Constituents from Aerial Parts of Bidens ceruna L. and Their DPPH Radical Scavenging Activity

A new chalcone glycoside, butein-4-methoxyl-4′-O-（6＂-O-acetyl）-β-D-glucopyranoside（2）, along with seven known compounds, namely, quercitrin（1）, luteolin-7-O-β-D-glucopyranoside（3）, butein-4＇-O-β-D-glucopyranoside（4）, butein-4-methoxyl-4＇-O-β-D-glucopyranoside（5）, butin-7-O-β-D-glucopyranoside（6）, isoquercitrin（7）, and sulfurein（8） was isolated from aerial parts of Bidens ceruna L. Their structures were elucidated on the basis of spectroscopic studies. Compounds 1-8 were tested for their DPPH radical scavenging activity and, except compounds 2 and 5, other compounds showed scavenging activitv.

RP-HPLC法测定柳叶鬼针草地上部分中3种黄酮苷的含量

目的:对柳叶鬼针草地上部分中的3种主要黄酮苷类成分木犀草素-7-O-β-D-吡喃葡萄糖苷(化合物Ⅰ)、槲皮苷(化合物Ⅱ)、紫铆因-4-甲氧基-4'-O-β-D-吡喃葡萄糖苷(化合物Ⅲ)进行含量测定。方法:采用反相高效液相色谱法,使用ZORBAX SB-C18(250mm×4.6mm,5μm)色谱柱,以乙腈-0.1%醋酸水溶液为流动相进行梯度洗脱(0～30min,16∶84;30～46min,24∶76),流速1.0mL·min-1,检测波长350nm,柱温25℃。结果:化合物Ⅰ～Ⅲ进样量在0.01～5.00μg范围内均有良好的线性关系(r≥0.9992,n=8)。化合物Ⅰ～Ⅲ的平均回收率(n=6)分别为100.3%(RSD为2.0%)、100.4%(RSD为1.8%)和102.6%(RSD为1.9%)。结论:本方法能够准确测定柳叶鬼针草中3种主要黄酮苷类成分含量。