The desert plant Rhazya stricta has anticancer and antimicrobial properties, and is widely used in indigenous medicines of Saudi Arabia. However, the therapeutic benefits rely on an accurate identification of this spe...The desert plant Rhazya stricta has anticancer and antimicrobial properties, and is widely used in indigenous medicines of Saudi Arabia. However, the therapeutic benefits rely on an accurate identification of this species. The authenticity of R. stricta and other medicinal plants and herbs procured from local markets can be questionable due to a lack of clear phenotypic traits. DNA barcoding is an emerging technology for rapid and accurate species identification. In this study, six candidate chloroplastid barcodes were investigated for the authentication of R. stricta. We compared the DNA sequences from fifty locally collected and five market samples of R. stricta with database sequences of R. stricta and seven closely related species. We found that the coding regions matK, rbcL, rpoB, and rpoC1 were highly similar among the taxa. By contrast, the intergenic spacers psbK-psbI and atpF-atpH were variable loci distinct for the medicinal plant R. stricta. psbK-psbI clearly discriminated R. stricta samples as an efficient single locus marker, whereas a two-locus marker combination comprising psbK-psbI + atpF-atpH was also promising according to results from the Basic Local Alignment Search Tool and a maximum likelihood gene tree generated using PHyML. Two-dimensional DNA barcodes (i.e., QR codes) for the psbK-psbI and psbK-psbI + atpF-atpH regions were created for the validation of fresh or dried R. stricta samples.展开更多
This paper briefly introduces the characteristics and structure of symbol QR two-dimensional code, a detailed analysis of the image processing method to identify QR code of the whole process, and the bilinear mapping ...This paper briefly introduces the characteristics and structure of symbol QR two-dimensional code, a detailed analysis of the image processing method to identify QR code of the whole process, and the bilinear mapping method is applied to image correction, the final steps of decoding are given. The actual test results show that, the design algorithm has theoretical and practical, this recognition system can correctly read QR code, and has high recognition rate and recognition speed, has practical value and application prospect.展开更多
延胡索是我国传统中药材中的常用大宗药材之一,产地多且适应性强,其中浙江省为其主要的道地产区之一.延胡索生产中多采用自繁自种的无性繁殖,易产生种质退化等问题,导致其产量和品质良莠不齐.采用基因条形码ITS2序列检测技术、田间延胡...延胡索是我国传统中药材中的常用大宗药材之一,产地多且适应性强,其中浙江省为其主要的道地产区之一.延胡索生产中多采用自繁自种的无性繁殖,易产生种质退化等问题,导致其产量和品质良莠不齐.采用基因条形码ITS2序列检测技术、田间延胡索性状记录及高效液相色谱分析等技术,对浙江省3个主要品种来源的延胡索浙胡1号(YHS-1)、浙胡2号(YHS-2)和延胡索3号(农户自保种,YHS-3)进行延胡索的基源鉴定、田间性状分析和主要苄基异喹啉型生物碱的含量分析,以筛选浙江地区延胡索的优良品种.基源鉴定数据结果显示,YHS-2与延胡索Corydalis Yanhusuo W. T. Wang(NCBI编号MH260617.1和MH260618.1)的亲缘关系较近,YHS-3次之,而YHS-1亲缘关系较远.田间数据结果显示,与另外2个品种相比,YHS-2具有花多且密,花期短以及块茎产量高的优良生产性状.建立6种苄基异喹啉型生物碱的快速检测方法分析3个品种中生物碱的含量.结果显示,YHS-2中各生物碱的含量显著优于YHS-1和YHS-3(p<0.05).表明3个品种中YHS-2更为适合浙江地区的生存环境,可作为目前延胡索浙江栽培的优质品种推广种植和使用.展开更多
文摘The desert plant Rhazya stricta has anticancer and antimicrobial properties, and is widely used in indigenous medicines of Saudi Arabia. However, the therapeutic benefits rely on an accurate identification of this species. The authenticity of R. stricta and other medicinal plants and herbs procured from local markets can be questionable due to a lack of clear phenotypic traits. DNA barcoding is an emerging technology for rapid and accurate species identification. In this study, six candidate chloroplastid barcodes were investigated for the authentication of R. stricta. We compared the DNA sequences from fifty locally collected and five market samples of R. stricta with database sequences of R. stricta and seven closely related species. We found that the coding regions matK, rbcL, rpoB, and rpoC1 were highly similar among the taxa. By contrast, the intergenic spacers psbK-psbI and atpF-atpH were variable loci distinct for the medicinal plant R. stricta. psbK-psbI clearly discriminated R. stricta samples as an efficient single locus marker, whereas a two-locus marker combination comprising psbK-psbI + atpF-atpH was also promising according to results from the Basic Local Alignment Search Tool and a maximum likelihood gene tree generated using PHyML. Two-dimensional DNA barcodes (i.e., QR codes) for the psbK-psbI and psbK-psbI + atpF-atpH regions were created for the validation of fresh or dried R. stricta samples.
文摘This paper briefly introduces the characteristics and structure of symbol QR two-dimensional code, a detailed analysis of the image processing method to identify QR code of the whole process, and the bilinear mapping method is applied to image correction, the final steps of decoding are given. The actual test results show that, the design algorithm has theoretical and practical, this recognition system can correctly read QR code, and has high recognition rate and recognition speed, has practical value and application prospect.
文摘延胡索是我国传统中药材中的常用大宗药材之一,产地多且适应性强,其中浙江省为其主要的道地产区之一.延胡索生产中多采用自繁自种的无性繁殖,易产生种质退化等问题,导致其产量和品质良莠不齐.采用基因条形码ITS2序列检测技术、田间延胡索性状记录及高效液相色谱分析等技术,对浙江省3个主要品种来源的延胡索浙胡1号(YHS-1)、浙胡2号(YHS-2)和延胡索3号(农户自保种,YHS-3)进行延胡索的基源鉴定、田间性状分析和主要苄基异喹啉型生物碱的含量分析,以筛选浙江地区延胡索的优良品种.基源鉴定数据结果显示,YHS-2与延胡索Corydalis Yanhusuo W. T. Wang(NCBI编号MH260617.1和MH260618.1)的亲缘关系较近,YHS-3次之,而YHS-1亲缘关系较远.田间数据结果显示,与另外2个品种相比,YHS-2具有花多且密,花期短以及块茎产量高的优良生产性状.建立6种苄基异喹啉型生物碱的快速检测方法分析3个品种中生物碱的含量.结果显示,YHS-2中各生物碱的含量显著优于YHS-1和YHS-3(p<0.05).表明3个品种中YHS-2更为适合浙江地区的生存环境,可作为目前延胡索浙江栽培的优质品种推广种植和使用.
基金国家教育部新世纪人才支持计划( the New Century Excellent Talent Foundation from MOE of China under Grant No.NCET- 05- 0897)新疆维吾尔自治区高校自然科学研究计划项目( the Natural Science Research Project of Higher Education of Xinjiang+1 种基金 China under Grant No.XJEDU2004E02 No.XJEDU2006I10) 。
