Ligustri lucidi Fructus is a traditional Chinese medicine and possesses various bioactivities,including hypoglycemic effect.Ligustrum lucidum Ait flowers are poorly investigated.Thus,we hypothesized that L.lucidum flo...Ligustri lucidi Fructus is a traditional Chinese medicine and possesses various bioactivities,including hypoglycemic effect.Ligustrum lucidum Ait flowers are poorly investigated.Thus,we hypothesized that L.lucidum flowers also could have hypoglycemic effect.Chemical composition and glucose uptake effect of L.lucidum flowers on 3T3-L1 adipocytes were investigated.In this study,the components of L.lucidum flowers were investigated by various chromatographic and spectroscopic methods and the effects of L.lucidum flowers on the induction of glucose uptake were investigated by 3T3-L1 adipocytes.Seven compounds were isolated and identified from L.lucidum flowers,including ursolic acid(1),kaempferol-7-O-α-Lrhamnoside(2,KR),β-sitosterol(3),β-daucosterol(4),kaempferitrin(5,KF),10-hydroxy oleuropein(6,HO),and kaempferol-3-O-β-D-glucopyranoside-7-α-L-rhamnopyranoside(7,KGR).The results of glucose uptake showed that total extract(TE),KR,KF and HO from L.lucidum flowers significantly enhanced glucose uptake.This study demonstrated that compounds 2,5-7 were isolated for the first time from this plant and compounds 2 and 7 were isolated for the first time in genus Ligustrum,L.lucidum flowers,KR,KF and HO may possess potential hypoglycemic effect.展开更多
This paper reports that Al1-xInxN epilayers were grown on GaN template by metalorganic chemical vapor deposition with an In content of 7%--20%. X-ray diffraction results indicate that all these Al1-xInxN epilayers hav...This paper reports that Al1-xInxN epilayers were grown on GaN template by metalorganic chemical vapor deposition with an In content of 7%--20%. X-ray diffraction results indicate that all these Al1-xInxN epilayers have a relatively low density of threading dislocations. Rutherford backscattering/channeling measurements provide the exact compositional information and show that a gradual variation in composition of the Al1-xInxN epilayer happens along the growth direction. The experimental results of optical reflection clearly show the bandgap energies of Al1-xInxN epilayers. A bowing parameter of 6.5~eV is obtained from the compositional dependence of the energy gap. The cathodoluminescence peak energy of the Al1-xInxN epilayer is much lower than its bandgap, indicating a relatively large Stokes shift in the Al1-xInxN sample.展开更多
目的:探究款冬花经烟草甲虫蛀蚀后化学成分的变化。方法:测定未虫蛀款冬花、烟草甲虫蛀蚀后的款冬花及烟草甲虫排泄物的氢核磁共振(^(1)H nuclear magnetic resonance,^(1)H NMR)图谱并进行化合物指认归属。采用主成分分析(principal co...目的:探究款冬花经烟草甲虫蛀蚀后化学成分的变化。方法:测定未虫蛀款冬花、烟草甲虫蛀蚀后的款冬花及烟草甲虫排泄物的氢核磁共振(^(1)H nuclear magnetic resonance,^(1)H NMR)图谱并进行化合物指认归属。采用主成分分析(principal component analysis,PCA)、偏最小二乘法-判别分析(partial least squares discriminate analysis,PLS-DA)和正交偏最小二乘法-判别分析(orthogonal partial least squares discriminate analysis,OPLS-DA)对虫蛀款冬花进行鉴别。根据变量重要性投影(variable importance in the projection,VIP)明确变化的化学成分,并采用t检验加以验证。结果:根据^(1)H NMR图谱共指认33个化合物。多元统计分析结果表明,未虫蛀款冬花、烟草甲虫蛀蚀后的款冬花和排泄物部分可分别聚为一类。从化学成分含量的差异结果看,款冬花被虫蛀后款冬酮的含量下降,在排泄物中异亮氨酸、缬氨酸等氨基酸的含量升高,绿原酸、异绿原酸等款冬花的次生代谢产物含量下降。结论:首次将^(1)H NMR结合多元统计分析用于中药材虫蛀后化学成分的变化研究,为中药材变质现象的研究提供了新的思路和方法。展开更多
目的:基于分子对接分析复方一枝黄花喷雾剂化学成分与HIV-1蛋白酶和组织蛋白酶L的结合情况。方法:利用TCMSP(Traditional Chinese Medicine Systems Pharmacology)结合文献构建复方一枝黄花喷雾剂小分子配体库,采用Surflex-Dock模块完...目的:基于分子对接分析复方一枝黄花喷雾剂化学成分与HIV-1蛋白酶和组织蛋白酶L的结合情况。方法:利用TCMSP(Traditional Chinese Medicine Systems Pharmacology)结合文献构建复方一枝黄花喷雾剂小分子配体库,采用Surflex-Dock模块完成复方一枝黄花喷雾剂与HIV-1蛋白酶和组织蛋白酶L的分子对接分析。通过Ligplot计算核心成分与HIV-1蛋白酶和组织蛋白酶L形成的氢键、疏水作用。结果:获得复方一枝黄花喷雾剂活性成分74个,通过分子对接发现,26个活性成分与HIV-1蛋白酶有较好的结合(Total score>7),5个活性成分与Cathepsin L蛋白酶有较好的结合(Total score>7)。Diosmetin与HIV-1蛋白酶相互作用形成四个氢键(Gly27,Gly48,Gly49,Arg8),Harmonyl与HIV-1蛋白酶相互作用形成两个氢键(Arg8,Asp30)。结论:本研究初步筛选复方一枝黄花喷雾剂具有抗HIV-1蛋白酶和组织蛋白酶L的化学成分,为抗病毒药物的开发提供思路。展开更多
基金This work was supported by National Natural Science Foundation of China(31900292)Key Project in Science and Technology Agency of Henan Province(182102410083).
文摘Ligustri lucidi Fructus is a traditional Chinese medicine and possesses various bioactivities,including hypoglycemic effect.Ligustrum lucidum Ait flowers are poorly investigated.Thus,we hypothesized that L.lucidum flowers also could have hypoglycemic effect.Chemical composition and glucose uptake effect of L.lucidum flowers on 3T3-L1 adipocytes were investigated.In this study,the components of L.lucidum flowers were investigated by various chromatographic and spectroscopic methods and the effects of L.lucidum flowers on the induction of glucose uptake were investigated by 3T3-L1 adipocytes.Seven compounds were isolated and identified from L.lucidum flowers,including ursolic acid(1),kaempferol-7-O-α-Lrhamnoside(2,KR),β-sitosterol(3),β-daucosterol(4),kaempferitrin(5,KF),10-hydroxy oleuropein(6,HO),and kaempferol-3-O-β-D-glucopyranoside-7-α-L-rhamnopyranoside(7,KGR).The results of glucose uptake showed that total extract(TE),KR,KF and HO from L.lucidum flowers significantly enhanced glucose uptake.This study demonstrated that compounds 2,5-7 were isolated for the first time from this plant and compounds 2 and 7 were isolated for the first time in genus Ligustrum,L.lucidum flowers,KR,KF and HO may possess potential hypoglycemic effect.
基金supported by the National Natural Science Foundation of China (Grant Nos. 60776047, 60506001, 60476021, 60576003 and 60836003)
文摘This paper reports that Al1-xInxN epilayers were grown on GaN template by metalorganic chemical vapor deposition with an In content of 7%--20%. X-ray diffraction results indicate that all these Al1-xInxN epilayers have a relatively low density of threading dislocations. Rutherford backscattering/channeling measurements provide the exact compositional information and show that a gradual variation in composition of the Al1-xInxN epilayer happens along the growth direction. The experimental results of optical reflection clearly show the bandgap energies of Al1-xInxN epilayers. A bowing parameter of 6.5~eV is obtained from the compositional dependence of the energy gap. The cathodoluminescence peak energy of the Al1-xInxN epilayer is much lower than its bandgap, indicating a relatively large Stokes shift in the Al1-xInxN sample.
文摘目的:探究款冬花经烟草甲虫蛀蚀后化学成分的变化。方法:测定未虫蛀款冬花、烟草甲虫蛀蚀后的款冬花及烟草甲虫排泄物的氢核磁共振(^(1)H nuclear magnetic resonance,^(1)H NMR)图谱并进行化合物指认归属。采用主成分分析(principal component analysis,PCA)、偏最小二乘法-判别分析(partial least squares discriminate analysis,PLS-DA)和正交偏最小二乘法-判别分析(orthogonal partial least squares discriminate analysis,OPLS-DA)对虫蛀款冬花进行鉴别。根据变量重要性投影(variable importance in the projection,VIP)明确变化的化学成分,并采用t检验加以验证。结果:根据^(1)H NMR图谱共指认33个化合物。多元统计分析结果表明,未虫蛀款冬花、烟草甲虫蛀蚀后的款冬花和排泄物部分可分别聚为一类。从化学成分含量的差异结果看,款冬花被虫蛀后款冬酮的含量下降,在排泄物中异亮氨酸、缬氨酸等氨基酸的含量升高,绿原酸、异绿原酸等款冬花的次生代谢产物含量下降。结论:首次将^(1)H NMR结合多元统计分析用于中药材虫蛀后化学成分的变化研究,为中药材变质现象的研究提供了新的思路和方法。
文摘目的:基于分子对接分析复方一枝黄花喷雾剂化学成分与HIV-1蛋白酶和组织蛋白酶L的结合情况。方法:利用TCMSP(Traditional Chinese Medicine Systems Pharmacology)结合文献构建复方一枝黄花喷雾剂小分子配体库,采用Surflex-Dock模块完成复方一枝黄花喷雾剂与HIV-1蛋白酶和组织蛋白酶L的分子对接分析。通过Ligplot计算核心成分与HIV-1蛋白酶和组织蛋白酶L形成的氢键、疏水作用。结果:获得复方一枝黄花喷雾剂活性成分74个,通过分子对接发现,26个活性成分与HIV-1蛋白酶有较好的结合(Total score>7),5个活性成分与Cathepsin L蛋白酶有较好的结合(Total score>7)。Diosmetin与HIV-1蛋白酶相互作用形成四个氢键(Gly27,Gly48,Gly49,Arg8),Harmonyl与HIV-1蛋白酶相互作用形成两个氢键(Arg8,Asp30)。结论:本研究初步筛选复方一枝黄花喷雾剂具有抗HIV-1蛋白酶和组织蛋白酶L的化学成分,为抗病毒药物的开发提供思路。
