Chlorophytum leaves were treated with three kinds of chemical mutagen, EMS, DES and NaN3 with different concentration to obtain the variation materials with excellent properties. The results showed that the genetic si...Chlorophytum leaves were treated with three kinds of chemical mutagen, EMS, DES and NaN3 with different concentration to obtain the variation materials with excellent properties. The results showed that the genetic similarity coefficient of variation plants with EMS treatment was between 0.648 and 0.868, with NaN3 treatment was between 0.598 and 0.859, and with DES treatment was between 0.668 and 0.904, of which the mutagenic effects with 0.8% EMS, 250 mg. L-1 NaN3 and 0.3% DES on chloro- phytum were the best. ISSR molecular marker technique was used to analyze their genetic diversities. Total 392 polymorphic bands were obtained through 18 ISSR primers. Polymorphic ratio was 72.4%, which showed that DNA mutation took place in various degrees展开更多
Epicuticular wax forms the outermost protective barrier of the aerial surfaces of land plants, working in concert with other components of the plant cuticle to prevent uncontrolled loss of water and to provide protect...Epicuticular wax forms the outermost protective barrier of the aerial surfaces of land plants, working in concert with other components of the plant cuticle to prevent uncontrolled loss of water and to provide protection against an array of external environmental stress. In this study, chemically- mutagenized populations of rice （Oryza sativa L.） derived from approximately 4 750 M2 families were screened for adhesion of water droplets resulting in a wet leaf/glossy （wig） phenotype. Mutants were identified in 11 independently-derived M2 families. Scanning electron microscopy analysis confirmed the association of the wig phenotype with changes in the epicuticular wax crystals of these plants. The phenotypes of five mutants （7-17A, 26.1, 524.2, 680.2, and 843.1） were confirmed to be the result of single recessive gene mutation. Evaluation of mutants from 3 （6-1A, 7-17A, and 11-39A） of 11 M2 families revealed significant reductions （〉 50%） in surface wax content and increases in cuticle membrane permeability.展开更多
基金Supported by Heilongjiang Provincial Youth Science and Technology Fund (QC07C51)Innovation Project of Northeast Agricultural University (CX2004-2)
文摘Chlorophytum leaves were treated with three kinds of chemical mutagen, EMS, DES and NaN3 with different concentration to obtain the variation materials with excellent properties. The results showed that the genetic similarity coefficient of variation plants with EMS treatment was between 0.648 and 0.868, with NaN3 treatment was between 0.598 and 0.859, and with DES treatment was between 0.668 and 0.904, of which the mutagenic effects with 0.8% EMS, 250 mg. L-1 NaN3 and 0.3% DES on chloro- phytum were the best. ISSR molecular marker technique was used to analyze their genetic diversities. Total 392 polymorphic bands were obtained through 18 ISSR primers. Polymorphic ratio was 72.4%, which showed that DNA mutation took place in various degrees
基金supported by United States Department of Agriculture-Agricultural Research Service Current Research Information System Project 5306-21000021-00DMars, Inc. Cooperative Research and Development Agreement (No. 58-3K95-2-1563)
文摘Epicuticular wax forms the outermost protective barrier of the aerial surfaces of land plants, working in concert with other components of the plant cuticle to prevent uncontrolled loss of water and to provide protection against an array of external environmental stress. In this study, chemically- mutagenized populations of rice （Oryza sativa L.） derived from approximately 4 750 M2 families were screened for adhesion of water droplets resulting in a wet leaf/glossy （wig） phenotype. Mutants were identified in 11 independently-derived M2 families. Scanning electron microscopy analysis confirmed the association of the wig phenotype with changes in the epicuticular wax crystals of these plants. The phenotypes of five mutants （7-17A, 26.1, 524.2, 680.2, and 843.1） were confirmed to be the result of single recessive gene mutation. Evaluation of mutants from 3 （6-1A, 7-17A, and 11-39A） of 11 M2 families revealed significant reductions （〉 50%） in surface wax content and increases in cuticle membrane permeability.
