[ Objective] The paper presents the effects of Chinese herbal extract on the performance of local chickens for its application in animal production. [Method] The herbal extract Ⅰ and Ⅱ were added to the basal diet o...[ Objective] The paper presents the effects of Chinese herbal extract on the performance of local chickens for its application in animal production. [Method] The herbal extract Ⅰ and Ⅱ were added to the basal diet of 10 day-old chickens at the ratio of 0.6%, respectively. Then the chickens was fasted and respectively weighed at 0, 17, 24 and 31 days old. And the feed consumption and mortality of each group were recorded to determine average weight, average daily gain, and feed conversion ratio. Economic benefit was also analyzed. [ Result] There was no significant difference in average daily gain between the test groups and the control group as well as between the two test groups （P〉0.05） ; there was signifi- cant difference in feed conversion ratio between the test groups and the control group （ P 〈0.05）, but no significant difference between the two test groups （ P 〉 0.05） ; the survival rate and the economic benefits of the test groups were significantly higher than that of control （ P 〈 0.05）. [ Conclusion ] Chinese herbal extract Ⅰ and Ⅱ should improve chicken average daily gain, feed conversion ratio and survival rate, promote growth, and signifi- cantly improve economic benefit.展开更多
[Objective] This trial was to investigate the effect of dietary Picria felterrae Lour extracts on serum biochemical indexes and immune function of Guangxi Partridge chickens. [Method] Totally 500 individuals of 1-day-...[Objective] This trial was to investigate the effect of dietary Picria felterrae Lour extracts on serum biochemical indexes and immune function of Guangxi Partridge chickens. [Method] Totally 500 individuals of 1-day-old Guangxi Partridge chickens were randomly divided into five groups, five replicates each group and 20 chicks each replicate. Groups A, B and C were treatment groups supplemented with 0.70%, 0.35% and0.175% P. felterrae extracts in the basal diet, respectively; group D was a medical control group supplemented with 0.01% colistin sulfate premix in the basal diet; and group E was a control group with basal diet. Serum samples were collected from 10 chickens in each group at 21, 35 and 49 days of age, to analyze the biochemical and immune indexes. [Result] It had no significant impact on serum TP and GLU contents of chickens by adding different levels of P. felterrae extracts in the basal diet（P 〉0.05）. However, at 21 and 35 days of age, the TBILI content of chickens in groups A, B and C were extremely lower or significantly lower than that in group E（ P〈0.01; P〈0.05）. At 35 days of age, the serum AKP activities in groups A, B and C were extremely higher than that in group E（P〈0.01）, and the serum GOT activities at 21 and 35 days of age in group C were significantly higher than that in group E（P〈0.05）. At 49 days of age, the serum Ig G content in groups A, B and C and the IL-2 content in group B and group C were significantly lower or extremely lower than that in group E（ P〈0.05; P〈0.01）; the T-AOC content in group B was significantly higher than that in group E（P〈0.05）; the GSH content in groups A, B and C were significantly higher than that in group E（ P〈0.05）. Adding different levels of P. felterrae extracts in the diet significantly decreased the TBILI content, reduced the Ig G, IL-2 and IL-6 contents, and improved the AKP and GOT activities and the T-AOC and GSH contents of Guangxi Partridge chicken. [Conclusion] Adding 0.35% P. felterrae extracts in the basal diet received the best effort.展开更多
Chicken manures added with Cu and Zn were used for composting experiment to study the dynamics of different soluble humus complexed copper （H2O-Cu,NaOH-Cu and NaOH-Na4P2O7-Cu）.The effect of zinc in compost on humus ...Chicken manures added with Cu and Zn were used for composting experiment to study the dynamics of different soluble humus complexed copper （H2O-Cu,NaOH-Cu and NaOH-Na4P2O7-Cu）.The effect of zinc in compost on humus complexed copper and the relationship between humus complexed copper were also revealed.The results showed that H2O-Cu increased before the 45 d of composting and decreased thereafter.In the treatments of 1：1,2：1 and 3：1 with the added copper content increasing,H2O-Cu increased with decomposition process.Added high level zinc in manure could decrease the relative H2O-Cu content at the last stage of composting.NaOH-Cu decreased greatly with the compost proceeding.With the added copper content increasing in manure,the percentage of NaOH-Cu to total copper decreased.Added zinc had no significant impact on NaOH-Cu concentration.NaOH-Na4P2O7-Cu increased with composting.With the added copper content increasing in compost manure,the percentage of NaOH-Na4P2O7-Cu to total copper increased,and showed an increase trend with composting proceeding.Added zinc in manure increased NaOH-Na4P2O7-Cu.In composts,55-80% of extracted copper was present in NaOH fraction,10-30% in NaOH-Na4P2O7-Cu fraction,and 0-10% in H2O fraction.Whatever the added copper or zinc contents increased in manures,the distribution percent of NaOH- Na4P2O7-Cu （pressed as percent of extracted Cu） increased and that of H2O-Cu and NaOH-Cu decreased.Three extracted Cu （H2O,NaOH and Na4P2O7） were strongly correlated to each other,H2O-Cu and NaOH-Na4P2O7-Cu were significantly correlated to H2O-extracted organic C and NaOH-Na4P2O7-extracted organic C,respectively.展开更多
建立了固相萃取-超高效液相色谱-串联质谱法(SPE-UPLC-MS/MS)快速测定鸡粪中37种抗菌药物含量的方法。鸡粪经冻干制备,80%乙腈(含0.1%甲酸)提取,Oasis PRiME HLB通过式固相萃取净化后,利用UPLC-MS/MS测定抗菌药物含量。结果表明,37种抗...建立了固相萃取-超高效液相色谱-串联质谱法(SPE-UPLC-MS/MS)快速测定鸡粪中37种抗菌药物含量的方法。鸡粪经冻干制备,80%乙腈(含0.1%甲酸)提取,Oasis PRiME HLB通过式固相萃取净化后,利用UPLC-MS/MS测定抗菌药物含量。结果表明,37种抗菌药物浓度在0.40~200.0ng/mL范围内线性关系良好,相关系数(R^(2))大于0.996,检出限(LOD)为1μg/kg,定量限(LOQ)为4μg/kg。37种抗菌药物在3个浓度添加标水平(LOQ、5倍LOQ、10倍LOQ)下的回收率为63.5%~104.2%,批内相对标准偏差为1.47%~10.5%,批间相对标准偏差为2.39%~14.2%。所建立方法准确、快速、灵敏、稳定,可实现鸡粪中37种抗菌药物的同时快速测定。展开更多
文摘[ Objective] The paper presents the effects of Chinese herbal extract on the performance of local chickens for its application in animal production. [Method] The herbal extract Ⅰ and Ⅱ were added to the basal diet of 10 day-old chickens at the ratio of 0.6%, respectively. Then the chickens was fasted and respectively weighed at 0, 17, 24 and 31 days old. And the feed consumption and mortality of each group were recorded to determine average weight, average daily gain, and feed conversion ratio. Economic benefit was also analyzed. [ Result] There was no significant difference in average daily gain between the test groups and the control group as well as between the two test groups （P〉0.05） ; there was signifi- cant difference in feed conversion ratio between the test groups and the control group （ P 〈0.05）, but no significant difference between the two test groups （ P 〉 0.05） ; the survival rate and the economic benefits of the test groups were significantly higher than that of control （ P 〈 0.05）. [ Conclusion ] Chinese herbal extract Ⅰ and Ⅱ should improve chicken average daily gain, feed conversion ratio and survival rate, promote growth, and signifi- cantly improve economic benefit.
基金Supported by Science and Technology Development Program of Nanning City(20155181)Key Technology Research and Development Program of Guangxi Province(14121003-3-3)
文摘[Objective] This trial was to investigate the effect of dietary Picria felterrae Lour extracts on serum biochemical indexes and immune function of Guangxi Partridge chickens. [Method] Totally 500 individuals of 1-day-old Guangxi Partridge chickens were randomly divided into five groups, five replicates each group and 20 chicks each replicate. Groups A, B and C were treatment groups supplemented with 0.70%, 0.35% and0.175% P. felterrae extracts in the basal diet, respectively; group D was a medical control group supplemented with 0.01% colistin sulfate premix in the basal diet; and group E was a control group with basal diet. Serum samples were collected from 10 chickens in each group at 21, 35 and 49 days of age, to analyze the biochemical and immune indexes. [Result] It had no significant impact on serum TP and GLU contents of chickens by adding different levels of P. felterrae extracts in the basal diet（P 〉0.05）. However, at 21 and 35 days of age, the TBILI content of chickens in groups A, B and C were extremely lower or significantly lower than that in group E（ P〈0.01; P〈0.05）. At 35 days of age, the serum AKP activities in groups A, B and C were extremely higher than that in group E（P〈0.01）, and the serum GOT activities at 21 and 35 days of age in group C were significantly higher than that in group E（P〈0.05）. At 49 days of age, the serum Ig G content in groups A, B and C and the IL-2 content in group B and group C were significantly lower or extremely lower than that in group E（ P〈0.05; P〈0.01）; the T-AOC content in group B was significantly higher than that in group E（P〈0.05）; the GSH content in groups A, B and C were significantly higher than that in group E（ P〈0.05）. Adding different levels of P. felterrae extracts in the diet significantly decreased the TBILI content, reduced the Ig G, IL-2 and IL-6 contents, and improved the AKP and GOT activities and the T-AOC and GSH contents of Guangxi Partridge chicken. [Conclusion] Adding 0.35% P. felterrae extracts in the basal diet received the best effort.
基金supported by the National Natural Science Foundation of China (39800093)the Youth Project of Northwest A&F University, China+1 种基金the Innovative Project for University Students of Ministry of Education of Chinathe Key Technologies R&D Program of China during the 11th Five-Year Plan period (2007BAD89B16)
文摘Chicken manures added with Cu and Zn were used for composting experiment to study the dynamics of different soluble humus complexed copper （H2O-Cu,NaOH-Cu and NaOH-Na4P2O7-Cu）.The effect of zinc in compost on humus complexed copper and the relationship between humus complexed copper were also revealed.The results showed that H2O-Cu increased before the 45 d of composting and decreased thereafter.In the treatments of 1：1,2：1 and 3：1 with the added copper content increasing,H2O-Cu increased with decomposition process.Added high level zinc in manure could decrease the relative H2O-Cu content at the last stage of composting.NaOH-Cu decreased greatly with the compost proceeding.With the added copper content increasing in manure,the percentage of NaOH-Cu to total copper decreased.Added zinc had no significant impact on NaOH-Cu concentration.NaOH-Na4P2O7-Cu increased with composting.With the added copper content increasing in compost manure,the percentage of NaOH-Na4P2O7-Cu to total copper increased,and showed an increase trend with composting proceeding.Added zinc in manure increased NaOH-Na4P2O7-Cu.In composts,55-80% of extracted copper was present in NaOH fraction,10-30% in NaOH-Na4P2O7-Cu fraction,and 0-10% in H2O fraction.Whatever the added copper or zinc contents increased in manures,the distribution percent of NaOH- Na4P2O7-Cu （pressed as percent of extracted Cu） increased and that of H2O-Cu and NaOH-Cu decreased.Three extracted Cu （H2O,NaOH and Na4P2O7） were strongly correlated to each other,H2O-Cu and NaOH-Na4P2O7-Cu were significantly correlated to H2O-extracted organic C and NaOH-Na4P2O7-extracted organic C,respectively.
文摘建立了固相萃取-超高效液相色谱-串联质谱法(SPE-UPLC-MS/MS)快速测定鸡粪中37种抗菌药物含量的方法。鸡粪经冻干制备,80%乙腈(含0.1%甲酸)提取,Oasis PRiME HLB通过式固相萃取净化后,利用UPLC-MS/MS测定抗菌药物含量。结果表明,37种抗菌药物浓度在0.40~200.0ng/mL范围内线性关系良好,相关系数(R^(2))大于0.996,检出限(LOD)为1μg/kg,定量限(LOQ)为4μg/kg。37种抗菌药物在3个浓度添加标水平(LOQ、5倍LOQ、10倍LOQ)下的回收率为63.5%~104.2%,批内相对标准偏差为1.47%~10.5%,批间相对标准偏差为2.39%~14.2%。所建立方法准确、快速、灵敏、稳定,可实现鸡粪中37种抗菌药物的同时快速测定。
