Oral rinses containing chemotherapeutic agents, such as cetylpyridinium chloride （CPC）, can alleviate plaque-induced gingival infections, but how oral microbiota respond to these treatments in human population remai...Oral rinses containing chemotherapeutic agents, such as cetylpyridinium chloride （CPC）, can alleviate plaque-induced gingival infections, but how oral microbiota respond to these treatments in human population remains poorly understood. Via a double- blinded, randomised controlled trial of 91 subjects, the impact of CPC-containing oral rinses on supragingival plaque was investigated in experimental gingivitis, where the subjects, after a 21-day period of dental prophylaxis to achieve healthy gingivae, received either CPC rinses or water for 21 days. Within-subject temporal dynamics of plaque microbiota and symptoms of gingivitis were profiled via 16S ribosomal DNA gene pyrosequencing and assessment with the Mazza gingival index. Cetylpyridinium chloride conferred gingival benefits, as progression of gingival inflammation resulting from a lack of dental hygiene was significantly slower in the mouth rinse group than in the water group due to inhibition of 17 gingivitis-enriched bacterial genera. Tracking of plaque a and β diversity revealed that CPC treatment prevents acquisition of new taxa that would otherwise accumulate but maintains the original biodiversity of healthy plaques. Furthermore, CPC rinses reduced the size, local connectivity and microbiota-wide connectivity of the bacterial correlation network, particularly for nodes representing gingivitis- enriched taxa. The findings of this study provide mechanistic insights into the impact of oral rinses on the progression and maturation of dental plaque in the natural human population.展开更多
Natural zeolite was modified by loading cetylpyridinium bromide （CPB） to create more efficient sites for humic acid （HA） adsorption. The natural and CPB modified zeolites were characterized with X-ray diffraction,...Natural zeolite was modified by loading cetylpyridinium bromide （CPB） to create more efficient sites for humic acid （HA） adsorption. The natural and CPB modified zeolites were characterized with X-ray diffraction, field emission scanning electron microscopy, Fourier transform infrared spectroscopy and elemental analysis. The effects of various experimental parameters such as contact time, initial HA concentration, solution pH and coexistent Ca^2＋, upon HA adsorption onto CPB modified zeolites were evaluated. The results showed that natural zeolite had negligible affinity for HA in aqueous solutions, but CPB modified zeolites exhibited high adsorption efficiency for HA. A higher CPB loading on natural zeolites exhibited a larger HA adsorption capacity. Acidic pH and coexistent Ca^2＋ were proved to be favorable for HA adsorption onto CPB modified zeolite. The kinetic process was well described by pseudo second-order model. The experimental isotherm data fitted well to Langmuir and Sips models. The maximum monolayer adsorption capacity of CPB modified zeolite with surfactant bilayer coverage was found to be 92.0 mg/g.展开更多
There were already reports on using chromazol KS(CALKS) [trisodium salt of 2-(2-hydroxy-3-carboxy-5-sulphobenzolazo)-1, 8-dihydroxynaphthalene-3, 6-disulphonic acid] as a new reagent for the spectrophotometric determi...There were already reports on using chromazol KS(CALKS) [trisodium salt of 2-(2-hydroxy-3-carboxy-5-sulphobenzolazo)-1, 8-dihydroxynaphthalene-3, 6-disulphonic acid] as a new reagent for the spectrophotometric determination of aluminium. We also studied the ternary complex of aluminium(Ⅲ), iron(Ⅲ) gallium(Ⅲ) and beryllium (Ⅱ) with chromazol KS and cetylpyridinium bromide (CPB), and developed high sensitivity spectrophotometric methods for determining these elements.展开更多
基金funded by a joint research program between the Chinese Academy of Sciences and Procter & Gamble
文摘Oral rinses containing chemotherapeutic agents, such as cetylpyridinium chloride （CPC）, can alleviate plaque-induced gingival infections, but how oral microbiota respond to these treatments in human population remains poorly understood. Via a double- blinded, randomised controlled trial of 91 subjects, the impact of CPC-containing oral rinses on supragingival plaque was investigated in experimental gingivitis, where the subjects, after a 21-day period of dental prophylaxis to achieve healthy gingivae, received either CPC rinses or water for 21 days. Within-subject temporal dynamics of plaque microbiota and symptoms of gingivitis were profiled via 16S ribosomal DNA gene pyrosequencing and assessment with the Mazza gingival index. Cetylpyridinium chloride conferred gingival benefits, as progression of gingival inflammation resulting from a lack of dental hygiene was significantly slower in the mouth rinse group than in the water group due to inhibition of 17 gingivitis-enriched bacterial genera. Tracking of plaque a and β diversity revealed that CPC treatment prevents acquisition of new taxa that would otherwise accumulate but maintains the original biodiversity of healthy plaques. Furthermore, CPC rinses reduced the size, local connectivity and microbiota-wide connectivity of the bacterial correlation network, particularly for nodes representing gingivitis- enriched taxa. The findings of this study provide mechanistic insights into the impact of oral rinses on the progression and maturation of dental plaque in the natural human population.
基金supported by the National Major Project of Science & Technology Ministry of China (No.2008ZX07421-002)the National Natural Science Foundation of China (No. 50908142)
文摘Natural zeolite was modified by loading cetylpyridinium bromide （CPB） to create more efficient sites for humic acid （HA） adsorption. The natural and CPB modified zeolites were characterized with X-ray diffraction, field emission scanning electron microscopy, Fourier transform infrared spectroscopy and elemental analysis. The effects of various experimental parameters such as contact time, initial HA concentration, solution pH and coexistent Ca^2＋, upon HA adsorption onto CPB modified zeolites were evaluated. The results showed that natural zeolite had negligible affinity for HA in aqueous solutions, but CPB modified zeolites exhibited high adsorption efficiency for HA. A higher CPB loading on natural zeolites exhibited a larger HA adsorption capacity. Acidic pH and coexistent Ca^2＋ were proved to be favorable for HA adsorption onto CPB modified zeolite. The kinetic process was well described by pseudo second-order model. The experimental isotherm data fitted well to Langmuir and Sips models. The maximum monolayer adsorption capacity of CPB modified zeolite with surfactant bilayer coverage was found to be 92.0 mg/g.
文摘There were already reports on using chromazol KS(CALKS) [trisodium salt of 2-(2-hydroxy-3-carboxy-5-sulphobenzolazo)-1, 8-dihydroxynaphthalene-3, 6-disulphonic acid] as a new reagent for the spectrophotometric determination of aluminium. We also studied the ternary complex of aluminium(Ⅲ), iron(Ⅲ) gallium(Ⅲ) and beryllium (Ⅱ) with chromazol KS and cetylpyridinium bromide (CPB), and developed high sensitivity spectrophotometric methods for determining these elements.
