Aim To investigate the structure of mogroside Ⅳa isolated from traditionalChinese medicine fructus mo-mordicae [fruits of Siraitia grosvenori (Swingle) C. Jeffery] andsummarize the NMR characteristics of the structur...Aim To investigate the structure of mogroside Ⅳa isolated from traditionalChinese medicine fructus mo-mordicae [fruits of Siraitia grosvenori (Swingle) C. Jeffery] andsummarize the NMR characteristics of the structure. Methods Common extraction, separation andpurification methods were used. Various NMR techniques including ~1H NMR, ^(13)C NMR, DEPT, ~1H-~1HCOSY, HSQC, HMBC, NOESY and molecular model simulated by computer were used to elucidate thestructure. Results ~1H and ^(13)C NMR signals of mogroside Ⅳa were assigned, and spectroscopicbasis was obtained for identification of such type of compounds. Conclusion 1D and 2D NMR techniquesincluding ~1H-~1H COSY, HSQC, HMBC, NOESY spectra are powerful tools for structure analysis. Thestructure determined by NMR methods is identical with energy minimized conformation simulated bycomputer.展开更多
为评价动植物源天然产物的二肽基肽酶-Ⅳ(dipeptidyl peptidase-Ⅳ,DPP-Ⅳ)抑制活性,以苦瓜皂苷(momordica saponins,MS)、乳清蛋白胃蛋白酶水解物(whey protein pepsin hydrolysates,WPPHs)、乳清蛋白胰蛋白酶水解物(whey protein tryp...为评价动植物源天然产物的二肽基肽酶-Ⅳ(dipeptidyl peptidase-Ⅳ,DPP-Ⅳ)抑制活性,以苦瓜皂苷(momordica saponins,MS)、乳清蛋白胃蛋白酶水解物(whey protein pepsin hydrolysates,WPPHs)、乳清蛋白胰蛋白酶水解物(whey protein trypsin hydrolysates,WPTHs)为原料,采用单因素试验和响应面分析优化水解条件,再将MS与WPPHs、WPTH进行复配,结合模拟体外消化,探究DPP-Ⅳ抑制活性。结果表明,MS、WPPHs及WPTHs具有DPP-Ⅳ抑制活性,最佳水解条件为:当酶添加量4%、pH2.7酶解2h时,WPPHs的DPP-Ⅳ抑制率最高为15.43%;当酶添加量4%、pH 7.6酶解4 h时,WPTHs的DPP-Ⅳ抑制率最高为14.62%。苦瓜皂苷与乳清蛋白水解物具有协同对二肽基肽酶-Ⅳ抑制活性作用,当MS与WPPHs、WPTHs的复配体积比均为1:1时,显著高于两者单独累加(p<0.05)。经胃消化后,WPPHs的DPP-Ⅳ抑制率降低2.02%,MS和WPTHs的DPP-Ⅳ抑制率升高0.99%、7.01%;经肠道消化后,WPPHs的DPP-Ⅳ抑制率升高2.23%,而MS和WPTHs的DPP-Ⅳ抑制率降低4.12%、2.70%。研究结果可为天然动植物源产物协同对二肽基肽酶-Ⅳ抑制活性提供了基础性数据,为降血糖功能性产品的开发提供新的方向。展开更多
目的:建立新型有效组份制剂红鹤胶囊多指标成分体外溶出度的测定方法并评价其溶出度。方法:依据2010年版《中华人民共和国药典》,以900 m L的0.3%十二烷基硫酸钠溶液为溶出介质,采用桨法测定,转速为100 r/min,温度为(37±0.5)℃。...目的:建立新型有效组份制剂红鹤胶囊多指标成分体外溶出度的测定方法并评价其溶出度。方法:依据2010年版《中华人民共和国药典》,以900 m L的0.3%十二烷基硫酸钠溶液为溶出介质,采用桨法测定,转速为100 r/min,温度为(37±0.5)℃。实验以竹节参皂苷Ⅳa、总皂苷、总黄酮为检测指标,分别采用高效液相法和紫外分光光度法检测红鹤胶囊中竹节参皂苷Ⅳa、总皂苷和总黄酮的溶出含量。结果:红鹤胶囊的三批样品,在溶出时间为45 min时,竹节参皂苷Ⅳa的累积溶出率分别为91.29%、88.64%、91.72%;总皂苷的累积溶出率分别为89.41%、87.68%、87.10%;总黄酮的累积溶出率分别为91.02%、90.77%、89.98%。结论:经过溶出条件的系统筛选后所建立的溶出度测定方法稳定,结果可靠。红鹤胶囊中各指标性成分溶出度均符合药典规定。展开更多
文摘Aim To investigate the structure of mogroside Ⅳa isolated from traditionalChinese medicine fructus mo-mordicae [fruits of Siraitia grosvenori (Swingle) C. Jeffery] andsummarize the NMR characteristics of the structure. Methods Common extraction, separation andpurification methods were used. Various NMR techniques including ~1H NMR, ^(13)C NMR, DEPT, ~1H-~1HCOSY, HSQC, HMBC, NOESY and molecular model simulated by computer were used to elucidate thestructure. Results ~1H and ^(13)C NMR signals of mogroside Ⅳa were assigned, and spectroscopicbasis was obtained for identification of such type of compounds. Conclusion 1D and 2D NMR techniquesincluding ~1H-~1H COSY, HSQC, HMBC, NOESY spectra are powerful tools for structure analysis. Thestructure determined by NMR methods is identical with energy minimized conformation simulated bycomputer.
文摘目的:建立新型有效组份制剂红鹤胶囊多指标成分体外溶出度的测定方法并评价其溶出度。方法:依据2010年版《中华人民共和国药典》,以900 m L的0.3%十二烷基硫酸钠溶液为溶出介质,采用桨法测定,转速为100 r/min,温度为(37±0.5)℃。实验以竹节参皂苷Ⅳa、总皂苷、总黄酮为检测指标,分别采用高效液相法和紫外分光光度法检测红鹤胶囊中竹节参皂苷Ⅳa、总皂苷和总黄酮的溶出含量。结果:红鹤胶囊的三批样品,在溶出时间为45 min时,竹节参皂苷Ⅳa的累积溶出率分别为91.29%、88.64%、91.72%;总皂苷的累积溶出率分别为89.41%、87.68%、87.10%;总黄酮的累积溶出率分别为91.02%、90.77%、89.98%。结论:经过溶出条件的系统筛选后所建立的溶出度测定方法稳定,结果可靠。红鹤胶囊中各指标性成分溶出度均符合药典规定。
