A bacterium classified as Achromobacter xylosoxidans strain IR08 by phenotypic typing coupled with 16S rRNA gene analysis was isolated from a soil contaminated with electrical transformer ?uid for over sixty years usi...A bacterium classified as Achromobacter xylosoxidans strain IR08 by phenotypic typing coupled with 16S rRNA gene analysis was isolated from a soil contaminated with electrical transformer ?uid for over sixty years using Aroclor 1221 as an enrichment substrate. The substrate utilization profiles revealed that IR08 could grow on all three monochlorobiphenyls (CBs), 2,4’- and 4,4’-dichlorobiphenyl as well as 2-chlorobenzoate (2-CBA), 3-CBA, 4-CBA, and 2,3-dichlorobenzoate. Unusually, growth was poorly sustaine...展开更多
High performance liquid chromatography method for the separation of a series of chiral benzyl alcohols on N-(3,5-dinitrobenzoyl)-D-phenylglycine stationary phase (Macherey Nagel, Chiral-2) after pre-column achiral der...High performance liquid chromatography method for the separation of a series of chiral benzyl alcohols on N-(3,5-dinitrobenzoyl)-D-phenylglycine stationary phase (Macherey Nagel, Chiral-2) after pre-column achiral derivatization was developed. Cheap and easy available aromatic acid chlorides were used as derivatization agents. Good to excellent separations of the enantiomers were achieved in all cases in relatively short analytical runs. It was shown that the enantiorecognition depends on the substituents both in the starting alcohol and in the acid chloride. The method presents an efficient alternative to the direct analyses on polysaccharide and cyclodextrine-derived stationary phases.展开更多
A study was conducted to compare the diversity of 2-, 3-, and 4-chlorobenzoate degraders in two pristine soils and one contaminated sewage sludge. These samples contained strikingly different populations of mono-chlor...A study was conducted to compare the diversity of 2-, 3-, and 4-chlorobenzoate degraders in two pristine soils and one contaminated sewage sludge. These samples contained strikingly different populations of mono-chlorobenzoate degraders. Although fewer cultures were isolated in the uncontaminated soils than contaminated one, the ability of microbial populations to mineralize chlorobenzoate was widespread. The 3- and 4-chlorobenzoate degraders were more diverse than the 2-chlorobenzoate degraders. One of the strains isolated from the sewage sludge was obtained. Based on its phenotype, chemotaxonomic properties and 16S rRNA gene, the organism S-7 was classified as Rhodococcus erythropolis. The strain can grow at temperature from 4 to 37℃. It can utilize several （halo）aromatic compounds. Moreover, strain S-7 can grow and use 3-chlorobenzoate as sole carbon source in a temperatures range of 10-30℃ with stoichiometric release of chloride ions. The psychrotolerant ability was significant for bioremediation in low temperature regions. Catechol and chlorocatechol 1,2-dioxygenase activities were present in cell free extracts of the strain, but no （chloro）catechol 2,3- dioxygenase activities was detected. Spectral conversion assays with extracts from R. erythropolis S-7 showed accumulation of a compound with a similar UV spectrum as chloro-cis,cis-muconate from 3-chlorobenzoate. On the basis of these results, we proposed that S-7 degraded 3-chlorobenzoate through the modified ortho-cleave pathway.展开更多
Densities of sodium chlorobenzoate( o , m , p ) were measured in solutions up to 80 weight percent dimethylformamide(DMF) at 298 15 K with an oscillating tube densimeter. From these densities, apparent molar v...Densities of sodium chlorobenzoate( o , m , p ) were measured in solutions up to 80 weight percent dimethylformamide(DMF) at 298 15 K with an oscillating tube densimeter. From these densities, apparent molar volumes of sodium chlorobenzoate in DMF H 2O mixtures were calculated, and partial molar volumes at infinite dilution were evaluated. Substituent and solvent effects on transfer volumes of each isomer from water to DMF H 2O mixed solvents were obtained. The results are explained in terms of solvent structure and solute solvent interaction.展开更多
Chlorobenzoic Acids are toxic organic compounds largely distributed in soils and sediments. They can be degraded to various products by microorgans. This paper is a review of the literature on biodegradability of the ...Chlorobenzoic Acids are toxic organic compounds largely distributed in soils and sediments. They can be degraded to various products by microorgans. This paper is a review of the literature on biodegradability of the chlorobenzoic acids. The degradation pathways, degradation genes, role of transposable elements, and construction of strains are discussed. A brief introduction is given on the environmental impacts and the pollution control.展开更多
Three kinds of novel hydroxyapatite@terbium complex core-shell composites were synthesized with 2-chlorobenzoic acid,4-chlorobenzoic acid,and 2,4-dichlorobenzoic acid as the ligand,respectively.The XRD,UV/vis absorpti...Three kinds of novel hydroxyapatite@terbium complex core-shell composites were synthesized with 2-chlorobenzoic acid,4-chlorobenzoic acid,and 2,4-dichlorobenzoic acid as the ligand,respectively.The XRD,UV/vis absorption and FT-IR results show that terbium ions are coordinated with ligands and the corresponding complexes are successfully included into composites.SEM and EDS results reveal that Tb complexes are fully and homogenously coated on the surface of hydroxyapatite(HAP)microspheres consisting of a large number of nanosized crystals.The strongest luminescence properties are obtained when 2,4-dichlorobenzoic acid is used as ligand.The study on fluorescence lifetime of composites shows that the lifetime is inversely related to emission intensity.TGA-DSC results indicate that the core-shell composites have an excellent thermal stability.Therefore,the current core-shell structure provides a cost-effective route to manufacture of biocompatible and heatresistant highly luminescent materials.展开更多
文摘A bacterium classified as Achromobacter xylosoxidans strain IR08 by phenotypic typing coupled with 16S rRNA gene analysis was isolated from a soil contaminated with electrical transformer ?uid for over sixty years using Aroclor 1221 as an enrichment substrate. The substrate utilization profiles revealed that IR08 could grow on all three monochlorobiphenyls (CBs), 2,4’- and 4,4’-dichlorobiphenyl as well as 2-chlorobenzoate (2-CBA), 3-CBA, 4-CBA, and 2,3-dichlorobenzoate. Unusually, growth was poorly sustaine...
文摘High performance liquid chromatography method for the separation of a series of chiral benzyl alcohols on N-(3,5-dinitrobenzoyl)-D-phenylglycine stationary phase (Macherey Nagel, Chiral-2) after pre-column achiral derivatization was developed. Cheap and easy available aromatic acid chlorides were used as derivatization agents. Good to excellent separations of the enantiomers were achieved in all cases in relatively short analytical runs. It was shown that the enantiorecognition depends on the substituents both in the starting alcohol and in the acid chloride. The method presents an efficient alternative to the direct analyses on polysaccharide and cyclodextrine-derived stationary phases.
文摘A study was conducted to compare the diversity of 2-, 3-, and 4-chlorobenzoate degraders in two pristine soils and one contaminated sewage sludge. These samples contained strikingly different populations of mono-chlorobenzoate degraders. Although fewer cultures were isolated in the uncontaminated soils than contaminated one, the ability of microbial populations to mineralize chlorobenzoate was widespread. The 3- and 4-chlorobenzoate degraders were more diverse than the 2-chlorobenzoate degraders. One of the strains isolated from the sewage sludge was obtained. Based on its phenotype, chemotaxonomic properties and 16S rRNA gene, the organism S-7 was classified as Rhodococcus erythropolis. The strain can grow at temperature from 4 to 37℃. It can utilize several （halo）aromatic compounds. Moreover, strain S-7 can grow and use 3-chlorobenzoate as sole carbon source in a temperatures range of 10-30℃ with stoichiometric release of chloride ions. The psychrotolerant ability was significant for bioremediation in low temperature regions. Catechol and chlorocatechol 1,2-dioxygenase activities were present in cell free extracts of the strain, but no （chloro）catechol 2,3- dioxygenase activities was detected. Spectral conversion assays with extracts from R. erythropolis S-7 showed accumulation of a compound with a similar UV spectrum as chloro-cis,cis-muconate from 3-chlorobenzoate. On the basis of these results, we proposed that S-7 degraded 3-chlorobenzoate through the modified ortho-cleave pathway.
文摘Densities of sodium chlorobenzoate( o , m , p ) were measured in solutions up to 80 weight percent dimethylformamide(DMF) at 298 15 K with an oscillating tube densimeter. From these densities, apparent molar volumes of sodium chlorobenzoate in DMF H 2O mixtures were calculated, and partial molar volumes at infinite dilution were evaluated. Substituent and solvent effects on transfer volumes of each isomer from water to DMF H 2O mixed solvents were obtained. The results are explained in terms of solvent structure and solute solvent interaction.
文摘Chlorobenzoic Acids are toxic organic compounds largely distributed in soils and sediments. They can be degraded to various products by microorgans. This paper is a review of the literature on biodegradability of the chlorobenzoic acids. The degradation pathways, degradation genes, role of transposable elements, and construction of strains are discussed. A brief introduction is given on the environmental impacts and the pollution control.
基金Project supported by the Major Project of Natural Science Research in Universities of Anhui Province(KJ2018ZD037,KJ2016A550)Horizontal Cooperation Project of Fuyang Municipal Government and Fuyang Normal University(XDHX201717,XDHX2016011,XDHX2016004)。
文摘Three kinds of novel hydroxyapatite@terbium complex core-shell composites were synthesized with 2-chlorobenzoic acid,4-chlorobenzoic acid,and 2,4-dichlorobenzoic acid as the ligand,respectively.The XRD,UV/vis absorption and FT-IR results show that terbium ions are coordinated with ligands and the corresponding complexes are successfully included into composites.SEM and EDS results reveal that Tb complexes are fully and homogenously coated on the surface of hydroxyapatite(HAP)microspheres consisting of a large number of nanosized crystals.The strongest luminescence properties are obtained when 2,4-dichlorobenzoic acid is used as ligand.The study on fluorescence lifetime of composites shows that the lifetime is inversely related to emission intensity.TGA-DSC results indicate that the core-shell composites have an excellent thermal stability.Therefore,the current core-shell structure provides a cost-effective route to manufacture of biocompatible and heatresistant highly luminescent materials.
