The purpose of this research is to investigate the effects of the variously sulfated chitosans on lysozyme activity and structure. It was shown that the specific enzymatic activity of lysozyme remained almost similar ...The purpose of this research is to investigate the effects of the variously sulfated chitosans on lysozyme activity and structure. It was shown that the specific enzymatic activity of lysozyme remained almost similar to the native protein after being bound to 6-O-sulfated chitosan （6S-chitosan） and 3,6-O-sulfated chitosan （3,6S-chitosan）, but decreased greatly after being bound to 2-N-6-O-sulfated chitosan （2,6S-chitosan）. Meanwhile, among these sulfated chitosans, 2,6S-chitosan induced the greatest conformational change in lysozyme as indicated by the fluorescence spectra. These findings demonstrated that when sulfated chitosans of different structures bind to lysozyme, lysozyme undergoes conformational change of different magnitudes, which results in corresponding levels of lysozyme activity. Further study on the interaction of sulfated chitosans with lysozyme by surface plasmon resonance （SPR） suggested that their affinities might be determined by their molecular structures.展开更多
基金supported by the National Natural Science Foundation of China(Nos.20920102035,20974086, and 21074083)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)
文摘The purpose of this research is to investigate the effects of the variously sulfated chitosans on lysozyme activity and structure. It was shown that the specific enzymatic activity of lysozyme remained almost similar to the native protein after being bound to 6-O-sulfated chitosan （6S-chitosan） and 3,6-O-sulfated chitosan （3,6S-chitosan）, but decreased greatly after being bound to 2-N-6-O-sulfated chitosan （2,6S-chitosan）. Meanwhile, among these sulfated chitosans, 2,6S-chitosan induced the greatest conformational change in lysozyme as indicated by the fluorescence spectra. These findings demonstrated that when sulfated chitosans of different structures bind to lysozyme, lysozyme undergoes conformational change of different magnitudes, which results in corresponding levels of lysozyme activity. Further study on the interaction of sulfated chitosans with lysozyme by surface plasmon resonance （SPR） suggested that their affinities might be determined by their molecular structures.
