IL-12p70,while simultaneously decreasing M2 CD206 expression.Intranasal administration of sphere and cube-CNP were well-tolerated with no observed toxicity in BALB/c mice.Notably,cube CNP preferentially accumulated in murine alveolar macrophages and induced their activation,avoiding enhanced uptake and activation of other inflammatory cells such as neutrophils,which are associated with RSV-mediated inflammation.In conclusion,we report that sphere and cube CNP modulate macrophage polarization and innate cellular responses during RSV infection.
基金supported by the Natural Science Foundation of Fujian Province of China(No.2022J01043)China Scholarship Council(201806315005 and 201703170071).
文摘Reactive oxygen species(ROS)plays important roles in living organisms.While ROS is a double-edged sword,which can eliminate drug-resistant bacteria,but excessive levels can cause oxidative damage to cells.A core–shell nanozyme,Ce O_(2)@ZIF-8/Au,has been crafted,spontaneously activating both ROS generating and scavenging functions,achieving the multifaceted functions of eliminating bacteria,reducing inflammation,and promoting wound healing.The Au Nanoparticles(NPs)on the shell exhibit high-efficiency peroxidase-like activity,producing ROS to kill bacteria.Meanwhile,the encapsulation of Ce O_(2) core within ZIF-8 provides a seal for temporarily limiting the superoxide dismutase and catalase-like activities of Ce O_(2) nanoparticles.Subsequently,as the ZIF-8 structure decomposes in the acidic microenvironment,the Ce O_(2) core is gradually released,exerting its ROS scavenging activity to eliminate excess ROS produced by the Au NPs.These two functions automatically and continuously regulate the balance of ROS levels,ultimately achieving the function of killing bacteria,reducing inflammation,and promoting wound healing.Such innovative ROS spontaneous regulators hold immense potential for revolutionizing the field of antibacterial agents and therapies.
基金financially supported by the Key Scientific and Technological Innovation Projects in Wenzhou[ZY2021002]the Medical&Health Technology Program of Zhejiang Province[2022RC051].
文摘Dry eye disease(DED)is the most common eye disease in ophthalmic consultation except for refractive errors.Therefore,an exploration of valid and alternative therapeutic interventions is essential to feed the urgent medical need.It has been demonstrated that oxidative stress causes multiple adverse effects in the pathogenesis of DED,thence alleviating oxidative stress is an effective therapeutic strategy for the DED treatment.Herein,we developed a cerium oxide nanozyme combined with branched poly(ethylene imine)-graftpoly(ethylene glycol)(bPEI-g-PEG).Owing to its stable hydrophilic chains on the surface reducing the cytotoxicity and loads of amines groups that be combined with cerium ions through coordination bonds,the modified nanozymes(referred to as CNP@bPEI-g-PEG)are water soluble and highly biocompatible.Meanwhile,due to its excellent antioxidant activity,CNP@bPEI-g-PEG nanozymes can mimic the activity of superoxide dismutase and catalase to scavenge intracellular reactive oxygen species(ROS).Experimental studies firmly demonstrated that the modified nanozymes were auto-regenerative and more active in scavenging excessive ROS and alleviating oxidative stress by cerium-element valence state recycling,recovering the morphology of corneal,conjunctival epithelium and the number of goblet cells.The advanced combination may offer a superior therapeutic strategy to deal with oxidative stress for effective treatment of DED.
基金Supported by Natural Science Foundation of Guangdong Province,China(No.S2012010008161)
文摘Objective: To investigate the effect of ginsenosides from stems and leaves of ginseng on ethanolinduced lipid deposition in human LO2 hepatocytes. Methods: LO2 cells were exposed to ethanol for 36 h and treated with or without ginsenosides. The viability of LO2 cells was evaluated by methylthiazolyldiphenyl- tetrazolium bromide assay and the triglyceride (TG) content was detected. Lipid droplets were determined by oil red O staining. Intracellular reactive oxygen species (ROS) production and the mitochondrial membrane potential were tested by flow cytometry. The ATP level was measured by reverse phase high performance liquid chromatography. The expression of cytochrome p450 2E1 (CYP2E1) and peroxisome proliferator- activated receptor (PPAR α) was detected by reverse transcriptase-polymerase chain reaction and Western blotting, respectively. Results: Ethanol exposure resulted in the increase of TG level, lipid accumulation and ROS generation, and the decrease of mitochondrial membrane potential and ATP production in the cells. However, ginsenosides significantly reduced TG content (9.69 ± 0.22 μg/mg protein vs. 4.93 ± 0.49 μg/mg protein, P〈0.01), and ROS formation (7254.8± 385.7 vs. 5825.2± 375.9, P〈0.01). Meanwhile, improvements in mitochondrial membrane potential (10655.33± 331.34 vs. 11129.52 ± 262.35, P〈0.05) and ATP level (1.20±0.18 nmol/mg protein vs. 2.53±0.25 nmol/mg protein, P〈0.01) were observed by treatment with ginsenosides. Furthermore, ginsenosides could down-regulate CYP2E1 expression (P〈0.01) and upregulate PPAR α expression (P〈0.01) in ethanol-treated cells. Conclusions: Ginsenosides could prevent ethanol-induced hepatocyte steatosis in vitro related to the inhibition of oxidative stress and the improvement of mitochondrial function. In addition, the modulation of CYP2E1 and PPAR α expression may also play an important role in the protective effect of ginsenosides against lipid accumulation.