To investigate the modulating effects of survivn antisense oligonucletode (ASODN) on the cell cycle and apoptosis of human hepatocellular carcinoma (HCC) cell line SMMC-7721 and explore its mechanism.Methods Survivin ...To investigate the modulating effects of survivn antisense oligonucletode (ASODN) on the cell cycle and apoptosis of human hepatocellular carcinoma (HCC) cell line SMMC-7721 and explore its mechanism.Methods Survivin ASODN was transfected into SMMC-7721 cells mediated by DOTAP liposomal reagent.Electron microscopy,flow cytometry and RT-PCR were used to detect the changes in cell ultrastructure,apoptosis,cell cycle and the expression of cyclinB1 mRNA,respectively.Results After transfection of survivin ASODN,the expression of cyclinB1 mRNA in the cells significantly increased and increase in G2-M arrest and apoptosis appeared.Meanwhile,the cell ultrastructure had apoptotic changes such as chromatin condensation and apoptotic body formation.Conclusion Survivin ASODN can induce the expression of cyclinB1 that may result in G2-M arrest.Consequently,apoptosis is triggered.Survivin ASODN transfection might be an improtant new treatment for HCC.14 refs,2 figs,1 tab.展开更多
Urban rail transit loops are essential in urban rail transit systems and transportation networks.However,precise requirements and reference standards for rail transit loop design have yet to be established.There are c...Urban rail transit loops are essential in urban rail transit systems and transportation networks.However,precise requirements and reference standards for rail transit loop design have yet to be established.There are certain areas for improvement in planning,construction,and operation.In the planning and design of urban rail transit loops,the scale of the city and the relationship between line operations should be considered to ensure that the line conforms to the city’s operating traffic conditions and can effectively cater to peak passenger flow requirements.This article presents strategies for planning,constructing,and operating urban rail transit loops,laying the foundation for the healthy operation of urban rail transit.展开更多
文摘To investigate the modulating effects of survivn antisense oligonucletode (ASODN) on the cell cycle and apoptosis of human hepatocellular carcinoma (HCC) cell line SMMC-7721 and explore its mechanism.Methods Survivin ASODN was transfected into SMMC-7721 cells mediated by DOTAP liposomal reagent.Electron microscopy,flow cytometry and RT-PCR were used to detect the changes in cell ultrastructure,apoptosis,cell cycle and the expression of cyclinB1 mRNA,respectively.Results After transfection of survivin ASODN,the expression of cyclinB1 mRNA in the cells significantly increased and increase in G2-M arrest and apoptosis appeared.Meanwhile,the cell ultrastructure had apoptotic changes such as chromatin condensation and apoptotic body formation.Conclusion Survivin ASODN can induce the expression of cyclinB1 that may result in G2-M arrest.Consequently,apoptosis is triggered.Survivin ASODN transfection might be an improtant new treatment for HCC.14 refs,2 figs,1 tab.
文摘Urban rail transit loops are essential in urban rail transit systems and transportation networks.However,precise requirements and reference standards for rail transit loop design have yet to be established.There are certain areas for improvement in planning,construction,and operation.In the planning and design of urban rail transit loops,the scale of the city and the relationship between line operations should be considered to ensure that the line conforms to the city’s operating traffic conditions and can effectively cater to peak passenger flow requirements.This article presents strategies for planning,constructing,and operating urban rail transit loops,laying the foundation for the healthy operation of urban rail transit.
