期刊文献+
共找到2,361篇文章
< 1 2 119 >
每页显示 20 50 100
Epicatechin attenuates lead(Pb)-induced cognitive impairment in mice:regulation on Nrf2 signaling pathway,and interference on the interaction between Pb with albumin
1
作者 Dai Cheng Qianqian Yu +2 位作者 Kexin Zhu Dingdong Bu Zijian Wu 《Food Science and Human Wellness》 SCIE CSCD 2024年第2期1065-1078,共14页
Epicatechin(EC)was used in this study to antagonize the cognitive dysfunction caused by lead(Pb)exposure in mice.Eight-week-old male Kunming mice were treated with PbCl_(2)(20 mg/kg)and/or EC(50 mg/kg)by gavage admini... Epicatechin(EC)was used in this study to antagonize the cognitive dysfunction caused by lead(Pb)exposure in mice.Eight-week-old male Kunming mice were treated with PbCl_(2)(20 mg/kg)and/or EC(50 mg/kg)by gavage administration for 4 weeks.Morris water maze test showed that EC could improve memory dysfunction induced by Pb.EC antagonized Ca^(2+)overload,activated Nrf2 signaling pathway and reduced the accumulation of Pb in the brain and serum,which suggested that EC might alter Pb distribution in mice.In vitro,spectroscopic analysis,potentiometric titration and docking studies were applied to inquiry into the interaction between bovine serum albumin(BSA)and Pb^(2+)in presence or absence of EC.EC was proved to chelate Pb^(2+)and reduced the interaction between BSA and Pb^(2+).In summary,EC might protect Pb-induced cognitive impairment by activating Nrf2 signaling pathway,and suppressing Pb accumulation via interference on the binding of Pb to albumin. 展开更多
关键词 LEAD EPICATECHIN Bovine serum albumin Interaction Oxidative stress
下载PDF
Interaction Between Gatifloxacin and Bovine Serum Albumin 被引量:7
2
作者 严拯宇 邵秀芬 +1 位作者 严琳 胡育筑 《Journal of Chinese Pharmaceutical Sciences》 CAS 2005年第1期33-37,共5页
Aim To study the reaction mechanism between gatifloxacin and bovine serumalbumin (BSA) at different pHs. Methods Fluorescence spectra and UV absorbance spectra were used.Results The binding constants were determined f... Aim To study the reaction mechanism between gatifloxacin and bovine serumalbumin (BSA) at different pHs. Methods Fluorescence spectra and UV absorbance spectra were used.Results The binding constants were determined from a double reciprocal Lineweaver-Burk curves atdifferent pHs. The binding distance r under normal physiological condition was obtained according toFoster theory of non-radiative energy transfer. The binding force between gatifloxacin and BSA wasinferred by thermody-namical coordination. Conclusion The interaction between gatifloxacin and BSAseems to be strong and the main binding force is electrostatic force. 展开更多
关键词 fluorescence quenching bovine serum albumin GATIFLOXACIN
下载PDF
Multi-spectroscopic characterization of bovine serum albumin upon interaction with atomoxetine 被引量:11
3
作者 Arunkumar T. Buddanavar Sharanappa T. Nandibewoor 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2017年第3期148-155,共8页
The quenching interaction of atomoxetine(ATX) with bovine serum albumin(BSA) was studied in vitro under optimal physiological condition(pH=7.4) by multi-spectroscopic techniques. The mechanism of ATX-BSA system was a ... The quenching interaction of atomoxetine(ATX) with bovine serum albumin(BSA) was studied in vitro under optimal physiological condition(pH=7.4) by multi-spectroscopic techniques. The mechanism of ATX-BSA system was a dynamic quenching process and was confirmed by the fluorescence spectra and lifetime measurements. The number of binding sites, binding constants and other binding characteristics were computed. Thermodynamic parameters ΔH^0 and ΔS^0 indicated that intermolecular hydrophobic forces predominantly stabilized the drug-protein system. The average binding distance between BSA and ATX was studied by F?rsters theory. UV-absorption, Fourier transform infrared spectroscopy(FT-IR), circular dichroism(CD), synchronous spectra and three-dimensional(3D) fluorescence spectral results revealed the changes in micro-environment of secondary structure of protein upon the interaction with ATX. Displacement of site probes and the effects of some common metal ions on the binding of ATX with BSA interaction were also studied. 展开更多
关键词 ATOMOXETINE BOVINE serum albumin 3D fluorescence spectra FT-IR Energy transfer Lifetime measurement
下载PDF
Spectroscopic analysis on the binding interaction of biologically active pyrimidine derivative with bovine serum albumin 被引量:8
4
作者 Vishwas D. Suryawansht Laxman S. Walekar +2 位作者 Anil H. Gore Prashant V. Anbhule Govind B. Kolekar 《Journal of Pharmaceutical Analysis》 SCIE CAS 2016年第1期56-63,共8页
A biologically active antibacterial reagent, 2-amino-6-hydroxy-4-(4-N, N-dimethylaminophenyl)-pyr- imidine-5-carbonitrile (AHDMAPPC), was synthesized. It was employed to investigate the binding in- teraction with ... A biologically active antibacterial reagent, 2-amino-6-hydroxy-4-(4-N, N-dimethylaminophenyl)-pyr- imidine-5-carbonitrile (AHDMAPPC), was synthesized. It was employed to investigate the binding in- teraction with the bovine serum albumin (BSA) in detail using different spectroscopic methods. It ex- hibited antibacterial activity against Escherichia cali and Staphylococcus aureus which are common food poisoning bacteria. The experimental results showed that the fluorescence quenching of model carrier protein BSA by AHDMAPPC was due to static quenching. The site binding constants and number of binding sites (n ≈ 1) were determined at three different temperatures based on fluorescence quenching results. The thermodynamic parameters, enthalpy change (AH), free energy (AG) and entropy change (AS) for the reaction were calculated to be 15.15 kJ/mol, -36.11 kJ/mol and 51.26J/mol K according to van't Hoff equation, respectively. The results indicated that the reaction was an endothermic and spontaneous process, and hydrophobic interactions played a major role in the binding between drug and BSA. The distance between donor and acceptor is 2.79 nm according to Forster's theory. The alterations of the BSA secondary structure in the presence of AHDMAPPC were confirmed by UV-visible, synchronous fluorescence, circular dichroism (CD) and three-dimensional fluorescence spectra. All these results in- dicated that AHDMAPPC can bind to BSA and be effectively transported and eliminated in the body. It can be a useful guideline for further drug design. 展开更多
关键词 Bovine serum albumin Fluorescence spectroscopy Pyrimidine derivative Binding interaction Fluorescence resonance energy transfer(FRET)
下载PDF
Investigation of the interaction between indigotin and two serum albumins by spectroscopic approaches 被引量:4
5
作者 Zheng-Jun Cheng Hong-Mei Zhao +1 位作者 Qian-Yong Xu Rong Liu 《Journal of Pharmaceutical Analysis》 SCIE CAS 2013年第4期257-269,共13页
The binding characteristics of indigotin with human serum albumin (HSA) and bovine serum albumin (BSA) have been investigated by various spectroscopic techniques. Spectroscopic analysis revealed that the quenching... The binding characteristics of indigotin with human serum albumin (HSA) and bovine serum albumin (BSA) have been investigated by various spectroscopic techniques. Spectroscopic analysis revealed that the quenching mechanism between indigotin and HSA/BSA belonged to the static quenching. The displacement experiments suggested that indigotin primarily bound to tryptophan residues on proteins within site I. The thermodynamic parameters indicated that the binding of indigoti^HSA/BSA mainly depended on the hydrophobic interaction. The binding distance of indigotin to HSA/BSA was evaluated. The results by synchronous fluorescence, three- dimensional fluorescence, Fourier Transform Infrared spectroscopy (FT-IR) and circular dichroism (CD) spectra showed that the conformation of proteins altered in the presence of indigotin. 展开更多
关键词 Human serm albumin Bovine serum albumin Indigoiin Fluorescence spectro-scopy Binding constants
下载PDF
Study of <i>in Vitro</i>Interaction of Sildenafil Citrate with Bovine Serum Albumin by Fluorescence Spectroscopy 被引量:6
6
作者 Md. Abdus Salam Md. Rokonujjaman +2 位作者 Asma Rahman Ummay Nasrin Sultana Md. Zakir Sultan 《Pharmacology & Pharmacy》 2015年第2期94-101,共8页
In vitro interaction of sildenafil citrate (SC) with bovine serum albumin (BSA) was investigated at two excitation wavelengths of BSA (280 nm and 293 nm) at two different temperatures (298 K and 308 K) by fluorescence... In vitro interaction of sildenafil citrate (SC) with bovine serum albumin (BSA) was investigated at two excitation wavelengths of BSA (280 nm and 293 nm) at two different temperatures (298 K and 308 K) by fluorescence emission spectroscopy. The study showed that quenching of BSA fluores-cence by sildenafil citrate was the result of formation BSA-SC complex with probable involvement of both tryptophan and tyrosine residues of BSA. Fluorescence quenching constant was determined from Stern-Volmer equation, and both static quenching and dynamic quenching were showed for BSA by SC at the conditions. Van’t Hoff equation was used to measure the thermodynamic parameters ΔG, ΔH, and ΔS at the temperatures which indicated that the hydrogen bond and the hydrophobic forces played major roles for BSA-SC complexation. The binding number (n) was found to be ≈1 indicating that one mole BSA bound with one mole SC. The binding affinity of SC to BSA was calculated at different temperatures. The binding constant was decreased with increasing temperatures indicating that stability of BSA-SC complex decreased with increasing temperatures. 展开更多
关键词 SILDENAFIL CITRATE Bovine serum albumin Quenching Fluorescence Spectroscopy
下载PDF
Immobilization of Bovine Serum Albumin on Poly(ether ether ketone) for Surface Biocompatibility Improvement 被引量:4
7
作者 SUN Hui CHEN Rui-chao +1 位作者 LI Ang XU Guo-zhi 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2012年第2期353-357,共5页
UV-induced graft polymerization of acrylic acid(AA) on poly(ether ether ketone)(PEEK) films was carried out to introduce ―COOH for the subsequent immobilization of bovine serum albumin(BSA).BSA was introduced... UV-induced graft polymerization of acrylic acid(AA) on poly(ether ether ketone)(PEEK) films was carried out to introduce ―COOH for the subsequent immobilization of bovine serum albumin(BSA).BSA was introduced on PEEK surface based on the condensation reaction between ―NH 2 and ―COOH.The modified surface(PEEK-BSA) was characterized by energy-disperse spectrometry(EDS),X-ray photoelectron spectroscopy(XPS),water contact angle measurement and UV spectrum analysis.The contact angle was found to decrease from 104° for the virgin PEEK films to 63° for the BSA-immobilized PEEK films,demonstrating a significant improvement of surface hydrophilicity.Moreover,the appearance of nitrogen on PEEK film confirmed by XPS and EDS indicates the immobilization of BSA on PEEK surface. 展开更多
关键词 Poly(ether ether ketone)(PEEK) UV-grafting Bovine serum albumin immobilization
下载PDF
Synthesis of Metal Porphyrins Tailed with Salicylic Acid and their Interaction with Bovine Serum Albumin 被引量:2
8
作者 TaoJIA KaiWANG +1 位作者 YiMeiZHAO ZaoYingLI 《Chinese Chemical Letters》 SCIE CAS CSCD 2004年第3期357-360,共4页
A synthetic method of porphyrins tailed with salicylic substituents is described. Reaction of bromoalkoxyphenyl porphyrin 1 with salicylic acid gave porphyrins 2~5. These new compounds were confirmed by 1H NMR, IR, ... A synthetic method of porphyrins tailed with salicylic substituents is described. Reaction of bromoalkoxyphenyl porphyrin 1 with salicylic acid gave porphyrins 2~5. These new compounds were confirmed by 1H NMR, IR, UV-vis, MS and elemental analysis, and observed their interaction with bovine serum albumin (BSA) in fluorescence spectrum. 展开更多
关键词 METALLOPORPHYRINS salicylic acid synthesis bovine serum albumin fluorescence.
下载PDF
Sonocatalytic Damage of Bovine Serum Albumin (BSA) in the Presence of Nanometer Titanium Dioxide (TiO_2) Catalyst 被引量:3
9
作者 Jun WANG Jing WU +6 位作者 Zhao Hong ZHANG Xiang Dong ZHANG Lei WANG Liang XU Bao Dong GUO Hong LI Jian TONG 《Chinese Chemical Letters》 SCIE CAS CSCD 2005年第8期1105-1108,共4页
The sonocatalytic damage of bovine serum albumin (BSA) was studied in the presence of nanometer titanium dioxide (TiO2) powders by low frequency (80 kHz) ultrasound. The destruction of secondary structure and ch... The sonocatalytic damage of bovine serum albumin (BSA) was studied in the presence of nanometer titanium dioxide (TiO2) powders by low frequency (80 kHz) ultrasound. The destruction of secondary structure and change of α-helical structure of BSA were reflected by ultraviolet (UV) and circular dichroism (CD) spectroscopies. 展开更多
关键词 Bovine serum albumin (BSA) sonocatalytic damage nanometer titanium dioxide (TiO2).
下载PDF
Dynamic Behaviors and Morphology Change of Anionic Phospholipid DPPG Monolayer Caused by Bovine Serum Albumin at Air-Water Interface
10
作者 徐国庆 郝长春 +2 位作者 张蕾 陈时 孙润广 《Chinese Journal of Chemical Physics》 SCIE CAS CSCD 2017年第5期595-602,I0002,共9页
The interaction between bovine serum albumin (BSA) and the anionic 1.2-dipalmitoyl-snglycero- 3-(phospho-rac-(1-glycerol)) (sodium salt) (DPPG) phospholipid at different subphase pH values was investigated a... The interaction between bovine serum albumin (BSA) and the anionic 1.2-dipalmitoyl-snglycero- 3-(phospho-rac-(1-glycerol)) (sodium salt) (DPPG) phospholipid at different subphase pH values was investigated at air-water interface through surface pressure measurements and atomic force microscopy (AFM) observation. By analyzing surface pressure-mean molecular area (π-A) isotherms, the limiting molecular area in the closed packing state-the concentration of BSA (Alim-[BSA]) curves, the compressibility coefficient-surface pressure (CS-1-π) curves and the difference value of mean molecular area-the concentration of BSA (ΔA-[BSA]) curves, we obtained that the mean molecular area of DPPG monolayer became much larger when the concentration of BSA in the subphase increased at pH=3 and 5. But the isotherms had no significant change at different amount of BSA at pH=10. In addition, the amount of BSA molecules adsorbed onto the lipid monolayer reached a threshold value when [BSA]〉5×10-8 mol/L for all pHs. From the surface pressure-time (π-t) data, we obtained that desorption and adsorption processes occurred at pH=3, however, there was only desorption process occurring at pH=5 and 10. These results showed that the interaction mechanism between DPPG and BSA molecules was affected by the pH of subphase. BSA molecules were adsorbed onto the DPPG monolayers mainly through the hydrophobic interaction at pH=3 and 5, and the strength of hydrophobic interaction at pH=3 was stronger than the case of pH=5. At pH=10, a weaker hydrophobic interaction and a stronger electrostatic repulsion existed between DPPG and BSA molecules. AFM images revealed that the pH of subphase and [BSA] could affect the morphology features of the monolayers, which was consistent with these curves. The study provides an important experimental basis and theoretical support to understand the interaction between lipid and BSA at the air-water interface. 展开更多
关键词 Bovine serum albumin DPPG Isotherm Interaction
下载PDF
Determination of Enantiomeric Compositions of Tryptophan by Chemometric Analysis of the Fluorescence Spectra of Bovine Serum Albumin Receptor-ligand Mixtures 被引量:2
11
作者 Yun Xia WANG Fang ZHANG +2 位作者 Jing LIANG Hua LI Ji Lie KONG 《Chinese Chemical Letters》 SCIE CAS CSCD 2006年第12期1599-1602,共4页
In this work, a novel method was constructed to determine the enantiomeric composition of tryptophan (Trp) by bovine serum albumin (BSA) based on the fluorescence spectra of the receptor-ligand mixtures coupled wi... In this work, a novel method was constructed to determine the enantiomeric composition of tryptophan (Trp) by bovine serum albumin (BSA) based on the fluorescence spectra of the receptor-ligand mixtures coupled with partial least squares (PLS-1) analysis. As a result the enantiomeric composition of Trp was accurately determined. 展开更多
关键词 Bovine serum albumin TRYPTOPHAN PLS-1 fluorescence spectrophotometry.
下载PDF
Antioxidant activity of bovine serum albumin binding amino acid Schiff-bases metal complexes 被引量:2
12
作者 Rong Min Wang Juan Juan Mao Jing Feng Song Cai Xia Huo Yu Feng He 《Chinese Chemical Letters》 SCIE CAS CSCD 2007年第11期1416-1418,共3页
Glutamic acid-salicylaldehyde Schiff-base metal complexes are bound into bovine serum albumin (BSA), which afforded BSA binding Schiff-base metal complexes (BSA-SalGluM, M=Cu, Co, Ni, Zn). The BSA binding metal co... Glutamic acid-salicylaldehyde Schiff-base metal complexes are bound into bovine serum albumin (BSA), which afforded BSA binding Schiff-base metal complexes (BSA-SalGluM, M=Cu, Co, Ni, Zn). The BSA binding metal complexes were characterized by UV-vis spectra and Native PAGE. It showed that the protein structures of BSA kept after coordinating amino acid Schiff-bases metal complexes. The effect of the antioxidant activity was investigated. The results indicate that the antioxidant capacity of BSA increased more than 10 times after binding Schiff-base metal complexes. 展开更多
关键词 Natural polymer Schiff-base metal complexes Glutamic acid Bovine serum albumin (BSA) ANTIOXIDANTS Hydroxyl radicals
下载PDF
Using capillary electrophoresis mobility shift assay to study the interaction of CdTe quantum dots with bovine serum albumin 被引量:2
13
作者 Li Wen Shao Chao Qing Dong Xiang Yi Huang Ji Cun Ren 《Chinese Chemical Letters》 SCIE CAS CSCD 2008年第6期707-710,共4页
In this work, the capillary electrophoresis mobility shift assay (CEMSA) was first adopted to study the interaction of protein with quantum dots (QDs). In this study, bovine serum albumin (BSA) and CdTe QDs were... In this work, the capillary electrophoresis mobility shift assay (CEMSA) was first adopted to study the interaction of protein with quantum dots (QDs). In this study, bovine serum albumin (BSA) and CdTe QDs were used as model samples. We observed that BSA was facilely adsorbed to CdTe QDs surface, and the QD-BSA complex was formed by a 1:1 stoichiometric ratio. A value of 2.17 4-0.27 × 10^6 mol^-1 L^-1 (at 25 ℃) for the association constant was obtained by CEMSA. 展开更多
关键词 CdTe quantum dots Bovine serum albumin Capillary electrophoresis mobility shift assay Interaction
下载PDF
Characterization of Interaction Between Raltitrexed and Bovine Serum Albumin by Optical Spectroscopic Techniques 被引量:2
14
作者 ZHANG Jia-xing YIN Zong-ning +3 位作者 WU Wei WANG Zhong-xia HE Rui WU Zhao-xu 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2012年第6期963-970,共8页
The interaction of raltitrexed(RTX) with bovine serum albumin(BSA) was investigated by steady state/lifetime fluorescence spectroscopy and circular dichroism(CD) spectroscopy under the simulative physiological c... The interaction of raltitrexed(RTX) with bovine serum albumin(BSA) was investigated by steady state/lifetime fluorescence spectroscopy and circular dichroism(CD) spectroscopy under the simulative physiological conditions.The results of fluorescence titration reveal that RTX could strongly quench the intrinsic fluorescence of BSA via a static quenching procedure.The obtained binding constant K A of RTX with BSA was 478630 and 44259 L/mol at 298 and 310 K,respectively.According to van't Hoff equation,the thermodynamic parameters ΔH,ΔG and ΔS were calculated,indicating that hydrophobic forces were the predominant intermolecular forces in stabilizing the complex.The binding process was a spontaneous process,in which Gibbs free energy change was negative.According to F rster's non-radioactive energy transfer theory,the distance r between donor(BSA) and acceptor(RTX) was 3.82 nm,suggesting that the energy transfer from BSA to RTX occurred with high probability.Displacement experiment and the number of binding sites calculation confirmed that RTX could bind to the site-I of BSA.Furthermore,the effects of pH and some metal ions on the interaction of RTX with BSA were also investigated.The results of synchronous fluorescence and CD spectra show that the RTX-BSA binding induced conformational changes in BSA. 展开更多
关键词 Raltitrexed(RTX) Bovine serum albumin(BSA) Fluorescence quenching Fluorescence lifetime Circular dichroism(CD) spectroscopy
下载PDF
Effect of PEGylation on the physicochemical and pharmacokinetic characteristics of bovine serum albumin-encapsulated liposome 被引量:2
15
作者 Yuko Okamoto Kazuaki Taguchi +3 位作者 Keishi Yamasaki Mina Sakuragi Shun’ichi Kuroda Masaki Otagiri 《Asian Journal of Pharmaceutical Sciences》 SCIE CAS 2016年第1期112-113,共2页
There is now little doubt that PEGylation is useful and is in widespread use because it provides a prolonged half-life,a higher stability and a lowerimmunogenicity[1].However,it is of concern that PEGylation may affec... There is now little doubt that PEGylation is useful and is in widespread use because it provides a prolonged half-life,a higher stability and a lowerimmunogenicity[1].However,it is of concern that PEGylation may affect the physicochemical and pharmacokinetic characteristics of protein-encapsulated liposome.Thus,we prepared the bovine serum albumin(BSA)-encapsulated liposome(BSA-liposome)with or without PEG and then compared their physicochemical and pharmacokinetic characteristics(Fig.1).BSA-liposomes were prepared by the thin-film hydration method. 展开更多
关键词 LIPOSOME PEGYLATION BOVINE serum albumin
下载PDF
Denaturation studies on bovine serum albumin–bile salt system:Bile salt stabilizes bovine serum albumin through hydrophobicity 被引量:1
16
作者 Karpagaraj Malarkani Ivy Sarkar Susithra Selvama 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2018年第1期27-36,共10页
Protein denaturation is under intensive research, since it leads to neurological disorders of severe consequences. Avoiding denaturation and stabilizing the proteins in their native state is of great importance,especi... Protein denaturation is under intensive research, since it leads to neurological disorders of severe consequences. Avoiding denaturation and stabilizing the proteins in their native state is of great importance,especially when proteins are used as drug molecules or vaccines. It is preferred to add pharmaceutical excipients in protein formulations to avoid denaturation and thereby stabilize them. The present study aimed at using bile salts(BSs), a group of well-known drug delivery systems, for stabilization of proteins.Bovine serum albumin(BSA) was taken as the model protein, whose association with two BSs, namely sodium cholate(Na C) and sodium deoxycholate(Na DC), was studied. Denaturation studies on the preformed BSA-BS systems were carried out under chemical and physical denaturation conditions. Urea was used as the chemical denaturant and BSA-BS systems were subjected to various temperature conditions to understand the thermal(physical) denaturation. With the denaturation conditions prescribed here,the data obtained is informative on the association of BSA-BS systems to be hydrophobic and this effect of hydrophobicity plays an important role in stabilizing the serum albumin in its native state under both chemical and thermal denaturation. 展开更多
关键词 BILE salts Bovine serum albumin Chemical DENATURATION Thermal DENATURATION TRYPTOPHAN fluorescence Protein stabilization
下载PDF
A spectroscopic study of the interaction of the antioxidant naringin with bovine serum albumin 被引量:5
17
作者 Atanu Singha Roy Debi Ranjan Tripathy +1 位作者 Angshuman Chatterjee Swagata Dasgupta 《Journal of Biophysical Chemistry》 2010年第3期141-152,共12页
The interaction of naringin with bovine serum albumin has been performed using fluorescence, circular dichroism and fourier transform infrared spectroscopy in 20 mM phosphate buffer of pH 7.0 as well as molecular dock... The interaction of naringin with bovine serum albumin has been performed using fluorescence, circular dichroism and fourier transform infrared spectroscopy in 20 mM phosphate buffer of pH 7.0 as well as molecular docking studies. The changes in enthalpy (ΔH°) and entropy (ΔS°) of the interaction were found to be +18.73 kJ/mol and +143.64 J mol-1 K-1 respectively, indicating that the interaction of naringin with bovine serum albumin occurred mainly through hydrophobic interactions. Negative values of free energy change (ΔG°) at different temperatures point toward the spontaneity of the interaction. Circular dichroism studies reveal that the helical content of bovine serum albumin decreased after interaction with naringin. According to the F?rster non-radiative energy transfer theory the distance between Trp 213 residue and naringin was found to be 3.25 nm. Displacement studies suggest that naringin binds to site 1 (subdomain IIA) of bovine serum albumin (BSA) which was also substantiated by molecular docking studies. 展开更多
关键词 NARINGIN BOVINE serum albumin Fluorescence Binding WARFARIN DOCKING
下载PDF
Binding interactions of pefloxacin mesylate with bovine lactoferrin and human serum albumin 被引量:2
18
作者 FAN Ji-cai CHEN Xiang WANG Yun FAN Cheng-ping SHANG Zhi-cai 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2006年第6期452-458,共7页
The binding of pefloxacin mesylate (PFLX) to bovine lactoferrin (BLf) and human serum albumin (HSA) in dilute aqueous solution was studied using fluorescence spectra and absorbance spectra. The binding constant ... The binding of pefloxacin mesylate (PFLX) to bovine lactoferrin (BLf) and human serum albumin (HSA) in dilute aqueous solution was studied using fluorescence spectra and absorbance spectra. The binding constant K and the binding sites n were obtained by fluorescence quenching method. The binding distance r and energy-transfer efficiency E between pefloxacin mesylate and bovine lactoferrin as well as human serum albumin were also obtained according to the mechanism of Forster-type dipole-dipole nonradiative energy-transfer. The effects of pefloxacin mesylate on the conformations of bovine lactoferrin and human serum albumin were also analyzed using synchronous fluorescence spectroscopy. 展开更多
关键词 Pefloxacin mesylate (PFLX) Bovine lactoferrin (BLf) Human serum albumin (HSA) Fluorescence spectra Energy-transfer efficiency
下载PDF
Morphological characterization of metal porphyrin tailed with aspirin interacting with bovine serum albumin congeries 被引量:1
19
作者 Kai Wang Lei Wu +1 位作者 Xiu Lan Zhang Zao Ying Li 《Chinese Chemical Letters》 SCIE CAS CSCD 2007年第12期1455-1459,共5页
The porphyrins tailed with acetylsalicylic acid (ASA) and Zn (or Cu) complexes were prepared. Meanwhile, morphological images, such as shape and size of porphyrins-BSA congeries were observed by using atomic force... The porphyrins tailed with acetylsalicylic acid (ASA) and Zn (or Cu) complexes were prepared. Meanwhile, morphological images, such as shape and size of porphyrins-BSA congeries were observed by using atomic force microscopy (AFM). The result showed the interaction of BSA and prepared porphyrins led to obvious change of shape and size of BSA congeries. 展开更多
关键词 PORPHYRIN Acetylsalicylic acid Bovine serum albumin Atomic force microscopy
下载PDF
Binding of naturally occurring hydroxycinnamic acids to bovine serum albumin 被引量:2
20
作者 Lucie Trnkova Iva Bousova +1 位作者 Vladimir Kubicek Jaroslav Drsata 《Natural Science》 2010年第6期563-570,共8页
Hydroxycinnamic acids (HCAs) possess numer- ous biological effects including antioxidant, antiallergic, antimicrobial, and immunomodulatory activities and due to these properties are widely used in folk medicine. Neve... Hydroxycinnamic acids (HCAs) possess numer- ous biological effects including antioxidant, antiallergic, antimicrobial, and immunomodulatory activities and due to these properties are widely used in folk medicine. Nevertheless, they can interact with protein molecules and cause some structural and functional changes. The possib- ility of HCAs binding to bovine serum albumin (BSA) under physiological conditions was inve- stigated by the UV-VIS absorption spectroscopy and fluorescence quenching method. Apart from rosmarinic acid, all tested HCAs quenched tryptophan fluorescence of BSA in the studied range of concentrations (0-20 μM) mainly by static quenching mechanism (formation of non- fluorescent HCA-BSA complexes). The binding constants, number of binding sites and free energy changes were determined. The binding affinities of HCAs were ranked in the order: chlorogenic acid > sinapic acid ≥ caffeic acid > ferulic acid > o-coumaric acid > p-coumaric acid ≥ m-coumaric acid, which was confirmed by spectral overlaps of BSA emission spectrum with absorption spectrum of HCA. All free energy changes possessed negative sign indicating the spontaneity of HCA-BSA interaction. 展开更多
关键词 BOVINE serum albumin Hydroxycinnamic Acid Fluorescence QUENCHING Protein-Ligand BINDING
下载PDF
上一页 1 2 119 下一页 到第
使用帮助 返回顶部