Background:Benign prostatic hyperplasia(BPH)is a common condition in middle-aged and elderly men.Enlargement of the prostate causes lower urinary tract symptoms.Capsaicin is a phytochemical extracted from chili pepper...Background:Benign prostatic hyperplasia(BPH)is a common condition in middle-aged and elderly men.Enlargement of the prostate causes lower urinary tract symptoms.Capsaicin is a phytochemical extracted from chili peppers and exerts many pharmacological actions,such as anti-tumor and anti-inflammatory effects.Methods:Our study investigated the effect of capsaicin in vitro and in a mouse model in vivo.A prostatic stromal myofibroblast cell line(WPMY-1)was co-incubated with testosterone(1µM)and different concentrations of capsaicin(10–100µM)for 24 and 48 h.Capsaicin(10–100µM)significantly inhibited testosterone-treated WPMY-1 cell growth at 48 h by MTT assay.The testosterone propionate(7.5 mg/kg)-induced BPH mouse model was used to examine the anti-proliferative effect of capsaicin.Treatment with capsaicin(10 mg/kg)for 14 days significantly attenuated prostatic hyperplasia.Finasteride was used as a positive control.Results:Capsaicin significantly decreased prostate weight and prostate index(prostate/body weight ratio)in BPH mice.The expression of 5α-reductase type II,androgen receptor(AR)and prostate specific antigen(PSA)protein expression and PSA serum were all significantly reduced in capsaicin-treated BPH mice.In addition,capsaicin also activated transient receptor potential vanilloid 1 mediated apoptosis and autophagy in BPH mice.Conclusion:These results demonstrate multiple positive effects of capsaicin in controlling prostate growth and suggest its therapeutic potential in the treatment of BPH.展开更多
[Objectives]This study was conducted to investigate the effects of Polygonatum odoratum polysaccharides on the quality of white chili peppers in jars.[Methods]White chili peppers were pickled by adding different conce...[Objectives]This study was conducted to investigate the effects of Polygonatum odoratum polysaccharides on the quality of white chili peppers in jars.[Methods]White chili peppers were pickled by adding different concentrations of P.odoratum polysaccharides with traditional pickling technology,and its nitrite content,pH and sensory quality were analyzed and detected.[Results]The results showed that,compared with the control group without adding P.odoratum polysaccharides,the nitrite content in white chili peppers in jars decreased after adding P.odoratum polysaccharides.When the addition amount of P.odoratum polysaccharides was 0.002%,the inhibition rate of nitrite reached the maximum.Under the same fermentation time,P.odoratum polysaccharides could significantly reduce the pH value of pickles.When the addition amount of P.odoratum polysaccharide was 0.002%and the pickling time was 3 months,the sensory quality of white chili peppers in jars was the best.At this point,white chili peppers in jars had rich aroma and tasted soft and sour,and the sour and salinity were suitable.As the fermentation time continued to increase,the chili aroma of white chili peppers in jars gradually decreased,and the sour taste became more pronounced.[Conclusions]This study can provide reference for the safety and quality control of white chili peppers in jars.展开更多
Chili sauce shrimp paste (CSSP) is an exotic traditional sauce prepared using mainly fresh chili and shrimp paste well known as sambal terasi (Indonesia) and sambal belacan (Malaysia and Brunei). This study aims to ev...Chili sauce shrimp paste (CSSP) is an exotic traditional sauce prepared using mainly fresh chili and shrimp paste well known as sambal terasi (Indonesia) and sambal belacan (Malaysia and Brunei). This study aims to evaluate CSSP from different regions in Indonesia on physiochemical properties, volatile compounds, and sensory evaluation. Evaluation in free amino acid (FAA) and volatile compounds were analyzed using HPLC and GC-MS. CSSP had no significant (p > 0.05) on proximate (except for moisture), salinity, pH, microbial counts and the redness index (a*/b*) compared to control. The identified volatiles can be grouped into hydrocarbon, carbonyl, sulfur, alcohol, nitrogen, fatty acid, and ester compounds. The CSSP A, B, C, D, and E (control) contained 9, 21, 12, 29, and 6 volatile compounds respectively. The total FAA ranged from 44.32 to 67.03 g/100 g, and histidine (his) was found as the most abundant in each CSSP. The rheological properties (yield stress, viscosity, and consistency) of CSSP and control values were significantly, except for CSSP B. The intensities perceived of saltiness, sweetness, and bitterness were greatly varied among CSSP. Although there is no correlation between sweetness and bitterness this is indicated by the negative correlation.展开更多
Azoxystrobin acts as an inhibitor of electron transport by binding to the Qo center of cytochrome b (cyt b). Resistance to azoxystrobin was usually caused by the point mutation of cyt b gene or by the induction of a...Azoxystrobin acts as an inhibitor of electron transport by binding to the Qo center of cytochrome b (cyt b). Resistance to azoxystrobin was usually caused by the point mutation of cyt b gene or by the induction of alternative respiration. Oxygen consumption test for mycelia of Colletotrichum capsici showed that azoxystrobin inhibited mycelial respiration within 12 h; however, as time went on, the respiration of the mycelia recovered when the mycelia were treated with azoxystrobin and salicylhydroxamic acid (SHAM, a known inhibitor of alternative respiration), and the oxygen consumption of the mycelia could not be inhibited. Meanwhile, cytochrome b (cyt b) gene expression increased with the recovery of mycelial respiration. The increased cyt b gene expression might play a role in the development of resistance to azoxystrobin in C. capsici.展开更多
The entire fermentation process of traditional Chinese broad bean paste with chili comprises three individual stages:Tianbanzi,chili pei,and paste fermentation(Tianbanzi-chili pei mixture).Three stages share average 7...The entire fermentation process of traditional Chinese broad bean paste with chili comprises three individual stages:Tianbanzi,chili pei,and paste fermentation(Tianbanzi-chili pei mixture).Three stages share average 77.53%of all bacteria(89 genera),indicating that the similar environment leads to the similar bacterial communities.One,one,and three genera are exclusive to Tianbanzi,chili pei,and paste stages,respectively,due to the special physical and chemical properties for each stage.Total acidity,pH,and NaCl are important endogenous factors that promote the succession of bacterial communities.According to the dynamics of organic acids,reducing sugars,amino acids,and volatile compounds,60-,210-,and 180-day are considered the best fermentation periods for Tianbanzi,chili pei,and paste,respectively,to balance time cost and product quality.Three(Tetragenococcus,Lactobacillus,and Pseudomonas),four(Tetragenococcus,Lactobacillus,Bacillus,and Pseudomonas),and five(Tetragenococcus,Lactobacillus,Bacillus,Pseudomonas,and Pediococcus)genera are considered the core functional bacteria of Tianbanzi,chili pei,and paste fermentation,respectively.展开更多
Sudan dyes are synthetic azo dyes which are widely used in industry. Although they are not allowed in foodstuffs, they have been found contaminating in different food products and their presence is regularly reported....Sudan dyes are synthetic azo dyes which are widely used in industry. Although they are not allowed in foodstuffs, they have been found contaminating in different food products and their presence is regularly reported. It is assumed that these appearances are due to cross-contamination or adulteration. In this paper, we present a newly developed fast and sensitive method for the quantification of eight Sudan dyes, using liquid-liquid extraction and UPLC-MS/MS analysis. The calibration curves were linear over the range of 0.1-25 mg/kg. Mean recovery for the eight Sudan dyes ranged from 80.7% to 104.4%, and with the inter-day and intra-day precisions ranged from 2.24% to 12.2%. The method was successfully applied in the determination of Sudan dyes in chili powder of 10 samples.展开更多
Early Permian deposits in north of Kalmard region recognize with formal group of Khan;they have various features in the different place. This group includes four different formations from lower to the upper part: Chil...Early Permian deposits in north of Kalmard region recognize with formal group of Khan;they have various features in the different place. This group includes four different formations from lower to the upper part: Chili, Sartakht and Hermez. These formations consist of carbonate rocks. Chili Formation has 104 m, thickness in Darin section and consists of limestone with intermediates of shale and marland sandstone. Lower boundary of this formation is disconformable with Gachal formation. The upper boundary is separated by unconformity from the upper part Sartakht formation according to the lithological characters and microscopic studies, cause identifications of beach, intertidal, open and semi-restricted lagoon, shoals and bar and open marine sub-environ- ments for the Chili Formation. Vertical changes of microfacies and depth changes curve show much more thickness of shoals and bar microfacies, and little thickness of open and semi-re- stricted lagoon and open marine microfacies. Deposits of Chili Formation in Darin section deposited in the gentle gradient Homoclinal ramp in the south of Paleotethys Ocean. Two depositional sequences have been identified in this formation, based on recognized Fusulinid, show age of Sakmarian, which has adaptation with Lower Absaroka III.展开更多
Chili(Capsicum annuum L.)is the popular spicy vegetable crops belonging to family Solanaceae.Chili peppers are known for their pungency characteristic due to the presence of capsaicinoids that classifies them into hot...Chili(Capsicum annuum L.)is the popular spicy vegetable crops belonging to family Solanaceae.Chili peppers are known for their pungency characteristic due to the presence of capsaicinoids that classifies them into hot or sweet pepper.Chili is used as spices,folk remedies for diseases,vegetables,and coloring agent showing a diverse role in human’s life.However,its production is hampered by different biotic stress and abiotic factors.Similarly,the unavailability of high yielding varieties,high temperature,and disease incidence,particularly,anthracnose disease,are the major constraints responsible for the low production of chili pepper.The advents of molecular markers,advancement in quantitative trait loci by classical genetic analysis,and conventional breeding have shown the number of genes for many important and major traits.While the newly developed genotyping technologies and nextgeneration sequencing have led to the discovery of molecular basis for economic important characters in the chili genome and generate large scale data for genomic resources.Based on this background,this review summarizes progress in the development of anthracnose disease-resistant and heat-tolerant chili genotypes through conventional breeding and molecular approaches.This review would help plant breeders in understanding the phenotypic and genetic make-up of capsicum genotypes and provides opportunities for pyramiding two respected genes with the help of diversified phenotypic and molecular marker evaluation.展开更多
A strain NBT capable of dissolving silicate minerals and promoting plant growth was treated with UV+LiCl. Thirty-two mutants tolerable to 2% NaCl solution were obtained. However, through the survival experiments in hi...A strain NBT capable of dissolving silicate minerals and promoting plant growth was treated with UV+LiCl. Thirty-two mutants tolerable to 2% NaCl solution were obtained. However, through the survival experiments in high osmatic pressure, high temperature and different acidities, two mutants of NBT-6 and NBT-19 were finally obtained. They could survive from 10% NaCl solution and tolerate 55℃, acidic (pH 4) and alkalic (pH 10) conditions. The mutants had the same ability to release K from silicate minerals as the starting strain NBT. Pot experiments with chili and cotton showed that both the mutants developed in the rhi-zosphere soils. The available P and K contents in the rhizosphere soils and plant biomass increased through inoculating these bacteria.展开更多
In this experiment carried out on Caribbean chili pepper plants(Capsicum chinensis),the bio-insecticide azadirachtin in combination with an NPK fertilizer proved to have a greater lethal impact on the larvae of Aedes ...In this experiment carried out on Caribbean chili pepper plants(Capsicum chinensis),the bio-insecticide azadirachtin in combination with an NPK fertilizer proved to have a greater lethal impact on the larvae of Aedes albopictus than each substance on its own.This synergistic effect is noticeably important when both inputs are sprayed directly on the leaves of the plant(foliar application).While the plants treated with azadirachtin or NPK alone cause a 33.6%and 36.4%mortal-ity respectively of the Ae.albopictus larvae,the combination of the two inputs induces a 74.4%mortality on the mosquito larvae.To account for this synergistic effect phenomenon inside the plant,the azadirachtin+NPK combination most likely interacts with the capsaicinoid compounds naturally produced by the plant.Not only does this study carried out on azadirachtin reveal major results but the methodology itself offers a most interesting approach on how to boost the agricultural inputs within the plants.As a matter of fact,this research axis demands developing since the control of pests harmful to men has been dramatically lacking insecticide molecules acting on new targets over the past three decades.展开更多
A total of 398 chili pepper germplasms were used as test materials and genetic diversity and correlation analysis were performed on 17 botanical traits. The results of diversity analysis showed that the diversity inde...A total of 398 chili pepper germplasms were used as test materials and genetic diversity and correlation analysis were performed on 17 botanical traits. The results of diversity analysis showed that the diversity indexes of the 17 botanical traits ranged from 0.15 to 5.97 with the average value of 4.12. The data distributions of 11 quantitative traits were more dispersed than qualitative traits. The average value of eoefficient of variation was 36.90% and the variation ranges were one to six times larger than the average value. The results of correlation analysis showed that plant height had significantly positive correlations with plant breadth, leaf length, leaf width, petiole length, the frrst flower node and carpopodium length. The In'st flower node was significantly negatively correlated with fruit length, fruit width, flesh thickness and weight per fruit and signifieantly positively correlated with plant height and plant breadth. The flesh thickness was significantly positively correlated with leaf length, leaf width, petiole length, fruit length, fruit width and carpopodium length. The weight per fruit was significantly positively correlated with leaf length, leaf width, petiole length, fruit length, fruit width, carpopodium length and flesh thickness. Materials with low first flower node, moderate plant height and width and large fruit should be selected for the breeding of early-maturing and high-yield chili pepper varieties. The genetic distances between chili pepper traits were calculated based on the genotypic values of the 11 quantitative traits. The genetic distances between different traits ranged from 14.26 to 32.99. The 11 quantitative traits were divided into seven groups when the rescaled distance was ten, which further clarified the relationships between different traits. The research results laid a solid foundation for the new variety breeding of chili pepper.展开更多
Cancer is a major cause of morbidity and mortality all over the world and a promising area of cancer research is concentrated on chemoprevention by nutritional compounds. Capsaicin, traditionally used as a food additi...Cancer is a major cause of morbidity and mortality all over the world and a promising area of cancer research is concentrated on chemoprevention by nutritional compounds. Capsaicin, traditionally used as a food additive and an analgesic, is one of the main pungent ingredients in chili peppers. Recent studies have shown that capsaicin has anti-cancer effects in various types of cancer model. The purpose of this review is to outline the anticarcinogenic effect of capsaicin and its mechanism.展开更多
Oligochitosan (OC) with molecular weight Mw of 5000 g/mol was prepared by gamma Co-60 ray irradiation of chitosan solution. Nanosilica (nSiO2) with the size of 10 - 30 nm was synthesized by calcination of acid treated...Oligochitosan (OC) with molecular weight Mw of 5000 g/mol was prepared by gamma Co-60 ray irradiation of chitosan solution. Nanosilica (nSiO2) with the size of 10 - 30 nm was synthesized by calcination of acid treated rice husk. The mixture of 1% OC - 1% nSiO2 was prepared by dispersion of nSiO2 in OC solution. The morphology of nSiO2 in the mixture of OC-nSiO2 was measured from images of transmission electron microscopy (TEM). The effect of foliar application of the mixture of OC-nSiO2 on the induction of resistance against anthracnose disease caused by Colletotrichum gloeosporioides fungus on chili fruits was investigated. Results indicated that foliar application of OC-nSiO2 with the concentration of 60 mg/l - 60 mg/l was found to be as the optimal treatment that reduced the disease severity on chili fruits to 22.2% compared with 90.0% of the control. Thus, OC-nSiO2 hybrid material could be considered as an effective biotic elicitor to prevent anthracnose disease infection for chili fruits. Furthermore, the prepared OC-nSiO2 hybrid material can also be used as an environmentally friendly agrochemical product for sustainable development of agriculture.展开更多
According to the last revised catalogue of the mite family Phytoseiidae and the online Phytoseiidae database,only six species of predatory mites have been identified to date in the Republic of Congo(RC).Two species we...According to the last revised catalogue of the mite family Phytoseiidae and the online Phytoseiidae database,only six species of predatory mites have been identified to date in the Republic of Congo(RC).Two species were reported on cassava(Manihot esculenta),two on coffee(Coffea spp.),one on lemon(Citrus spp.),and one on unidentified plants.In this study,we catalogued predatory mites on five plants of economic interest in the RC.Two hundred and forty-seven mite specimens were collected on Manihot esculenta,Solanum lycopersicum,Solanum melongena,Abelmoschus esculentus,and Capsicum spp.Traditional taxonomy was used to identify the collected specimens.The morphological characteristics of the females were analyzed,including the lengths of the dorsal setae,presence or absence of dorsal and ventrianal setae,shape of insemination apparatus,leg chaetotaxy,and cheliceral dentition.Six species belonging to two sub-families(Phytoseiinae and Amblyseiinae)and five genera(Amblyseius,Euseius,Paraphytoseius,Phytoseius,and Iphiseius)were identified.Among these six species,only one had previously been observed in the RC;the remaining five species are reported.展开更多
Deep Learning has been utilized in computer vision for object detection for almost a decade.Real-time object detection for robotic inspection and harvesting has gained interest during this time as a possible technique...Deep Learning has been utilized in computer vision for object detection for almost a decade.Real-time object detection for robotic inspection and harvesting has gained interest during this time as a possible technique for highqualitymachine assistance during agriculture applications.We utilize RGB and thermal images of chili peppers in an environment of various amounts of debris,pepper overlapping,and ambient lighting,train this dataset,and compare object detection methods.Results are presented from the real-time and less than real-time object detection models.Two advanced deep learning algorithms,Mask-Regional Convolutional Neural Networks(Mask-RCNN)and You Only Look Once version 3(YOLOv3)are compared in terms of object detection accuracy and computational costs.When utilizing the YOLOv3 architecture,an overall training mean average precision(mAP)value of 1.0 is achieved.Most testing images from this model score within a range from 97 to 100%confidence levels in natural environment.It is shown that the YOLOv3 algorithm has superior capabilities to the Mask-RCNNwith over 10 times the computational speed on the chili dataset.However,some of the RGB test images resulted in lowclassification scoreswhen heavy debris is present in the image.A significant improvement in the real-time classification scores was observed when the thermal images were used,especially with heavy debris present.We found and report improved prediction scores with a thermal imagery dataset where YOLOv3 struggled on the RGB images.It was shown that mapping temperature differences between the pepper and plant/debris can provide significant features for object detection in real-time and can help improve accuracy of predictionswith heavy debris,variant ambient lighting,and overlapping of peppers.In addition,successful thermal imaging for real-time robotic harvesting could allow the harvesting period to become more efficient and open up harvesting opportunity in low light situations.展开更多
基金Yichun University Local Development Research Center(Grant No.DF2019002)the PhD Research Foundation of Yichun University(Grant No.211-3360118006)the Animal Care and Ethics Committee of Yichun University(Approval No.2022026).
文摘Background:Benign prostatic hyperplasia(BPH)is a common condition in middle-aged and elderly men.Enlargement of the prostate causes lower urinary tract symptoms.Capsaicin is a phytochemical extracted from chili peppers and exerts many pharmacological actions,such as anti-tumor and anti-inflammatory effects.Methods:Our study investigated the effect of capsaicin in vitro and in a mouse model in vivo.A prostatic stromal myofibroblast cell line(WPMY-1)was co-incubated with testosterone(1µM)and different concentrations of capsaicin(10–100µM)for 24 and 48 h.Capsaicin(10–100µM)significantly inhibited testosterone-treated WPMY-1 cell growth at 48 h by MTT assay.The testosterone propionate(7.5 mg/kg)-induced BPH mouse model was used to examine the anti-proliferative effect of capsaicin.Treatment with capsaicin(10 mg/kg)for 14 days significantly attenuated prostatic hyperplasia.Finasteride was used as a positive control.Results:Capsaicin significantly decreased prostate weight and prostate index(prostate/body weight ratio)in BPH mice.The expression of 5α-reductase type II,androgen receptor(AR)and prostate specific antigen(PSA)protein expression and PSA serum were all significantly reduced in capsaicin-treated BPH mice.In addition,capsaicin also activated transient receptor potential vanilloid 1 mediated apoptosis and autophagy in BPH mice.Conclusion:These results demonstrate multiple positive effects of capsaicin in controlling prostate growth and suggest its therapeutic potential in the treatment of BPH.
基金Supported by General Project of Natural Science Foundation of Hunan Province (2022JJ30312)Enterprise Science and Technology Innovation Team Construction Project of Loudi Science and Technology Innovation Program in 2022.
文摘[Objectives]This study was conducted to investigate the effects of Polygonatum odoratum polysaccharides on the quality of white chili peppers in jars.[Methods]White chili peppers were pickled by adding different concentrations of P.odoratum polysaccharides with traditional pickling technology,and its nitrite content,pH and sensory quality were analyzed and detected.[Results]The results showed that,compared with the control group without adding P.odoratum polysaccharides,the nitrite content in white chili peppers in jars decreased after adding P.odoratum polysaccharides.When the addition amount of P.odoratum polysaccharides was 0.002%,the inhibition rate of nitrite reached the maximum.Under the same fermentation time,P.odoratum polysaccharides could significantly reduce the pH value of pickles.When the addition amount of P.odoratum polysaccharide was 0.002%and the pickling time was 3 months,the sensory quality of white chili peppers in jars was the best.At this point,white chili peppers in jars had rich aroma and tasted soft and sour,and the sour and salinity were suitable.As the fermentation time continued to increase,the chili aroma of white chili peppers in jars gradually decreased,and the sour taste became more pronounced.[Conclusions]This study can provide reference for the safety and quality control of white chili peppers in jars.
文摘Chili sauce shrimp paste (CSSP) is an exotic traditional sauce prepared using mainly fresh chili and shrimp paste well known as sambal terasi (Indonesia) and sambal belacan (Malaysia and Brunei). This study aims to evaluate CSSP from different regions in Indonesia on physiochemical properties, volatile compounds, and sensory evaluation. Evaluation in free amino acid (FAA) and volatile compounds were analyzed using HPLC and GC-MS. CSSP had no significant (p > 0.05) on proximate (except for moisture), salinity, pH, microbial counts and the redness index (a*/b*) compared to control. The identified volatiles can be grouped into hydrocarbon, carbonyl, sulfur, alcohol, nitrogen, fatty acid, and ester compounds. The CSSP A, B, C, D, and E (control) contained 9, 21, 12, 29, and 6 volatile compounds respectively. The total FAA ranged from 44.32 to 67.03 g/100 g, and histidine (his) was found as the most abundant in each CSSP. The rheological properties (yield stress, viscosity, and consistency) of CSSP and control values were significantly, except for CSSP B. The intensities perceived of saltiness, sweetness, and bitterness were greatly varied among CSSP. Although there is no correlation between sweetness and bitterness this is indicated by the negative correlation.
基金sponsored by the National 973 Program of China (2006CB101907)the National 863 Program of China (2006AA10A211, 2008AA10Z414)the National Natural Science Foundation of China(30671048, 30671384)
文摘Azoxystrobin acts as an inhibitor of electron transport by binding to the Qo center of cytochrome b (cyt b). Resistance to azoxystrobin was usually caused by the point mutation of cyt b gene or by the induction of alternative respiration. Oxygen consumption test for mycelia of Colletotrichum capsici showed that azoxystrobin inhibited mycelial respiration within 12 h; however, as time went on, the respiration of the mycelia recovered when the mycelia were treated with azoxystrobin and salicylhydroxamic acid (SHAM, a known inhibitor of alternative respiration), and the oxygen consumption of the mycelia could not be inhibited. Meanwhile, cytochrome b (cyt b) gene expression increased with the recovery of mycelial respiration. The increased cyt b gene expression might play a role in the development of resistance to azoxystrobin in C. capsici.
基金supported by the National Key R&D Program of China(2016YFD0400505)China Postdoctoral Science Foundation(2018M640241)+2 种基金Tianjin Postdoctoral Foundation(TJQYBSH2018010)Tianjin Municipal Education Commission(TD13-5013,2018ZD08)Key Laboratory of Industrial Fermentation Microbiology,Education Ministry of China(2018KF005)。
文摘The entire fermentation process of traditional Chinese broad bean paste with chili comprises three individual stages:Tianbanzi,chili pei,and paste fermentation(Tianbanzi-chili pei mixture).Three stages share average 77.53%of all bacteria(89 genera),indicating that the similar environment leads to the similar bacterial communities.One,one,and three genera are exclusive to Tianbanzi,chili pei,and paste stages,respectively,due to the special physical and chemical properties for each stage.Total acidity,pH,and NaCl are important endogenous factors that promote the succession of bacterial communities.According to the dynamics of organic acids,reducing sugars,amino acids,and volatile compounds,60-,210-,and 180-day are considered the best fermentation periods for Tianbanzi,chili pei,and paste,respectively,to balance time cost and product quality.Three(Tetragenococcus,Lactobacillus,and Pseudomonas),four(Tetragenococcus,Lactobacillus,Bacillus,and Pseudomonas),and five(Tetragenococcus,Lactobacillus,Bacillus,Pseudomonas,and Pediococcus)genera are considered the core functional bacteria of Tianbanzi,chili pei,and paste fermentation,respectively.
文摘Sudan dyes are synthetic azo dyes which are widely used in industry. Although they are not allowed in foodstuffs, they have been found contaminating in different food products and their presence is regularly reported. It is assumed that these appearances are due to cross-contamination or adulteration. In this paper, we present a newly developed fast and sensitive method for the quantification of eight Sudan dyes, using liquid-liquid extraction and UPLC-MS/MS analysis. The calibration curves were linear over the range of 0.1-25 mg/kg. Mean recovery for the eight Sudan dyes ranged from 80.7% to 104.4%, and with the inter-day and intra-day precisions ranged from 2.24% to 12.2%. The method was successfully applied in the determination of Sudan dyes in chili powder of 10 samples.
文摘Early Permian deposits in north of Kalmard region recognize with formal group of Khan;they have various features in the different place. This group includes four different formations from lower to the upper part: Chili, Sartakht and Hermez. These formations consist of carbonate rocks. Chili Formation has 104 m, thickness in Darin section and consists of limestone with intermediates of shale and marland sandstone. Lower boundary of this formation is disconformable with Gachal formation. The upper boundary is separated by unconformity from the upper part Sartakht formation according to the lithological characters and microscopic studies, cause identifications of beach, intertidal, open and semi-restricted lagoon, shoals and bar and open marine sub-environ- ments for the Chili Formation. Vertical changes of microfacies and depth changes curve show much more thickness of shoals and bar microfacies, and little thickness of open and semi-re- stricted lagoon and open marine microfacies. Deposits of Chili Formation in Darin section deposited in the gentle gradient Homoclinal ramp in the south of Paleotethys Ocean. Two depositional sequences have been identified in this formation, based on recognized Fusulinid, show age of Sakmarian, which has adaptation with Lower Absaroka III.
文摘Chili(Capsicum annuum L.)is the popular spicy vegetable crops belonging to family Solanaceae.Chili peppers are known for their pungency characteristic due to the presence of capsaicinoids that classifies them into hot or sweet pepper.Chili is used as spices,folk remedies for diseases,vegetables,and coloring agent showing a diverse role in human’s life.However,its production is hampered by different biotic stress and abiotic factors.Similarly,the unavailability of high yielding varieties,high temperature,and disease incidence,particularly,anthracnose disease,are the major constraints responsible for the low production of chili pepper.The advents of molecular markers,advancement in quantitative trait loci by classical genetic analysis,and conventional breeding have shown the number of genes for many important and major traits.While the newly developed genotyping technologies and nextgeneration sequencing have led to the discovery of molecular basis for economic important characters in the chili genome and generate large scale data for genomic resources.Based on this background,this review summarizes progress in the development of anthracnose disease-resistant and heat-tolerant chili genotypes through conventional breeding and molecular approaches.This review would help plant breeders in understanding the phenotypic and genetic make-up of capsicum genotypes and provides opportunities for pyramiding two respected genes with the help of diversified phenotypic and molecular marker evaluation.
文摘A strain NBT capable of dissolving silicate minerals and promoting plant growth was treated with UV+LiCl. Thirty-two mutants tolerable to 2% NaCl solution were obtained. However, through the survival experiments in high osmatic pressure, high temperature and different acidities, two mutants of NBT-6 and NBT-19 were finally obtained. They could survive from 10% NaCl solution and tolerate 55℃, acidic (pH 4) and alkalic (pH 10) conditions. The mutants had the same ability to release K from silicate minerals as the starting strain NBT. Pot experiments with chili and cotton showed that both the mutants developed in the rhi-zosphere soils. The available P and K contents in the rhizosphere soils and plant biomass increased through inoculating these bacteria.
文摘In this experiment carried out on Caribbean chili pepper plants(Capsicum chinensis),the bio-insecticide azadirachtin in combination with an NPK fertilizer proved to have a greater lethal impact on the larvae of Aedes albopictus than each substance on its own.This synergistic effect is noticeably important when both inputs are sprayed directly on the leaves of the plant(foliar application).While the plants treated with azadirachtin or NPK alone cause a 33.6%and 36.4%mortal-ity respectively of the Ae.albopictus larvae,the combination of the two inputs induces a 74.4%mortality on the mosquito larvae.To account for this synergistic effect phenomenon inside the plant,the azadirachtin+NPK combination most likely interacts with the capsaicinoid compounds naturally produced by the plant.Not only does this study carried out on azadirachtin reveal major results but the methodology itself offers a most interesting approach on how to boost the agricultural inputs within the plants.As a matter of fact,this research axis demands developing since the control of pests harmful to men has been dramatically lacking insecticide molecules acting on new targets over the past three decades.
基金Supported by the Fundamental Scientific Research Funds for Chinese Academy of Tropical Agricultural Sciences(1630032015003)
文摘A total of 398 chili pepper germplasms were used as test materials and genetic diversity and correlation analysis were performed on 17 botanical traits. The results of diversity analysis showed that the diversity indexes of the 17 botanical traits ranged from 0.15 to 5.97 with the average value of 4.12. The data distributions of 11 quantitative traits were more dispersed than qualitative traits. The average value of eoefficient of variation was 36.90% and the variation ranges were one to six times larger than the average value. The results of correlation analysis showed that plant height had significantly positive correlations with plant breadth, leaf length, leaf width, petiole length, the frrst flower node and carpopodium length. The In'st flower node was significantly negatively correlated with fruit length, fruit width, flesh thickness and weight per fruit and signifieantly positively correlated with plant height and plant breadth. The flesh thickness was significantly positively correlated with leaf length, leaf width, petiole length, fruit length, fruit width and carpopodium length. The weight per fruit was significantly positively correlated with leaf length, leaf width, petiole length, fruit length, fruit width, carpopodium length and flesh thickness. Materials with low first flower node, moderate plant height and width and large fruit should be selected for the breeding of early-maturing and high-yield chili pepper varieties. The genetic distances between chili pepper traits were calculated based on the genotypic values of the 11 quantitative traits. The genetic distances between different traits ranged from 14.26 to 32.99. The 11 quantitative traits were divided into seven groups when the rescaled distance was ten, which further clarified the relationships between different traits. The research results laid a solid foundation for the new variety breeding of chili pepper.
文摘Cancer is a major cause of morbidity and mortality all over the world and a promising area of cancer research is concentrated on chemoprevention by nutritional compounds. Capsaicin, traditionally used as a food additive and an analgesic, is one of the main pungent ingredients in chili peppers. Recent studies have shown that capsaicin has anti-cancer effects in various types of cancer model. The purpose of this review is to outline the anticarcinogenic effect of capsaicin and its mechanism.
文摘Oligochitosan (OC) with molecular weight Mw of 5000 g/mol was prepared by gamma Co-60 ray irradiation of chitosan solution. Nanosilica (nSiO2) with the size of 10 - 30 nm was synthesized by calcination of acid treated rice husk. The mixture of 1% OC - 1% nSiO2 was prepared by dispersion of nSiO2 in OC solution. The morphology of nSiO2 in the mixture of OC-nSiO2 was measured from images of transmission electron microscopy (TEM). The effect of foliar application of the mixture of OC-nSiO2 on the induction of resistance against anthracnose disease caused by Colletotrichum gloeosporioides fungus on chili fruits was investigated. Results indicated that foliar application of OC-nSiO2 with the concentration of 60 mg/l - 60 mg/l was found to be as the optimal treatment that reduced the disease severity on chili fruits to 22.2% compared with 90.0% of the control. Thus, OC-nSiO2 hybrid material could be considered as an effective biotic elicitor to prevent anthracnose disease infection for chili fruits. Furthermore, the prepared OC-nSiO2 hybrid material can also be used as an environmentally friendly agrochemical product for sustainable development of agriculture.
文摘According to the last revised catalogue of the mite family Phytoseiidae and the online Phytoseiidae database,only six species of predatory mites have been identified to date in the Republic of Congo(RC).Two species were reported on cassava(Manihot esculenta),two on coffee(Coffea spp.),one on lemon(Citrus spp.),and one on unidentified plants.In this study,we catalogued predatory mites on five plants of economic interest in the RC.Two hundred and forty-seven mite specimens were collected on Manihot esculenta,Solanum lycopersicum,Solanum melongena,Abelmoschus esculentus,and Capsicum spp.Traditional taxonomy was used to identify the collected specimens.The morphological characteristics of the females were analyzed,including the lengths of the dorsal setae,presence or absence of dorsal and ventrianal setae,shape of insemination apparatus,leg chaetotaxy,and cheliceral dentition.Six species belonging to two sub-families(Phytoseiinae and Amblyseiinae)and five genera(Amblyseius,Euseius,Paraphytoseius,Phytoseius,and Iphiseius)were identified.Among these six species,only one had previously been observed in the RC;the remaining five species are reported.
文摘Deep Learning has been utilized in computer vision for object detection for almost a decade.Real-time object detection for robotic inspection and harvesting has gained interest during this time as a possible technique for highqualitymachine assistance during agriculture applications.We utilize RGB and thermal images of chili peppers in an environment of various amounts of debris,pepper overlapping,and ambient lighting,train this dataset,and compare object detection methods.Results are presented from the real-time and less than real-time object detection models.Two advanced deep learning algorithms,Mask-Regional Convolutional Neural Networks(Mask-RCNN)and You Only Look Once version 3(YOLOv3)are compared in terms of object detection accuracy and computational costs.When utilizing the YOLOv3 architecture,an overall training mean average precision(mAP)value of 1.0 is achieved.Most testing images from this model score within a range from 97 to 100%confidence levels in natural environment.It is shown that the YOLOv3 algorithm has superior capabilities to the Mask-RCNNwith over 10 times the computational speed on the chili dataset.However,some of the RGB test images resulted in lowclassification scoreswhen heavy debris is present in the image.A significant improvement in the real-time classification scores was observed when the thermal images were used,especially with heavy debris present.We found and report improved prediction scores with a thermal imagery dataset where YOLOv3 struggled on the RGB images.It was shown that mapping temperature differences between the pepper and plant/debris can provide significant features for object detection in real-time and can help improve accuracy of predictionswith heavy debris,variant ambient lighting,and overlapping of peppers.In addition,successful thermal imaging for real-time robotic harvesting could allow the harvesting period to become more efficient and open up harvesting opportunity in low light situations.