Qualitative and quantitative analysis of Chinese herb fructus chaenomelis

To establish reliable methods for evaluating the quality of Chinese herb fructus chaenomelis. Methods： Qualitative analysis by Thin layer chromatography （TLC） , reference substances were Chaenomeles speciosa （Sweet） Nakai and oleanolic acid, a mixed solvent of chloroform-methanol （40 ： 1 ） was employed as the mobile phase,color developing agent was 10% sulfuric acidethanol solution.In the system of high performance liquid chromatography （HPLC）, a Prontosil Eurobond C18 column （250 mm×4.0 mm,5μm ） was used,the mobile phase was composed of acetonitrile-methanol-0.4% ammonium acetate solution （55 ： 25 ： 20）, the flow rate was 1.0 ml/min with UV detected at 210 nm, the column temperature was maintained at room temperature. Results： In the system of TLC, oleanolic acid was separated successfully. In HPLC, the linear ranges of oleanolic acid and ursolic acid were 5.89-13.73 μg （R=0.9990）and 6.84-15.96μg （R=0.9990）, respectively. The average recoveries of oleanolic acid and ursolic acid were 97.52% （RSD=2 .58% ）, 98.21% （RSD=2.23%）, respectively. Conclusion： The established TLC method can easily distinguish Chinese herb fructus chaenomelis from other commonly used crude drugs of the same family .The HPLC method for determining oleanolic acid and ursolic acid is simple, reproducible, accurate and feasible. The methods reported in this paper can be used scientifically and effectively to evaluate the quality of Chinese herb fructus chaenomelis.

资木瓜多糖的提取和含量测定

[目的]建立资木瓜多糖的提取和含量测定方法。[方法]采用乙醇回流提取法提取资木瓜中的多糖,并利用苯酚-硫酸法测定多糖的含量。[结果]线性回归方程为:Y=0.008 8X-0.010 9(R2=0.992 3)。试验结果表明,在1.0～5.0μg/ml范围内,葡萄糖浓度与吸光度的线性关系良好。提取2次后,脱脂木瓜中多糖的含量为13.21%;未脱脂木瓜中多糖的含量为9.26%;平均回收率为97.04%,RSD为3.52%。[结论]该方法简便易行,稳定性、精密度和重现性良好,可用于资木瓜中多糖的提取和含量测定。

资丘木瓜总黄酮提取工艺研究

[目的]研究资丘木瓜总黄酮提取工艺条件。[方法]以AlCl3试剂测定资丘木瓜总黄酮,在单因素试验的基础上,以总黄酮含量为指标,考察料液比、乙醇浓度、水浴温度等因素对提取效果的影响,并采用正交试验优选出最佳提取条件。[结果]影响资丘木瓜总黄酮的3个因素主次顺序为温度>料液比>乙醇浓度,其中温度和料液比对资丘木瓜黄酮类化合物的提取率影响较明显;最佳提取工艺为浓度50%乙醇为提取剂,水浴温度80℃,料液比1∶8(g/ml),提取时间3 h,提取次数4次;在此条件下,资丘木瓜总黄酮的提取率为1.348%。[结论]该试验确定的最佳工艺稳定性好且简便易行。