AIM: To investigate the effects of gastrin and cholecystokinin (CCK) and their specific antagonists on the growth of pancreatic and biliary tract cancer cell lines. METHODS: Five pancreatic and 6 biliary cancer cell l...AIM: To investigate the effects of gastrin and cholecystokinin (CCK) and their specific antagonists on the growth of pancreatic and biliary tract cancer cell lines. METHODS: Five pancreatic and 6 biliary cancer cell lines with 2 conrtol cells were used in this study. Cell proliferation study was done using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) test and direct cell count method. Reverse transcription-polymerase chain reaction (RT-PCR) and slot blot hybridization were performed to examine and quantify the expression of hormonal receptors in these cell lines. RESULTS: SNU-308 showed a growth stimulating effect by gastrin-17, as did SNU-478 by both gastrin-17 and CCK-8. The trophic effect of these two hormones was completely blocked by specific antagonists (L-365, 260 for gastrin and L-364, 718 for CCK). Other cell lines did not respond to gastrin or CCK. In RT-PCR, the presence of CCK-A receptor and CCK-B/gastrin receptor mRNA was detected in all biliary and pancreatic cancer cell lines. In slot blot hybridization, compared to the cell lines which did not respond to hormones, those that responded to hormones showed high expression of receptor mRNA. CONCLUSION: Gastrin and CCK exert a trophic action on some of the biliary tract cancers.展开更多
The synthesis of CCK - 4 (H - Trp- Met- Asp- Phe- NH2 ) by using enzym es exclusively was described.As protection group for the amino group we used the Phenylacetyl group (Phac) which had been cleaved at the end of ...The synthesis of CCK - 4 (H - Trp- Met- Asp- Phe- NH2 ) by using enzym es exclusively was described.As protection group for the amino group we used the Phenylacetyl group (Phac) which had been cleaved at the end of the synthesis with Penicillin G Amidase (PGA ) without affecting the peptide bonds.Thus,beginning with Phac- Trp- OH we had successfully synthesized the target peptide with following4 enzymes,α- Chym otrypsin,Papain,Therm olysin and PGA in four reac- tion steps.All reactions were carried out in aqueous buffer in reasonable yields(>6 5 % ) .FAB- MS or FD- MS verified the correct molecular mass of all peptides.展开更多
Background Commercial diets are frequently formulated to meet or exceed nutrient levels including those of lim-iting essential amino acids(AA)covering potential individual variations within the herd.However,the provis...Background Commercial diets are frequently formulated to meet or exceed nutrient levels including those of lim-iting essential amino acids(AA)covering potential individual variations within the herd.However,the provision of dietary excess of AA,such as Lys,may lead to reduced appetite and growth in pigs.The mechanisms modulat-ing these responses have not been extensively investigated.This study evaluated the effect of Lys dietary excesses on performance and satiety biomarkers in post weaning pigs.Methods Twenty-four pigs aged 21 d and weighing 6.81±0.12kg(mean±SEM)were individually housed and offered 1 of 4 dietary treatments for 3weeks:a diet containing a standardized ileal digestible Lys reaching 100%(T0),120%(T1),150%(T2)or 200%(T3)of the NRC(2012)requirements.At the end of the experiment,blood samples from the cephalic vein of the T0 and T3 groups were obtained for AA analysis.In addition,primary intestinal cultures from T0 pigs were used,following their humane killing,to evaluate the effect of Lys on gut hormone secretion and AA sensors gene expression under ex vivo conditions.Results Feed intake was linearly reduced(P<0.001)and the weight gain to feed ratio reduced(P<0.10)with increased dietary levels of Lys during the third-and first-week post weaning,respectively.Cholecystokinin con-centration(P<0.05)and the metabotropic glutamate receptor 1 and the solute carrier family 7 member 2(P<0.10)gene expression was enhanced in proximal jejunum tissues incubated with Lys at 20mmol/L when compared to the control(Lys 0mmol/L).Plasma Lys and Glu(P<0.05)concentration increased in the T3 compared to T0 pigs.In contrast,plasma levels of His,Val,Thr,Leu(P<0.05)and Gln(P<0.10)were lower in T3 than T0 pigs.Conclusion The present results confirm that excess dietary Lys inhibits hunger in pigs.Moreover,the results provide evidence of pre-and post-absorptive mechanisms modulating these responses.Lys dietary excesses should be nar-rowed,when possible,to avoid negative effects of the AA on appetite in pigs.展开更多
全国知识图谱与语义计算大会和知识图谱国际联合会议联办,是由第十八届全国知识图谱与语义计算大会2024(China Conference on Knowledge Graph and Semantic Computing,CCKS 2024)和第十三届知识图谱国际联合会议2024(International Joi...全国知识图谱与语义计算大会和知识图谱国际联合会议联办,是由第十八届全国知识图谱与语义计算大会2024(China Conference on Knowledge Graph and Semantic Computing,CCKS 2024)和第十三届知识图谱国际联合会议2024(International Joint Conference of Knowledge Graph,IJCKG 2024)联合举办。展开更多
Biliary dyskinesia is a relatively common gastrointestinal disease that is increas-ing in incidence as living standards improve.However,its underlying pathogenesis remains unclear,hindering the development of therapeu...Biliary dyskinesia is a relatively common gastrointestinal disease that is increas-ing in incidence as living standards improve.However,its underlying pathogenesis remains unclear,hindering the development of therapeutic drugs.Recently,“Expression and functional study of cholecystokinin-A receptors on the interstitial Cajal-like cells of the guinea pig common bile duct”demonstrated that cholecystokinin(CCK)regulates the contractile function of the common bile duct through interaction with the CCK-A receptor in interstitial Cajal-like cells,contributing to improving the academic understanding of biliary tract dynamics and providing emerging directions for the pathogenesis and clinical management of biliary dyskinesia.This letter provides a brief overview of the role of CCK and CCK-A receptors in biliary dyskinesia from the perspective of animal experiments and clinical studies,and discusses prospects and challenges for the clinical application of CCK and CCK-A receptors as potential therapeutic targets.展开更多
全国知识图谱与语义计算大会(China Conference on Knowledge Graph and Semantic Computing,CCKS)由中国中文信息学会语言与知识计算专业委员会主办,大会源自中文知识图谱研讨会(Chinese Knowledge Graph Symposium,CKGS)和中国语义网...全国知识图谱与语义计算大会(China Conference on Knowledge Graph and Semantic Computing,CCKS)由中国中文信息学会语言与知识计算专业委员会主办,大会源自中文知识图谱研讨会(Chinese Knowledge Graph Symposium,CKGS)和中国语义网与万维网科学大会(Chinese Semantic Web and Web Science Conference,CSW),2016年两会合并,CCKS2016、2017、2018、2019、2020、2021、2022和2023分别在北京、成都、天津、杭州、南昌、广州(线上)、秦皇岛和沈阳举办。展开更多
2024年9月20日至22日,全国知识图谱与语义计算大会2024(China Conference on Knowledge Graph and Semantic Computing,CCKS 2024)暨知识图谱国际联合会议2024(International Joint Conference of Knowledge Graph,IJCKG 2024)在重庆举...2024年9月20日至22日,全国知识图谱与语义计算大会2024(China Conference on Knowledge Graph and Semantic Computing,CCKS 2024)暨知识图谱国际联合会议2024(International Joint Conference of Knowledge Graph,IJCKG 2024)在重庆举行,本次大会由中国中文信息学会语言与知识计算专委会主办,重庆大学承办,西南大学与重庆交通大学协办。展开更多
基金Supported by a grant from Seoul National University Research Fund (03-99-080 and 082)
文摘AIM: To investigate the effects of gastrin and cholecystokinin (CCK) and their specific antagonists on the growth of pancreatic and biliary tract cancer cell lines. METHODS: Five pancreatic and 6 biliary cancer cell lines with 2 conrtol cells were used in this study. Cell proliferation study was done using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) test and direct cell count method. Reverse transcription-polymerase chain reaction (RT-PCR) and slot blot hybridization were performed to examine and quantify the expression of hormonal receptors in these cell lines. RESULTS: SNU-308 showed a growth stimulating effect by gastrin-17, as did SNU-478 by both gastrin-17 and CCK-8. The trophic effect of these two hormones was completely blocked by specific antagonists (L-365, 260 for gastrin and L-364, 718 for CCK). Other cell lines did not respond to gastrin or CCK. In RT-PCR, the presence of CCK-A receptor and CCK-B/gastrin receptor mRNA was detected in all biliary and pancreatic cancer cell lines. In slot blot hybridization, compared to the cell lines which did not respond to hormones, those that responded to hormones showed high expression of receptor mRNA. CONCLUSION: Gastrin and CCK exert a trophic action on some of the biliary tract cancers.
文摘The synthesis of CCK - 4 (H - Trp- Met- Asp- Phe- NH2 ) by using enzym es exclusively was described.As protection group for the amino group we used the Phenylacetyl group (Phac) which had been cleaved at the end of the synthesis with Penicillin G Amidase (PGA ) without affecting the peptide bonds.Thus,beginning with Phac- Trp- OH we had successfully synthesized the target peptide with following4 enzymes,α- Chym otrypsin,Papain,Therm olysin and PGA in four reac- tion steps.All reactions were carried out in aqueous buffer in reasonable yields(>6 5 % ) .FAB- MS or FD- MS verified the correct molecular mass of all peptides.
基金This study was partially supported by The University of Queensland and Australian Pork Limited as part of the project APL 2016/053.
文摘Background Commercial diets are frequently formulated to meet or exceed nutrient levels including those of lim-iting essential amino acids(AA)covering potential individual variations within the herd.However,the provision of dietary excess of AA,such as Lys,may lead to reduced appetite and growth in pigs.The mechanisms modulat-ing these responses have not been extensively investigated.This study evaluated the effect of Lys dietary excesses on performance and satiety biomarkers in post weaning pigs.Methods Twenty-four pigs aged 21 d and weighing 6.81±0.12kg(mean±SEM)were individually housed and offered 1 of 4 dietary treatments for 3weeks:a diet containing a standardized ileal digestible Lys reaching 100%(T0),120%(T1),150%(T2)or 200%(T3)of the NRC(2012)requirements.At the end of the experiment,blood samples from the cephalic vein of the T0 and T3 groups were obtained for AA analysis.In addition,primary intestinal cultures from T0 pigs were used,following their humane killing,to evaluate the effect of Lys on gut hormone secretion and AA sensors gene expression under ex vivo conditions.Results Feed intake was linearly reduced(P<0.001)and the weight gain to feed ratio reduced(P<0.10)with increased dietary levels of Lys during the third-and first-week post weaning,respectively.Cholecystokinin con-centration(P<0.05)and the metabotropic glutamate receptor 1 and the solute carrier family 7 member 2(P<0.10)gene expression was enhanced in proximal jejunum tissues incubated with Lys at 20mmol/L when compared to the control(Lys 0mmol/L).Plasma Lys and Glu(P<0.05)concentration increased in the T3 compared to T0 pigs.In contrast,plasma levels of His,Val,Thr,Leu(P<0.05)and Gln(P<0.10)were lower in T3 than T0 pigs.Conclusion The present results confirm that excess dietary Lys inhibits hunger in pigs.Moreover,the results provide evidence of pre-and post-absorptive mechanisms modulating these responses.Lys dietary excesses should be nar-rowed,when possible,to avoid negative effects of the AA on appetite in pigs.
文摘全国知识图谱与语义计算大会和知识图谱国际联合会议联办,是由第十八届全国知识图谱与语义计算大会2024(China Conference on Knowledge Graph and Semantic Computing,CCKS 2024)和第十三届知识图谱国际联合会议2024(International Joint Conference of Knowledge Graph,IJCKG 2024)联合举办。
文摘Biliary dyskinesia is a relatively common gastrointestinal disease that is increas-ing in incidence as living standards improve.However,its underlying pathogenesis remains unclear,hindering the development of therapeutic drugs.Recently,“Expression and functional study of cholecystokinin-A receptors on the interstitial Cajal-like cells of the guinea pig common bile duct”demonstrated that cholecystokinin(CCK)regulates the contractile function of the common bile duct through interaction with the CCK-A receptor in interstitial Cajal-like cells,contributing to improving the academic understanding of biliary tract dynamics and providing emerging directions for the pathogenesis and clinical management of biliary dyskinesia.This letter provides a brief overview of the role of CCK and CCK-A receptors in biliary dyskinesia from the perspective of animal experiments and clinical studies,and discusses prospects and challenges for the clinical application of CCK and CCK-A receptors as potential therapeutic targets.
文摘全国知识图谱与语义计算大会(China Conference on Knowledge Graph and Semantic Computing,CCKS)由中国中文信息学会语言与知识计算专业委员会主办,大会源自中文知识图谱研讨会(Chinese Knowledge Graph Symposium,CKGS)和中国语义网与万维网科学大会(Chinese Semantic Web and Web Science Conference,CSW),2016年两会合并,CCKS2016、2017、2018、2019、2020、2021、2022和2023分别在北京、成都、天津、杭州、南昌、广州(线上)、秦皇岛和沈阳举办。
文摘2024年9月20日至22日,全国知识图谱与语义计算大会2024(China Conference on Knowledge Graph and Semantic Computing,CCKS 2024)暨知识图谱国际联合会议2024(International Joint Conference of Knowledge Graph,IJCKG 2024)在重庆举行,本次大会由中国中文信息学会语言与知识计算专委会主办,重庆大学承办,西南大学与重庆交通大学协办。
