[Objectives]The paper was to study the effects of combined application of nonreplicating Toxoplasma uracil auxotrophs(NRTUAs)and Agaricus blazei Murill polysaccharide(ABP)on growth and humoral immunity of chicks.[Meth...[Objectives]The paper was to study the effects of combined application of nonreplicating Toxoplasma uracil auxotrophs(NRTUAs)and Agaricus blazei Murill polysaccharide(ABP)on growth and humoral immunity of chicks.[Methods]A total of 120 one-day old female Hyline brown laying hens were randomly divided into 4 groups,30 hens for each group.The chicks in group 1 were subcutaneously injected with NRTUAs and fed on the diet containing with ABP;the chicks in group 2 were subcutaneously injected with NRTUAs;the chicks in group 3 were subcutaneously injected with equal volume of PBS,and fed on the diet containing with ABP;the chicks in group 4 were subcutaneously injected with equal volume of PBS.The body weight of chicks in each group was counted at the 21^(st),42^(nd),84^(th)and 112^(th)week.During this period,blood samples were collected from chicks in each group at 0,7,14,21,28 and 35 d post immunization against Newcastle disease(ND),and serum was separated to detect the antibody titer of ND.[Results]The combined application of NRTUAs and ABP had no effect on growth of chicks,but promoted the humoral immune response of chicks,significantly improved the ND antibody level of chicks,and could maintain high levels of antibodies in the body for a long time.[Conclusions]The study lays a theoretical foundation for further developing the clinical application of NRTUAs and ABP.展开更多
day-old AA broilers were conducted with violent Marek′s disease virus (vMDV). The activity of SOD, Se-GSHPx, content of LPO in central, peripheral immune organs and main visceral organs of chicks were detected at 7, ...day-old AA broilers were conducted with violent Marek′s disease virus (vMDV). The activity of SOD, Se-GSHPx, content of LPO in central, peripheral immune organs and main visceral organs of chicks were detected at 7, 14, 28, 42, and 56 days after infection with vMDV, respectively, and chemilunescence (CL) assay was adopted to detect the ability of active oxygen radical (AOR) generation of lymphocytes from thymus, spleen and bursa of Fabricious. The results showed: (1) the activity of total SOD (T-SOD) and Mn-SOD of vMDV-infected chicks decreased significantly (P<0.05, P<0.01) compared to control group, whereas that of Cu, Zn-SOD increased slightly (P>0.05); (2) the activity of Se-GSHPx decreased significantly (P<0.05, P<0.01); (3) the content of LPO increased significantly (P<0.05, P<0.01); (4) the value of CL of lymphocytes increased significantly (P<0.05, P<0.01. These showed that vMDV infection resulted in increasing of generation of AOR and decreasing of activity of AOR scavenging enzyme system, leading to deposition of AOR, intensive lipid peroxidation and conversion of cells in chicks. Which gave a further proof that dynamic changes of detected items were consistent with action of vMDV. These proved that AOR resulting from vMDV infection mediated Marek′s disease (MD) and MD oncoma.展开更多
基金Supported by National Natural Science Foundation of China(31902277)。
文摘[Objectives]The paper was to study the effects of combined application of nonreplicating Toxoplasma uracil auxotrophs(NRTUAs)and Agaricus blazei Murill polysaccharide(ABP)on growth and humoral immunity of chicks.[Methods]A total of 120 one-day old female Hyline brown laying hens were randomly divided into 4 groups,30 hens for each group.The chicks in group 1 were subcutaneously injected with NRTUAs and fed on the diet containing with ABP;the chicks in group 2 were subcutaneously injected with NRTUAs;the chicks in group 3 were subcutaneously injected with equal volume of PBS,and fed on the diet containing with ABP;the chicks in group 4 were subcutaneously injected with equal volume of PBS.The body weight of chicks in each group was counted at the 21^(st),42^(nd),84^(th)and 112^(th)week.During this period,blood samples were collected from chicks in each group at 0,7,14,21,28 and 35 d post immunization against Newcastle disease(ND),and serum was separated to detect the antibody titer of ND.[Results]The combined application of NRTUAs and ABP had no effect on growth of chicks,but promoted the humoral immune response of chicks,significantly improved the ND antibody level of chicks,and could maintain high levels of antibodies in the body for a long time.[Conclusions]The study lays a theoretical foundation for further developing the clinical application of NRTUAs and ABP.
文摘day-old AA broilers were conducted with violent Marek′s disease virus (vMDV). The activity of SOD, Se-GSHPx, content of LPO in central, peripheral immune organs and main visceral organs of chicks were detected at 7, 14, 28, 42, and 56 days after infection with vMDV, respectively, and chemilunescence (CL) assay was adopted to detect the ability of active oxygen radical (AOR) generation of lymphocytes from thymus, spleen and bursa of Fabricious. The results showed: (1) the activity of total SOD (T-SOD) and Mn-SOD of vMDV-infected chicks decreased significantly (P<0.05, P<0.01) compared to control group, whereas that of Cu, Zn-SOD increased slightly (P>0.05); (2) the activity of Se-GSHPx decreased significantly (P<0.05, P<0.01); (3) the content of LPO increased significantly (P<0.05, P<0.01); (4) the value of CL of lymphocytes increased significantly (P<0.05, P<0.01. These showed that vMDV infection resulted in increasing of generation of AOR and decreasing of activity of AOR scavenging enzyme system, leading to deposition of AOR, intensive lipid peroxidation and conversion of cells in chicks. Which gave a further proof that dynamic changes of detected items were consistent with action of vMDV. These proved that AOR resulting from vMDV infection mediated Marek′s disease (MD) and MD oncoma.
