[Objective]The paper was to obtain a pathogen-inducible responsive gene TcREMORIN from Taxus chinesis.[Method]Total RNA was extracted by Trizol reagent,and cDNA was synthesized using TaKaRa reverse transcription kit a...[Objective]The paper was to obtain a pathogen-inducible responsive gene TcREMORIN from Taxus chinesis.[Method]Total RNA was extracted by Trizol reagent,and cDNA was synthesized using TaKaRa reverse transcription kit and Invitrogen M-MLV Reverse Transcriptase kit.The TcREMORIN gene was amplified by qRT-PCR.[Result]The full length of TcREMORIN was 778 bp with a 501 bp open reading frame(ORF)which encoded 166 amino acids.Bioinformatics analysis indicated that TcREMORIN possessed a conserved domain of remorin family genes and located probably in cell plasma membrane.The results of real-time quantitative PCR assay revealed that the target gene was induced by anthracnose of T.chinesis and its expression level was up-regulated by PEG,NaCl,H2O2 and 4℃stresses.[Conclusion]The TcREMORIN gene may play a critical role in plant-pathogen interaction.展开更多
分别以库拉索芦荟(Aloe barbadensis Mill.)和中华芦荟(Aloe vera L.var.Chinesis)老叶、中叶、嫩叶的整个叶片及叶片中间凝胶部分为样品,比较2种芦荟不同部位的抗氧化酶活性。结果表明,库拉索芦荟、中华芦荟不同部位抗氧化酶间活性差...分别以库拉索芦荟(Aloe barbadensis Mill.)和中华芦荟(Aloe vera L.var.Chinesis)老叶、中叶、嫩叶的整个叶片及叶片中间凝胶部分为样品,比较2种芦荟不同部位的抗氧化酶活性。结果表明,库拉索芦荟、中华芦荟不同部位抗氧化酶间活性差异较大。库拉索芦荟超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)活性最高部位分别是老叶中间凝胶、中叶整个叶片、老叶整个叶片;中华芦荟SOD、CAT、POD活性最高部位分别是嫩叶中间凝胶、老叶整个叶片、老叶整个叶片。结合生物学产量,开发应用SOD方面,应以2种芦荟中叶中间凝胶为原料;开发应用CAT和POD方面,应以2种芦荟老叶整个叶片为原料。展开更多
Total flavonoids are the main pharmaceutical components of Trollius chinensis Bunge, and orientin and vitexin are the monomer components of total flavonoids in Trollius chinensis Bunge. In this study, an aged mouse mo...Total flavonoids are the main pharmaceutical components of Trollius chinensis Bunge, and orientin and vitexin are the monomer components of total flavonoids in Trollius chinensis Bunge. In this study, an aged mouse model was established through intraperitoneal injection of D-galactose for 8 weeks, followed by treatment with 40, 20, or 10 mg/kg orientin, vitexin, or a positive control （vitamin E） via intragastric administration for an additional 8 weeks. Orientin, vitexin, and vitamin E improved the general medical status of the aging mice and significantly increased their brain weights. They also produced an obvious rise in total antioxidant capacity, superoxide dismutase, catalase, and glutathione peroxidase levels in the serum, and the levels of superoxide dismutase, catalase and glutathione peroxidase, Na＋-K＋-ATP enzyme, and Ca2＋-Mg2＋-ATP enzyme in the liver, brain and kidneys. In addition, they significantly reduced malondialdehyde levels in the liver, brain and kidney and lipofuscin levels in the brain. They also significantly improved the neuronal ultrastructure. The 40 mg/kg dose of orientin and vitexin had the same antioxidant capacity as vitamin E. These experimental findings indicate that orientin and vitexin engender anti-aging effects through their antioxidant capacities.展开更多
The aim of this study was to formulate drug-loaded bio-lipstrips using novel bioexcipients isolated from the fruit pulp of Litchi chinesis(biomaterial L)and to explore the potentiality of lip skin as a novel translabi...The aim of this study was to formulate drug-loaded bio-lipstrips using novel bioexcipients isolated from the fruit pulp of Litchi chinesis(biomaterial L)and to explore the potentiality of lip skin as a novel translabial drug delivery system.The biomaterial,prepared by a simplified economical process and purified by hot dialysis,was subjected to various physicochemical evaluations along with spectral analysis including UV,FT-IR,Mass and ^(1)H NMR.The lipstrip formulated with the novel bioexcipients was screened for its functional properties,including filmability using a film-casting method,and bio/mucoadhesitivity using a shear-stress method,the Park and Robinson method and a rotating cylinder method.Rosiglitazone-loaded bio-lipstrips were formulated by using biomaterial L as a strip former and dextrose as a flexicizer.The formulated strips were subjected to various evaluations,including thickness,folding endurance,in-vitro release and in-vivo release.The release of rosiglitazone maleate was maintained over 24 h,which was confirmed in in-vitro and in-vivo release experiments.Our results reveal that this biopolymer possesses promising stripability as well as bio-adhesitivity.The formulated bio-lipstrips are feasible for delivering rosiglitazone maleate by translabial administration.展开更多
基金Supported by Science and Technology Project of Henan Province(182102110132).
文摘[Objective]The paper was to obtain a pathogen-inducible responsive gene TcREMORIN from Taxus chinesis.[Method]Total RNA was extracted by Trizol reagent,and cDNA was synthesized using TaKaRa reverse transcription kit and Invitrogen M-MLV Reverse Transcriptase kit.The TcREMORIN gene was amplified by qRT-PCR.[Result]The full length of TcREMORIN was 778 bp with a 501 bp open reading frame(ORF)which encoded 166 amino acids.Bioinformatics analysis indicated that TcREMORIN possessed a conserved domain of remorin family genes and located probably in cell plasma membrane.The results of real-time quantitative PCR assay revealed that the target gene was induced by anthracnose of T.chinesis and its expression level was up-regulated by PEG,NaCl,H2O2 and 4℃stresses.[Conclusion]The TcREMORIN gene may play a critical role in plant-pathogen interaction.
文摘分别以库拉索芦荟(Aloe barbadensis Mill.)和中华芦荟(Aloe vera L.var.Chinesis)老叶、中叶、嫩叶的整个叶片及叶片中间凝胶部分为样品,比较2种芦荟不同部位的抗氧化酶活性。结果表明,库拉索芦荟、中华芦荟不同部位抗氧化酶间活性差异较大。库拉索芦荟超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)活性最高部位分别是老叶中间凝胶、中叶整个叶片、老叶整个叶片;中华芦荟SOD、CAT、POD活性最高部位分别是嫩叶中间凝胶、老叶整个叶片、老叶整个叶片。结合生物学产量,开发应用SOD方面,应以2种芦荟中叶中间凝胶为原料;开发应用CAT和POD方面,应以2种芦荟老叶整个叶片为原料。
基金supported by the Foundation of Zhangjiakou Science and Technology Committee, No.0711046D-9 and No.11110015D
文摘Total flavonoids are the main pharmaceutical components of Trollius chinensis Bunge, and orientin and vitexin are the monomer components of total flavonoids in Trollius chinensis Bunge. In this study, an aged mouse model was established through intraperitoneal injection of D-galactose for 8 weeks, followed by treatment with 40, 20, or 10 mg/kg orientin, vitexin, or a positive control （vitamin E） via intragastric administration for an additional 8 weeks. Orientin, vitexin, and vitamin E improved the general medical status of the aging mice and significantly increased their brain weights. They also produced an obvious rise in total antioxidant capacity, superoxide dismutase, catalase, and glutathione peroxidase levels in the serum, and the levels of superoxide dismutase, catalase and glutathione peroxidase, Na＋-K＋-ATP enzyme, and Ca2＋-Mg2＋-ATP enzyme in the liver, brain and kidneys. In addition, they significantly reduced malondialdehyde levels in the liver, brain and kidney and lipofuscin levels in the brain. They also significantly improved the neuronal ultrastructure. The 40 mg/kg dose of orientin and vitexin had the same antioxidant capacity as vitamin E. These experimental findings indicate that orientin and vitexin engender anti-aging effects through their antioxidant capacities.
文摘The aim of this study was to formulate drug-loaded bio-lipstrips using novel bioexcipients isolated from the fruit pulp of Litchi chinesis(biomaterial L)and to explore the potentiality of lip skin as a novel translabial drug delivery system.The biomaterial,prepared by a simplified economical process and purified by hot dialysis,was subjected to various physicochemical evaluations along with spectral analysis including UV,FT-IR,Mass and ^(1)H NMR.The lipstrip formulated with the novel bioexcipients was screened for its functional properties,including filmability using a film-casting method,and bio/mucoadhesitivity using a shear-stress method,the Park and Robinson method and a rotating cylinder method.Rosiglitazone-loaded bio-lipstrips were formulated by using biomaterial L as a strip former and dextrose as a flexicizer.The formulated strips were subjected to various evaluations,including thickness,folding endurance,in-vitro release and in-vivo release.The release of rosiglitazone maleate was maintained over 24 h,which was confirmed in in-vitro and in-vivo release experiments.Our results reveal that this biopolymer possesses promising stripability as well as bio-adhesitivity.The formulated bio-lipstrips are feasible for delivering rosiglitazone maleate by translabial administration.
