In the present study we injected colchicine into the lateral ventricle of Sprague-Dawley rats to investigate the effects of colchicine on the number of different-type neurons in the basal forebrain and to search for n...In the present study we injected colchicine into the lateral ventricle of Sprague-Dawley rats to investigate the effects of colchicine on the number of different-type neurons in the basal forebrain and to search for neurons resistant to injury. After colchicine injection, the number of nestin^＋ cholinergic neurons was decreased at 1 day, but increased at 3 days and peaked at 14-28 days. The quantity of nestincholinergic neurons, parvalbumin-positive neurons and choline acetyl transferase-positive neurons decreased gradually. Our results indicate that nestin^＋ cholinergic neurons possess better tolerance to colchicine-induced neurotoxicity.展开更多
The present study established a rat model of chronic intermittent hypoxia （CIH） to simulate obstructive sleep apnea syndrome. CIH rats were evaluated for cognitive function using the Morris water maze, and neuronal ...The present study established a rat model of chronic intermittent hypoxia （CIH） to simulate obstructive sleep apnea syndrome. CIH rats were evaluated for cognitive function using the Morris water maze, and neuronal pathology in the hippocampus was observed using hematoxylin-eosin staining. In addition, hippocampal choline acetyl transferase （CHAT） and nicotinic acetylcholine receptor （nAChR） expression was determined by immunohistochemistry. Our results revealed necrotic hippocampal neurons, decreased ChAT and nAChR expression, as well as cognitive impairment in CIH rats. These results suggest that hippocampal neuronal necrosis and decreased cholinerqic activity may be involved in CIH-induced cognitive impairment in rats.展开更多
目的观察异丙酚预处理对拟阿尔茨海默病(AD)大鼠海马CA1区胆碱乙酰转移酶(cho- line acetyl-transferase,ChAT)表达的影响,分析异丙酚的脑保护作用。方法30只健康雄性SD大鼠随机分为对照组、拟AD模型组(简称模型组)和异丙酚预处理组(简...目的观察异丙酚预处理对拟阿尔茨海默病(AD)大鼠海马CA1区胆碱乙酰转移酶(cho- line acetyl-transferase,ChAT)表达的影响,分析异丙酚的脑保护作用。方法30只健康雄性SD大鼠随机分为对照组、拟AD模型组(简称模型组)和异丙酚预处理组(简称异丙酚组)。模型组、异丙酚预处理组在大鼠海马CA1区微量注射冈田酸(Okadaic acid,OA),建立拟AD大鼠模型。异丙酚预处理组大鼠于拟AD模型制作前30 min,腹腔注射异丙酚100 mg/kg。然后用免疫组化方法观察大鼠海马CA1区ChAT的表达。结果30只大鼠顺利完成全部实验的有27只,模型组和异丙酚预处理组有2只大鼠死亡、1只出现偏瘫无法进行余下实验,这可能与动物的个体差异不能耐受实验有关。随机取27只大鼠作为结果分析样本(每组9只)。①模型组与对照组大鼠相比:海马CA1区ChAT免疫组化阳性表达明显减少(P<0.05);②异丙酚预处理组与模型组大鼠相比:海马CA1区ChAT免疫组化阳性表达明显增多(P<0.05)。结论拟AD模型建立前30 min异丙酚预处理能增强ChAT的表达,有明显的脑保护作用。展开更多
基金the National Natural Science Foundation of China,No. 30700436
文摘In the present study we injected colchicine into the lateral ventricle of Sprague-Dawley rats to investigate the effects of colchicine on the number of different-type neurons in the basal forebrain and to search for neurons resistant to injury. After colchicine injection, the number of nestin^＋ cholinergic neurons was decreased at 1 day, but increased at 3 days and peaked at 14-28 days. The quantity of nestincholinergic neurons, parvalbumin-positive neurons and choline acetyl transferase-positive neurons decreased gradually. Our results indicate that nestin^＋ cholinergic neurons possess better tolerance to colchicine-induced neurotoxicity.
文摘The present study established a rat model of chronic intermittent hypoxia （CIH） to simulate obstructive sleep apnea syndrome. CIH rats were evaluated for cognitive function using the Morris water maze, and neuronal pathology in the hippocampus was observed using hematoxylin-eosin staining. In addition, hippocampal choline acetyl transferase （CHAT） and nicotinic acetylcholine receptor （nAChR） expression was determined by immunohistochemistry. Our results revealed necrotic hippocampal neurons, decreased ChAT and nAChR expression, as well as cognitive impairment in CIH rats. These results suggest that hippocampal neuronal necrosis and decreased cholinerqic activity may be involved in CIH-induced cognitive impairment in rats.
