ZIF-67/CS对阳离子染料孔雀石绿的吸附性能研究

针对传统吸附剂与水相分离困难的问题,设计并制备了一种环境友好、成本低廉的壳聚糖复合材料ZIF-67/CS。针对吸附剂对孔雀石绿(MG)污染物的吸附性能,考察了污染物浓度、接触时间、吸附剂用量、pH和离子强度对其吸附性能的影响。利用XRD、SEM、FT-IR、TG等对其结构、形貌及热稳定性进行了表征。结果表明,在吸附实验中,303.15 K时ZIF-67/CS对MG的吸附等温线符合Temkin等温吸附模型;吸附动力学拟合结果表明,ZIF-67/CS对MG的吸附行为符合拟二级吸附动力学模型;吸附热力学结果表明吸附过程是自发的、吸热的。ZIF-67/CS对MG的吸附是一个复杂的化学吸附过程,主要由速率决定。

包裹有CTA1-DD蛋白的OCS-DS纳米颗粒的制备及佐剂活性

重组CTA1-DD蛋白具有与完整CT分子相当的全身性和黏膜佐剂功能,但在复杂的生理环境中易被酶或酸降解。本研究以同样具有佐剂活性的O-羧甲基壳聚糖(OCS)和硫酸葡聚糖(DS)为载体,通过离子交联形成纳米颗粒,将CTA1-DD蛋白嵌入其中,使其得到稳定保护。包裹有CTA1-DD蛋白的OCS-DS纳米颗粒的粒径为50~150 nm,Zeta电位约-50 mV,质量浓度1.0 mg/ml的CTA1-DD蛋白投入制备的包裹有CTA1-DD蛋白的OCS-DS纳米颗粒载药率25.33%,包封率86.56%。体外模拟释放试验结果显示CTA1-DD蛋白可在7 d内缓慢释放。将CTA1-DD蛋白与PRV灭活抗原混合后,接种至小鼠鼻腔,结果表明,包裹有CTA1-DD蛋白的OCS-DS纳米颗粒能够同时诱导更高的血清IgG抗体和黏膜IgA抗体表达,证明了其作为黏膜佐剂的高效性。

基于MCS-SBL算法的配电网故障定位方法

配电网拓扑结构复杂,传统方法往往需要大量测点信息且难以实现快速有效的故障定位,本文提出基于少量测点信息的故障定位方法。首先,利用等效原理建立一个欠定的故障节点电压方程;其次,利用多重测量向量模型的贝叶斯压缩感知算法求解方程,根据重构稀疏电流矩阵的非零元素位置求解故障区域,实现故障定位;最后,在IEEE33节点配电系统上进行仿真实验,结果表明,所提方法仅需要少量测点的故障前后正序电压分量便可有效定位故障,计算速度较快,并且基本不受故障类型、过渡电阻的影响,同时适用于单故障和多重故障的场景,具有较强的抗噪能力。

用于碳酸盐岩储层裂缝检测的GWO-CS-BP算法及应用研究

碳酸盐岩储层中的裂隙是油气的运移通道和储集空间,对于油气勘探、开发和评价都具有重要的指导意义。针对研究区碳酸盐岩储层裂缝检测的难题,提出灰狼布谷鸟优化BP算法(GWO-CS-BP),该算法是将GWO-CS(grey wolf-cuckoo search algorithm)与BP(back propagation)相结合形成的裂隙检测方法。将含裂缝信息的相干、曲率、倾角、方位角和构型张量等属性作为GWO-CS-BP神经网络的输入数据,在工区地质资料约束下根据测井数据获得裂缝发育水平评价指标,进而对研究区裂缝发育水平进行评价并划分等级。研究区碳酸盐岩储层裂缝发育水平检测结果表明,GWO-CS-BP算法能够综合各属性特点对研究区的裂缝发育水平特征进行二次误差控制,获得裂缝发育水平评价指标f s并将研究区裂缝发育水平划分为3个等级及4个裂缝存在区域。其中,当研究区裂缝发育水平参数的值适中时,即f s的值大于4.0且小于5.8时,C区域最有利于油气的聚集,高产井的分布数量较多。利用GWO-CS-BP算法对研究区的裂缝发育水平进行了精细评价,并得出裂隙发育水平参数f s,实现了GWO-CS算法改进的BP神经网络在裂缝检测中的有效应用。

BC/CS复合水凝胶的3D打印工艺研究

以细菌纤维素(BC)和壳聚糖(CS)为基质的水凝胶同时具有细菌纤维素的高生物相容性和壳聚糖的抗菌性能,在伤口敷料、组织工程等领域获得了广泛的应用,但其3D打印仍面临困难。本文利用BC和CS的物理特性和其交联机制,制备出可用于3D打印的BC/CS复合水凝胶油墨,同时使用纳米粘土作为流变改性剂,提升水凝胶的自支撑能力。对于交联方法,不再采用传统的浸泡方式,使用喷雾器将戊二醛溶液均匀喷洒在打印样品表面,既不会破坏样品的结构,也可以达到交联的目的。接着,构建了一种适合BC/CS复合水凝胶打印的3D打印系统,并在此基础上研究分析了喷头挤出压力、移动速度、打印高度对水凝胶精度的影响。本文提出的方法有望实现BC/CS复合水凝胶的墨水直写(DIW)打印提供指导。

Cs/O沉积Na_(2)KSb光电阴极表面的第一性原理研究

Na_(2)KSb光电阴极在光电倍增管、图像增强器、真空电子源等领域具有重要应用.为指导高灵敏度Na_(2)KSb光电阴极的制备,采用第一性原理计算方法,研究不同表面取向和原子终止面的Na_(2)KSb表面模型,获得稳定且最有利于电子发射的表面结构.基于该表面进一步研究了不同覆盖度下的Cs原子沉积和Cs/O原子共沉积对Na_(2)KSb表面电子结构和光学性质的影响.对比表面能、吸附能和吸附前后的功函数结果表明,Na_(2)KSb(111)K表面具有优越的电子发射能力以及良好的稳定性.当Na_(2)KSb(111)K表面吸附2/4单层的Cs原子和1/4单层O原子时,获得最大功函数下降量0.16 eV.表面吸附Cs/O原子有利于电荷往表面上方转移,并产生电荷累积,能形成有效表面偶极矩.通过分析能带结构和态密度,发现吸附Cs原子对导带底存在额外的能带贡献,且引入O原子吸附后价带发生上移.此外,吸附Cs/O原子有利于增强表面近红外光吸收,但是会导致表面紫外和可见光吸收变差.

Activation of endogenous neurogenesis and angiogenesis by basic fibroblast growth factor-chitosan gel in an adult rat model of ischemic stroke

Attempts have been made to use cell transplantation and biomaterials to promote cell proliferation,differentiation,migration,and survival,as well as angiogenesis,in the context of brain injury.However,whether bioactive materials can repair the damage caused by ischemic stroke by activating endogenous neurogenesis and angiogenesis is still unknown.In this study,we applied chitosan gel loaded with basic fibroblast growth factor to the stroke cavity 7 days after ischemic stroke in rats.The gel slowly released basic fibroblast growth factor,which improved the local microenvironment,activated endogenous neural stem/progenitor cells,and recruited these cells to migrate toward the penumbra and stroke cavity and subsequently differentiate into neurons,while enhancing angiogenesis in the penumbra and stroke cavity and ultimately leading to partial functional recovery.This study revealed the mechanism by which bioactive materials repair ischemic strokes,thus providing a new strategy for the clinical application of bioactive materials in the treatment of ischemic stroke.

Mo掺杂对笼目超导体CsV_(3)Sb_(5)结构和电磁性能的影响

新型笼目金属CsV_(3)Sb_(5)(A=K, Rb, Cs)作为一个具有多种电子序耦合与竞争的平台,其丰富的物理特性备受关注。本文用新开发的以CsCl为辅助熔剂制备了不同掺Mo含量的Cs(V_(1-x)Mo_(x))3Sb5多晶样品,并通过对XRD数据进行Rietveld分析获得了其晶格结构信息,通过测量各样品的磁化强度及电阻率随温度变化关系,对其磁性和电输运性质进行了表征和分析。结果表明,随着Mo的掺入,母相CsV_(3)Sb_(5)的晶格常数a和晶胞体积略微增大,锑烯层中的Sb2原子会逐渐偏离笼目层。同时,体系的超导电性被抑制,而电荷密度波转变温度却增大,二者呈相互竞争关系。

两种高氮化合物对CS混合燃烧剂性能的影响

为提高燃烧型CS催泪弹的性能,通过在CS混合燃烧剂中添加不同含量的新型高氮含能材料5,5’-偶氮四唑铵盐(AZT)和5,5’-联四唑铵盐(BTA)制备了改性混合燃烧剂。采用气相色谱法测定改性混合燃烧剂的CS气化率,通过DSC测试改性混合燃烧剂的相容性,并测试其撞击感度、摩擦感度。结果表明:一定添加量的AZT和BTA能够提高CS气化率;AZT与CS混合燃烧剂相容,BTA与CS混合燃烧剂不相容;在撞击感度和摩擦感度测试条件下AZT改性混合燃烧剂均不发火。研究表明AZT可用作CS混合燃烧剂的添加剂组分。

Synthesis of chitosan oligosaccharide selenium and its antitumor activity

Background:Polysaccharides have various biological activities;the complexation of polysaccharides with trace element ions can produce synergistic effects,improving the original biological activities of sugars and trace elements.Methods:The preparation process of chitosan oligosaccharide selenium(COSSe)was optimized by the response surface method,followed by a detailed analysis of the resultant compound’s characteristics.The anti-cancer activity of COSSe was studied using the human ovarian cancer cell line SKOV3 as a cell model.Results:The prepared COSSe response surface was well predicted,indicating successful chitosan oligosaccharide binding with selenium.Response surface method analyses identified the optimal synthesis conditions for COSSe:the reaction time of 5.08 h,the reaction temperature of 71.8°C,and mass ratio(Na2SeO3:chitosan oligosaccharide)of 1.02.Under the optimal conditions,the final product,the selenium content,reached 1.302%.The results of cell experiments showed that COSSe significantly inhibited SKOV3 proliferation in a concentration-dependent manner.RNA-seq results showed that chitosan oligosaccharide and COSSe significantly modulated the expression of genes’DNA metabolic processes and cell cycle in SKOV3 cells.Gene enrichment analysis showed the inhibition of the cell cycle,and the results of flow cytometry showed that SKOV3 cells increased in the S phase and decreased in the G2/M phase,with a noted suppression in the protein expression of cyclin-dependent kinase 2(CDK2)and cyclin A1(CCNA1).Conclusion:COSSe has a stronger effect than chitosan oligosaccharide,leading to the arrest of the cell cycle in the S phase.Thus,COSSe may be an effective candidate for the treatment of ovarian cancer.

多模态视域下高职课程思政4Cs融合式教学研究——以外贸专业课程为例

高职外贸专业既有语言类专业的人文性、思想性特点、又兼具商贸类专业的社会性、实践性特点。从教学模式的中观层面和教学设计的微观层面展开研究,以课程思政为引领,基于高职教育“以就业为导向”的类型属性,结合外贸类专业的学科特点,以4Cs为理论依据,以多模态教学内容为载体构建高职外贸专业融合式教学模式,并探讨多模态教学对学生知识技能发展和思政育人成果的三重影响,对高职课程思政建设有积极促进作用。

Constructing a biofunctionalized 3D-printed gelatin/sodium alginate/chitosan tri-polymer complex scaffold with improvised biological andmechanical properties for bone-tissue engineering

Sodium alginate(SA)/chitosan(CH)polyelectrolyte scaffold is a suitable substrate for tissue-engineering application.The present study deals with further improvement in the tensile strength and biological properties of this type of scaffold to make it a potential template for bone-tissue regeneration.We experimented with adding 0%–15%(volume fraction)gelatin(GE),a protein-based biopolymer known to promote cell adhesion,proliferation,and differentiation.The resulting tri-polymer complex was used as bioink to fabricate SA/CH/GEmatrices by three-dimensional(3D)printing.Morphological studies using scanning electron microscopy revealed the microfibrous porous architecture of all the structures,which had a pore size range of 383–419μm.X-ray diffraction and Fourier-transform infrared spectroscopy analyses revealed the amorphous nature of the scaffold and the strong electrostatic interactions among the functional groups of the polymers,thereby forming polyelectrolyte complexes which were found to improve mechanical properties and structural stability.The scaffolds exhibited a desirable degradation rate,controlled swelling,and hydrophilic characteristics which are favorable for bone-tissue engineering.The tensile strength improved from(386±15)to(693±15)kPa due to the increased stiffness of SA/CH scaffolds upon addition of gelatin.The enhanced protein adsorption and in vitro bioactivity(forming an apatite layer)confirmed the ability of the SA/CH/GE scaffold to offer higher cellular adhesion and a bone-like environment to cells during the process of tissue regeneration.In vitro biological evaluation including the MTT assay,confocal microscopy analysis,and alizarin red S assay showed a significant increase in cell attachment,cell viability,and cell proliferation,which further improved biomineralization over the scaffold surface.In addition,SA/CH containing 15%gelatin designated as SA/CH/GE15 showed superior performance to the other fabricated 3D structures,demonstrating its potential for use in bone-tissue engineering.

Chitosan/Sodium Alginate Multilayer pH-Sensitive Films Based on Layer-by-Layer Self-Assembly for Intelligent Packaging

The abuse of plastic food packaging has brought about severe white pollution issues around the world.Developing green and sustainable biomass packaging is an effective way to solve this problem.Hence,a chitosan/sodium alginate-based multilayer film is fabricated via a layer-by-layer(LBL)self-assembly method.With the help of superior interaction between the layers,the multilayer film possesses excellent mechanical properties(with a tensile strength of 50 MPa).Besides,the film displays outstanding water retention property(blocking moisture of 97.56%)and ultraviolet blocking property.Anthocyanin is introduced into the film to detect the food quality since it is one natural plant polyphenol that is sensitive to the pH changes ranging from 1 to 13 in food when spoilage occurs.It is noted that the film is also bacteriostatic which is desired for food packaging.This study describes a simple technique for the development of advanced multifunctional and fully biodegradable food packaging film and it is a sustainable alternative to plastic packaging.

基于IMOCS-BP神经网络的锂离子电池SOH估计

锂离子电池随着循环充放电次数的增长,其健康状态SOH(state-of-health)会随之发生一定程度的衰减。针对以上问题,设计了一种基于改进的多目标布谷鸟搜索IMOCS(improved multi-objective Cuckoo search)-BP神经网络的锂离子电池健康状态估计方法,在避免算法陷入局部最优的同时自适应改变布谷鸟搜索CS(Cuckoo search)算法更新概率和搜索步长,解决CS算法收敛速度慢和求解精度低的问题。以IMOCS算法和BP神经网络结合,对节点空间范围进行全局搜索,降低权值和阈值的初值对BP神经网络的影响,实现参数优化。通过Matlab仿真,验证了基于IMOCS-BP神经网络的SOH估计算法误差低、性能强,实现了锂电池SOH的精准预测。

Study of Hemorrhoid Disease in the Department of General Surgery of the Cs Ref of the Commune I Bamako

This work was a retrospective prospective study carried out in the general surgery department of the Cs ref of commune I from January 2009 to December 2012. Our study aimed to determine the hospital frequency of hemorrhoidal disease, to identify the contributing factors, describe the clinical and therapeutic aspects and analyze the surgical consequences. We collected 73 patients with an average age of 37.8 years (range: 19 years and 77 years) with a sex ratio of 2.5 in favor of men. We operated on 49 patients or 67.1% of cases. 71.2% of our patients were married and lived in Bamako. Schoolchildren and middle managers made up 39.8% of cases. 82.2% of patients were seen in ordinary consultation and 69.9% complained of progressive anal pain in 66.7% of cases. This pain was triggered by defecation in 88.2% of our patients. The contributing factors were dominated by constipation (53.4%), working in a seated position (41.1%) and a sedentary lifestyle (16.4%) of cases. Stage IV external hemorrhoids and hemorrhoidal thrombosis represented 53.8% of surgical indications. The most used surgical technique was that of Milligan-Morgan, i.e. 51.9% of cases and 45.2% of patients treated medically received a combination of transit regulator, venotonics and analgesics. The surgical aftermath was marked by hemorrhage (3 cases), delayed healing (5 cases), urinary retention (1 case) and scarring anal stenosis (1 case). The morbidity rate was 5.48% of cases and a zero mortality rate. The average length of hospitalization was 2 days with extremes of 1 and 5 days.

Chitosan-based thermosensitive hydrogel with long-term release of murine nerve growth factor for neurotrophic keratopathy

Neurotrophic keratopathy is a persistent defect of the corneal epithelium,with or without stromal ulceration,due to corneal nerve deficiency caused by a variety of etiologies.The treatment options for neurotrophic keratopathy are limited.In this study,an ophthalmic solution was constructed from a chitosan-based thermosensitive hydrogel with long-term release of murine nerve growth factor(CTH-mNGF).Its effectiveness was evaluated in corneal denervation(CD)mice and patients with neurotrophic keratopathy.In the preclinical setting,CTH-mNGF was assessed in a murine corneal denervation model.CTH-mNGF was transparent,thermosensitive,and ensured sustained release of mNGF for over 20 hours on the ocular surface,maintaining the local mNGF concentration around 1300 pg/mL in vivo.Corneal denervation mice treated with CTH-mNGF for 10 days showed a significant increase in corneal nerve area and total corneal nerve length compared with non-treated and CTH treated mice.A subsequent clinical trial of CTH-mNGF was conducted in patients with stage 2 or 3 neurotrophic keratopathy.Patients received topical CTH-mNGF twice daily for 8 weeks.Fluorescein sodium images,Schirmer’s test,intraocular pressure,Cochet-Bonnet corneal perception test,and best corrected visual acuity were evaluated.In total,six patients(total of seven eyes)diagnosed with neurotrophic keratopathy were enrolled.After 8 weeks of CTH-mNGF treatment,all participants showed a decreased area of corneal epithelial defect,as stained by fluorescence.Overall,six out of seven eyes had fluorescence staining scores<5.Moreover,best corrected visual acuity,intraocular pressure,Schirmer’s test and Cochet-Bonnet corneal perception test results showed no significant improvement.An increase in corneal nerve density was observed by in vivo confocal microscopy after 8 weeks of CTH-mNGF treatment in three out of seven eyes.This study demonstrates that CTH-mNGF is transparent,thermosensitive,and has sustained-release properties.Its effectiveness in healing corneal epithelial defects in all eyes with neurotrophic keratopathy suggests CTH-mNGF has promising application prospects in the treatment of neurotrophic keratopathy,being convenient and cost effective.

Simple preparation of C(CS)/g-C_(3)N_(4)/Co carbon aerogel and its catalytic performance for ammonium perchlorate

Biomass chitosan(CS)was used as a template,graphitic phase carbon nitride(g-C_(3)N_(4))with high nitrogen content and certain catalytic activity was used as a dopant,and nano-transition metal cobalt(Co)was used as a catalytic center point.The carbon aerogel(C(CS)/g-C_(3)N_(4)/Co)with a three-dimensional network-like structure was prepared by assembling the three materials through experimental operations such as freeze-drying and high-temperature carbonization.It was demonstrated by scanning and transmission characterization that the CS in the carbon aerogel could provide more active sites for the cobalt nanoparticles,and the doping of graphite-phase carbon nitride as a template dispersed the cobalt nanoparticles and changed the conductivity of the CS.To investigate the catalytic effect of carbon aerogel on ammonium perchlorate(AP),it was investigated by differential thermal analyzer and TG thermal analysis.This carbon aerogel was very effective in catalyzing AP,and the 10 wt% content of the catalyst reduced the AP pyrolysis peak from 703.9 to 595.5 K.And to further investigate the synergistic effect of the three materials,further carbon aerogels such as C(CS)/Co,g-C_(3)N_(4)/Co were prepared and applied to catalyze AP,and the same ratio reduced the AP pyrolysis peak by 98.1℃ and 97.7℃.This result indicates a synergistic effect of the assembly of the three materials.

Smart Colorimetric Corn Starch Films Combined with Anthocyanin-Loaded Glutenin-Carboxymethyl Chitosan Nanocomplexes for Freshness Monitoring of Chilled Pork

In this study,intelligent,pH-responsive colorimetric films were prepared by encapsulating anthocyanins in nanocomplexes prepared from glutenin and carboxymethyl chitosan.These nanocomplexes were added to a corn starch matrix and used in the freshness monitoring of chilled pork.The effects of anthocyanin-loaded nanocomplexes on the physical,structural,and functional characteristics of the films were investigated.The addition of anthocyanin-loaded nanocomplexes increased the tensile strength,elongation at break,hydrophobicity,and light transmittance of the films while decreasing their water vapor permeability.This is because new hydrogen bonds are formed between the film components,resulting in a more homogeneous and dense structure.The colorimetric film has a significant color response to pH changes.These films were used in experiments on the freshness of chilled pork,in which the pH changes with changing freshness states.The results show that the colorimetric film can monitor changes in the freshness of chilled pork in real time,where orange,pink,and green represent the fresh,secondary fresh,and putrefied states of pork,respectively.Therefore,the intelligent colorimetric film developed in this study has good application potential in the food industry.

Perception and Management of Hemorroid Disease at the CSREF of the Commune Bamako Mali

This work was a prospective transversal study over 6 (six) months from May 2 to December 31, 2017 at the Reference Health Center of Commune I and the Chérif la Confessional Health Center in Bamako. The aim was to study the knowledge, attitudes and behavioral practices linked to hemorrhoids in Commune I of the Bamako district, to evaluate the frequency of hemorrhoidal disease, to determine the knowledge, attitudes and behavioral practices linked to hemorrhoids among patients, their companions and traditional therapists in Commune I of the district of Bamako and the factors which influence the motivations and decisions to resort to medical-surgical care and traditional medicine in patients suffering from hemorrhoids in order to analyze the results of the management of hemorrhoidal disease at Cs Ref CI. We collected 36 patients with hemorrhoidal disease, 25 caregivers and 15 traditional hemorrhoidal disease therapists. The sex ratio was 1.25 for patients;6.5 for traditional therapists and 1.5 for accompanying people. The average age of the patients was 32.75 years;49 years for traditional therapists and 28.76 years for those accompanying them. 55.6% of patients claim to know about hemorrhoidal disease;100% of traditional therapists and 80% of accompanying people. The dietary factor of hemorrhoidal disease was mentioned by 90% of patients;66.7% of traditional therapists and 100% of caregivers. Most patients initially resorted to traditional treatment out of fear of surgery and its after-effects. The patients were treated medically and surgically.

Postoperative Complications in the General Surgery Department of the Cs Ref of Commune I of the District of Bamako Mali

Postoperative complications represent important indicators for the quality of surgical care. The objectives of this work were to study post-operative complications in the general surgery department of the Cs ref of commune I in Mali, to determine the frequency of post-operative complications, to identify the factors of occurrence, to describe the clinical aspects, diagnostic and therapeutic in order to assess the additional cost linked to post-operative complications. This study was prospective, carried out in the general surgery department on 300 cases of surgical interventions. It covered all patients aged at least 15 years, operated on and hospitalized or not, and who presented complications during the 30 days postoperatively. Patients under the age of 15 were not included (our general surgery department is not a pediatric surgery department). We collected 300 patients among whom 199 (66.33%) were men and 101 (33.66%) women, i.e. a sex ratio = 1.9. The average age was 38 years with extremes of 15 and 87 years. The main initial diagnoses were: acute appendicitis, peritonitis, occlusions, wall hernias, hemorrhoids, uterine prolapse, uterine myomas, ovarian cysts and acute cholecystitis. Emergencies represented 43% (N = 129) of interventions with 6.98% post-operative complications (POC). Postoperative complications were dominated by surgical site infections, 75% of cases (N = 20), wall hemorrhage 5% (N = 1), testicular necrosis 5% (N = 1) and testicular calcification 5% (N = 1), wire rejection 5% (N = 1) and death 5% (N = 1). The management of postoperative complications was surgical in 95% and medical in 100%. Their occurrence extended the hospital stay by 3.65 days and increased the average cost of care by 60541.85 CFA francs. The mortality index lowered by efficient management of complications (IMAGE) calculated in relation to deaths was 95% of cases. Anemia, ASA score ≥ III, Alteimeir II and IV classes, duration of surgery and post-operative hospitalization were factors contributing to post-operative complications.