目的检测人心房肌KChIP2和SK2基因表达,并构建相应质粒标准品,为进一步研究基因功能奠定基础。方法提取人心房肌组织总RNA,采用RT-PCR方法获取KChIP2、SK2、GAPDH、β-actin目的片段,与pMD-18Tvector连接成重组质粒,转化到大肠杆菌DH5α...目的检测人心房肌KChIP2和SK2基因表达,并构建相应质粒标准品,为进一步研究基因功能奠定基础。方法提取人心房肌组织总RNA,采用RT-PCR方法获取KChIP2、SK2、GAPDH、β-actin目的片段,与pMD-18Tvector连接成重组质粒,转化到大肠杆菌DH5α中,通过PCR和测序鉴定。提取含目的基因的质粒作为标准品。采用Taqman探针法与SYBR Green I法分别检测人心房肌SK2与KChIP2基因表达和构建标准曲线。结果人心房肌存在KChIP2和SK2基因表达,质粒标准品经过PCR鉴定并测序,与Pubmed数据库完全一致,标准曲线线性关系好,相关系数R2>0.99。结论成功检测人心房肌KChIP2和SK2基因表达,构建的质粒标准品能用于后续实验。展开更多
In this paper,a statistical cluster-based simulation channel model with a finite number of sinusoids is proposed for depicting the multiple-input multiple-output(MIMO)communications in vehicleto-everything(V2X)environ...In this paper,a statistical cluster-based simulation channel model with a finite number of sinusoids is proposed for depicting the multiple-input multiple-output(MIMO)communications in vehicleto-everything(V2X)environments.In the proposed sum-of-sinusoids(SoS)channel model,the waves that emerge from the transmitter undergo line-of-sight(LoS)and non-line-of-sight(NLoS)propagation to the receiver,which makes the model suitable for describing numerous V2X wireless communication scenarios for sixth-generation(6G).We derive expressions for the real and imaginary parts of the complex channel impulse response(CIR),which characterize the physical propagation characteristics of V2X wireless channels.The statistical properties of the real and imaginary parts of the complex CIRs,i.e.,autocorrelation functions(ACFs),Doppler power spectral densities(PSDs),cross-correlation functions(CCFs),and variances of ACFs and CCFs,are derived and discussed.Simulation results are generated and match those predicted by the underlying theory,demonstrating the accuracy of our derivation and analysis.The proposed framework and underlying theory arise as an efficient tool to investigate the statistical properties of 6G MIMO V2X communication systems.展开更多
文摘目的检测人心房肌KChIP2和SK2基因表达,并构建相应质粒标准品,为进一步研究基因功能奠定基础。方法提取人心房肌组织总RNA,采用RT-PCR方法获取KChIP2、SK2、GAPDH、β-actin目的片段,与pMD-18Tvector连接成重组质粒,转化到大肠杆菌DH5α中,通过PCR和测序鉴定。提取含目的基因的质粒作为标准品。采用Taqman探针法与SYBR Green I法分别检测人心房肌SK2与KChIP2基因表达和构建标准曲线。结果人心房肌存在KChIP2和SK2基因表达,质粒标准品经过PCR鉴定并测序,与Pubmed数据库完全一致,标准曲线线性关系好,相关系数R2>0.99。结论成功检测人心房肌KChIP2和SK2基因表达,构建的质粒标准品能用于后续实验。
基金supported by National Natural Science Foundation of China(NSFC)(No.62101274 and 62101275)Natural Science Foundation of Jiangsu Province(BK20210640)Open Research Fund of National Mobile Communications Research Laboratory Southeast University under Grant 2021D03。
文摘In this paper,a statistical cluster-based simulation channel model with a finite number of sinusoids is proposed for depicting the multiple-input multiple-output(MIMO)communications in vehicleto-everything(V2X)environments.In the proposed sum-of-sinusoids(SoS)channel model,the waves that emerge from the transmitter undergo line-of-sight(LoS)and non-line-of-sight(NLoS)propagation to the receiver,which makes the model suitable for describing numerous V2X wireless communication scenarios for sixth-generation(6G).We derive expressions for the real and imaginary parts of the complex channel impulse response(CIR),which characterize the physical propagation characteristics of V2X wireless channels.The statistical properties of the real and imaginary parts of the complex CIRs,i.e.,autocorrelation functions(ACFs),Doppler power spectral densities(PSDs),cross-correlation functions(CCFs),and variances of ACFs and CCFs,are derived and discussed.Simulation results are generated and match those predicted by the underlying theory,demonstrating the accuracy of our derivation and analysis.The proposed framework and underlying theory arise as an efficient tool to investigate the statistical properties of 6G MIMO V2X communication systems.
