Based on seven newly-collected specimens, we provide an expanded description for the rare Chinese snake Opisthotropis cheni. The new specimens are consistent with the type series in scale counts and body dimensions. H...Based on seven newly-collected specimens, we provide an expanded description for the rare Chinese snake Opisthotropis cheni. The new specimens are consistent with the type series in scale counts and body dimensions. However, two individuals lack yellow cross-bands that are apparent in the type specimens. A key to the ten Chinese species of Opisthotropis is provided.展开更多
The complete 15,831 bp nucleotide sequence of the mitochondrial genome from Elimaea cheni(Phaneropterinae)was determined.The putative initiation codon for cox1 was TTA.The phylogeny of Orthoptera based on different mt...The complete 15,831 bp nucleotide sequence of the mitochondrial genome from Elimaea cheni(Phaneropterinae)was determined.The putative initiation codon for cox1 was TTA.The phylogeny of Orthoptera based on different mtDNA datasets were analyzed with maximum likelihood(ML)and Bayesian inference(BI).When all 37 genes(mtDNA)were analyzed simultaneously,the monophyly of Caelifera and Ensifera were recovered in the context of our taxon sampling.The phylogeny of Orthoptera was largely consistent with previous phylogenetic hypotheses.Rhaphidophoridae to be a sister group of Tettigoniidae,and the relationships among four subfamilies of Tettigoniidae were(Phaneropterinae +(Conocephalinae +(Bradyporinae + Tettigoniinae))).Pyrgomorphidae was the most basal group of Caelifera.The relationships among six acridid subfamilies were(Oedipodinae +(Acridinae +(Gomphocerinae +(Oxyinae +(Calliptaminae + Cyrtacanthacridinae))))).However,we did not recover a monophyletic Grylloidea.Myrmecophilidae clustered into one clade with Gryllotalpidae instead of with Gryllidae.ML and BI analyses of all protein coding genes(using all nucleotide sequence data or ex-cluding the third codon position,and amino acid sequences)revealed a topology identical to that of the entire mtDNA genome dataset.However,22 tRNAs genes excluding the DHU loop and TψC loop(TRNA),and two rRNA genes(RRNA)perform poorly when analyzed as single dataset.Our results suggest that the best phylogenetic inferences were ML and BI methods based on total mtDNA.Excluding tRNA genes,rRNA genes and the third codon position of protein coding genes from dataset and converting nucleotide sequences to amino acid sequences do not positively affect phylogenetic reconstruction.展开更多
基金partly funded by the National Natural Science Foundation of China(NSFC30670236,NFSC30970334)the Program for New Century Excellent Talents in University,the Ministry of Education of China(NCET-08-0908)Sichuan Youth Sciences and Technology Foundation of China(08ZQ026-006)
文摘Based on seven newly-collected specimens, we provide an expanded description for the rare Chinese snake Opisthotropis cheni. The new specimens are consistent with the type series in scale counts and body dimensions. However, two individuals lack yellow cross-bands that are apparent in the type specimens. A key to the ten Chinese species of Opisthotropis is provided.
基金supported by the National Natural Science Foundation of China(No.30670279 and 30670252)Talent Foundation of Hebei University(No.2008-138)
文摘The complete 15,831 bp nucleotide sequence of the mitochondrial genome from Elimaea cheni(Phaneropterinae)was determined.The putative initiation codon for cox1 was TTA.The phylogeny of Orthoptera based on different mtDNA datasets were analyzed with maximum likelihood(ML)and Bayesian inference(BI).When all 37 genes(mtDNA)were analyzed simultaneously,the monophyly of Caelifera and Ensifera were recovered in the context of our taxon sampling.The phylogeny of Orthoptera was largely consistent with previous phylogenetic hypotheses.Rhaphidophoridae to be a sister group of Tettigoniidae,and the relationships among four subfamilies of Tettigoniidae were(Phaneropterinae +(Conocephalinae +(Bradyporinae + Tettigoniinae))).Pyrgomorphidae was the most basal group of Caelifera.The relationships among six acridid subfamilies were(Oedipodinae +(Acridinae +(Gomphocerinae +(Oxyinae +(Calliptaminae + Cyrtacanthacridinae))))).However,we did not recover a monophyletic Grylloidea.Myrmecophilidae clustered into one clade with Gryllotalpidae instead of with Gryllidae.ML and BI analyses of all protein coding genes(using all nucleotide sequence data or ex-cluding the third codon position,and amino acid sequences)revealed a topology identical to that of the entire mtDNA genome dataset.However,22 tRNAs genes excluding the DHU loop and TψC loop(TRNA),and two rRNA genes(RRNA)perform poorly when analyzed as single dataset.Our results suggest that the best phylogenetic inferences were ML and BI methods based on total mtDNA.Excluding tRNA genes,rRNA genes and the third codon position of protein coding genes from dataset and converting nucleotide sequences to amino acid sequences do not positively affect phylogenetic reconstruction.
