The Effect of Air Pollution on Chenopodium album L. Pollen Structure

Air pollution is harm and discomfort to human or other living organisms, it also causes damage to the environment. The aim of this project was to study the effect of air pollutions on structure and pollen grains development in Chenopodium album. Anthers of Chenopodium album L. were collected at different stages of development from control （less polluted） and polluted areas （mainly SO2, NO2, CO and APM）. Structure and development of pollen grains were studied and compared. The effects of pollution on pollen structure was investigated under Light and Scanning electron microscopy and the results showed that when pollen grains were exposed to polluted air they became abnormality in form and covered with large amounts of pollutants compared to control ones. Pollen abnormalities were seen as irregularity, shrinkage, thinning and breakage of the exine. Cellular material release was induced also. The data presented suggest that prolonged exposures of plants to air pollution may cause different biological effects at the cellular tissue and organ levels.

藜草花粉变应原免疫活性分析

目的：分析藜草花粉变应原成分及其变应原性、免疫原性．方法：用Sephacryl S-200HR葡聚糖凝胶层析法分离、纯化藜草花粉变应原，通过十二烷硫酸钠-聚丙烯酰胺凝胶电脉法（SDS—PAGE）测定变应原各组分的分子质量；应用免疫印迹法检测各组分蛋白质与藜草花粉过敏性哮喘患者血清中sIgG和sIgE的反应率．结果：藜草花粉经电泳后得到12条蛋白质区带，分子质量依次为92，63．1，61，52，45，43，39．7，38．9，34，31．6，28．4和18．5～12ku；免疫印迹可见4条sIgE反应带，蛋白分子质量依次为92，34，31．6和18．5～12ku；与患者血清反应率分别是70％，50％，80％和90％；与血清sIgG结合的反应带有8条，蛋白分子质量为92，61，52，45，43，39．7，31．6和18．5～12ku；与患者血清反应率分别是80％，40％，10％，20％，80％，10％，10％和100％．结论：藜草花粉变应原主要含12种蛋白质成分，其中分子质量为18．5—12ku和92ku的蛋白质变应原活性及免疫原活性均很强，是主要变应原成分；分子质量在34和31．6ku的蛋白质有较强的变应原性而无或仅有较弱的免疫原性；而分子质量为43，61，45，52和39．7ku的蛋白质具有一定的免疫原性而无变应原性．

藜草花粉过敏性哮喘诊断试验的研究

目的比较藜草花粉变应原皮肤挑刺试验（ＳＰＴ）、支气管激发试验（ＢＰＴ）、血清特异性ＩｇＥ（ｓＩｇＥ）的关系，并评估上述试验对藜草花粉过敏性哮喘的诊断价值。方法对４０例秋季哮喘发作的病人同时进行以上试验。结果ＳＰＴ、ｓＩｇＥ与ＢＰＴ有较高的符合率（７０％～８５％）。结论ＳＰＴ、ｓＩｇＥ的诊断价值与ＢＰＴ相仿，ＳＰＴ和ｓＩｇＥ安全、简便，值得临床推广应用。

藜草花粉过敏与支气管哮喘的研究

为了确定藜草花粉过敏原对哮喘患者的致敏作用。对外源性哮喘患者用藜草花粉过敏原进行了皮肤挑刺试验（SPT）和支气管激发试验（BPT）。结果藜草花粉SPT阳性者为28.2％,18例BPT阳性者中,14例出现哮鸣音。藜草花粉BPT与SPT的符合率为85％。提示藜草花粉过敏原是我国哮喘患者的一个重要吸入性过敏原。