CCL2 Enhances the Viability of Human Chorionic Trophoblast Cell Line HTR-8/SVneo Cells by Inhibiting Interleukin-24 Expression

Background:To investigate the regulatory effect of interleukin-24(IL-24)on cell viability of human chorionic trophoblast cell line(HTR-8/SVneo cells).Methods:Immunohistochemical staining was used to detect the expression of IL-24 and its receptors IL-20R1,IL-20R2,and IL-22R1 in villus tissue at early normal pregnancy.The effect of thymic stromal lymphopoietin(TSLP)and chemokine CCL2 on the expression of IL-24 in human chorionic trophoblast cell line HTR-8/SVneo cells was analyzed by In-cell Western.In addition,the effect of recombinant human IL-24(rhIL-24)and CCL2 on the viability of HTR-8/SVneo cells was analyzed by MTT assay.Results:IL-24 and its receptors showed a strong positive staining in trophoblasts at early normal pregnancy.Compared with control group,expression of IL-24 in HTR-8/SVneo cells was significantly inhibited after in vitro stimulation of recombinant human CCL2 protein(rhCCL2)(P<0.001).The viability of HTR-8/SVneo cells was significantly decreased after treatment with rhIL-24(P<0.001).In contrast,anti-IL-24 neutralizing antibody significantly enhanced the viability of HTR-8/SVneo cells(P<0.01).In addition,rhCCL2(100μg/L);enhanced the viability of HTR-8/SVneo cells(P<0.01)in vitro,but this effect was inhibited by treatment with rhIL-24.Conclusions:CCL2 enhances the viability of human trophoblast cell line HTR-8/SVneo cells in vitro by inhibiting the secretion of IL-24,which may be beneficial to blastocyst implantation and placental development.

Early detection of persistent trophoblastic tumour by serum human chorionic gonadotrophin monitoring after molar pregnancy

Objective To study the outcome of a multi centred post molar pregnancy serum human chorionic gonadotrophin (hCG) surveillance programme. Methods Patients recruited into the multi centred post molar serum hCG surveillance programme between 1988 and 1996 were studied. The clinical data were obtained from medical records and computer database. Results There were 616 patients in the study. Twenty five (11%) of 224 patients with molar pregnancy and 28 (7%) of 392 patients with partial molar pregnancy were diagnosed to have persistent trophoblastic tumour (PTT) requiring chemotherapy. Of the 53 patients treated for PTT, 58.5% received intravenous methotrexate (MTX), 22.6% received both MTX and actinomycin D, and 19% received CHAMOC, a multiple chemotherapeutic regimen. Four patients receiving single drug had to change the regimen because of poor response, and all recovered and remained well. There were 3 cases of recurrence, one in each group of patients receiving single, dual or multiple agents. One patient died because of treatment complication. The rest were well with a mean follow up of 42 months. Conclusions Post molar serum hCG surveillance is important to detecting persistent trophoblastic activity. Early treatment of PTT by the appropriate chemotherapy has a near hundred percent cure and prevents the progression of PTT into more advanced trophoblastic malignancy such as choriocarcinoma.