In order to reduce deleterious effect on environment,human health and facilities caused by original sulfides, more attention should be paid to biodesulfurization studying for fossil fuels. In this work, eight isolates...In order to reduce deleterious effect on environment,human health and facilities caused by original sulfides, more attention should be paid to biodesulfurization studying for fossil fuels. In this work, eight isolates were characterized by several DNA-based methods such as BOX element polymerase chain reaction( BOX-PCR), enterobacterial repetitive intergenic consensus( ERIC)-PCR and random amplification of polymorphic DNA( RAPD)-PCR. The desulfurization performance was determined by micro-coulometric method,Gibb's assay and barium sulfate test. It was found out that ERIC-PCR displays a much higher inter-strain heterogeneity compared with using BOX. The length of the primer didnot play the most important role in bacterial classification. The combination of the analysis of repetitive-sequence-based polymerase chain reaction ngerprinting and 16 S r DNA was able to provide more effective way in the separation and identification of bacteria.According to the analysis of 16 S r DNA,the more efficient desulfurization strain should belong to Klebsiella variicola.展开更多
The nonsingular H-matrices play an important role in the study of the matrix theory and the iterative method of systems of linear equations, etc. It has always been searched how to verify nonsingular H-matrices. In th...The nonsingular H-matrices play an important role in the study of the matrix theory and the iterative method of systems of linear equations, etc. It has always been searched how to verify nonsingular H-matrices. In this paper, nonsingular H-matrices is studies by applying diagonally dominant matrices, irreducible diagonally dominant matrices and comparison matrices and several practical criteria for identifying nonsingular H-matrices are obtained.展开更多
基金National Natural Science Foundations of China(Nos.41302079,21176145)Project of Shandong Province Higher Educational Science and Technology Program,China(No.J13LD54)
文摘In order to reduce deleterious effect on environment,human health and facilities caused by original sulfides, more attention should be paid to biodesulfurization studying for fossil fuels. In this work, eight isolates were characterized by several DNA-based methods such as BOX element polymerase chain reaction( BOX-PCR), enterobacterial repetitive intergenic consensus( ERIC)-PCR and random amplification of polymorphic DNA( RAPD)-PCR. The desulfurization performance was determined by micro-coulometric method,Gibb's assay and barium sulfate test. It was found out that ERIC-PCR displays a much higher inter-strain heterogeneity compared with using BOX. The length of the primer didnot play the most important role in bacterial classification. The combination of the analysis of repetitive-sequence-based polymerase chain reaction ngerprinting and 16 S r DNA was able to provide more effective way in the separation and identification of bacteria.According to the analysis of 16 S r DNA,the more efficient desulfurization strain should belong to Klebsiella variicola.
文摘The nonsingular H-matrices play an important role in the study of the matrix theory and the iterative method of systems of linear equations, etc. It has always been searched how to verify nonsingular H-matrices. In this paper, nonsingular H-matrices is studies by applying diagonally dominant matrices, irreducible diagonally dominant matrices and comparison matrices and several practical criteria for identifying nonsingular H-matrices are obtained.
