The Correlation between the Activities of Digestive Enzymes in the Pancreas and Blood Serum in Chicken

It is presently an established fact that pancreatic enzymes (amylase, lipase, and trypsin) can enter the bloodstream as well as the intestine. These enzymes normally circulate within the bloodstream in definite concentration ranges. There is a lack of available information on the interrelationships between the enzymatic activities in pancreatic juice and blood serum in poultry. The study presented was aimed at the determination of possible correlation between the activities of the digestive enzymes in pancreatic juice or pancreatic tissue and in blood serum in chicken. The study was performed on Leghorn cockerels with chronically fistulated main pancreatic duct, and broiler chicks at different ages (14, 24, 28, and 35 days). Activities of all digestive enzymes in pancreatic juice in cockerels were found to grow in 1 hr after the feeding: amylase by 1.8 times, lipase 1.5 times, and proteases 1.3 times compared to basal level (after starving). Activity of trypsin in serum significantly increased by 67.4% in 1 hr after the feeding (P 0.001) while activities of amylase and lipase remained at their respective preprandial levels. The activities of pancreatic enzymes in the pancreas and blood serum in broilers at different ages were positively correlated: r = 0.54 for amylase, r = 0.96 for lipase, and r = 0.99 for trypsin. The strong positive correlation between tryptic activities in the pancreas or pancreatic juice and in blood serum in chicken can be a starting point for further research on the functions of circulatory trypsin which can include the regulation of exocrine pancreatic activity and other vital functions.

Blood and Gastrointestinal Parasites of Chickens and Turkeys Reared in the Tropical Rainforest Zone of Southeastern Nigeria

The blood and gastrointestinal (GI) parasites of chickens and turkeys reared in Owerri, Southeastern Nigeria were studied. Out of 5600 birds, 5040 (90%) were chickens and 560 (10%) were turkeys. Of these birds, 672 (12%) were infected with blood parasites, of which 448 (8.9%) were chickens and 224 (40%) turkeys. The blood parasites found in chickens were Leucocytozoon sp., with a prevalence rate of 448 (8.9%) occurring in both male and female chickens examined. Plasmodium sp. was found in the turkeys and it gave a prevalence of 224 (40%). 1792 (32%) of the birds were infected with faecal parasites, out of which, 1456 (28.9%) were chickens and 336 (60%) turkeys examined. Ascaridia sp. infected 1232 (24.4%) of the chickens and 336 (60%) of the turkeys were examined. The only cestode observed was Raillietina spp., which infected 224 (4.4%) of the chickens, but none in the turkeys. Generally, low blood and faecal infections were observed in the chickens, while infections in the turkeys were slightly higher.

Effects of Imidapril on Venous Blood Gas Values in Broiler Chickens Exposed to Low Ambient Temperature

ObjectiveThis study was designed to evaluate the effects of imidapril on blood gas parameters in broiler chickens. MethodTwenty-four chickens were randomly divided into three groups (n=8), control group, low temperature group and imidapril group. Chickens in low temperature group and imidapril group were exposed to low ambient temperature (12-18 ℃) from age at 14 d to 45 d, whereas the control group was exposed to 24-30 ℃; chickens in imidapril group were gavaged with imidapril (3 mg/kg) once daily for 30 d. At age of 45 d, blood was taken from wing vein and blood gas parameters were evaluated by blood gas analyzer in Luoyang Central Hospital Affiliated to Zhengzhou University. ResultImidapril significantly increased hematocrit (HCT) and total hemoglobin content (T HBC ) and blood Na concentration in broiler chickens exposed to low ambient temperature. No significant differences were observed in pH, P CO 2 , P O 2 , K + , Ca 2+ , HCO 3-, HCO 3std , T CO 2 , BE and SO 2c . ConclusionImidapril increases hematocrit, total hemoglobin content and blood Na + concentration in chickens exposed to low ambient temperature.

破解新开发玉种“没文化”的迷思——兼论新玉种桂林鸡血玉的玉文化开发潜力

随着我国传统玉种资源的逐渐枯竭或被限制开采,在强劲市场需求的拉动下一些新的玉种不断被开采、开发,但新玉种往往面临被认为“没文化”的尴尬局面,影响到新玉种的推广与对产地经济的带动作用。为解决这一迷思造成的困境,本文结合新玉种桂林鸡血玉,提出新玉种的文化发展需要从袭得既有玉文化与转承传统文化、积淀与发展自身玉文化两大途径来实现,而这两大途径的实现效果都受到新玉种自身特性的影响;因此,不同新玉种其玉文化发展的内容丰度、积淀速度是不一样的,并据此分析得出桂林鸡血玉玉文化开发潜力巨大。

响应面法优化冷冻鸡血豆腐的制备工艺

为使肉鸡副产物之一鸡血高效利用。以新鲜鸡血为主要原料,采用低场核磁不易流动水峰面积为评价标准,通过添加不同品种与浓度的抗冻改良剂对冷冻鸡血豆腐中不易流动水峰面积影响的单因素实验,再结合响应面设计优化冷冻鸡血豆腐的制备工艺。进一步以失水率、质构、色泽指标对优化的冷冻鸡血豆腐的品质进行分析,为开发冷冻鸡血豆腐产品提供科学依据。结果表明,冷冻鸡血豆腐优化制备工艺为:添加脂酰乳酸钠0.1%,海藻酸钾0.1%,木薯变性淀粉2%。在此工艺条件下,冷冻鸡血豆腐的低场核磁不易流动水峰面积为2143,与空白组和其他优化组相比不易流动水峰面积最大,故保水效果最好。优化制备的冷冻鸡血豆腐的硬度、咀嚼性、凝胶性以及亮度都显著(P<0.05)高于空白组(其中硬度是空白组的17倍,亮度的是空白组的1.13倍)。因此,冷冻鸡血豆腐的制备工艺可显著提高解冻后鸡血豆腐的品质,可促进肉鸡副产物高值化产品的开发。

昌化“硬地”鸡血石及其相似玉石的矿物学和谱学特征对比研究

随着鸡血石矿产资源的枯竭,市场上出现了种类繁多的鸡血石相似玉石品种,它们的主体矿物组成大多为石英,易与传统的昌化“硬地”鸡血石相混淆。为鉴别和定名此类鸡血石相似玉石,本文利用X射线粉末衍射、红外光谱、拉曼光谱和扫描电子显微镜等测试方法对产自四川、贵州、西安、云南的鸡血石相似玉石样品与昌化“硬地”鸡血石样品的矿物学和光谱学特征进行对比分析。结果表明:(1)X射线粉末衍射显示昌化“硬地”鸡血石样品的主要矿物组成为石英和地开石;不同产地鸡血石相似玉石样品的主要矿物组成为石英和碳酸盐矿物;(2)根据红外光谱的峰形特征可以较快地区分昌化“硬地”鸡血石和鸡血石相似玉石样品,后者在红外光谱常出现1450、881、729 cm^(-1)处的碳酸盐吸收峰,而“硬地”鸡血石样品显示为石英的特征峰位;(3)拉曼光谱结果表明,所有样品均含有石英特征峰,四川、贵州、云南鸡血石相似玉石样品含有方解石的特征拉曼位移,西安样品出现白云石的拉曼位移。“硬地”鸡血石的基质部分含有锐钛矿,而在其他产地的鸡血石相似玉石样品中未见锐钛矿。对于辰砂周围的黄色矿物,“硬地”鸡血石样品是黄铁矿,而贵州样品的黄色部分为雄黄;(4)扫描电子显微镜结果显示“硬地”鸡血石和相似玉石的微形貌特征差异主要集中在碳酸盐矿物,相似玉石样品中的碳酸盐矿物为片状,片与片之间呈叠瓦状堆叠,常见碳酸盐矿物的解理面和阶梯状断口,而“硬地”鸡血石未见此类现象。根据测试结果和国标规定,建议将此类鸡血石相似玉石定名为含辰砂的碳酸盐-石英质玉石。

复合酶制剂对芦花鸡生长性能、屠宰性能及血液生化指标的影响

文章旨在探讨复合酶制剂对芦花鸡生长性能、屠宰性能及血液生化指标的影响。试验将600羽体重相近的42日龄脱温芦花鸡随机分为对照组、复合酶1组和复合酶2组,每组5个重复,每个重复40羽芦花鸡,试验为期78d。结果显示,与对照组相比,芦花鸡42、84和120日龄体重,以及42~120日龄、85~120日龄的ADG、ADFI和F/G均无显著差异(P> 0.05),42~84日龄,复合酶2组的ADG显著高于对照组(P <0.05);复合酶组能提高血液TP、ALB、BUN含量和SOD活性,降低GLU和MDA含量,复合酶2组的TG和TC含量均显著高于对照组(P <0.05),复合酶1组和2组的UA含量分别显著(P <0.05)、极显著(P <0.01)低于对照组;复合酶组屠宰率、全净膛率和半净膛率均有所提高,复合酶组胸肌率和复合酶2组腿肌率均显著高于对照组(P <0.05)。综上所述,重庆山地饲养芦花鸡日粮中添加复合酶制剂可促进芦花鸡生长和蛋白合成,优化脂类代谢,提高鸡体抗氧化能力,提高屠宰性能。

绿茶粉对产蛋后期皖南三黄鸡生产性能、屠宰性能和血液生化指标的影响

本试验旨在探究夏秋茶绿茶粉对产蛋后期皖南三黄鸡产蛋性能、屠宰性能和血液生化指标的影响。将健康状况良好、体重相近的108只62周龄皖南三黄鸡母鸡随机分为对照组(GTP 0)、GTP 0.5组、GTP 1.0组和GTP 1.5组,每组3个重复,每个重复9只。对照组饲喂玉米-豆粕型基础日粮,GTP 0.5组、GTP 1.0组和GTP 1.5组分别饲喂含0.5%、1.0%和1.5%绿茶粉的基础日粮。试验期8周。结果显示:与对照组相比,试验组产蛋率显著降低(P<0.05);GTP 1.0组平均蛋重显著高于对照组(P<0.05),GTP 0.5组和GTP 1.5组平均蛋重和日采食量均低于对照组,而料蛋比则高于对照组(P<0.05);试验组腹脂率随着GTP添加量增加呈降低趋势,其中GTP 1.5组腹脂率显著低于对照组(P<0.05);GTP 0.5组血液总胆固醇(T-CH)含量显著低于对照组(P<0.05);GTP 0.5组血液丙二醛(MDA)含量显著低于对照组(P<0.05),而超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)含量则显著高于对照组(P<0.05)。综上所述,在本试验条件下,添加绿茶粉可以改善产蛋后期皖南三黄鸡的血液生化指标和降低腹脂率,但同时也降低了产蛋性能。

The influences of SE infection on layers' production performance,egg quality and blood biochemical indicators

Background： Salmonella enterica serovar Enteritidis （SE）, as a major cause of foodborn illness, infects humans mainly through the egg. However, the symptom of laying hens usually is not typical and hard to diagnosis. In the present study, it is studied that the influences of SE infection on layers＇ performance, egg quality and blood biochemical indicators. It will help us to improve the strategy to control SE infection in commercial layers. One hundred layers at 20 wk of age were divided into 2 groups, 60 hens for experiment and others for control. The experiment group was fed with the dosage of 108 CFU SE per hen. The specific PCR was used to detect the deposition of SE. On the 8 d after SE infection, 10 hens from the control group and 30 hens from the experimenta group were slaughtered to detect the SE colonization. The production performance, egg quality and blood biochemical indices were also analyzed. Results： The results showed that the colonization rate of SE was highest in caecum contents （55.17%） and lowest in vagina （17.24%）. For the eggs the detection rate of SE was highest on the eggshell （80.00%） and lowest in yolk （18.81%）. SE infection had no significant influence on production performance and egg qualities （P 〉 0.05）. The difference of laying rate between the experimental and control groups was less than 0.30%, and both were approximately equal to 82.00%. The blood analysis showed that the aspartic aminotransferase （AST） and alanine aminotransferase （ALT） of experimental group was significantly higher than those of control group （P 〈 0.05）. For experimental and control groups AST values were 236.22 U/I and 211.84 U/I respectively, and ALT values were 32.19 U/I and 24.55 U/I. All of coefficients were less than 20%. The colonization of SE in organs increases the enzyme activities of AST and ALT in blood. Conclusions： SE in feed could invade the oviduct and infect the forming eggs. It significantly increased the concentration of ALT and AST in blood. However,SE infection was hard to be observed from the appearances of layer and egg. It might be a dangerous risk to human health.

鸡外周血T淋巴细胞分群及免疫相关基因表达分析

为探索鸡抗病育种中简便实用的免疫功能测定指标,试验采用成像流式细胞术对四川山地乌骨鸡和大恒699肉鸡配套系的4周龄和10周龄外周血进行T淋巴细胞亚群分型,并检测外周血中白介素6(IL-6)、Toll样受体3(TLR3)和干扰素(IFN-γ)基因的表达水平。结果显示:10周龄时,CD_(4)^(+)T淋巴细胞亚群在两鸡种间差异显著(P<0.05),4周龄和10周龄的两鸡种CD_(8)^(+)T淋巴细胞亚群均有极显著差异(P<0.01);CD_(4)^(+)/CD_(8)^(+)比值在0.7~1.6之间呈正态分布,且随周龄增加而降低;大恒699肉鸡配套系的CD_(4)^(+)/CD_(8)^(+)比值比四川山地乌骨鸡高;IL-6和IFN-γ表达量随周龄增加而增加,但TLR3相反。细胞因子的增多和CD_(4)^(+)/CD_(8)^(+)比值的降低表明鸡只随周龄增长,机体免疫平衡发生改变。结果表明,基于成像流式细胞术进行鸡外周血T淋巴细胞分型简易可行,CD_(4)^(+)/CD_(8)^(+)比值可作为鸡免疫力检测的一个指标;天然免疫相关基因IL-6、TLR3和IFN-γ基因的表达量可以作为免疫力检测的参考。

“桂林鸡血玉”的色彩、花纹、画面赏析--玉石中的桂林山水

基于作者“桂林鸡血玉”的收藏和资料收集的基础上,从色彩、花纹、画面对丰富多彩的“桂林鸡血玉”进行了整理和分类,分析了“桂林鸡血玉”在视觉美学上的特点,得出:在色彩上,“桂林鸡血玉”是以红色为主色的多色玉种,并形成了以黑红组合为主的多种颜色组合;在花纹上,具有样式丰富性、风格灵动性的特点;在画面上,具有类型多样性、意境艺术性的特点;且在色彩、花纹、画面三者关系上,存在层次递进关系。

鸡血球肽螯合铁的结构表征及其稳定性研究

为了评估鸡血用于膳食铁补充剂的潜力,本文以鸡血和氯化亚铁为原料制备了鸡血球肽螯合铁,采用扫描电镜及差示扫描量热对其结构进行表征,分析了其在不同温度(30~80℃)及pH(2~9)下的稳定性,并对其体外稳定性进行了探讨。结果表明鸡血球肽和铁结合生成一种新的肽铁螯合物。该螯合物具有良好的热稳定性(铁保留率73.76%以上)并耐酸碱。经体外模拟消化发现,鸡血球肽螯合铁的消化稳定性(铁保留率为86.01%)优于硫酸亚铁和葡萄糖酸亚铁。此外,在1%植酸、1%草酸和8%膳食纤维这三种膳食因素的影响下,鸡血球肽螯合铁显示出比硫酸亚铁和葡萄糖酸亚铁更好的生物可及性。

Expression of B Locus Beta 2 (<i>BLB2</i>) Gene at Cytolytic and Latent Immune Response Stages of Immunocompetence in Nigerian Indigenous Chickens

An economically-important trait in poultry for which gene identification continues to be a challenge is immune response. The objective of the study was to quantitate the expression of major histocompatibility complex (MHC) class II BLB2 gene at cytolytic and latent immune response stages in Nigerian indigenous chickens. A total of 108 Nigerian indigenous chickens (NIC) were sourced across the South-western states in Nigeria. The birds were inoculated with sheep red blood cells (SRBC), after which blood samples were obtained (5 days post-inoculation) and antibody haemagglutination test was carried out to place the birds into groups of high and low antibody titre levels. The categorisation of the birds resulted in six groups of normal feather high, normal feather low, naked neck high, naked neck low, frizzle feather high and frizzle feather low antibody groups. A total of 48 chicks were selected from the progeny for gene expression studies. Surgical excision of thymus and spleen was carried out for the detection of cytolytic and latent responses of the birds. β-actin was used as the endogenous control and the critical threshold method (2–ΔΔCт) was carried out for the determination of fold change. The fold change of spleen tissue expression at cytolytic immune response of the birds was 30,362.44 compared to latent response 294.07;and the fold change of thymus expression at cytolytic immune response of the birds was 51.98 compared to latent response 5.24. At both cytolytic and latent stages of immune response to SRBC antigen, BLB2 expression in the spleen was comparatively higher than in the thymus and the height of transcriptional activity was associated with the cytolytic stage. The birds of high titre at both the cytolytic and the latent responses had higher mRNA expression. This study concluded that BLB2 gene expression in the Nigerian indigenous chicken was induced at the cytolytic stage and repressed at the latent stage. During avian infections, the category of high immune response birds would perform better than the low immune response counterpart;and the protective response that BLB2 gene offers will be repressed from one time point to the other.

黄原胶和瓜尔豆胶对鸡血凝胶特性的影响

为改善集中屠宰副产物鸡血的凝胶品质,以保水性、质构、流变特性为评价指标,结合分子作用力和感官评分,研究黄原胶(Xanthan gum,XG)和瓜尔豆胶(Guar gum,GG)的添加量和复配比对鸡血凝胶特性的影响。结果表明:单独添加黄原胶或瓜尔豆胶均能提高鸡血凝胶保水性,黄原胶改善凝胶保水效果更佳,添加4.0 g/L XG的鸡血凝胶蒸煮损失率10.02%、离心损失率12.73%、48 h析水率10.96%,但降低凝胶硬度、黏聚性、胶着性、咀嚼性、回复性。瓜尔豆胶能显著改善凝胶质构特性,添加6.0 g/L GG时可使凝胶达到硬度366.95 N、弹性0.94 mm、黏聚性0.77、胶着性281.94、咀嚼性263.72 mJ、回复性0.25;通过动态流变和分子间作用力分析,鸡血凝胶体系是以弹性响应为主向凝胶化转化;黄原胶和瓜尔豆胶复配对鸡血凝胶具有协同稳定作用,通过增强离子键(从29.18%到32.62%)和氢键(从2.48%到6.43%),减少疏水作用力(从22.68%到16.28%),增加鸡血凝胶的保水性和增强凝胶稳定性。鸡血中添加4.0 g/L的复配胶(黄原胶和瓜尔豆胶配比为7:3)时,鸡血凝胶兼顾保水性、质构特性效果最佳、感官评分高。

低代谢能饲粮添加叶绿素和维生素B_(12)对黄羽肉鸡生长性能、肉品质、血常规指标和养分利用率的影响

本试验旨在研究低代谢能饲粮添加天然植物来源的叶绿素和维生素B_(12)对快速型黄羽肉鸡生长性能、肉品质、血常规指标和养分利用率的影响。试验选用720羽22日龄快速型黄羽肉鸡母鸡,按体重一致原则随机分为4个组,每组6个重复,每个重复30只鸡。对照组饲喂基础饲粮,低代谢能Ⅰ组饲喂基础饲粮代谢能降低0.42 MJ/kg的低代谢能Ⅰ饲粮,低代谢能Ⅰ+叶绿素和维生素B_(12)复合物组饲喂在低代谢能Ⅰ饲粮中添加40 mg/kg叶绿素和0.04 mg/kg维生素B_(12)的饲粮,低代谢能Ⅱ+叶绿素和维生素B_(12)复合物组饲喂在基础饲粮代谢能降低0.84 MJ/kg的低代谢能Ⅱ饲粮中添加40 mg/kg叶绿素和0.04 mg/kg维生素B_(12)的饲粮。试验期28 d,分为22~42日龄和43~49日龄2个阶段。试验鸡地面平养,自由采食和饮水。结果表明:1)与对照组相比,低代谢能Ⅰ组黄羽肉鸡22~49日龄平均日增重和50日龄体重显著降低(P<0.05)。与低代谢能Ⅰ组相比,低代谢能Ⅰ+叶绿素和维生素B_(12)复合组22~49日龄平均日增重和50日龄体重显著提高(P<0.05),且与对照组相比差异不显著(P>0.05)。2)与对照组相比,低代谢能Ⅰ组黄羽肉鸡全净膛率显著降低(P<0.05),而低代谢能Ⅰ+叶绿素和维生素B_(12)复合组和低代谢能Ⅱ+叶绿素和维生素B_(12)复合组全净膛率则无显著差异(P>0.05)。与对照组和低代谢能Ⅰ组相比,低代谢能Ⅰ+叶绿素和维生素B_(12)复合组胸肌45 min pH和24 h红度(a*)值显著提高(P<0.05),胸肌滴水损失显著降低(P<0.05)。3)与对照组和低代谢能Ⅰ组相比,低代谢能Ⅰ+叶绿素和维生素B_(12)复合组黄羽肉鸡血液血红蛋白(HGB)含量和红细胞压积(HCT)显著提高(P<0.05)。与对照组相比,低代谢能Ⅰ+叶绿素和维生素B_(12)复合组红细胞平均血红蛋白浓度(MCHC)显著提高(P<0.05)。4)与低代谢能Ⅰ组相比,低代谢能Ⅰ+叶绿素和维生素B_(12)复合组黄羽肉鸡血浆丙二醛(MDA)含量显著降低(P<0.05)。5)与低代谢能Ⅰ组相比,低代谢能Ⅰ+叶绿素和维生素B_(12)复合组黄羽肉鸡饲粮能量表观利用率显著提高(P<0.05),且与对照组相比无显著差异(P>0.05)。综上所述,在本试验条件下,低代谢能饲粮添加叶绿素和维生素B_(12)可改善黄羽肉鸡生长性能和肉品质,提高饲粮能量表观利用率,达到与基础饲粮一样的饲喂效果。

中草药饲料添加剂对鸡精液品质、生长性能和血液生化指标的影响

文章旨在研究中草药饲料添加剂对鸡精液品质、生长性能和血液生化参数的影响。试验将150只体重相近、健康状况良好的青年鸡随机分为6组,每组5个重复,每个重复5只鸡。对照组饲喂基础日粮,而试验A、B、C、D、E组分别饲喂0.5‰、1.0‰、1.5‰、2‰的中草药饲料添加剂。预饲期10d,正试期60d,试验为期70d。经过多项生物统计分析后,结果表明,在射精量、精子密度、精液pH以及精子畸形率等方面,中草药饲料添加剂组显著优于对照组。血液生理生化指标也表现出明显改善,尤其是在1.0‰添加剂量的组别中表现最为显著。在生长性能指标上,饲喂中草药饲料添加剂的试验组生长性能和饲料转化率显著优于对照组。综上所述,本研究证实了中草药饲料添加剂在提升鸡精液品质,优化血液生理生化指标方面具有积极效应,其中1.0‰的添加剂水平效果最佳。

西畴乌骨鸡与文山土鸡血液生理生化指标的测定

试验旨在为西畴乌骨鸡和文山土鸡的种质保护及认定、人工养殖、饲养管理及疾病防治等提供数据资料。选择成年健康笼养西畴乌骨鸡、笼养文山土鸡各20只(均为公、母各半),测定15项血液生理、生化指标。结果显示:雄性文山土鸡与西畴乌骨鸡的红细胞参数[包括红细胞数(RBC)、血红蛋白(HGB)、血细胞比容(HCT)、平均红细胞体积(MCV)、平均红细胞血红蛋白含量(MCH)]、白细胞参数[包括白细胞数(WBC)、单核细胞数(Mon)、中性粒细胞数(Gran)]、尿酸(UA)含量均极显著高于雌性(P<0.01),雌性文山土鸡与西畴乌骨鸡血液白蛋白(ALB)含量均显著高于雄性(P<0.05)。西畴乌骨鸡WBC极显著高于文山土鸡(P<0.01),雌性西畴乌骨鸡血液葡萄糖(GLU)含量显著高于文山土鸡雌性(P<0.05)。研究表明,根据西畴乌骨鸡与文山土鸡在同品种不同性别以及不同品种间存在的数据差异,应进一步建立基因水平上的差异分析,加强对西畴乌骨鸡与文山土鸡优良性状的筛选及畜禽资源的保护。

颈神经不同支配区合谷刺治疗神经根型颈椎病(气滞血瘀证)的临床研究

目的观察颈神经不同支配区合谷刺治疗神经根型颈椎病(气滞血瘀证)的临床疗效。方法64例神经根型颈椎病(气滞血瘀证)患者纳入最终统计,观察组与对照组各32例。两组均根据颈神经支配区加减配穴,对照组采用常规针刺,观察组采用合谷刺法。两组分别于治疗前、初次治疗后、治疗2疗程、4疗程评估简式McGill疼痛量表、颈痛量表(NPQ)及中医证候积分,于治疗结束后1个月随访,并比较两组的临床疗效。结果初次治疗后对照组PRI、VAS、SF-MPQ评分较治疗前明显降低,观察组PRI、VAS、PPI、SF-MPQ评分较治疗前明显降低,且观察组VAS评分改善优于对照组(P<0.05)。治疗2疗程、4疗程、随访时,两组PRI、VAS、PPI、SF-MPQ、NPQ、中医证候积分较治疗前均降低(P<0.05);观察组治疗2疗程时PRI、VAS、PPI、SF-MPQ、NPQ、中医证候积分均低于对照组(P<0.05);治疗4疗程及随访时,PPI评分组间差异无统计学意义(P>0.05),PRI、VAS、SF-MPQ、NPQ、中医证候积分观察组均低于对照组(P<0.05);观察组愈显率显著高于对照组(P<0.05);观察组总有效率高于对照组。结论合谷刺立时止痛效果优于常规针刺,在缓解神经肌肉不适症状,改善疼痛及相关日常功能障碍、心理障碍等方面具有优势。

雏鸡淋巴细胞变化规律的流式细胞仪检测研究

为进一步研究鸡在出孵1个月左右时间内T、B淋巴细胞及其亚群的发育状况,将SPF雏鸡分别在3、5、7、9、11、14、17、22、27、32日龄时取血液、脾脏、法氏囊及胸腺,分离并制成淋巴细胞悬液,流式细胞仪双染色法检测Bu-1a+B淋巴细胞、CD3+、CD4+、CD8+和TCRγδ+CD3+T淋巴细胞含量及血液淋巴细胞的凋亡规律.结果:在出孵1个月内T、B细胞处于分化发育阶段的初期,除胸腺外,呈上升趋势,但T细胞亚群的变化无规律,B细胞在法氏囊中和T细胞在脾脏中3～9日龄发育最快;血液淋巴细胞凋亡趋于下降,尤其是在9日龄以后.此间不断有成熟的淋巴细胞产生,不断进入血液和脾脏,使其数量有上升趋势.但是,血液中第7日龄时Bu-1a+B淋巴细胞和CD3+、CD4+、CD8+T淋巴细胞数量均最低,说明体液免疫和细胞免疫水平第7日龄时都最低,并在17日龄时进入水平较高的又一个低谷.TCRγδ+CD3+T细胞则变化幅度不大.

蛋鸡品系RAPD变异及其与杂种优势关系的分析

为探讨RAPD标记在家鸡杂种优势预测中的应用价值，分析了力康自壳蛋鸡纯系的RAPD变异及其与杂种优势的关系．5个纯系内和系间显示出低水平的变异．根据品系间遗传距离DS用UPGMA法进行聚类分析，品系a、e、d聚为一类，而h、l聚为另一类．简单线性相关分析表明，父系相似系数与F1代的60周龄产蛋数存在正相关而与蛋重的杂种优势率存在负相关；母系相似系数与产蛋数的杂种优势率存在高度负相关．但品系间遗传距离与各性状及其杂种优势率之间没有显著的相关关系．