In this investigation,sensitive and reproducible methods are described for quantitative determination of deflazacort in the presence of its degradation product.The method was based on high performance liquid chromatog...In this investigation,sensitive and reproducible methods are described for quantitative determination of deflazacort in the presence of its degradation product.The method was based on high performance liquid chromatography of the drug from its degradation product on reverse phase using Acquity UPLC BEH C18columns（1.7 urn,2.1 mm ×150 mm） using acetonitrile and water（40：60 V/V） at a flow rate of 0.2 mL/minute in UPLC.UV detection was performed at 240.1 nm.Deflazacort was subjected to oxidative,acid,base,hydrolytic,thermal and photolytic degradation.The drug was found to be stable in water and thermal stress,as well as under neutral stress conditions.However,forced-degradation study performed on deflazacort showed that the drug degraded under alkaline,acid and photolytic stress.The degradation products were well resolved from the main peak,which proved the stability-indicating power of the method.The developed method was validated as per ICH guidelines with respect to accuracy,linearity,limit of detection,limit of quantification,accuracy,precision and robustness,selectivity and specificity.Apart from the aforementioned,the results of the present study also emphasize the importance of isolation characterization and identification of degradant.Hence,an attempt was made to identify the degradants in deflazacort.One of the degradation products of deflazacort was isolated and identified by the FTIR,NMR and LC-MS study.展开更多
The electronic modification effect of various metal oxides over Pt-Al;O;catalyst andthe relationships between the polarizing force of cations（PFC）and the electrophiliccharacter（EC）and catalytic performances（CP）o...The electronic modification effect of various metal oxides over Pt-Al;O;catalyst andthe relationships between the polarizing force of cations（PFC）and the electrophiliccharacter（EC）and catalytic performances（CP）of promoted Pt catalyst have been studiecby competitive hydrogenation reaction method（CHRM）and test reaction,i.e.hydrogena-tion of benzene and hydrogenolysis of cyclopentane.展开更多
文摘In this investigation,sensitive and reproducible methods are described for quantitative determination of deflazacort in the presence of its degradation product.The method was based on high performance liquid chromatography of the drug from its degradation product on reverse phase using Acquity UPLC BEH C18columns（1.7 urn,2.1 mm ×150 mm） using acetonitrile and water（40：60 V/V） at a flow rate of 0.2 mL/minute in UPLC.UV detection was performed at 240.1 nm.Deflazacort was subjected to oxidative,acid,base,hydrolytic,thermal and photolytic degradation.The drug was found to be stable in water and thermal stress,as well as under neutral stress conditions.However,forced-degradation study performed on deflazacort showed that the drug degraded under alkaline,acid and photolytic stress.The degradation products were well resolved from the main peak,which proved the stability-indicating power of the method.The developed method was validated as per ICH guidelines with respect to accuracy,linearity,limit of detection,limit of quantification,accuracy,precision and robustness,selectivity and specificity.Apart from the aforementioned,the results of the present study also emphasize the importance of isolation characterization and identification of degradant.Hence,an attempt was made to identify the degradants in deflazacort.One of the degradation products of deflazacort was isolated and identified by the FTIR,NMR and LC-MS study.
文摘The electronic modification effect of various metal oxides over Pt-Al;O;catalyst andthe relationships between the polarizing force of cations（PFC）and the electrophiliccharacter（EC）and catalytic performances（CP）of promoted Pt catalyst have been studiecby competitive hydrogenation reaction method（CHRM）and test reaction,i.e.hydrogena-tion of benzene and hydrogenolysis of cyclopentane.
