In order to evaluate the cytocompatibility and biocompatibility of a new kind of chitosan blend film asa carrier of corneal endothelial cell,rabbit corneal endothelial cells cultured in vitro were breeded ontothe film...In order to evaluate the cytocompatibility and biocompatibility of a new kind of chitosan blend film asa carrier of corneal endothelial cell,rabbit corneal endothelial cells cultured in vitro were breeded ontothe film.After a cell monolayer formed,the scanning electron micrography was performed.After inplant-ed into anterior chamber,slit lamp observation,thickness metering,specular microscopy and HE stainingwere performed at random time after operation to evaluate the bioeompatibility.Inflammation in anterior,thickness of comea,cell density,hexagonality and cell size of the surgical cornea were taken as the in-dexes of biocompatibility.The cultured cells exhibited a confluent monolayer 10 days after incubation,which proved the satisfactory eytoeompatibility of this film.Bioeompatibility assay results suggested theimplantation feasibility of the film as a carrier of corneal endothelial cells.展开更多
To investigate the enhancement of immunological activity of CpG ODN by chitosan gene carrier in mice, the effect of lymphocyte proliferation was detected in mice by using MTT, the levels of IgG and cytokines （IL-2 an...To investigate the enhancement of immunological activity of CpG ODN by chitosan gene carrier in mice, the effect of lymphocyte proliferation was detected in mice by using MTT, the levels of IgG and cytokines （IL-2 and IL-12） in serum were measured by ELISA and peripheral blood T lymphocyte subsets CD4^＋, CD8^＋ were analyzed by flow cytometry. Our results showed that spleen lymphocytes isolated from the CS-CpG ODN group of mice showed the strongest proliferation （SI =1.551）, and the levels of IgG, IL-2 and IL-12 in serum were higher than those of other groups. Compared with the immunization with CpG ODN, the immunization with CS-CpG ODN gene carrier was more efficient in up-regulating the percentage of CD4^＋T cells and the ratio of CD4^＋/CD8^＋ of mice, It was concluded that CS gene carrier of CpG ODN was much more effective in improving immunity of CpG ODN in mice.展开更多
基金the High Technology Research and Development Program of China(2003AA625050)the National Natural Science Foundation of China(30070220)
文摘In order to evaluate the cytocompatibility and biocompatibility of a new kind of chitosan blend film asa carrier of corneal endothelial cell,rabbit corneal endothelial cells cultured in vitro were breeded ontothe film.After a cell monolayer formed,the scanning electron micrography was performed.After inplant-ed into anterior chamber,slit lamp observation,thickness metering,specular microscopy and HE stainingwere performed at random time after operation to evaluate the bioeompatibility.Inflammation in anterior,thickness of comea,cell density,hexagonality and cell size of the surgical cornea were taken as the in-dexes of biocompatibility.The cultured cells exhibited a confluent monolayer 10 days after incubation,which proved the satisfactory eytoeompatibility of this film.Bioeompatibility assay results suggested theimplantation feasibility of the film as a carrier of corneal endothelial cells.
基金This work was supported by a grant from Natural Science Foundation of Hubei province (No.2006ABA134)National Natural Sciences Foundation (No.30170051)
文摘To investigate the enhancement of immunological activity of CpG ODN by chitosan gene carrier in mice, the effect of lymphocyte proliferation was detected in mice by using MTT, the levels of IgG and cytokines （IL-2 and IL-12） in serum were measured by ELISA and peripheral blood T lymphocyte subsets CD4^＋, CD8^＋ were analyzed by flow cytometry. Our results showed that spleen lymphocytes isolated from the CS-CpG ODN group of mice showed the strongest proliferation （SI =1.551）, and the levels of IgG, IL-2 and IL-12 in serum were higher than those of other groups. Compared with the immunization with CpG ODN, the immunization with CS-CpG ODN gene carrier was more efficient in up-regulating the percentage of CD4^＋T cells and the ratio of CD4^＋/CD8^＋ of mice, It was concluded that CS gene carrier of CpG ODN was much more effective in improving immunity of CpG ODN in mice.
