Long non-coding RNAs(lncRNAs) play important roles in response to various biotic and abiotic stresses. So far, systematic identification and characterization of lncRNAs have been reported in a few model plant species ...Long non-coding RNAs(lncRNAs) play important roles in response to various biotic and abiotic stresses. So far, systematic identification and characterization of lncRNAs have been reported in a few model plant species and major crops, but their roles in abiotic stress response have not yet been reported in common wild rice(Oryza rufipogon). Dongxiang wild rice(DXWR) possesses a high degree of drought resistance and has been well recognized as a precious genetic resource for drought resistant rice breeding. We presented the reference catalog of 1 655 novel lncRNA transcripts in DXWR using strand-specific RNA sequencing and bioinformatics approaches. Meanwhile, a total of 1092 lncRNAs were determined as differentially expressed lncRNAs under drought stress. Quantitative real-time PCR results exhibited a high concordance with RNA sequencing data, which confirmed that the expression patterns of lncRNAs based on RNA sequencing were highly reliable. Furthermore, 8 711 transcripts were predicted as target genes of the differentially expressed lncRNAs. Functional annotation analysis based on Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases showed that the target genes were significantly enriched in cellular and metabolic processes, cell part, binding and plant hormone signal transduction, as well as many other terms related to abiotic stress resistance. These results expanded our understanding of lncRNA biology and provided candidate regulators for genetic improvement of drought resistance in rice cultivars.展开更多
6 Atomic fragment types of organic compound have been defined, and the multilevel atom-pair frequency matrix has been constructed according to the occurrence number in pairs of atomic fragments with different bond len...6 Atomic fragment types of organic compound have been defined, and the multilevel atom-pair frequency matrix has been constructed according to the occurrence number in pairs of atomic fragments with different bond lengths in the molecule. On the basis of them, a novel molecular coding technique： characteristic atom-pair holographic code （CAHC）, is obtained. To some extent, this method exhibits a large number of benefits at the same time. For example, it can calculate 2D molecular topological descriptor easily, operate without difficulty and possess definite physicochemical meaning of 3D molecular structural characterization methods, and may fetch the complicated information of molecule, etc. Therefore, it is appropriate for the study on quantitative structure-property/activity relationship （QSPR/QSAR） of medicines and biological molecules. We attempt in this paper to utilize the method of CAHC to the quantitative prediction of reversed-phase liquid chromatogram （RPLC） retention data of 33 purine derivatives and 24 steroids. The fitting multiple correlation coefficient R2, cross-validated multiple correlation coefficient Q2 and predicted ability Q^2 pred over test set＇s samples of obtained partial least-square （PLS） regression model are respectively 0.990, 0.893 and 0.977, 0.897, 0.941.展开更多
The Great Gatsby narrates a symbolist tragedy in a transitional era.It’s ambivalent characterizationand themes find justifications in the writer’s own ideology of two opposed ideas ,and in his efforts to define andj...The Great Gatsby narrates a symbolist tragedy in a transitional era.It’s ambivalent characterizationand themes find justifications in the writer’s own ideology of two opposed ideas ,and in his efforts to define andjudge subjects of social problems.The ambivalent characterization concerns the writer’s dual response to theprotagonist’s corruption and innocence respectively.Ultimately the writer is convinced that both the corruptionand innocence are justifiable in the morality of his protagonist.Whereas the ambivalent themes dealing withthe American dream attach importance to the social class perspectives and the vision of history,undoubtedlythe writer slides into a compromise with his themes:repudation and approval.Because of the equally strong in-terplay of two opposed ideas,the writer fails to locate a definite position and scale,which results in his imma-ture control in the art of the work.The writer’s characteristic code and achievement are significant becausethey genuinely reveal human being’s fundamental dilemma in social life.展开更多
目的分析引起吉林省2009年手足口病(Hand,Footand Mouth Disease,HFMD)流行的柯萨奇病毒A组16型(Coxsackievirus Group A Type16,CVA16)VP1编码区的基因特征。方法 对2009年分离于吉林省部分HFMD来源的15株CVA16分离株的VP1编码区基因...目的分析引起吉林省2009年手足口病(Hand,Footand Mouth Disease,HFMD)流行的柯萨奇病毒A组16型(Coxsackievirus Group A Type16,CVA16)VP1编码区的基因特征。方法 对2009年分离于吉林省部分HFMD来源的15株CVA16分离株的VP1编码区基因,进行逆转录-聚合酶链反应扩增,并对扩增产物进行序列测定,使用Bioedit软件和MEGA4.0软件对VP1编码区基因进行基因遗传进化及同源性分析。结果吉林省2009年15株CVA16分离株有86.7%(13/15)属于B1a基因亚型,13.3%(2/15)属于B1b基因亚型,并且15株分离株分属于3个相对独立的传播链(Clade1～3),分别由11株、2株、2株组成。3个传播链各自的组内核苷酸和氨基酸同源性均较高,其中较大优势传播链(Clade1)的核苷酸和氨基酸同源性均高达98.2%～100.0%,两个较小传播链均高达100%。结论吉林省2009年监测到至少有2个基因亚型、3个传播链的CVA16引起的HFMD流行,并且呈现以1个B1a基因亚型的优势传播链为主,其他2个小传播链为辅的传播模式。无论是优势传播链还是小传播链,均各自呈现密切的亲缘关系。展开更多
基金国家自然科学基金(批准号:21972136,21676262,21991091)中国科学院百人计划(批准号:Y706071202)+3 种基金中国科学院大连洁净能源创新研究院合作基金(批准号:DNL201908)中国科学院前沿科学重点研究计划(批准号:QYZDB-SSW-JSC040)VR(Swedish Research Council)Starting Grant(批准号:2017-05333)中法国际联合实验室-分子筛资助
基金supported by the National Natural Science Foundation of China (Grant No. 31660386)the Natural Science Foundation of Jiangxi Province for Distinguished Young Scholars (Grant No. 20171BCB23040)+1 种基金the Foundation of Jiangxi Educational Committee (Grant No. GJJ170193)the Sponsored Program for Distinguished Young Scholars in Jiangxi Normal University, China
文摘Long non-coding RNAs(lncRNAs) play important roles in response to various biotic and abiotic stresses. So far, systematic identification and characterization of lncRNAs have been reported in a few model plant species and major crops, but their roles in abiotic stress response have not yet been reported in common wild rice(Oryza rufipogon). Dongxiang wild rice(DXWR) possesses a high degree of drought resistance and has been well recognized as a precious genetic resource for drought resistant rice breeding. We presented the reference catalog of 1 655 novel lncRNA transcripts in DXWR using strand-specific RNA sequencing and bioinformatics approaches. Meanwhile, a total of 1092 lncRNAs were determined as differentially expressed lncRNAs under drought stress. Quantitative real-time PCR results exhibited a high concordance with RNA sequencing data, which confirmed that the expression patterns of lncRNAs based on RNA sequencing were highly reliable. Furthermore, 8 711 transcripts were predicted as target genes of the differentially expressed lncRNAs. Functional annotation analysis based on Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases showed that the target genes were significantly enriched in cellular and metabolic processes, cell part, binding and plant hormone signal transduction, as well as many other terms related to abiotic stress resistance. These results expanded our understanding of lncRNA biology and provided candidate regulators for genetic improvement of drought resistance in rice cultivars.
基金This work was supported by the State Key Laboratory of Chemo/Biosensing and Chemometrics Foundation (No. 05-12-1), Fok-Yingtung Educational Foundation (No. 98-7-6) and Chongqing University Innovation Foundation of Science and Technology ( No. 06-1-1)
文摘6 Atomic fragment types of organic compound have been defined, and the multilevel atom-pair frequency matrix has been constructed according to the occurrence number in pairs of atomic fragments with different bond lengths in the molecule. On the basis of them, a novel molecular coding technique： characteristic atom-pair holographic code （CAHC）, is obtained. To some extent, this method exhibits a large number of benefits at the same time. For example, it can calculate 2D molecular topological descriptor easily, operate without difficulty and possess definite physicochemical meaning of 3D molecular structural characterization methods, and may fetch the complicated information of molecule, etc. Therefore, it is appropriate for the study on quantitative structure-property/activity relationship （QSPR/QSAR） of medicines and biological molecules. We attempt in this paper to utilize the method of CAHC to the quantitative prediction of reversed-phase liquid chromatogram （RPLC） retention data of 33 purine derivatives and 24 steroids. The fitting multiple correlation coefficient R2, cross-validated multiple correlation coefficient Q2 and predicted ability Q^2 pred over test set＇s samples of obtained partial least-square （PLS） regression model are respectively 0.990, 0.893 and 0.977, 0.897, 0.941.
文摘The Great Gatsby narrates a symbolist tragedy in a transitional era.It’s ambivalent characterizationand themes find justifications in the writer’s own ideology of two opposed ideas ,and in his efforts to define andjudge subjects of social problems.The ambivalent characterization concerns the writer’s dual response to theprotagonist’s corruption and innocence respectively.Ultimately the writer is convinced that both the corruptionand innocence are justifiable in the morality of his protagonist.Whereas the ambivalent themes dealing withthe American dream attach importance to the social class perspectives and the vision of history,undoubtedlythe writer slides into a compromise with his themes:repudation and approval.Because of the equally strong in-terplay of two opposed ideas,the writer fails to locate a definite position and scale,which results in his imma-ture control in the art of the work.The writer’s characteristic code and achievement are significant becausethey genuinely reveal human being’s fundamental dilemma in social life.
基金科技部十一五国家科技支撑计划(2008BAI56B02)艾滋病和病毒性肝炎等重大传染病防治科学技术重大专项--传染病传播的相关环境因素监测和预警框架系统(2008ZX10402)+1 种基金日本厚生省Research on Emerging and Re-emerging Infectious Disease研究项目山东省卫生厅重点课题
文摘目的分析引起吉林省2009年手足口病(Hand,Footand Mouth Disease,HFMD)流行的柯萨奇病毒A组16型(Coxsackievirus Group A Type16,CVA16)VP1编码区的基因特征。方法 对2009年分离于吉林省部分HFMD来源的15株CVA16分离株的VP1编码区基因,进行逆转录-聚合酶链反应扩增,并对扩增产物进行序列测定,使用Bioedit软件和MEGA4.0软件对VP1编码区基因进行基因遗传进化及同源性分析。结果吉林省2009年15株CVA16分离株有86.7%(13/15)属于B1a基因亚型,13.3%(2/15)属于B1b基因亚型,并且15株分离株分属于3个相对独立的传播链(Clade1～3),分别由11株、2株、2株组成。3个传播链各自的组内核苷酸和氨基酸同源性均较高,其中较大优势传播链(Clade1)的核苷酸和氨基酸同源性均高达98.2%～100.0%,两个较小传播链均高达100%。结论吉林省2009年监测到至少有2个基因亚型、3个传播链的CVA16引起的HFMD流行,并且呈现以1个B1a基因亚型的优势传播链为主,其他2个小传播链为辅的传播模式。无论是优势传播链还是小传播链,均各自呈现密切的亲缘关系。
