Rhizoma Polygoni Bistortae has been widely used as an important medicinal herb in the Far East for a few thousand years, and it has gained more recognition in the last few decades. In the present study, we used a high...Rhizoma Polygoni Bistortae has been widely used as an important medicinal herb in the Far East for a few thousand years, and it has gained more recognition in the last few decades. In the present study, we used a high-performance capillary electrophoresis （HPCE） method for determination of the gallic acid （GA） in Quanshen （QS） pieces. The analysis of GA in QS pieces was successfully achieved in less than 6 min by capillary electrophoresis with UV detection at a wavelength of 290 nm. The background electrolyte was 20 mmol/L sodium borate solution （pH 9.00）. The separation was performed in an uncoated fused-silica capillary （52 cm^50 lam） with an effective length of 40 cm, and the applied voltage was 20 kV. Sample solutions were injected for l0 s at an atmospheric pressure of 5 KPa. The result showed good resolution and precision （the intra-day RSD was 0.37%）. The calibration curves of the standard solutions were linear in the range of 0.22-2.25 mg/mL, with correlation coefficients greater than 0.9990. The recovery of the method ranged from 100.6% to 107.9%, and the average recovery of GA was 105.9% with an RSD of 3.63%. HPCE is a powerful technology, and it is the first time to successfully apply such a method for the determination of GA in the traditional Chinese medicine.展开更多
Objective:To explore the prescription patterns of different dosage forms of Chinese herbal medicines(CHMs)for the treatment of rheumatoid arthritis(RA)and their effects on immune-inflammatory indices.Methods:Clinical ...Objective:To explore the prescription patterns of different dosage forms of Chinese herbal medicines(CHMs)for the treatment of rheumatoid arthritis(RA)and their effects on immune-inflammatory indices.Methods:Clinical data were collected from patients with RA in 4 hospitals(3 Class A comprehensive hospitals and 1 Class B comprehensive hospital)in Anhui Province,China,from August 2012 to June 2018 via the electronic medical record gathering system.Following extraction of prescription information,each prescribed herb was quantified and standardized according to the knowledge base to establish a database of RA treatment formulae.The medical records were divided into the granules group and decoction pieces group.Core herbs and their combination patterns were obtained from the two groups of cases using Liquorice software.Changes in immune-inflammatory and hepatic and renal function indices were compared between the two groups using SPSS 23.0 software.The Aprior module of SPSS Clementine 11.1 software was applied to analyse the correlation between CHMs and improvement in indices.Finally,the ORACLE 10 g tool was used to evaluate the random walk model of the immune-inflammatory indices between the two groups.Results:(1)We retrospectively analysed 35,898 prescriptions for 6,829 patients with RA who received CHM treatment.There were 3,816 patients in the granules group and 3,013 in the decoction pieces group.(2)The core herbs were Pi(Spleen)-strengthening and dampness-resolving drugs,blood-activating and stasis-resolving drugs,wind/dampness-dispelling drugs and heatclearing and detoxifying drugs.(3)Both dosage forms could improve immune-inflammatory indices in RA patients,with similar efficacy and no influence on hepatic or renal function.(4)Herba Siegesbeckiae and Oldenlandia had a stronger association with immune-inflammatory indices in the two groups.(5)The immune-inflammatory indices showed obvious improvement after treatment with granules and decoction pieces of CHMs,and there were long range correlations between the comprehensive evaluation indices and interventions.Conclusions:The principal CHM treatment methods for RA in four hospitals in Anhui Province are strengthening Pi and resolving dampness,activating blood and resolving stasis,dispelling wind/dampness and clearing heat.Granules and decoction pieces of CHMs have similar efficacy in improving immune-inflammatory indices in RA patients and could be used as treatment options for RA.展开更多
基金supported by Beijing Kaiao Technology Development Co., Ltd., China and Beijing Friendship Hospital, Capital University of Medical Sciences Beijing
文摘Rhizoma Polygoni Bistortae has been widely used as an important medicinal herb in the Far East for a few thousand years, and it has gained more recognition in the last few decades. In the present study, we used a high-performance capillary electrophoresis （HPCE） method for determination of the gallic acid （GA） in Quanshen （QS） pieces. The analysis of GA in QS pieces was successfully achieved in less than 6 min by capillary electrophoresis with UV detection at a wavelength of 290 nm. The background electrolyte was 20 mmol/L sodium borate solution （pH 9.00）. The separation was performed in an uncoated fused-silica capillary （52 cm^50 lam） with an effective length of 40 cm, and the applied voltage was 20 kV. Sample solutions were injected for l0 s at an atmospheric pressure of 5 KPa. The result showed good resolution and precision （the intra-day RSD was 0.37%）. The calibration curves of the standard solutions were linear in the range of 0.22-2.25 mg/mL, with correlation coefficients greater than 0.9990. The recovery of the method ranged from 100.6% to 107.9%, and the average recovery of GA was 105.9% with an RSD of 3.63%. HPCE is a powerful technology, and it is the first time to successfully apply such a method for the determination of GA in the traditional Chinese medicine.
基金Supported by the National Key Research and Development Program of the Ministry of Science and Technology (No. 2018YFC1705204)the Major Science and Technology Project of Anhui Province (No. 17030801009)+3 种基金the Key Research and Development Projects of Foreign Scientific and Technological Cooperation planned in Anhui Province (No. 201904b11020011)the Anhui Provincial Laboratory of Internal Medicine Application and Development of Modern Chinese Medicine (No. 2016080503B041)the Construction Project of LIU Jian Studio of Traditional Chinese Medicine in Anhui Province (Secretary of Development of Traditional Chinese Medicine [2018] No. 11)the 12th Batch of the"115"Innovation Team in Anhui Province (No. 1 [2019] of the Anhui Talent Office)
文摘Objective:To explore the prescription patterns of different dosage forms of Chinese herbal medicines(CHMs)for the treatment of rheumatoid arthritis(RA)and their effects on immune-inflammatory indices.Methods:Clinical data were collected from patients with RA in 4 hospitals(3 Class A comprehensive hospitals and 1 Class B comprehensive hospital)in Anhui Province,China,from August 2012 to June 2018 via the electronic medical record gathering system.Following extraction of prescription information,each prescribed herb was quantified and standardized according to the knowledge base to establish a database of RA treatment formulae.The medical records were divided into the granules group and decoction pieces group.Core herbs and their combination patterns were obtained from the two groups of cases using Liquorice software.Changes in immune-inflammatory and hepatic and renal function indices were compared between the two groups using SPSS 23.0 software.The Aprior module of SPSS Clementine 11.1 software was applied to analyse the correlation between CHMs and improvement in indices.Finally,the ORACLE 10 g tool was used to evaluate the random walk model of the immune-inflammatory indices between the two groups.Results:(1)We retrospectively analysed 35,898 prescriptions for 6,829 patients with RA who received CHM treatment.There were 3,816 patients in the granules group and 3,013 in the decoction pieces group.(2)The core herbs were Pi(Spleen)-strengthening and dampness-resolving drugs,blood-activating and stasis-resolving drugs,wind/dampness-dispelling drugs and heatclearing and detoxifying drugs.(3)Both dosage forms could improve immune-inflammatory indices in RA patients,with similar efficacy and no influence on hepatic or renal function.(4)Herba Siegesbeckiae and Oldenlandia had a stronger association with immune-inflammatory indices in the two groups.(5)The immune-inflammatory indices showed obvious improvement after treatment with granules and decoction pieces of CHMs,and there were long range correlations between the comprehensive evaluation indices and interventions.Conclusions:The principal CHM treatment methods for RA in four hospitals in Anhui Province are strengthening Pi and resolving dampness,activating blood and resolving stasis,dispelling wind/dampness and clearing heat.Granules and decoction pieces of CHMs have similar efficacy in improving immune-inflammatory indices in RA patients and could be used as treatment options for RA.
