[ Objective] To prepare the monoclonal antibody against chicken major histocompatibility complex class II molecules （MHC II）. [ Method ] The prokaryotic expression of the gene fragments of exons 2 -6 encoding alpha ...[ Objective] To prepare the monoclonal antibody against chicken major histocompatibility complex class II molecules （MHC II）. [ Method ] The prokaryotic expression of the gene fragments of exons 2 -6 encoding alpha chain of MHC II and exons 3 -6 encoding beta chain of MHC II were performed based on its protein sequences. After BALB/c mice were immunized with the purified fusion proteins, the mouse spleen cells were fused with mouse myeloma cells SP2/0. Then the positive hybridoma cells were screened and detected by indirect enzyme-linked immunosorbent assay （ELISA）. [ Result] One hybridoma cell strain secreting monoclonal antibody against alpha chain and two strains secreting monoclonal antibody against beta chain were obtained. These three hybridoma cell strains were named as MHC II alpha-4, MHC II betas-2 and MCH II betas-31, respectively. Their titers of ascites in indirect ELISA were 1 ： 256 000, 1 ： 256 000 and 1 ： 1 280 000, respectively. These antibodies could specifically recog- nize MHC II alpha chain or beta chain in western blotting. [ Conclusion] Three obtained hybridoma stains can stably produce the monoclonal antibody against chicken MHC class II molecules.展开更多
因为细胞毒性T淋巴细胞(cytotoxic T lymphocytes,CTLs)在肿瘤控制中起着重要作用,所以基于CTL表位(epitope)的治疗性肿瘤多肽疫苗已经成为当前肿瘤综合生物治疗中一个倍受瞩目的策略。传统的免疫治疗策略通常选择与MHC-Ⅰ类分子具有高...因为细胞毒性T淋巴细胞(cytotoxic T lymphocytes,CTLs)在肿瘤控制中起着重要作用,所以基于CTL表位(epitope)的治疗性肿瘤多肽疫苗已经成为当前肿瘤综合生物治疗中一个倍受瞩目的策略。传统的免疫治疗策略通常选择与MHC-Ⅰ类分子具有高亲和力的CTL表位肽作为治疗工具,但部分高亲和力的CTL表位肽在体内往往有免疫耐受或者副反应。近来研究表明:低亲和力的CTL表位肽及其改构体能很好地克服这些问题。本文就低亲和力CTL表位肽及其改构体在肿瘤免疫治疗中的研究进展做一综述。展开更多
基金supported by the National Natural Science Foundation (30671537)
文摘[ Objective] To prepare the monoclonal antibody against chicken major histocompatibility complex class II molecules （MHC II）. [ Method ] The prokaryotic expression of the gene fragments of exons 2 -6 encoding alpha chain of MHC II and exons 3 -6 encoding beta chain of MHC II were performed based on its protein sequences. After BALB/c mice were immunized with the purified fusion proteins, the mouse spleen cells were fused with mouse myeloma cells SP2/0. Then the positive hybridoma cells were screened and detected by indirect enzyme-linked immunosorbent assay （ELISA）. [ Result] One hybridoma cell strain secreting monoclonal antibody against alpha chain and two strains secreting monoclonal antibody against beta chain were obtained. These three hybridoma cell strains were named as MHC II alpha-4, MHC II betas-2 and MCH II betas-31, respectively. Their titers of ascites in indirect ELISA were 1 ： 256 000, 1 ： 256 000 and 1 ： 1 280 000, respectively. These antibodies could specifically recog- nize MHC II alpha chain or beta chain in western blotting. [ Conclusion] Three obtained hybridoma stains can stably produce the monoclonal antibody against chicken MHC class II molecules.
文摘因为细胞毒性T淋巴细胞(cytotoxic T lymphocytes,CTLs)在肿瘤控制中起着重要作用,所以基于CTL表位(epitope)的治疗性肿瘤多肽疫苗已经成为当前肿瘤综合生物治疗中一个倍受瞩目的策略。传统的免疫治疗策略通常选择与MHC-Ⅰ类分子具有高亲和力的CTL表位肽作为治疗工具,但部分高亲和力的CTL表位肽在体内往往有免疫耐受或者副反应。近来研究表明:低亲和力的CTL表位肽及其改构体能很好地克服这些问题。本文就低亲和力CTL表位肽及其改构体在肿瘤免疫治疗中的研究进展做一综述。
