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社区服务创新之路 用“CHEFS+O2O”模式打造智慧社区
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作者 戴卫 《中国房地产》 2015年第10Z期26-29,共4页
房企不能再只靠卖房赚"一锤子买卖"的钱了,得靠服务赚70年"长流水"的钱,这是共识。移动互联网砸碎的只是传统房企物业的锁链,带给他们的却是整个世界。L智慧社区投资发展有限公司(以下简称"L公司")借助... 房企不能再只靠卖房赚"一锤子买卖"的钱了,得靠服务赚70年"长流水"的钱,这是共识。移动互联网砸碎的只是传统房企物业的锁链,带给他们的却是整个世界。L智慧社区投资发展有限公司(以下简称"L公司")借助互联网思维,在逆市中独辟蹊径,致力于打造"CHEFS+O2O"模式的智慧型社区,并创造了逆势而动的神话。 展开更多
关键词 chefs+O2O 逆势而动 市中 新楼盘 收费率 管理面积 用户体验 指线 操作指引 线下
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Eruptive xanthomas in a patient with severe hypertriglyceridemia:A case report
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作者 Ci Ren Ling Zhu +3 位作者 Yan-Chao Niu Lian-Yu Tu Zhou-Feng Jin Jian Zhang 《World Journal of Clinical Cases》 SCIE 2024年第16期2842-2846,共5页
BACKGROUND Xanthomatosis,a metabolic disorder causing yellow growths(xanthomas),poses challenges in lipid metabolism.This case study introduces the first documented instance within China's Yi population,emphasizin... BACKGROUND Xanthomatosis,a metabolic disorder causing yellow growths(xanthomas),poses challenges in lipid metabolism.This case study introduces the first documented instance within China's Yi population,emphasizing the need to explore dietary habits and treatment strategies tailored to this specific community.CASE SUMMARY Xanthomatosis is a metabolic disorder where lipid metabolism goes awry,resulting in the development of yellowish growths called xanthomas.A male patient,47 years of age,from China's Yi population,who is obese,visited our dermatology clinic complaining of widespread,non-painful rashes that have been present for two weeks.The patient works as a chef and has a diet that frequently includes oily and greasy foods.This case represents the initial documentation of xanthomatosis within the Yi population in China,offering a theoretical foundation for understanding dietary patterns and treatment options specific to the Yi community.CONCLUSION The first report of xanthomatosis in the Yi population in China lays a theoretical foundation for understanding Yi dietary patterns and treatment. 展开更多
关键词 Eruptive xanthomas HYPERTRIGLYCERIDEMIA Yi ethnicity DERMATOLOGY Chef Case report
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三种被孢霉的电泳核型分析 被引量:7
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作者 李明春 岳慧琴 邢来君 《菌物系统》 CAS CSCD 北大核心 2000年第1期72-77,共6页
利用等强度均一电场(Contour-clamped Homogeneous Electric Field,CHEF)凝胶电泳技术比较了三种被孢霉菌株及两株诱变菌株的电泳核型。结果显示深黄被孢霉AS3.3410(Mor... 利用等强度均一电场(Contour-clamped Homogeneous Electric Field,CHEF)凝胶电泳技术比较了三种被孢霉菌株及两株诱变菌株的电泳核型。结果显示深黄被孢霉AS3.3410(Mortierella isabellina AS3.3410)及其诱变株M(6-22)、MH(23)具有相同的染色体DNA分子的数目和大小,而与拉曼被抱霉AS3.3413(M.ramanniana AS3.3413)和葡酒色被孢霉AS3.3414(Mvinacea AS3.3414)显示出明显的差异。三种被抱霉明显的染色体带数分别为15条,10条和11条,分离的染色体DNA大小范围大约为390kb-2660Kb,基因组大小分别约为21640Kb、15040Kb、19670Kb。 展开更多
关键词 被孢霉 染色体DNA 电泳核型 CHEF
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黑木耳电泳核型分析 被引量:6
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作者 边银丙 罗信昌 +2 位作者 王斌 金德敏 周启 《菌物系统》 CSCD 北大核心 2000年第1期78-80,共3页
采用等高锁状均质电场(CHEF)凝胶电泳技术,对黑木耳(Auricularia auricula)电泳核型进行分析,以酿酒酵母(Saccharomyces cerivisiae)菌株YPH755和祭酒裂殖酵母(Sch... 采用等高锁状均质电场(CHEF)凝胶电泳技术,对黑木耳(Auricularia auricula)电泳核型进行分析,以酿酒酵母(Saccharomyces cerivisiae)菌株YPH755和祭酒裂殖酵母(Schizosaccharomyces pombe)菌株AS2.214的染色体DNA大小作为分子量标记,估计黑木耳基因组中至少包含9条DNA分子量在850kb至5800kb之间的染色体,多数染色体DNA分子量在1000kb至3400kb之间,基因组大小在22Mb以上。 展开更多
关键词 黑木耳 CHEF电泳 电泳核型 染色体DNA 遗传学
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用CHEF凝胶电泳探测远缘担子菌间原生质体融合后的染色体变异 被引量:5
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作者 曾荣 刘祖同 +1 位作者 张树庭 尤美莲 《微生物学报》 CAS CSCD 北大核心 1996年第3期213-219,共7页
用电场诱导灰盖鬼伞(Coprinus cinereus Co5104,his^-)和佛罗里达侧耳(Pleurotusflorida Pf67, ade^-)两菌株的原生质体融合,获得了两类属间融合子:一类为不断发生分离的单核体,另一类为稳定的双核异核体。利用等高钳位均匀电场(CHEF)... 用电场诱导灰盖鬼伞(Coprinus cinereus Co5104,his^-)和佛罗里达侧耳(Pleurotusflorida Pf67, ade^-)两菌株的原生质体融合,获得了两类属间融合子:一类为不断发生分离的单核体,另一类为稳定的双核异核体。利用等高钳位均匀电场(CHEF)凝胶电泳技术比较融合子与两亲本的染色体长度多型性(CLP),显示出融合子的染色体变异以整条染色体的丢失为主。结合形态观察和DAPI核荧光染色的结果,推测单核融合子在胞质融合后很可能发生了核融合,但来自亲本Pf67的染色体不断丢失,最后融合子的遗传组成以来自Co5104的染色体为主;而双核融合子在质配之后可能并未发生核配,不过,来自各亲本的染色体最终均只有部分得以保留在融合子中。 展开更多
关键词 担子菌 原生质体融合 CHEF凝胶电泳 染色体
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酿酒酵母染色体DNA样品制备和CHEF分析 被引量:1
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作者 边银丙 翁曼丽 +3 位作者 罗信昌 金德敏 周启 王斌 《菌物系统》 CSCD 北大核心 1999年第3期284-287,共4页
等高锁状均匀电场(contolllsclampedhomogeneouselectncfield,CHED电泳技术与分子杂交、酵母人工染色体(yeastartificialchromosome,YAC)等分子生物学技术的有机结合,推动了真菌电泳核型、染色体作图和染色体DNA长度多型性(chrom... 等高锁状均匀电场(contolllsclampedhomogeneouselectncfield,CHED电泳技术与分子杂交、酵母人工染色体(yeastartificialchromosome,YAC)等分子生物学技术的有机结合,推动了真菌电泳核型、染色体作图和染色体DNA长度多型性(chromosomalDNAlellgthpolymorphism,CLP... 展开更多
关键词 酿酒酵母 原生质体 染色体 DNA样品 CHEF分析
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Analysis of the Abnormal Segregation of Pathogenicity in Magnaporthe grisea by Using a Genetic Cross of Oryza and Eleusine Isolates 被引量:3
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作者 YIN Liang-fen LUO Chao-xi +1 位作者 Kusaba Motoaki Yaegashi Hiroshi 《Agricultural Sciences in China》 CAS CSCD 2010年第3期383-391,共9页
A genetic cross between Oryza isolate Y93-164a-1 and Eleusine isolate SA98-4 was established, and the pathogenicity of 151 F1 progeny isolates was investigated on both host plants rice and finger millet. Results showe... A genetic cross between Oryza isolate Y93-164a-1 and Eleusine isolate SA98-4 was established, and the pathogenicity of 151 F1 progeny isolates was investigated on both host plants rice and finger millet. Results showed that the segregation of pathogenicity in this genetic cross was abnormal, i.e., most of the progeny isolates were nonpathogenic on both host plants. However, no abnormal segregation was observed when middle repetitive sequence MGR586 and 31 single-copy RFLP markers from all of the chromosomes were genetically analyzed. At the same time, comparison of the chromosomal organization among two pairs of parental isolates did not find any genomic abnormity. These results suggested that the "abnormal" inheritance of pathogenicity in this cross was most likely due to the reassortment of numerous host species specificity genes but not the biased segregation of the host species specificity genes. The host species specificities in M. grisea were likely to be multigenically controlled, at least in the genetic cross involving rice pathogen and the grasses pathogen other than rice. 展开更多
关键词 Magnaporthe grisea rice blast fungus host species specificity PATHOGENICITY contour-clamped homogeneous electric field (CHEF) electrophoresis Southern hybridization
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Master chef阿兰
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作者 王景晨 汤君华 《烹调知识》 2011年第5Z期66-66,共1页
阿兰老师是我迄今为止结识的最敬业、最爱岗的外国教师。他在里昂的一所厨艺学校任教,这所厨艺学校招收来自世界各地的学员,有中国、韩国、日本、英国、加拿大、瑞士等各国的学员,我有幸分被在了他的班。
关键词 阿兰 MASTER chef 牛里脊 一所 王先生 材料准备 校门口 早上好 剔骨 泰坦尼克号
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Do Not Scold the Chef While Enjoying the Dishes
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《China Textile》 2010年第9期22-23,共2页
The U.S.Department of Commerce’s (DOC’s) International Trade Administration has issued an anti-dumping order on certain Chinese electric blankets that it says were dumped on the U.S. market at
关键词 Do Not Scold the Chef While Enjoying the Dishes
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金属离子响应型荧光传感分子的设计原理及研究进展 被引量:21
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作者 张鹏 张有明 +2 位作者 林奇 姚虹 魏太保 《有机化学》 SCIE CAS CSCD 北大核心 2014年第7期1300-1321,共22页
荧光传感凭借其高灵敏度、可实现远程监测和实时性等优越性而广受关注,在离子识别中常被用于离子识别信号的输出.随着主客体化学的迅速发展,许多具有良好性能的离子响应型荧光探针相继被报道,从分子内电荷转移(ICT)、光诱导的电子转移(P... 荧光传感凭借其高灵敏度、可实现远程监测和实时性等优越性而广受关注,在离子识别中常被用于离子识别信号的输出.随着主客体化学的迅速发展,许多具有良好性能的离子响应型荧光探针相继被报道,从分子内电荷转移(ICT)、光诱导的电子转移(PET)、荧光共振能量转移(FRET)、激发态分子内质子转移(ESIPT)、激基缔合物的生成/消失、螯合作用导致的荧光增强(CHEF)等不同机理对荧光传感型离子识别受体的设计思路进行了理论阐释,归纳总结了近5年来相关文献报道,阐述了其研究现状和研究进展,并展望了该领域的研究方向. 展开更多
关键词 荧光传感 离子识别 分子内电荷转移(ICT) 光诱导的电子转移(PET) 荧光共振能量转移(FRET) 激发态分 子内质子转移(ESIPT) 激基缔合物 荧光增强(CHEF)
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CHO细胞高效表达载体的优化 被引量:1
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作者 刘珊 刘志刚 +1 位作者 张国强 俞炜源 《军事医学科学院院刊》 CSCD 北大核心 2006年第4期301-305,共5页
目的:研究分析中国仓鼠EF1-α基因的转录调控序列,以构建新型高效表达载体。方法:利用常规分子生物学技术,构建了一系列基于CHEF1-α基因的非翻译区调控序列元件的新型真核表达载体。以组织型纤溶酶原激活剂突变体(k2tPA)作为报告基因,... 目的:研究分析中国仓鼠EF1-α基因的转录调控序列,以构建新型高效表达载体。方法:利用常规分子生物学技术,构建了一系列基于CHEF1-α基因的非翻译区调控序列元件的新型真核表达载体。以组织型纤溶酶原激活剂突变体(k2tPA)作为报告基因,利用本室已建立的CHO-dhfr--Z+定点整合表达系统比较载体表达外源基因的能力,挑选表达量高的载体。进一步添加翻译增强子(H213及V163)序列,再进行评价。结果:CHEF1-α5′UTR及启动子+3′UTR 1.65 kb、CHEF1-α5′UTR及启动子+3′UTR 2.7 kb这2种组合均可有效提高目的基因tPA的表达,表达效率与对照载体相比分别提高了65.9%和54.5%。在添加翻译增强子V163后,载体pMCEdEF53Ⅲ-163 frt-k2 tPA的表达效率为对照载体的2.65倍。结论:该载体系统在重组蛋白药物的研发方面具有良好的应用前景。 展开更多
关键词 CHEF1—α基因 CHO/dhfr^-细胞 基因表达 组织型纤溶酶原激活物
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A Novel and Simple Molecular Probe Sensing Hg(II) in Living Cells by Chelation-enhanced Fluorescence 被引量:1
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作者 Meng,Qinghua Cheng,Sen +1 位作者 Lan,Minbo Wei,Gang 《Chinese Journal of Chemistry》 SCIE CAS CSCD 2012年第2期249-253,共5页
Mercury is currently widely used in industries which leads to various means of Hg(II) waste exposure and its accumulation in organisms will cause neurological damages. Thus, there is a great need for the design of p... Mercury is currently widely used in industries which leads to various means of Hg(II) waste exposure and its accumulation in organisms will cause neurological damages. Thus, there is a great need for the design of probes or sensors with high sensitivity and selectivity for detecting and monitoring Hg(II) at physiological pH. Thus a novel and simple molecular probe P1 was prepared from 1,1'-(1,3-phenylene)-bis(2,4-pentanedionato) for sensing Hg(II) via chelation-enhanced fluorescence (CHEF) mechanism. The probe indicated a selectively fluorescent response to Hg(II) in aqueous media excited by the ultraviolet light of 254 nm. The recognition mechanism was further studied by semi-empirical AM1 and molecular mechanics MM+ methods in HyperChem 8.0. The calculation indicated a tetrahedron coordination geometry for Hg(II) and a chair-like configuration for the total molecule. The fluorescent images sensing Hg(II) in living mouse fibroblast cells by the probe were obtained by fluorescence microscope. 展开更多
关键词 MERCURY PROBE semirigid chelation-enhanced fluorescence (CHEF) fluorescent image
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Syntheses and structures of tetranuclear Zn(Ⅱ) complexes with in situ generated macrocyclic Schiff base ligands:Applications in Zn^(2+) sensing 被引量:1
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作者 Cai-Xia Ding Chang-Hua He Yong-Shu Xie 《Chinese Chemical Letters》 SCIE CAS CSCD 2013年第6期463-466,共4页
Three novel Zn(II) complexes,[Zn4L1Cl4]-3H2O(1),[Zn4L2Cl4]-2DMF(2) and[Zn4L^3Cl4]H2O(3),have been synthesized and structurally characterized.In these complexes,interesting 32-membered dodecadentate macrocyclic... Three novel Zn(II) complexes,[Zn4L1Cl4]-3H2O(1),[Zn4L2Cl4]-2DMF(2) and[Zn4L^3Cl4]H2O(3),have been synthesized and structurally characterized.In these complexes,interesting 32-membered dodecadentate macrocyclic ligands were generated in situ by '2 + 2' type condensation reactions between a tetraamine and various dialdehydes.All the complexes are isostructurally tetranuclear Zn(Ⅱ) complexes,containing endogenous alkoxo and phenoxo bridges.Applications of the macrocyclic ligands as Zn^2+ sensors have been investigated.Take H4L^1 for example,it exhibits a 4-fold fluorescence enhancement upon the addition of 2 equiv.of Zn^2+ in MeOH. 展开更多
关键词 Macrocyclic ligand Schiff base Zn(Ⅱ) complexes Fluorescent enhancement CHEF effect
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云端服务器的自动化环境构筑工具——Chef
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作者 刘红 《信息与电脑(理论版)》 2015年第22期28-30,共3页
随着企业信息化的发展,越来越多的大型企业拥有自己的服务器集群。现在,拥有上百台服务器、超千台虚拟机的企业越来越多。随着服务器集群的规模不断增大,对服务器进行批量安装、批量配置和批量管理的需求也越来越强烈。在该背景下,Chef... 随着企业信息化的发展,越来越多的大型企业拥有自己的服务器集群。现在,拥有上百台服务器、超千台虚拟机的企业越来越多。随着服务器集群的规模不断增大,对服务器进行批量安装、批量配置和批量管理的需求也越来越强烈。在该背景下,Chef作为新一代的服务器自动化环境安装部署工具,能为企业进行快速自动化部署起到非常大的作用。通过介绍Chef是什么、Chef的基本概念及Chef的安装和运行,并通过一些实例让读者对Chef有更深的认识,并能结合例子掌握Chef的操作流程。 展开更多
关键词 RUBY Cookbook RECIPE KNIFE NODE Chef
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THE ACCIDENTAL CHEF
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作者 刘珏 《The World of Chinese》 2015年第5期68-69,共2页
A turn of the century doctor changes culinary culture forever民国女厨神的美食秘籍是如何轰动美国的?Born the ninth granddaughter in a respectable,traditional family in 1889 in Nanjing,Buwei Yang(杨步伟)was taught that p... A turn of the century doctor changes culinary culture forever民国女厨神的美食秘籍是如何轰动美国的?Born the ninth granddaughter in a respectable,traditional family in 1889 in Nanjing,Buwei Yang(杨步伟)was taught that proper ladies do not belong in the kitchen.Her purpose in life was to get married and be the mistress of her own household,engaged to her cousin before she was 展开更多
关键词 THE ACCIDENTAL CHEF DOWN
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A Chef for 34 Years
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作者 CUI YUFEN PAN ZHONGMING 《Women of China》 1998年第5期46-47,共2页
FOREIGNERS who have been to Beijing are probably familiar with the Beijing International Hotel near the main railway station. It’s the four-star hotel where I work as a chief cook. My job is to manage nine kitchens—... FOREIGNERS who have been to Beijing are probably familiar with the Beijing International Hotel near the main railway station. It’s the four-star hotel where I work as a chief cook. My job is to manage nine kitchens—both Chinese and Western. I have been a chef for 34 years. After graduated from school in 1964, I studied Chinese cuisine for three years. I believed that it was a job worth doing. In the past, people looked down on this occupation; they thought it was a low-class job because cooks were always serving other people. I, however, found the job very interesting. After all, I reasoned, everyone needs to eat. If we could make eating into a kind of art or a science, eating could become an act of culture. People engaged in the food industry treat the act of eating with a respect that most people cooking at home do not feel. But for those of us who work in prestigious hotels whose guests come from all over the world and have different customs and tastes, it is our job to put the Chinese cooking 展开更多
关键词 A Chef for 34 Years
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