Response of two wheat cultivars （Triticum aestivum cv. YM 158 and NM 9） to the herbicide chlorotoluron and the effect of two forms of dissolved organic matter on the chlorotoluron toxicity to the plants were charact...Response of two wheat cultivars （Triticum aestivum cv. YM 158 and NM 9） to the herbicide chlorotoluron and the effect of two forms of dissolved organic matter on the chlorotoluron toxicity to the plants were characterized. Treatment with chlorotoluron at 10-50 μg/ml inhibited the seed germination and a dose-response was observed. The inhibition of seed germination was correlated to the depression of a-amylase activities. To identify whether chlorotoluron induced oxidative damage to wheat plants, the malondlaldehyde （MDA） content and electrolyte leakage were measured. Results showed that both MDA content and electrolyte leakage in the chlorotoluron-treated roots significantly increased. Activities of several key enzymes were measured that operate in citric acid cycle and carbohydrate metabolic pathway. Inhibited activities of citrate synthase and NADP-isocitrate dehydrogenase were observed in the chlorotoluron-treated roots as compared to control plants. We also examined malate dehydrogenase and phosphoenolpyruvate carboxylase in wheat roots exposed to 30 μg/ml chlorotoluron, liowever, none of the enzymes showed significant changes in activities. Application of 160 μg/ml dissolved organic matter （DOM） extracted from non-treated sludge（NTS） and heat-expanded sludge （lIES） in the medium with 30 μg/ml chlorotoluron induced an additive inhibition of seed germination and plant growth. The inhibition of growth due to the DOM treatment was associated with the depression of activities of a-amylase, citrate synthase and NADP-isocitrate dehydrogenase, as well as the increase in malondlaldehyde content and electrolyte leakage. These results suggested that the presence of DOM might enhance the uptake and accumulation of chlorotoluron, and thus resulted in greater toxicity in wheat plants. The two forms of DOM exhibited differences in regulation of chlorotoluron toxicity to the wheat plants. Treatments with DOM-NTS induced greater toxicity to plants as compared to those with DOM-HES. In addition to DOM affecting chlorotoluron-induced toxicity to wheat plants, the cultivars could have also contributed to differences. Generally, NM-9 showed a higher sensitivity to chlorotoluron than YM 158 either in the absence or in the presence of DOM.展开更多
Sorption of chlorotoluron in ammonium sulfate, urea and atrazine multi-solutes system was investigated by batch experiments. The results showed application of nitrogen fertilizers to the soil could affect the behavior...Sorption of chlorotoluron in ammonium sulfate, urea and atrazine multi-solutes system was investigated by batch experiments. The results showed application of nitrogen fertilizers to the soil could affect the behavior of chlorotoluron. At the same concentration of N, sorption of chlorotoluron decreased as the concentration of atrazine increased on the day 0 and 6 in soil, respectively. The sorption of chlorotoluron increased from 0 to 6 d when soils were preincubated with deionized water, ammonium sulfate and urea solution for 6 d. That indicated incubation time was one of the most important factors for the sorption of chlorotoluron in nitrogen fertilizers treatments. The individual sorption isotherms of chlorotoluron in rubbery polymer and silica were strictly linear in single solute system, but there were competition sorption between pesticides or between pesticides and nitrogen fertilizers. That indicated the sorption taken place by concurrent solid-phase dissolution mechanism and sorption on the interface of water-organic matter or water-mineral matter.展开更多
A batch equilibrium techniques was used to examine the effect of dissolved organic matter (DOM) extracted from both non-treated sludge (NTS) and heat-expanded sludge (HES) on the sorption and desorption of chlorotolur...A batch equilibrium techniques was used to examine the effect of dissolved organic matter (DOM) extracted from both non-treated sludge (NTS) and heat-expanded sludge (HES) on the sorption and desorption of chlorotoluron (3-(3- chloro-p-tolyl)-l,l-dimethylurea) in two types of soils, a yellow fluvo-aquic and a red soil from China. Without DOM, sorption of chlorotoluron was significantly greater (P < 0.05) in the red soil than in the yellow fluvo-aquic soil. However, with DOM the effect was dependent on the soil type and nature of DOM. Chlorotoluron sorption was lower in the yellow fluvo-aquic soil than in the red soil, suggesting that with the same DOM levels the yellow fluvo-aquic soil had a lower sorption capacity for this herbicide. Application of DOM from both NTS and HES led to a general decrease in sorption to the soils and an increase in desorption from the soils. Desorption of chlorotoluron also significantly increased (P < 0.05) with an increase in the DOM concentration. Additionally, for sorption and desorption, at each DOM treatment level the NTS treatments were significantly lower (P < 0.05) than the HES treatments. This implied that non-treated sludge had a greater effect on the sorption and desorption of chlorotoluron than heat-expanded sludge.展开更多
基金The National Natural Science Foundation of China（30170537）
文摘Response of two wheat cultivars （Triticum aestivum cv. YM 158 and NM 9） to the herbicide chlorotoluron and the effect of two forms of dissolved organic matter on the chlorotoluron toxicity to the plants were characterized. Treatment with chlorotoluron at 10-50 μg/ml inhibited the seed germination and a dose-response was observed. The inhibition of seed germination was correlated to the depression of a-amylase activities. To identify whether chlorotoluron induced oxidative damage to wheat plants, the malondlaldehyde （MDA） content and electrolyte leakage were measured. Results showed that both MDA content and electrolyte leakage in the chlorotoluron-treated roots significantly increased. Activities of several key enzymes were measured that operate in citric acid cycle and carbohydrate metabolic pathway. Inhibited activities of citrate synthase and NADP-isocitrate dehydrogenase were observed in the chlorotoluron-treated roots as compared to control plants. We also examined malate dehydrogenase and phosphoenolpyruvate carboxylase in wheat roots exposed to 30 μg/ml chlorotoluron, liowever, none of the enzymes showed significant changes in activities. Application of 160 μg/ml dissolved organic matter （DOM） extracted from non-treated sludge（NTS） and heat-expanded sludge （lIES） in the medium with 30 μg/ml chlorotoluron induced an additive inhibition of seed germination and plant growth. The inhibition of growth due to the DOM treatment was associated with the depression of activities of a-amylase, citrate synthase and NADP-isocitrate dehydrogenase, as well as the increase in malondlaldehyde content and electrolyte leakage. These results suggested that the presence of DOM might enhance the uptake and accumulation of chlorotoluron, and thus resulted in greater toxicity in wheat plants. The two forms of DOM exhibited differences in regulation of chlorotoluron toxicity to the wheat plants. Treatments with DOM-NTS induced greater toxicity to plants as compared to those with DOM-HES. In addition to DOM affecting chlorotoluron-induced toxicity to wheat plants, the cultivars could have also contributed to differences. Generally, NM-9 showed a higher sensitivity to chlorotoluron than YM 158 either in the absence or in the presence of DOM.
基金Project supported by the National Natural Science Foundation of China(No. 29837170).
文摘Sorption of chlorotoluron in ammonium sulfate, urea and atrazine multi-solutes system was investigated by batch experiments. The results showed application of nitrogen fertilizers to the soil could affect the behavior of chlorotoluron. At the same concentration of N, sorption of chlorotoluron decreased as the concentration of atrazine increased on the day 0 and 6 in soil, respectively. The sorption of chlorotoluron increased from 0 to 6 d when soils were preincubated with deionized water, ammonium sulfate and urea solution for 6 d. That indicated incubation time was one of the most important factors for the sorption of chlorotoluron in nitrogen fertilizers treatments. The individual sorption isotherms of chlorotoluron in rubbery polymer and silica were strictly linear in single solute system, but there were competition sorption between pesticides or between pesticides and nitrogen fertilizers. That indicated the sorption taken place by concurrent solid-phase dissolution mechanism and sorption on the interface of water-organic matter or water-mineral matter.
基金Project supported by the National Natural Science Foundation of China (No. 30170537).
文摘A batch equilibrium techniques was used to examine the effect of dissolved organic matter (DOM) extracted from both non-treated sludge (NTS) and heat-expanded sludge (HES) on the sorption and desorption of chlorotoluron (3-(3- chloro-p-tolyl)-l,l-dimethylurea) in two types of soils, a yellow fluvo-aquic and a red soil from China. Without DOM, sorption of chlorotoluron was significantly greater (P < 0.05) in the red soil than in the yellow fluvo-aquic soil. However, with DOM the effect was dependent on the soil type and nature of DOM. Chlorotoluron sorption was lower in the yellow fluvo-aquic soil than in the red soil, suggesting that with the same DOM levels the yellow fluvo-aquic soil had a lower sorption capacity for this herbicide. Application of DOM from both NTS and HES led to a general decrease in sorption to the soils and an increase in desorption from the soils. Desorption of chlorotoluron also significantly increased (P < 0.05) with an increase in the DOM concentration. Additionally, for sorption and desorption, at each DOM treatment level the NTS treatments were significantly lower (P < 0.05) than the HES treatments. This implied that non-treated sludge had a greater effect on the sorption and desorption of chlorotoluron than heat-expanded sludge.
