Bioremediation of organophosphorus pesticides in contaminated foodstuffs using probiotics has been increasingly under the spotlight in recent years,though the biodegradation mechanism and derived intermediate products...Bioremediation of organophosphorus pesticides in contaminated foodstuffs using probiotics has been increasingly under the spotlight in recent years,though the biodegradation mechanism and derived intermediate products remain unclear.This study aimed to help fill this knowledge gap and examined the degradation mechanism of organophosphorus pesticide,chlorpyrifos,in milk by Lactobacillus delbrueckii ssp.bulgaricus using gas chromatography-tandem mass spectrometry(GC-MS/MS)combined with transcriptome analysis.After the strain was cultured for 20 h in the presence of chlorpyrifos,differential expressions of 383 genes were detected,including genes probably implicated during chlorpyrifos degradation such as those related to hydrolase,phosphoesterase,diphosphatase,oxidoreductase,dehydratase,as well as membrane transporters.GC-MS/MS analysis revealed the changes of secondary metabolites in L.bulgaricus during milk fermentation due to chlorpyrifos stress.6-Methylhexahydro-2H-azepin-2-one,2,6-dihydroxypyridine and methyl 2-aminooxy-4-methylpentanoate as intermediates,along with the proposed pathways,might be involved in chlorpyrifos biodegradation by L.bulgaricus.展开更多
目的体外HepG2细胞培养观察芒果苷(mangiferin)对多耐药相关蛋白3(multidrug resistance-associate protein 3,MRP3)和核受体孕烷X受体(pregnane X receptor,PXR)、CYP7A启动子结合因子(CYP7A promoter-binding factor,CPF)表达的影响...目的体外HepG2细胞培养观察芒果苷(mangiferin)对多耐药相关蛋白3(multidrug resistance-associate protein 3,MRP3)和核受体孕烷X受体(pregnane X receptor,PXR)、CYP7A启动子结合因子(CYP7A promoter-binding factor,CPF)表达的影响。方法用芒果苷刺激HepG2细胞72 h后,分别抽提细胞RNA、膜蛋白及核蛋白,采用半定量RT-PCR和蛋白免疫印迹检测膜转运蛋白MRP3和核受体PXR、CPF在转录与蛋白水平的表达变化。熊脱氧胆酸(ursode-oxycholic acid,UDCA)处理的HepG2细胞作为阳性对照、DMSO处理细胞为阴性对照。结果芒果苷可显著刺激HepG2细胞膜转运蛋白MRP3的mRNA(比阴性对照组高3.0倍,P<0.05)和蛋白(比阴性对照组高3.3倍,P<0.05)表达,其作用强于UDCA。芒果苷也可明显上调核受体PXR[mRNA水平增高1.7倍(P<0.05),蛋白水平增高3.7倍(P<0.01)]、CPF[mRNA水平增高2.1倍(P<0.05),蛋白水平增高4.9倍(P<0.05)]的表达。结论芒果苷刺激肝癌细胞HepG2细胞膜转运蛋白MRP3的表达上调可能与核受体PXR、CPF途径相关。展开更多
[ Objective] The study aimed to discuss the toxicity effect of abamectin and ehlorpyrifos on grass carp （ Ctenopharyngdon idllus ） and their interrela- tions. [ Method] Taking healthy and active grass carp as the ob...[ Objective] The study aimed to discuss the toxicity effect of abamectin and ehlorpyrifos on grass carp （ Ctenopharyngdon idllus ） and their interrela- tions. [ Method] Taking healthy and active grass carp as the object, the acute toxicity test of single pesticide and the combined toxicity test of two pesticides on grass carp were carried out. [ Result] The LC50 of abamectin and chlorpyrifes and the mixture of two pesticides against grass carp at 24, 48, 72 and 96 h were as follows： abamectin： 0.54, 0.49, 0. 17 and 0.10 rag/L; chlorpyrifes ： 0.29, 0.21, 0.12 and 0.05 rag/L; the mixture of two pesticides ： 1.22, 1.08, 0.99 and 0. 86 mg/L. The safe concentration （SC） of ahamectin, chlorpyrifos and the mixture of two pesticides were 0.010, 0.005 and 0.086 rag/L, respectively. The tox- icity of the pesticides in sequence was ablorpyrifos 〉 ahamectin 〉 the mixture of two pesticides. [ Conclusion ] Different concentrations of abamectin, chlorpyrifos and the mixture of two pesticides had remarkable effect on the growth of grass carp, the higher the concentration was, the greater the toxicity effect was. Ahamectin at low toxicity intensity had certain relief function on the toxicity of ehlorpyrifos.展开更多
基金supported by Natural Science Foundation of China(41907357)Natural Science Foundation of Shandong(ZR2019PC048)the Key R&D project of Shandong Province(2021TZXD007).
文摘Bioremediation of organophosphorus pesticides in contaminated foodstuffs using probiotics has been increasingly under the spotlight in recent years,though the biodegradation mechanism and derived intermediate products remain unclear.This study aimed to help fill this knowledge gap and examined the degradation mechanism of organophosphorus pesticide,chlorpyrifos,in milk by Lactobacillus delbrueckii ssp.bulgaricus using gas chromatography-tandem mass spectrometry(GC-MS/MS)combined with transcriptome analysis.After the strain was cultured for 20 h in the presence of chlorpyrifos,differential expressions of 383 genes were detected,including genes probably implicated during chlorpyrifos degradation such as those related to hydrolase,phosphoesterase,diphosphatase,oxidoreductase,dehydratase,as well as membrane transporters.GC-MS/MS analysis revealed the changes of secondary metabolites in L.bulgaricus during milk fermentation due to chlorpyrifos stress.6-Methylhexahydro-2H-azepin-2-one,2,6-dihydroxypyridine and methyl 2-aminooxy-4-methylpentanoate as intermediates,along with the proposed pathways,might be involved in chlorpyrifos biodegradation by L.bulgaricus.
文摘目的体外HepG2细胞培养观察芒果苷(mangiferin)对多耐药相关蛋白3(multidrug resistance-associate protein 3,MRP3)和核受体孕烷X受体(pregnane X receptor,PXR)、CYP7A启动子结合因子(CYP7A promoter-binding factor,CPF)表达的影响。方法用芒果苷刺激HepG2细胞72 h后,分别抽提细胞RNA、膜蛋白及核蛋白,采用半定量RT-PCR和蛋白免疫印迹检测膜转运蛋白MRP3和核受体PXR、CPF在转录与蛋白水平的表达变化。熊脱氧胆酸(ursode-oxycholic acid,UDCA)处理的HepG2细胞作为阳性对照、DMSO处理细胞为阴性对照。结果芒果苷可显著刺激HepG2细胞膜转运蛋白MRP3的mRNA(比阴性对照组高3.0倍,P<0.05)和蛋白(比阴性对照组高3.3倍,P<0.05)表达,其作用强于UDCA。芒果苷也可明显上调核受体PXR[mRNA水平增高1.7倍(P<0.05),蛋白水平增高3.7倍(P<0.01)]、CPF[mRNA水平增高2.1倍(P<0.05),蛋白水平增高4.9倍(P<0.05)]的表达。结论芒果苷刺激肝癌细胞HepG2细胞膜转运蛋白MRP3的表达上调可能与核受体PXR、CPF途径相关。
基金Supported by Natural Science Foundation in Hunan Province(05JJ40035)~~
文摘[ Objective] The study aimed to discuss the toxicity effect of abamectin and ehlorpyrifos on grass carp （ Ctenopharyngdon idllus ） and their interrela- tions. [ Method] Taking healthy and active grass carp as the object, the acute toxicity test of single pesticide and the combined toxicity test of two pesticides on grass carp were carried out. [ Result] The LC50 of abamectin and chlorpyrifes and the mixture of two pesticides against grass carp at 24, 48, 72 and 96 h were as follows： abamectin： 0.54, 0.49, 0. 17 and 0.10 rag/L; chlorpyrifes ： 0.29, 0.21, 0.12 and 0.05 rag/L; the mixture of two pesticides ： 1.22, 1.08, 0.99 and 0. 86 mg/L. The safe concentration （SC） of ahamectin, chlorpyrifos and the mixture of two pesticides were 0.010, 0.005 and 0.086 rag/L, respectively. The tox- icity of the pesticides in sequence was ablorpyrifos 〉 ahamectin 〉 the mixture of two pesticides. [ Conclusion ] Different concentrations of abamectin, chlorpyrifos and the mixture of two pesticides had remarkable effect on the growth of grass carp, the higher the concentration was, the greater the toxicity effect was. Ahamectin at low toxicity intensity had certain relief function on the toxicity of ehlorpyrifos.
